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See detailCharacterization of growth hormone-binding protein in cattle plasma: prolactin-binding activity and 24-hour profile.
Massart, S.; Ban, A. M.; Renaville, Robert ULg et al

in Domestic Animal Endocrinology (1996), 13(1), 47-57

The purpose of this study was to characterize circulating growth hormone-binding proteins (GHBP) and prolactin-binding proteins (PRLBP) in cattle blood plasma. In particular, the 24-hr profile of these ... [more ▼]

The purpose of this study was to characterize circulating growth hormone-binding proteins (GHBP) and prolactin-binding proteins (PRLBP) in cattle blood plasma. In particular, the 24-hr profile of these molecules was investigated. The preincubation of bull plasma with iodinated bovine growth hormone (bGH) or bovine prolactin (bPRL), followed by gel filtration chromatography (Superdex 200; 1.6 x 60 cm column), resulted in the formation of essentially two complexes. The majority of [125I]bPRL eluted with the first one (M(r) approximately 600 kDa), whereas [125I]bGH mainly appeared in the second one (M(r) approximately 70 kDa). The fractions corresponding to these two peaks were analyzed by western ligand blotting (WLB), under reducing conditions. WLB revealed, respectively, 190-, 56-, 52-, and 28-kDa bands for the first peak and only 52- and 28-kDa bands for the second one. The nature of the 600-kDa peak is at present undetermined, but the 70-kDa one was previously identified as high-affinity GHBP. Displacement studies demonstrated that bGH and bPRL were both able to bind to this GHBP, because the bGH- and bPRL-binding activities of this protein could be saturated by an excess of either of these two hormones. This was indirectly confirmed by the close correlation (r = 0.615; P = 0.0001; n = 155) observed between plasma bGH- and bPRL-binding activities, because this correlation could suggest that both ligands are bound to the same proteins. The temporal concentrations of plasma GHBP were measured in samples collected at 20-min intervals for 24 hr from 8 young bulls. The evaluation of GHBP was realized by WLB, followed by densitometric analysis. Some fluctuations were observed, but these were not correlated with bGH release, even with a +/- 2-hr lag period. In summary, we found that bovine high-affinity GHBP binds not only bGH, but also bPRL. A second type of protein, of higher molecular weight, also binds these two hormones, but further investigations are needed to determine its nature. Finally, GHBP concentrations in cattle blood plasma apparently show fluctuations over a 24-hr period, but no correlation was found between these fluctuations and plasma growth hormone concentrations. [less ▲]

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See detailGrowth hormone, insulin-like growth factor-I and their plasma binding proteins in different cattle breeds.
Renaville, Robert ULg; Massart, Serge; Shojae, D. et al

in Zootecnica e Nutrizione Animale (1995), 21(2),

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See detailRole des somatomedines/insulin-like growth factors dans la croissance ponderale foetale.
Renaville, Robert ULg; Falaki, M.; Burny, A. et al

in Bulletin des Recherches Agronomiques de Gembloux (1994), 29(3),

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See detailEffects of immunization against somatostatin on growth performances and growth hormone - insulin-like growth factor I and insulin plasma levels in young bulls.
Renaville, Robert ULg; Sneyers, M.; Massart, Serge et al

in Zootecnica e Nutrizione Animale (1994), 20(4),

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See detailBovine Growth Hormone-Taqi Rflp And Quantitative Traits Breed Selected For Growth Performances And Meat Production
Sneyers, M.; Renaville, Robert ULg; Falaki, M. et al

in Archives Internationales de Physiologie, de Biochimie et de Biophysique (1994), 102(2),

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See detailTaqi Restriction-Fragment-Length-Polymorphisms For Growth-Hormone In Bovine Breeds And Their Association With Quantitative Traits
Sneyers, M.; Renaville, Robert ULg; Falaki, M. et al

in Growth Regulation (1994), 4(3),

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See detailChanges In The Hypophyseal-Gonadal Axis During The Onset Of Puberty In Young Bulls
Renaville, Robert ULg; Devolder, A.; Massart, Serge et al

in Journal of Reproduction and Fertility (1993), 99(2),

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See detailPresence Of Growth Hormone-Binding Proteins In Cattle Plasma And Milk
Devolder, A.; Renaville, Robert ULg; Sneyers, M. et al

in Journal of Endocrinology (1993), 138(1),

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See detailRegulation endocrinienne et manipulation de la croissance bovine
Renaville, Robert ULg; Sneyers, M.; Devolder, A. et al

in Cahiers Agricultures (1992), 1(2),

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