References of "Deleuze, Stefan"
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See detailCase report: Ovarian fibroma in a mare – Hormonal considerations
Ponthier, Jérôme ULg; Salciccia, Alexandra ULg; Parrilla Hernandez, Sonia ULg et al

in Reproduction in Domestic Animals (2016, October 27), 51(supplement 2), 132

This report shows that, as in humans, AMH as well as steroids productions are low in case of ovarian fibroma, thus preserving normal cyclicity.

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See detailCan serum progesterone be used to decide altrenogest supplementation discontinuation in problem pregnancies in mares?
Parrilla Hernandez, Sonia ULg; Delahaut, Philippe; Ponthier, Jérôme ULg et al

Poster (2016, June)

Use of oral altrenogest as exogenous progesterone (P4) in mares in an effort to maintain high-risk pregnancies is a widespread practice. Treatment is sometimes initiated as early as 2 days after ovulation ... [more ▼]

Use of oral altrenogest as exogenous progesterone (P4) in mares in an effort to maintain high-risk pregnancies is a widespread practice. Treatment is sometimes initiated as early as 2 days after ovulation. Timing when it can safely be stopped is frequently uncertain and therefore, supplementation is often continued until 100-120 days, when the placental secretion of progestagens has taken over. The aim of this study was to determine if altrenogest interfered with progesterone assay and if timing of altrenogest administration discontinuation could be based on the blood concentration of P4. Data were obtained from 7 healthy standard breed mares in seasonal anoestrus and in dioestrus. Mares were considered anoestrus when no follicles larger than 15 mm in diameter or corpus luteum (CL) were found at 2 consequent ultrasound examinations 7 days apart. They were confirmed dioestrus based on an observed ovulation and the presence of a CL 2 days after. Mares were treated with 0.044 mg/kg of altrenogest (Regumate®, Intervet, Boxmeer, Netherlands) orally once per day during 12 days beginning at any time for the anestrus group and at day 2 after ovulation for the dioestrus group. Blood was collected at day 0, 6, 12, 18 right before altrenogest administration and the serum was stored at -20°C until analysis. P4 concentration was obtained by radioimmunoassay and altrenogest by liquid chromatography-mass spectrometry. Differences in P4 and in altrenogest concentrations between different sampling days were determined by Friedman non parametric test. Concentrations of altrenogest before treatment were almost not detectable (negative control). At day 6, they were significantly higher (p<0.05), and were back to basal, almost not detectable values at day 18, in both groups. P4 concentration was basal with values < 0.4 ng/ml all through the experiment in the anoestrus group. In the dioestrus group, P4 concentration was variable from one mare to another, but it was significantly higher in mid-dioestrus (day 6) as compared to the time of the impending next oestrus (day12). No cross-reaction between P4 and altrenogest assays was observed as illustrated by the fact that concentration of P4 during anoestrus remained basal with or without supplementation. Despite the treatment, the variation of P4 over time was as expected in both groups. Altrenogest was no longer detectable 6 days after the last administration. Further studies with larger numbers and sampling in dioestrus to compare P4 levels in treated and untreated mares to investigate the potential effect of altrenogest on endogenous P4 production by the CL are needed. However, our experiment shows that P4 levels assayed by RIA can be trusted to evaluate if the secondary CL’s have become functional and can thus maintain the pregnancy while oral altrenogest supplementation is discontinued. [less ▲]

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See detailBiotechnolgia w rozrodzie koni
Deleuze, Stefan ULg; Gajewski, Zdzislaw; Faundez, Ricardo et al

in Rozrod koni, Wybrane aspekty IV Monograph (2016)

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See detailExploration fonctionnelle du système reproducteur mâle.
Deleuze, Stefan ULg; Ponthier, Jérôme ULg

in Bardiès, Jacques (Ed.) Manuel pratique de thérapeutique équine (2014)

Les tests hormonaux sont utiles pour diagnostiquer si il existe du tissu testiculaire dans l’animal présenté. Ils interviennent donc dans le diagnostic différentiel du cheval présentant un caractère ... [more ▼]

Les tests hormonaux sont utiles pour diagnostiquer si il existe du tissu testiculaire dans l’animal présenté. Ils interviennent donc dans le diagnostic différentiel du cheval présentant un caractère d’étalon mais qui n’a pas de testicule apparent. Ce diagnostic différentiel comprend : - le cheval hongre ; - les cas de cryptorchidies bilatérales ; - le cheval castré unilatéralement chez lequel le testicule cryptorchide a été laissé en place. Le but de ces tests hormonaux est de déterminer si il existe encore des sécrétions de molécules spécifiques du tissu testiculaire. [less ▲]

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See detailA case of true hermaphrodism in a horse
Parrilla-Hernadez, Sonia; de la Rebière de Pouyade, Geoffroy ULg; Grulke, Sigrid ULg et al

in Reproduction in Domestic Animals (2014, September), 49(suppl 3), 50

True hermaphroditism in horses, is a complex and poorly under- stood disorder of sexual development characterized by the presence of both male and female gonads as separate organs or in a combined ... [more ▼]

True hermaphroditism in horses, is a complex and poorly under- stood disorder of sexual development characterized by the presence of both male and female gonads as separate organs or in a combined structure called ovotestis. A 3-year-old Spanish nullipa- rous mare with a history of stallion behaviour and an abnormal reproductive exam was presented at the Equine Clinic of the University of Li ege. Externally, a small vulva with an enlarged clitoris was observed. Transrectal ultrasonography showed a thin (<1 cm) uterus, leading to a small heterogeneous structure in the normal place of the left ovary. No structure could be identified in the presumed location of the right ovary. Speculum exam was compatible with a vaginal agenesis, as the cervix opened craniad the urethral meatus. Oestradiol, progesterone and testosterone concen- trations were compatible with a stallion’s endocrinology. Gonads were laparoscopically found in the usual location of the ovaries in both flanks and excised. Histopathology of both gonads showed atrophic testicular tissue with hyperplastic Leydig cells. The left gonad also contained ovarian tissue with some scarce primordial follicles. Clitoral enlargement is the first symptom most commonly identified in animals with true hermaphroditism and can be explained by the production of testosterone by the gonads, however, the aetiology of the vaginal agenesis is unknown. True hermaph- roditism in horses is generally related to 64XX syndrome with or without SRY gene translocation (impending karyotyping analysis). After 5 days the mare was discharged from the clinic, and in absence of testosterone the stallion behaviour disappeared in a couple of months. [less ▲]

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See detailGlucose use and lactate production by equine fresh semen in human and equine extender
Ponthier, Jérôme ULg; De Tullio, Pascal ULg; Parrilla-Hernandez, Sonia et al

in Reproduction in Domestic Animals (2014, September), 49(suppl 3), 13

This study shows that this human semen extender doesn’t support equine semen preservation. Sperm cells’ glucose consumption and lactate production seem to be negligible, as these parameters were not ... [more ▼]

This study shows that this human semen extender doesn’t support equine semen preservation. Sperm cells’ glucose consumption and lactate production seem to be negligible, as these parameters were not affected by sperm concentrations in our study. Our results suggest that spermatozoa are able to cleave complex carbohydrates as glucose concentration in INRA96 increased over time. [less ▲]

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See detailHigh concentrations of Myeloperoxidase in the equine uterus as an indicator of endometritis
Parrilla Hernandez, Sonia ULg; Ponthier, Jérôme ULg; Franck, Thierry ULg et al

in Theriogenology (2014), 81(7), 936-940

Intra-luminal fluid and excessive abnormal hyper-edema are regularly used for the diagnosis of endometritis in the mare, which is routinely confirmed by the presence of neutrophils on endometrial smears ... [more ▼]

Intra-luminal fluid and excessive abnormal hyper-edema are regularly used for the diagnosis of endometritis in the mare, which is routinely confirmed by the presence of neutrophils on endometrial smears. Studies show a relation between neutrophils and myeloperoxidase (MPO), an enzyme contained in and released by neutrophils during degranulation or after cell lysis. This enzyme has been found in many fluids and tissues and associated with different inflammatory pathologies in the horse. The aims of this study were to assess the presence and concentration of MPO in the equine uterus, and to investigate its relation with neutrophils, and other clinical signs of endometritis. Mares (n=51) were evaluated for presence of intra-luminal fluid and excessive endometrial edema before breeding, and a small volume lavage and cytology samples were obtained. From 69 cycles, supernatant of the uterine flushes was analysed with a specific equine MPO ELISA assay to measure MPO concentration. Cytology samples were used for the diagnosis of endometritis. MPO was present in the uterus of all estrus mares in highly variable concentrations. MPO concentrations were significantly (p<0.05) higher in samples with positive cytologies and in presence of intra-luminal fluid. Occasionally, some samples with negative cytologies showed high MPO concentration, but the opposite was never observed. Cycles presenting hyper-edema weren’t associated to high concentration of MPO, intra-luminal fluid or positive cytology making it a poor diagnostic tool of endometritis. [less ▲]

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See detailSperm Motility and Lactate production at different sperm concentrations
Ponthier, Jérôme ULg; De Tullio, Pascal ULg; Blommaert, Didier ULg et al

in Journal of Equine Veterinary Science (2014, January), 34(1), 75-76

Lactate production is associated with total spermatozoa concentration. It negatively affects preservation of total and progressive motility, showing an effect of by-products of anaerobic metabolism on ... [more ▼]

Lactate production is associated with total spermatozoa concentration. It negatively affects preservation of total and progressive motility, showing an effect of by-products of anaerobic metabolism on long-term storage. Moreover, our data show that non-progressive motile spermatozoa are highly associated to lactate concentration, and thus, anaerobic glycolysis. More studies are required to determine relative contributions of aerobiosis and anaerobiosis to spermatozoa motility under different storage conditions. [less ▲]

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See detailCongélation de sperme dans l'espèce équine: état des lieux et perspectives
Ponthier, Jérôme ULg; van den Berghe, Femke; Parrilla Hernandez, Sonia ULg et al

in Annales de Médecine Vétérinaire (2014), 159(1), 56-71

Le but de cette revue est de décrire les techniques de congélation de la semence équine. Un rappel résumera la formation du spermatozoïde dans le testicule, son anatomie et sa physiologie en insistant sur ... [more ▼]

Le but de cette revue est de décrire les techniques de congélation de la semence équine. Un rappel résumera la formation du spermatozoïde dans le testicule, son anatomie et sa physiologie en insistant sur les structures lésées pendant le processus de congélation et décongélation. Les techniques de congélation du sperme seront ensuite résumées dans différentes espèces et comparées avec celles utilisées pour la semence équine. Les critères actuels de qualité seront définis pour le sperme avant et après congélation en insistant sur la mobilité totale, progressive et les différentes définitions de ces concepts dans la littérature. Les autres méthodes d’analyse comme la cytométrie de flux seront présentées ainsi que leurs intérêts cliniques. Les prochains développements et progrès dans le domaine de la congélation seront enfin envisagés : la prédiction de la qualité du sperme congelé à partir du sperme frais, l’amélioration des dilueurs et la lutte contre les formes activées de l’oxygène. [less ▲]

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See detailConcentration, Activity and Biochemical Characterization of Myeloperoxidase in Fresh and Post-thaw equine semen and their Implication on Freezability
Ponthier, Jérôme ULg; Franck, Thierry ULg; Parrilla Hernandez, Sonia ULg et al

in Reproduction in Domestic Animals (2014)

Myeloperoxidase (MPO) is a pro-oxidant enzyme associated with decreased motility in thawed equine semen. This study aimed to describe MPO concentration, activity and subunits in raw and thawed semen and ... [more ▼]

Myeloperoxidase (MPO) is a pro-oxidant enzyme associated with decreased motility in thawed equine semen. This study aimed to describe MPO concentration, activity and subunits in raw and thawed semen and to correlate these data with motilities in raw and thawed semen. Semen samples from five stallions were collected four times. Motilities were assessed in raw and thawed semen. MPO assays were performed in raw seminal plasma, raw sperm-rich pellet and thawed semen. Total and active MPO concentrations were, respectively, assayed by enzyme-linked immunosorbent assay and specific immunological extraction followed by enzymatic detection. MPO subunits present in semen were characterized by Western 3 blot. Purified active MPO was added in PBS and freezing extender to control its activity during freezing procedure. Differences between medians were determined using Kruskal– Wallis test, and correlations were determined using Spearman’s test for nonparametric data. Active MPO concentration was low in seminal plasma and thawed semen, but high in pellet (p = 0.0058), as the opposite relation was observed for total MPO concentration (p < 0.0001). In seminal plasma and post- thaw semen, inactive 86-kDa MPO precursor was mainly observed. Purified MPO activity was decreased in the extender (p = 0.0286). MPO activity in pellet was highly correlated with thawed progressive motility (r = 􏰑0.5576, p = 0.0086). Inac- tive MPO precursor and unknown low molecular weight inactive MPO precursor subunits explain low MPO activity in semen. Major MPO activity was observed in pellet, and post- thaw loss of activity is partially explained by MPO inactiva- tion in extender. Thawed semen motility was negatively correlated with MPO activity in pellet, becoming a potential freezability predictor. [less ▲]

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See detailCT findings of an intra-abdominal metastatic sertoli cell tumor in a dog
Taylor, Olivia; Hamaide, Annick ULg; Mercier, Elise ULg et al

Poster (2013, December 01)

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See detailEffect of non-sperm cells removal with single layer colloidal centrifugation on myeloperoxidase concentration in post-thaw equine semen
Ponthier, Jérôme ULg; Teague, Sheila; Franck, Thierry ULg et al

in Theriogenology (2013), 80(9), 1082-1087

Myeloperoxidase (MPO) is a pro-oxidant enzyme contained in, and released by, neutrophils during degranulation or after lysis. Post-thaw semen contains MPO and its concentration is associated with ... [more ▼]

Myeloperoxidase (MPO) is a pro-oxidant enzyme contained in, and released by, neutrophils during degranulation or after lysis. Post-thaw semen contains MPO and its concentration is associated with decreased sperm motility. Recently, MPO concentration in post-thaw semen was shown to be associated with presence of non-sperm cells. The aim of this study was to describe the effect of centrifugal fractionation of semen prior to cryopreservation on post-thaw concentrations of non-sperm cells and MPO. The experimental design consisted in freezing semen with or without previous centrifugation through two concentrations of single layer colloid media. Non-sperm cells and MPO concentrations were assessed in pellet and upper layer at each step of the procedure and MPO was detected in cells by immunocytochemistry. Single layer colloid centrifugation decreased non-sperm cells and MPO concentrations in post-thaw semen. The MPO concentration was correlated with concentration of non-sperm cells in the upper layer of the supernatant. In post-thaw semen, with or without previous single layer colloid centrifugation, MPO concentration was correlated with concentration of non-sperm cells. Overall, neutrophils were rarely observed and non-sperm cells were mainly epithelial cells or cellular debris, as demonstrated by MPO immunocytochemistry. Following single layer colloid centrifugation, MPO concentration was decreased, and correlations observed in different samples of the experiments revealed an association between concentrations of MPO and non-sperm cells. At all steps of the semen processing and cryopreservation, MPO immunostaining was clearly identified only on non-sperm cells. Our results demonstrate that non-sperm cells present in fresh semen release MPO during freezing. [less ▲]

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See detailVitrification of immature equine oocytes
Gatez, Carine ULg; Ectors, Fabien ULg; Farnir, Frédéric ULg et al

Poster (2013, October 11)

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See detailPyovagina caused by pyometra and a persistent hymen in a 9-year-old bitch
Van Den Berghe, Femke ULg; Ponthier, Jérôme ULg; Parrilla Hernandez, Sonia ULg et al

in Reproduction in Domestic Animals (2013, September), 48(suppl1), 114

Pyovagina with a persistent hymen is very uncommon in a 9-year-old intact bitch. Blood discharge during prooestrus phases of previous cycles and the recent purulent vulvar discharge indicate an -at least ... [more ▼]

Pyovagina with a persistent hymen is very uncommon in a 9-year-old intact bitch. Blood discharge during prooestrus phases of previous cycles and the recent purulent vulvar discharge indicate an -at least partially- perforated hymen. For an unknown cause the hymen must have re-sealed after uterine contamination explaining a pyovagina that was bulging in the perineal area [less ▲]

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See detailInfluence of concentration on equine fresh semen conservation
Ponthier, Jérôme ULg; Bloomaert, Didier; Parrilla Hernandez, Sonia ULg et al

in Reproduction in Domestic Animals (2013, September), 48(suppl1), 85

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See detailA case of Longitudinal Vaginal Septum in a mare
Parrilla Hernandez, Sonia ULg; Ponthier, Jérôme ULg; Deleuze, Stefan ULg

in Reproduction in Domestic Animals (2013, September), 48(suppl1), 112-113

LVS occurs when there is failure in the fusion of müllerian ducts or in the regression of the vaginal septum. Primary or secondary cause of infertility, increases the risk of dystocia or alter sport ... [more ▼]

LVS occurs when there is failure in the fusion of müllerian ducts or in the regression of the vaginal septum. Primary or secondary cause of infertility, increases the risk of dystocia or alter sport performance. It is most likely under-diagnosed as it is often asymptomatic and an incidental finding. [less ▲]

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See detailMyeloperoxidase activity decreases in equine semen freezing extenders
Ponthier, Jérôme ULg; Franck, Thierry ULg; Niesten, Ariane ULg et al

in Amir, Arav (Ed.) Proceedings of the 3rd Cryo Congress (2013, March 23)

Myeloperoxidase (MPO) is a pro-oxidant enzyme contained in and released by neutrophils, and associated with decreased post-thaw motility of equine semen. This study aimed to compare MPO activity in pure ... [more ▼]

Myeloperoxidase (MPO) is a pro-oxidant enzyme contained in and released by neutrophils, and associated with decreased post-thaw motility of equine semen. This study aimed to compare MPO activity in pure equine freezing extender, raw and post-thaw semen. Active MPO Concentration (AMC) was measured with Specific Immunologic Extraction Followed by Enzymatic Detection in 20 ejaculates. Raw semen intra cellular AMC was determined in the supernatant after membrane lysis, each pellet containing 100x106 spermatozoa. AMC was also assayed in supernatants of semen frozen following a conventional method using INRA FreezeTM (IMV, France). Effect of freezing procedure on AMC was tested by experimentally adding 500ng of purified active MPO (Calbiochem, Germany) in 4 samples either with 5ml of PBS or INRA FreezeTM before assay. AMC was higher in sperm-rich pellet (0.306ng/ml) than in post-thaw semen (0.002ng/ml) (p=0.058). After experimental MPO addition, no activity variation was observed during the freezing procedure (after dilution, 1, 2 hours of cooling and post-thawing) within the same medium. Purified MPO activity was decreased in INRA FreezeTM when compared to PBS at all timings of sampling (p=0.0286). When all samples were pooled, remaining activity in INRA FreezeTM was 23.93±13.13%. MPO fixation on large proteins contained in the extender experimentally reduces AMC, as previously observed in plasma. However, AMC decrease observed during semen freezing is more important than after experimental addition. That could be explained by a MPO interaction with seminal plasma, a partial MPO release or a MPO inactivation during equine semen freezing. [less ▲]

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