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See detailDouble radial immunodiffusion as a tool to identify pregnancy-associated glycoproteins in ruminant and nonruminant placentae
El Amiri, B.; Melo de Sousa, Noelita ULg; Mecif, K. et al

in Theriogenology (2003), 59(5-6), 1291-1301

Pregnancy-associated glycoproteins (PAGs) are antigens synthesized in the superficial layers of the ruminant trophoblast. Initially, they were identified either as proteins released into the maternal ... [more ▼]

Pregnancy-associated glycoproteins (PAGs) are antigens synthesized in the superficial layers of the ruminant trophoblast. Initially, they were identified either as proteins released into the maternal bloodstream (where they have applications in pregnancy diagnosis) (PAG1) or as molecules binding to the LH receptor (PAG2). In this study, double radial immunodiffusion was used to test the ability of antisera raised against different PAG molecules (bovine, ovine and caprine) to react with placental extracts from nonruminants (rabbit, cat, mouse, pig, and wild pig) and ruminants (cow, ewe, and goat). Placental extracts from all nonruminants tested except rabbit reacted with anti bovine PAG2 (anti-boPAG2). Extracts of ruminant placentas reacted with different antisera, confirming the expression of various PAG molecules. According to the time at which the placentas were collected (early or middle pregnancy), the reaction differed as regards the thickness, position, and number of precipitation lines, suggesting that PAG expression varies as pregnancy progresses. Bos indicus and Bos taurus placental extracts exhibited different reactions with anti-boPAG2: a single precipitation line in the former case and two lines in the latter. This suggests differential expression of boPAG2 related glycoproteins in these two subspecies. [less ▲]

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See detailPepsinogen and progesterone concentration during pregnancy in sows.
Banga-Mboko, H.; Thilman, P.; Desbuleux, H. et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2002), 6(1), 12-13

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See detailPurification and characterization of a pregnancy-associated glycoprotein (ovPAG-6) from sheep placenta removed between 66 to 100 days of gestation.
El Amiri, B.; Remy, B.; Melo de Sousa, Noelita ULg et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2002), 6(1), 12-13

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See detailPregnancy-associated glycoprotein profiles of 5 heifers measured by three radioimmunoassay systems.
Perenyi, Z.; Desbuleux, H.; Sulon, J. et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2002), 6(1), 6-7

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See detailEssais d’induction artificielle de la lactation chez des génisses âgées de 6 a 7 mois
Nchare, A.; Salaheddine, M.; Desbuleux, H. et al

in Annales de Médecine Vétérinaire (2001), 145(6, Suppl. S), 365-371

The rates of successful gene's manipulations are very low in domestic mammals. In addition the period between generations in cows is long. Artificial induction of lactation in young animals could improve ... [more ▼]

The rates of successful gene's manipulations are very low in domestic mammals. In addition the period between generations in cows is long. Artificial induction of lactation in young animals could improve manipulation yield for genes what are expressed in the udder, by anticipating the control of transgen expression in milk, a long time before the beginning of the milk production in normal condition. Using this method, the early selection of real transgenic animals would be possible. Between November 1999 and April 2000, lactation was induced in non-transgenic heifers aged from 6 to 7 months, in 2 trials based on short time hormonal treatment, with the goal of verifying the efficiency of the technique on immature heifers, and setting up a simple and reliable treatment scheme applicable on young animals. Interesting lactations (35 to 974 ml/day) were obtained, but the milk yield was low compared to adult performances. Results of the 2 trials were different, in relation with the galenical form of progesterone used and the frequency of drugs administration. So, these trials provide an efficient treatment scheme for induction of lactation in young heifers. [less ▲]

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