References of "Denoix, Jean-Marie"
     in
Bookmark and Share    
Full Text
Peer Reviewed
See detailÉtude des contraintes mécaniques locomotrices chez le cheval
Noble, Prisca ULg; Collin, Bernard ULg; Denoix, Jean-Marie et al

in Annales de Médecine Vétérinaire (in press)

In locomotor biomechanics, three high groups of contraints are commonly encountared : pressure, traction and torsion. In supra-maximal conditions, all of these contraints would be responsible of some ... [more ▼]

In locomotor biomechanics, three high groups of contraints are commonly encountared : pressure, traction and torsion. In supra-maximal conditions, all of these contraints would be responsible of some equine diseased locomotor systems. In order to understand better the contraints in the equine locomotor dynamics, some investigations have been carried out. Moreover, some measurement methods, based on the mechanics of Newton, have been performed. This review shows the different measurement techniques and introduces the mechanical basis that are compulsory for the understanding of the equine locomotor apparatus functioning. [less ▲]

Detailed reference viewed: 135 (21 ULg)
Full Text
Peer Reviewed
See detailQuantitative Analysis of the Relative Abundance Changes Observed in Type I, III and V Collagen after Suspensory Ligament Injuries in Horses
Shikh Al Sook, Mohamad Khir ULg; Baise, Etienne ULg; Piret, Joëlle ULg et al

in Anatomia, Histologia, Embryologia (2014, July)

Introduction: The equine suspensory ligament (SL) injuries are an important cause of lameness in horses. Quantification of the relative abundance of the constitutive collagen types is essential to ... [more ▼]

Introduction: The equine suspensory ligament (SL) injuries are an important cause of lameness in horses. Quantification of the relative abundance of the constitutive collagen types is essential to investigate the differences between normal and injured tissues. The aim of this original study was to precisely determine the different collagen ratios both in injured and normal SL. This approach is a rational step towards understanding the intimate mechanisms of SL repair and regeneration at the molecular and histological levels. Methods: Five Warmblood horses with visible signs of injury in only one forelimb SL were selected. Specimens were obtained from the central core of lesions in damaged SLs and from the corresponding regions in other healthy SLs. Collagen types I, III and V were purified by differential salt precipitation after collagen extraction with acetic acid containing pepsin and they were examined by transmission electron microscopy. The bands corresponding to types I, III, and V collagen were assessed by densitometry after SDS-PAGE. SAS software (SAS Institute 2001) was used for all statistical analyses. Results: Based on ultrastructural observations, purified fibers of types I, III, and V collagens have been identified. The relative proportions of type III and type V collagen were significantly higher in the specimens‎ from damaged tissue compared with specimens from normal tissue (P < 0.001). These changes were concomitant with significant decrease in type I collagen in the injured tissue (P < 0.001). Conclusions: Our study showed that after SL injury, the relative abundance of the different collagen types is modified. These changes are the molecular hallmark of a decrease in tissue quality and mechanical properties of the ligament. It lays down the bases of subsequent researches on the tissue regeneration that may lead to the development of new treatment strategies for damaged tissues, particularly in the equine SL injuries. [less ▲]

Detailed reference viewed: 251 (60 ULg)
Full Text
Peer Reviewed
See detailMorphometric analyses of the normal suspensory ligament in Standardbreds
Shikh Al Sook, Mohamad Khir ULg; Antoine, Nadine ULg; Piret, Joëlle ULg et al

Poster (2013, July)

The suspensory ligament (SL) is composed of connective tissue (CT) with a variable proportion of muscle (MT) and adipose tissue (AT). The aim of our study is to quantify the CT, MT and AT within the SL in ... [more ▼]

The suspensory ligament (SL) is composed of connective tissue (CT) with a variable proportion of muscle (MT) and adipose tissue (AT). The aim of our study is to quantify the CT, MT and AT within the SL in sound horses. Right limbs from 11 horses were collected. Samples from 6 levels of the SL were embedded in paraffin or in Tissue-Tek®. Most of the paraffin sections were shredded. Using the cryosection, some artefacts appeared. Cryoprotection was carried out, which produced the best results. Hematoxylin–phloxine–saffron and Hematoxylin–eosin gave a good contrast of colours between the tissues allowing the use of an image analysis programme. The percentage of MT and AT decreased significantly (P < 0.0001), whereas the percentage of CT increased significantly (P < 0.0001) with age and when descending from the proximal to the distal level of the SL. The percentage of MT was significantly higher (P < 0.0001) in females than males, while the percentage of CT was significantly higher (P < 0.0001) in males than females. The percentage of AT was significantly higher (P = 0.0278) in pelvic limbs than in thoracic limbs. These results confirm the variation in tissue composition within the SL of sound horses. [less ▲]

Detailed reference viewed: 407 (228 ULg)
Full Text
Peer Reviewed
See detailMorphometric analyses of the body and the branches of the normal third interosseous muscle (suspensory ligament) in Standardbreds
Shikh Al Sook, Mohamad Khir ULg; Antoine, Nadine ULg; Piret, Joëlle ULg et al

in Anatomia, Histologia, Embryologia (2013)

The third interosseous muscle (suspensory ligament, TIOM) is composed of connective tissue (CT) with a variable proportion of muscle (MT) and adipose tissue (AT). The aim of our study is to quantify the ... [more ▼]

The third interosseous muscle (suspensory ligament, TIOM) is composed of connective tissue (CT) with a variable proportion of muscle (MT) and adipose tissue (AT). The aim of our study is to quantify the CT, MT and AT within the body and the branches of right thoracic and pelvic limbs TIOM in sound horses to determine whether there are differences in CT, MT and AT between age, sex, limbs and levels. Right limbs from 11 sound horses were collected. Samples from 6 levels of the TIOM were embedded in paraffin or in Tissue-Tek®. Most of the paraffin sections were shredded. Using the cryosection, some artefacts appeared. Cryoprotection was carried out, which produced the best results. Hematoxylin–phloxine–saffron and Hematoxylin–eosin gave a good contrast of colours between the tissues observed allowing the use of an image analysis programme to calculate percentage of each tissue within the TIOM. The percentage of MT and AT decreased significantly (P < 0.0001), whereas the percentage of CT increased significantly (P < 0.0001) with age and when descending from the proximal to the distal level of the TIOM. The percentage of MT was significantly higher (P < 0.0001) in females than males, while the percentage of CT was significantly higher (P < 0.0001) in males than females. The percentage of AT was significantly higher (P = 0.0278) in pelvic limbs than in thoracic limbs. These results confirm the variation in tissue composition within the TIOM of sound horses. [less ▲]

Detailed reference viewed: 605 (418 ULg)
Full Text
Peer Reviewed
See detailMorphological and morphometric analyses of the suspensory ligament in Standardbreds
Shikh Al Sook, Mohamad Khir ULg; Antoine, Nadine ULg; Piret, Joëlle ULg et al

Poster (2012, October)

Ultrasound techniques allow examination of some parts of the suspensory ligament (SL) but "anomalies" are regularly observed. Their significance is not known. Few studies have described the relationship ... [more ▼]

Ultrasound techniques allow examination of some parts of the suspensory ligament (SL) but "anomalies" are regularly observed. Their significance is not known. Few studies have described the relationship between ultrasonographic appearance and the exact morphology in histological sections. The aim of this study is to develop good techniques for cutting, staining, and showing the variation in the tissue composition within the SL. The SLs from the right limbs of 11 horses were collected. Samples were taken from cross-sections at six levels of the SL and they were embedded in paraffin or in Tissue-Tek®. Most of the paraffin sections were shredded. By using the cryosection technique, some freezing artifacts (holes) appeared. Therefore, a technique of freezing with cryoprotection was carried out, which produced the best results. Hematoxylin-phloxine-saffron gives a good contrast of colors between the tissues observed allowing the use of an image analysis program. The percentage of each tissue within the SL for each section and for six levels of the ligament was calculated. Results were analyzed by SAS software. The muscle tissue (PMT) and adipose tissue (PAT) decreased significantly (p < 0.0001), whereas the connective tissue (PCT) increased significantly (p < 0.0001) with age and when descending from the proximal to the distal level of the SL. The PMT was significantly higher (p < 0.0001) in females than males, while the PCT was significantly higher (p < 0.0001) in males than females. The PAT was significantly higher (p = 0.0278) in hindlimbs than in forelimbs. [less ▲]

Detailed reference viewed: 609 (437 ULg)
Full Text
Peer Reviewed
See detailMorphology of the suspensory ligament (interosseous muscle III) of the horse
Shikh Al Sook, Mohamad Khir ULg; Espinosa, Jennifer; Piret, Joëlle ULg et al

Poster (2011)

Introduction: The injuries of the suspensory ligament (SL) are important causes of lameness and financial losses in the equine industry. Ultrasound examination permitted to visualize some parts of the SL ... [more ▼]

Introduction: The injuries of the suspensory ligament (SL) are important causes of lameness and financial losses in the equine industry. Ultrasound examination permitted to visualize some parts of the SL. The significance of “abnormal” findings is however not sufficiently known. Until now, few studies described the relationship between the ultrasonographic appearance and the exact morphology in histological sections. The aim of this study is to develop good techniques for cutting and staining the SL and to improve knowledge about the normal morphology of the SL. Methods: In this study, the SL of eight <sound> horses were collected. The body of the SL was divided in 3 thirds and sampling was realised within each third and between the thirds. The samples were embedded in paraffin or in Tissue-Tek for cryosections. The sections were stained with hematoxylin/eosin or Masson's trichrome. For 3 SL, ultrasounds were performed before sampling. The digital tip was maintained in physiological position owing to a press. Results: Most of the paraffin sections were shredded because of the hardness of the tissue. Cryosection revealed a better preservation of tissues. Only some freezing artifacts (holes) appeared on a few sections. Muscles fibers surrounded by adipose tissue containing blood vessels were present mainly in the proximal and medium third of the SL whereas they were not found in the distal third. The remaining structure look like a tendon and was composed of collagen fibers, stained in green with the Masson's trichrome coloration. Conclusions: This study permitted to develop cutting and staining techniques for the SL and helped to map the adipose, muscular and tendinous parts within the SL. It lays down the bases of subsequent studies that will concern ultrasonographically examined digital tips of sound and pathological horses of different breeds and ages. [less ▲]

Detailed reference viewed: 314 (126 ULg)
Full Text
Peer Reviewed
See detailThe relationship between fetlock joint dorsiflexion and flexor tendon moment arms variations
Noble, Prisca ULg; Collin, Bernard ULg; Denoix, Jean-Marie et al

in Anatomia, Histologia, Embryologia (2010, July 28), 39(4), 335-336

Introduction: To calculate flexor tendon forces on the basis of inverse dynamic analysis, flexor tendon moment arms need to be determinated in the equine distal forelimb. Moment arm of deep digital flexor ... [more ▼]

Introduction: To calculate flexor tendon forces on the basis of inverse dynamic analysis, flexor tendon moment arms need to be determinated in the equine distal forelimb. Moment arm of deep digital flexor tendon (dDDF) and of superficial digital flexor tendon (dSDF) have been recognised to vary during the fetlock joint dorsiflexion, however little distal forelimb model has taken into account dSDF and dDDF variations. This study describes a method to determinate the relationship between the fetlock joint dorsiflexion and the flexor moment arms variations. Methods: Eleven forelimbs of eleven horses (~500 Kg) were collected, transversally cut below the shoulder joint, frozen and cut into a sagittal plane until above the fetlock joint. Considering the forelimb pulley model (Meershoek et al. AJVR 2001; 62 1585-1593), in the segment based coordinate system, for the fetlock joint the pulley centers of the DDF and SDF tendons were determined directly from the section. For the fetlock radius-pulley experiments, forelimbs were thawed overnight, fixed to an immobilizing support and attached to a hoist that dorsiflexed the fetlock joint. For the fetlock dorsal angle (θ) 180° to 150°, the dDDF and the dSDF were measured directly from the section. For each tendon all of these data were fitted using a polynomial regression model (OriginPro8) and the relationships between θ and the radius pulley variation (∆d) was described assuming that ∆d was zero at θ equal to 180°. Results : When the fetlock dorsiflexion increased, dDDF and dSDF decreased (∆dDDF(150)= -6.1 mm; ∆dSDF(150)= -5.2 mm) according to the following equations: ∆dDDF(θ)= -0.24923 x θ1 + 0.00139 x θ² (R²=0.89867) ; ∆dSDF(θ)= -0.21494 x θ1 + 0.0012 x θ² (R²=0.91504). Conclusions : A distal forelimb pulley model with variable dDDF and dSDF has been performed. It could become an important tool for use in inverse dynamic procedures. [less ▲]

Detailed reference viewed: 138 (8 ULg)
Full Text
Peer Reviewed
See detailCompared anatomy of the locomotor system : a website
Noble, Prisca ULg; Collin, Bernard ULg; Denoix, Jean-Marie

in Anatomia, Histologia, Embryologia (2010, July 28), 39(4), 275

Introduction: This website is a multi-dimensional approach of the locomotor system in domestic animals. The first objective of this anatomical presentation is to establish relation between systemic ... [more ▼]

Introduction: This website is a multi-dimensional approach of the locomotor system in domestic animals. The first objective of this anatomical presentation is to establish relation between systemic anatomy and topographic anatomy. The second objective is to teach a practical anatomy that must be adapted to the clinician work. Methods: From the dissection of equine, bovine and canine specimens, osteology, arthrology and myology (OAM) were photographed and filmed. All photos and movies were captioned and designed using Fireworks (Adobe CS4, USA), AVS video converter 6 (MediaTechnologies Ltd, UK) and Divx Author (Divx Inc, USA). The website was designed using Dreamweaver (Adobe CS4, USA). Results: Compared anatomy site consists on dichotomously organised web pages. Each page is transversally and vertically connected with others, and always surmounted by a navigation barr. From the navigation barr we can travel through different drop-down menus (DDM). The first DDM corresponds to the lesson menu (it sends to general OAM) ; the second and third DDMs correspond to the equine picture menu and the bovine/canine picture menu (from them we can navigate in OAM per species and per system using clickable captioned photos and movies, the functional aspects being explained). For practical reasons, equine species is used as animal reference. Indeed only anatomical differences of the other species are detailed and compared. Finally the button Index corresponds to the surgeon view (all of the body is transversally cut, the clickable anatomical structures are sent back to their specific topic web page). Conclusions: Because of the numerous photos and movies, the three-dimensional view is better understood. Because of the transversal and vertical links, the navigation, in whatever level per species or per system or per anatomical cut, strengthens the cognitive process. This website is a fabulous multi-scale tool to teach and to learn the veterinary anatomy. [less ▲]

Detailed reference viewed: 116 (7 ULg)
Full Text
Peer Reviewed
See detailA pendulum test as a tool to evaluate viscous friction parameters in the equine fetlock joint
Noble, Prisca ULg; Lumay, Geoffroy ULg; Coninx, Marc ULg et al

Poster (2010, April)

This study describes an equine fetlock joint pendulum test, and investigates the influence of post mortem time and intra-articular lipid solvent on the viscous frictional response. Fresh equine digits ... [more ▼]

This study describes an equine fetlock joint pendulum test, and investigates the influence of post mortem time and intra-articular lipid solvent on the viscous frictional response. Fresh equine digits (control group 1 (n = 6); lipid solvent group 2 (n = 6)) were mounted in a pendulum tribometer. Assuming that pendular joint damping could be modelled by a harmonic oscillator fluid damping (HOFD), damping time (t), viscous damping coefficient (c ) and friction coefficient (µ) were then followed for 5 hours under experimental conditions (400 N; 20 °C). In all experiments, pendular joint damping was found to follow an exponential decay function (R² = 0.99714), which confirms that joint damping is fluid. The evolution of t, c and µ was found to be significantly (P <0.05) different in the two groups. Indeed, t decreased and c and µ increased more rapidly and strongly in the group 2. It was concluded that pendular joint damping can be modelled by an HOFD model. The influence of post mortem time on results suggests that, ideally, joint mechanical properties should only be tested on fresh cadavers at the same post mortem time. Finally, the addition of lipid solvent was found to be responsible for upper viscous friction parameters and for a reduced damping time, which suggests that articular lubricating ability was compromised. This equine pendulum test could be used to test the efficacy of various bio-lubricant treatments. [less ▲]

Detailed reference viewed: 54 (10 ULg)
Full Text
Peer Reviewed
See detailMorphology of the suspensory ligament (interosseous muscle III) of the horse
Shikh Al Sook, Mohamad Khir ULg; Espinosa, Jennifer; Piret, Joëlle ULg et al

Poster (2010)

Introduction: The injuries of the suspensory ligament (SL) are important causes of lameness and financial losses in the equine industry. Ultrasound examination permitted to visualize some parts of the SL ... [more ▼]

Introduction: The injuries of the suspensory ligament (SL) are important causes of lameness and financial losses in the equine industry. Ultrasound examination permitted to visualize some parts of the SL. The significance of “abnormal” findings is however not sufficiently known. Until now, few studies described the relationship between the ultrasonographic appearance and the exact morphology in histological sections. The aim of this study is to develop good techniques for cutting and staining the SL and to improve knowledge about the normal morphology of the SL. Methods: In this study, the SL of eight <sound> horses were collected. The body of the SL was divided in 3 thirds and sampling was realised within each third and between the thirds. The samples were embedded in paraffin or in Tissue-Tek for cryosections. The sections were stained with hematoxylin/eosin or Masson's trichrome. For 3 SL, ultrasounds were performed before sampling. The digital tip was maintained in physiological position owing to a press. Results: Most of the paraffin sections were shredded because of the hardness of the tissue. Cryosection revealed a better preservation of tissues. Only some freezing artifacts (holes) appeared on a few sections. Muscles fibers surrounded by adipose tissue containing blood vessels were present mainly in the proximal and medium third of the SL whereas they were not found in the distal third. The remaining structure look like a tendon and was composed of collagen fibers, stained in green with the Masson's trichrome coloration. Conclusions: This study permitted to develop cutting and staining techniques for the SL and helped to map the adipose, muscular and tendinous parts within the SL. It lays down the bases of subsequent studies that will concern ultrasonographically examined digital tips of sound and pathological horses of different breeds and ages. [less ▲]

Detailed reference viewed: 194 (67 ULg)
Full Text
Peer Reviewed
See detailSubclinical diseases affecting performance in Standardbred trotters: Diagnostic methods and predictive parameters
Richard, Eric ULg; Fortier, Guillaume; Pitel, Pierre-Hugues et al

in Veterinary Journal (2010), 184(3), 282-289

The objectives of this study were to determine the prevalence of sub-clinical diseases in poorly-performing Standardbred horses, compare their physiological response to exercise with control horses, and ... [more ▼]

The objectives of this study were to determine the prevalence of sub-clinical diseases in poorly-performing Standardbred horses, compare their physiological response to exercise with control horses, and identify predictive parameters of poor-performance. Fifty horses underwent thorough clinical and ancillary examinations, including haematological and biochemical evaluation, Doppler echocardiography, standardised exercise tests (SETs) on both treadmill and racetrack, treadmill video-endoscopy and collection of respiratory fluids. Most of the poorly-performing horses exhibited many concomitant diseases. The most frequently diagnosed problems involved the lower and upper respiratory tract and the musculoskeletal system. Poor-performers had lower speeds at a blood lactate (LA) concentration of 4 mmol/L (VLA4) and a heart rate (HR) of 200 bpm (V200) on treadmill and racetrack, as well as lower values for haematological parameters, plasma angiotensin-converting enzyme and antioxidants, compared to control horses. Problems of the respiratory system were the most frequently diagnosed sub-clinical diseases affecting performance. SETs, together with some blood markers, may be useful as a non-specific diagnostic tool for early detection of diseases that may affect performance. [less ▲]

Detailed reference viewed: 104 (18 ULg)
Full Text
Peer Reviewed
See detailL'articulation synoviale : un systeme tribologique parfait
Noble, Prisca ULg; Collin, Bernard ULg; Lecomte-Beckers, Jacqueline ULg et al

in Annales de Médecine Vétérinaire (2010), 154(2), 83-93

The synovial joint, by load bearing and by minimal wear and minimal friction conferring, is considered as a perfect tribological system. During these last years, researchs in articular biomechanics have ... [more ▼]

The synovial joint, by load bearing and by minimal wear and minimal friction conferring, is considered as a perfect tribological system. During these last years, researchs in articular biomechanics have been performed to study how the joint friction coefficient, a dimensionless measure which represents the ratio between the frictional force on the normal force, was close to zero. Today, there is still no consensual explanation and the articular prosthesis partially imitates the biological mechanics. This review describes the composition and the general properties of the synovial joint components. Additionally, different theorical models, on the tribology of the articular triplex cartilage-synovial fluid-cartilage, are explained. [less ▲]

Detailed reference viewed: 106 (9 ULg)
Full Text
Peer Reviewed
See detailHeel effects on joint contact force components in the equine digit : a sensitivity analysis
Noble, Prisca ULg; Lejeune, Jean-Philippe ULg; Caudron, Isabelle ULg et al

in Equine Veterinary Journal. Supplement (2010), 42

Reasons for performing study: Whereas the effect of heel configuration on the tension of the suspensory apparatus is well documented in the literature, there are few reports of joint contact force ... [more ▼]

Reasons for performing study: Whereas the effect of heel configuration on the tension of the suspensory apparatus is well documented in the literature, there are few reports of joint contact force components in the equine distal forelimb. Objectives: To improve understanding of the effect of heel configuration on equine digit joint loading, a sensitivity analysis was performed to compare the effect of a raised heel on joint contact force components in the coffin and fetlock joints during the stance phase of the trot. Materials and methods: FourWarmblood horses were used. An inverse dynamic analysis was carried out using kinematic and kinetic data. Taking into account the tendon wrapping forces (WF) around the sesamoid bones in the calculations, the joint contact forces (CF) were estimated for the coffin and fetlock joints during the trot stance phase (4 m/s). To test the sensitivity of the results to heel configuration changes, calculations were performed repeatedly for different heel configurations (raised by 0, 6 and 12°). A one-way ANOVA with repeated measures was used to test the effect of heel configuration (at the 3 levels) (a = 0.05; P<0.05; post hoc testing: Bonferroni). Results: For heel configurations raised from 0–12°: whereas the tension of the deep digital flexor tendon decreased and the tension of the superficial digital flexor tendon increased, for the coffin joint the peakWF(1.4 +- 0.25 bwt; 1.2 +- 0.2 bwt; 0.95 +- 0.1 bwt) and the peak CF (2.45 +- 0.25 bwt; 2.2 +- 0.2 bwt; 2 +- 0.1 bwt) decreased significantly (P<0.05). For the fetlock joint, the peak WF (3.8 +- 0.7 bwt; 4.1 +- 0.3 bwt; 4.4 0.25 bwt) and the peak CF (4.35 +- 0.7 bwt; 4.7 +- 0.35 bwt; 5 +- 0.3 bwt) increased, but not significantly. Conclusion: This analysis suggests that the coffin joint loading and fetlock joint loading are strongly connected. The heel configuration may influence both coffin joint and fetlock joint contact force components. [less ▲]

Detailed reference viewed: 42 (8 ULg)
Peer Reviewed
See detailEstimation of joint contact forces in the equine digit
Noble, Prisca ULg; Collin, Bernard ULg; Denoix, Jean-Marie et al

in ISB proceedings (2009, July)

Detailed reference viewed: 109 (29 ULg)
Full Text
Peer Reviewed
See detailSyndrome d'inflammation trachéale : évaluation clinique et bactériologique
Richard, Eric ULg; Maillard, K.; Dupuis, Marie-Capucine ULg et al

in Pratique Vétérinaire Equine (2009), 41(162), 45-49

Lower airway inflammation is a frequent cause of poor-performance in hors e s .An association between inflammation and bacterial isolation in tracheal wash fluid has previously been found ; bacterial ... [more ▼]

Lower airway inflammation is a frequent cause of poor-performance in hors e s .An association between inflammation and bacterial isolation in tracheal wash fluid has previously been found ; bacterial counts were neve rtheless not defined.The aims of this study were to qualitatively and quantitatively compare tracheal bacterial flora of French Trotters with tracheal inflammation (SIT) and control horses (CTL), and evaluate association between endoscopic, cytological and microbiological evaluation of the trachea. Neutrophil percentage in tracheal wash was significantly different between groups whereas tracheal mucus accumulation was not. No significant difference was found between groups for neither percentage of respectively sterile samples or percentage pathogenic bacteria being isolated. Bacterial counts in SIT group were however 10 to 200 times higher than in the CTL group.This study suggests that isolation of pathogenic bacteria alone do not allow estimation of the level of airway inflammation; whereas severe tracheal inflammation may be present in absence of any bacteriological infection [less ▲]

Detailed reference viewed: 346 (16 ULg)
Full Text
Peer Reviewed
See detailInfluence of subclinical inflammatory airway disease on equine respiratory function evalueated by impulse oscillometry
Richard, Eric ULg; Fortier, Guillaume; Denoix, Jean-Marie et al

in Equine Veterinary Journal (2009), 41(4), 384-389

Reasons for performing study: Inflammatory airway disease (IAD) is a nonseptic condition of the lower respiratory tract. Its negative impact on respiratory function has previously <br /><br />been ... [more ▼]

Reasons for performing study: Inflammatory airway disease (IAD) is a nonseptic condition of the lower respiratory tract. Its negative impact on respiratory function has previously <br /><br />been described using either forced expiration or forced oscillations techniques. However, sedation or drug-induced bronchoconstriction were usually required. The impulse <br /><br />oscillometry system (IOS) is a noninvasive and sensitive respiratory function test validated in horses, which could be useful to evaluate IAD-affected horses without further <br /><br />procedures. <br /><br />Objectives: To determine the sensitivity of IOS in detecting alterations of the respiratory function in subclinically IAD-affected horses without inducing bronchoprovocation and to characterise their respiratory impedance according to frequency for each respiratory phase. <br /><br />Methods: Pulmonary function was evaluated at rest by IOS in 34 Standardbred trotters. According to the cytology of bronchoalveolar lavage fluid (BALF), 19 horses were defined <br /><br />as IAD-affected and 15 horses were used as control (CTL). Total respiratory resistance (Rrs) and reactance (Xrs) from 1–20 Hz as well as their inspiratory and expiratory <br /><br />components were compared between groups. <br /><br />Results: A significant increase of Rrs at the lower frequencies (R1–10 Hz) as well as a significant decrease of Xrs beyond 5 Hz (X5–20 Hz) was observed in IAD compared to CTL horses. IOS-data was also significantly different between inspiration and expiration in IAD-affected horses. In the whole population, both BALF eosinophil and mast cell counts were <br /><br />significantly correlated with IOS measurements. <br /><br />Conclusions: Functional respiratory impairment may be measured, even in the absence of clinical signs of disease. In IAD-affected horses, the different parameters of respiratory <br /><br />function (Rrs or Xrs) may vary depending on the inflammatory cell profiles represented in BALF. <br /><br />Potential relevance: Impulse oscillometry could be used in a routine clinical setting as a noninvasive method for early detection of subclinical respiratory disease and of the results <br /><br />of treatment in horses. [less ▲]

Detailed reference viewed: 176 (61 ULg)
Full Text
Peer Reviewed
See detailExamen échographique transcunéen post mortem de l'appareil podotrochléaire équin à l'aide des sondes linéaires et sectorielles
Schneider, Nicole ULg; Busoni, Valeria ULg; de la Rebière de Pouyade, Geoffroy ULg et al

in Annales de Médecine Vétérinaire (2008), 152

Ultrasound has become more common in the investigation of the podotrochlear apparatus in the horse and the use of more sensible scanheads improved the quality of the images. This study shows six examples ... [more ▼]

Ultrasound has become more common in the investigation of the podotrochlear apparatus in the horse and the use of more sensible scanheads improved the quality of the images. This study shows six examples of the ultrasound anatomy of the distal podotrochlear apparatus in the transcuneal view performed with three different scanheads. The presented transcuneal scans of the podotrochlear apparatus of an 18 months old warmblood foal have been established post mortem on isolated feet. Modus of registration was real-timemotion, linear- and sectorscanheads working at 7.5 (linear) and 7.5 (microconvex) and 3.5 (convex) MHz were used. The echoanatomic structures of the soft tissue and bony surfaces in the distal podotrochlear region are compared by six images. The combination of the different scanheads permits an improved complete visualisation in this area. Therefore these ultrasound pictures show that ultrasonography is an useful complementary investigation method that enables to precise the diagnosis [less ▲]

Detailed reference viewed: 85 (14 ULg)
Full Text
Peer Reviewed
See detailTranscuneal ultrasonographic findings in the podotrochlear apparatus: Comparison with postmortem in 14 equine digits
Busoni, Valeria ULg; Lahaye, Benoit; Denoix, Jean-Marie

in Journal of Equine Veterinary Science (2006), 26(3), 113-119

The objective was to describe the ultrasonographic features of the lesions of the podotrochlear apparatus identified when the foot is imaged through the frog in equine cadaver digits and to compare the ... [more ▼]

The objective was to describe the ultrasonographic features of the lesions of the podotrochlear apparatus identified when the foot is imaged through the frog in equine cadaver digits and to compare the ultrasonographic graphic changes with macroscopic findings. Fourteen cadaver feet with abnormalities of the podotrochlear apparatus detected by transcuneal ultrasonography were used for the study. The palmarodistal portion of the podotrochlear apparatus was studied and imaged using the transcuneal approach. The detectable ultrasonographic findings suggesting pathology of the podotrochlear apparatus were described. Ultrasonographic and gross findings in the podotrochlear apparatus were compared to interpret the transcuneal ultrasonographic changes. [less ▲]

Detailed reference viewed: 36 (3 ULg)