References of "Deleuze, Stefan"
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See detailHigh concentrations of Myeloperoxidase in the equine uterus as an indicator of endometritis
Parrilla Hernandez, Sonia ULg; Ponthier, Jérôme ULg; Franck, Thierry ULg et al

in Theriogenology (in press)

Intra-luminal fluid and excessive abnormal hyper-edema are regularly used for the diagnosis of endometritis in the mare, which is routinely confirmed by the presence of neutrophils on endometrial smears ... [more ▼]

Intra-luminal fluid and excessive abnormal hyper-edema are regularly used for the diagnosis of endometritis in the mare, which is routinely confirmed by the presence of neutrophils on endometrial smears. Studies show a relation between neutrophils and myeloperoxidase (MPO), an enzyme contained in and released by neutrophils during degranulation or after cell lysis. This enzyme has been found in many fluids and tissues and associated with different inflammatory pathologies in the horse. The aims of this study were to assess the presence and concentration of MPO in the equine uterus, and to investigate its relation with neutrophils, and other clinical signs of endometritis. Mares (n=51) were evaluated for presence of intra-luminal fluid and excessive endometrial edema before breeding, and a small volume lavage and cytology samples were obtained. From 69 cycles, supernatant of the uterine flushes was analysed with a specific equine MPO ELISA assay to measure MPO concentration. Cytology samples were used for the diagnosis of endometritis. MPO was present in the uterus of all estrus mares in highly variable concentrations. MPO concentrations were significantly (p<0.05) higher in samples with positive cytologies and in presence of intra-luminal fluid. Occasionally, some samples with negative cytologies showed high MPO concentration, but the opposite was never observed. Cycles presenting hyper-edema weren’t associated to high concentration of MPO, intra-luminal fluid or positive cytology making it a poor diagnostic tool of endometritis. [less ▲]

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See detailConcentration, Activity and Biochemical Characterization of Myeloperoxidase in Fresh and Post-thaw equine semen and their Implication on Freezability
Ponthier, Jérôme ULg; Franck, Thierry ULg; Parrilla Hernandez, Sonia ULg et al

in Reproduction in Domestic Animals (in press)

Myeloperoxidase (MPO) is a pro-oxidant enzyme associated with decreased motility in thawed equine semen. This study aimed to describe MPO concentration, activity and subunits in raw and thawed semen and ... [more ▼]

Myeloperoxidase (MPO) is a pro-oxidant enzyme associated with decreased motility in thawed equine semen. This study aimed to describe MPO concentration, activity and subunits in raw and thawed semen and to correlate these data with motilities in raw and thawed semen. Semen samples from five stallions were collected four times. Motilities were assessed in raw and thawed semen. MPO assays were performed in raw seminal plasma, raw sperm-rich pellet and thawed semen. Total and active MPO concentrations were, respectively, assayed by enzyme-linked immunosorbent assay and specific immunological extraction followed by enzymatic detection. MPO subunits present in semen were characterized by Western 3 blot. Purified active MPO was added in PBS and freezing extender to control its activity during freezing procedure. Differences between medians were determined using Kruskal– Wallis test, and correlations were determined using Spearman’s test for nonparametric data. Active MPO concentration was low in seminal plasma and thawed semen, but high in pellet (p = 0.0058), as the opposite relation was observed for total MPO concentration (p < 0.0001). In seminal plasma and post- thaw semen, inactive 86-kDa MPO precursor was mainly observed. Purified MPO activity was decreased in the extender (p = 0.0286). MPO activity in pellet was highly correlated with thawed progressive motility (r = 􏰑0.5576, p = 0.0086). Inac- tive MPO precursor and unknown low molecular weight inactive MPO precursor subunits explain low MPO activity in semen. Major MPO activity was observed in pellet, and post- thaw loss of activity is partially explained by MPO inactiva- tion in extender. Thawed semen motility was negatively correlated with MPO activity in pellet, becoming a potential freezability predictor. [less ▲]

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See detailSperm Motility and Lactate production at different sperm concentrations
Ponthier, Jérôme ULg; De Tullio, Pascal ULg; Blommaert, Didier ULg et al

in Journal of Equine Veterinary Science (2014, January), 34(1), 75-76

Lactate production is associated with total spermatozoa concentration. It negatively affects preservation of total and progressive motility, showing an effect of by-products of anaerobic metabolism on ... [more ▼]

Lactate production is associated with total spermatozoa concentration. It negatively affects preservation of total and progressive motility, showing an effect of by-products of anaerobic metabolism on long-term storage. Moreover, our data show that non-progressive motile spermatozoa are highly associated to lactate concentration, and thus, anaerobic glycolysis. More studies are required to determine relative contributions of aerobiosis and anaerobiosis to spermatozoa motility under different storage conditions. [less ▲]

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See detailCT findings of an intra-abdominal metastatic sertoli cell tumor in a dog
Taylor, Olivia; Hamaide, Annick ULg; Mercier, Elise ULg et al

Poster (2013, December 01)

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See detailEffect of non-sperm cells removal with single layer colloidal centrifugation on myeloperoxidase concentration in post-thaw equine semen
Ponthier, Jérôme ULg; Teague, Sheila; Franck, Thierry ULg et al

in Theriogenology (2013), 80(9), 1082-1087

Myeloperoxidase (MPO) is a pro-oxidant enzyme contained in, and released by, neutrophils during degranulation or after lysis. Post-thaw semen contains MPO and its concentration is associated with ... [more ▼]

Myeloperoxidase (MPO) is a pro-oxidant enzyme contained in, and released by, neutrophils during degranulation or after lysis. Post-thaw semen contains MPO and its concentration is associated with decreased sperm motility. Recently, MPO concentration in post-thaw semen was shown to be associated with presence of non-sperm cells. The aim of this study was to describe the effect of centrifugal fractionation of semen prior to cryopreservation on post-thaw concentrations of non-sperm cells and MPO. The experimental design consisted in freezing semen with or without previous centrifugation through two concentrations of single layer colloid media. Non-sperm cells and MPO concentrations were assessed in pellet and upper layer at each step of the procedure and MPO was detected in cells by immunocytochemistry. Single layer colloid centrifugation decreased non-sperm cells and MPO concentrations in post-thaw semen. The MPO concentration was correlated with concentration of non-sperm cells in the upper layer of the supernatant. In post-thaw semen, with or without previous single layer colloid centrifugation, MPO concentration was correlated with concentration of non-sperm cells. Overall, neutrophils were rarely observed and non-sperm cells were mainly epithelial cells or cellular debris, as demonstrated by MPO immunocytochemistry. Following single layer colloid centrifugation, MPO concentration was decreased, and correlations observed in different samples of the experiments revealed an association between concentrations of MPO and non-sperm cells. At all steps of the semen processing and cryopreservation, MPO immunostaining was clearly identified only on non-sperm cells. Our results demonstrate that non-sperm cells present in fresh semen release MPO during freezing. [less ▲]

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See detailVitrification of immature equine oocytes
Gatez, Carine ULg; Ectors, Fabien ULg; Farnir, Frédéric ULg et al

Poster (2013, October 11)

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See detailPyovagina caused by pyometra and a persistent hymen in a 9-year-old bitch
Van Den Berghe, Femke ULg; Ponthier, Jérôme ULg; Parrilla Hernandez, Sonia ULg et al

in Reproduction in Domestic Animals (2013, September), 48(suppl1), 114

Pyovagina with a persistent hymen is very uncommon in a 9-year-old intact bitch. Blood discharge during prooestrus phases of previous cycles and the recent purulent vulvar discharge indicate an -at least ... [more ▼]

Pyovagina with a persistent hymen is very uncommon in a 9-year-old intact bitch. Blood discharge during prooestrus phases of previous cycles and the recent purulent vulvar discharge indicate an -at least partially- perforated hymen. For an unknown cause the hymen must have re-sealed after uterine contamination explaining a pyovagina that was bulging in the perineal area [less ▲]

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See detailInfluence of concentration on equine fresh semen conservation
Ponthier, Jérôme ULg; Bloomaert, Didier; Parrilla Hernandez, Sonia ULg et al

in Reproduction in Domestic Animals (2013, September), 48(suppl1), 85

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See detailA case of Longitudinal Vaginal Septum in a mare
Parrilla Hernandez, Sonia ULg; Ponthier, Jérôme ULg; Deleuze, Stefan ULg

in Reproduction in Domestic Animals (2013, September), 48(suppl1), 112-113

LVS occurs when there is failure in the fusion of müllerian ducts or in the regression of the vaginal septum. Primary or secondary cause of infertility, increases the risk of dystocia or alter sport ... [more ▼]

LVS occurs when there is failure in the fusion of müllerian ducts or in the regression of the vaginal septum. Primary or secondary cause of infertility, increases the risk of dystocia or alter sport performance. It is most likely under-diagnosed as it is often asymptomatic and an incidental finding. [less ▲]

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See detailMyeloperoxidase activity decreases in equine semen freezing extenders
Ponthier, Jérôme ULg; Franck, Thierry ULg; Niesten, Ariane ULg et al

in Amir, Arav (Ed.) Proceedings of the 3rd Cryo Congress (2013, March 23)

Myeloperoxidase (MPO) is a pro-oxidant enzyme contained in and released by neutrophils, and associated with decreased post-thaw motility of equine semen. This study aimed to compare MPO activity in pure ... [more ▼]

Myeloperoxidase (MPO) is a pro-oxidant enzyme contained in and released by neutrophils, and associated with decreased post-thaw motility of equine semen. This study aimed to compare MPO activity in pure equine freezing extender, raw and post-thaw semen. Active MPO Concentration (AMC) was measured with Specific Immunologic Extraction Followed by Enzymatic Detection in 20 ejaculates. Raw semen intra cellular AMC was determined in the supernatant after membrane lysis, each pellet containing 100x106 spermatozoa. AMC was also assayed in supernatants of semen frozen following a conventional method using INRA FreezeTM (IMV, France). Effect of freezing procedure on AMC was tested by experimentally adding 500ng of purified active MPO (Calbiochem, Germany) in 4 samples either with 5ml of PBS or INRA FreezeTM before assay. AMC was higher in sperm-rich pellet (0.306ng/ml) than in post-thaw semen (0.002ng/ml) (p=0.058). After experimental MPO addition, no activity variation was observed during the freezing procedure (after dilution, 1, 2 hours of cooling and post-thawing) within the same medium. Purified MPO activity was decreased in INRA FreezeTM when compared to PBS at all timings of sampling (p=0.0286). When all samples were pooled, remaining activity in INRA FreezeTM was 23.93±13.13%. MPO fixation on large proteins contained in the extender experimentally reduces AMC, as previously observed in plasma. However, AMC decrease observed during semen freezing is more important than after experimental addition. That could be explained by a MPO interaction with seminal plasma, a partial MPO release or a MPO inactivation during equine semen freezing. [less ▲]

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See detailRelationship between echocardiographic measurements and body size in horses.
Al Haidar, Ali; Leroux, Aurélia ULg; Borde, Laura ULg et al

in Journal of Equine Veterinary Science (2013), 33(2), 107-114

The objectives of this work were to evaluate the optimal body size variable, and to determine mathematical model that best fits echocardiographic measurements to body size in the equine species. 150 ... [more ▼]

The objectives of this work were to evaluate the optimal body size variable, and to determine mathematical model that best fits echocardiographic measurements to body size in the equine species. 150 horses of various breeds, aged 1 week to 17 years old, body weight (BW) 38 to 890 kg, and free from cardiac disease were used in this study. Based on their age (i.e. younger or older than 2 years), animals were separated into 2 groups. In each horse, the body weight (BW), height at withers (HT), thoracic circumference (TC) and the body length (BL) were measured, the body surface area (BSA) was calculated, and a standard two-dimensional and M-mode echocardiography was performed allowing classic cardiac measurements to be taken. Correlations between each echocardiographic measurement and each of the anthropometric variables were assessed via a Pearson’s product-moment analysis and using linear, logarithmic, and power regression analysis in all animals and within the 2 groups. The dimensional, but not the functional, echocardiographic measurements showed a close correlation with all body size variables, especially during growth. The relationship between cardiac measurements and body size was best described using power regression models with TC as the anthropometric variable, but the relationship was also very accurately described using BW, BSA, or BL. In the equine species, dimensional echocardiographic reference values should be established using power regression equations on TC. Such a correction for body size could increase the diagnostic value of echocardiography in equine cardiology. [less ▲]

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See detailEffect of breed, sex, age and body weight on Echocardiographic Measurements in the Equine Species
Al Haidar, Ali; Farnir, Frédéric ULg; Deleuze, Stefan ULg et al

in Research in Veterinary Science (2013), In press

Little is known about the effect of various animal's signalment variables on echocardiographic reference values in the equine species. This study was performed to evaluate the effect of sex, breed, age ... [more ▼]

Little is known about the effect of various animal's signalment variables on echocardiographic reference values in the equine species. This study was performed to evaluate the effect of sex, breed, age and body weight (BW) on echocardiographic measurements in the equine species. Echocardiography was performed on 212 ponies or horses of various breeds, aged from 1 day to 37 years old (mean ± SD: 7.8 ± 5.8 years), BW 38-890 kg (mean ± SD: 421 ± 133 Kg), and free of cardiac disease. Fifty of those animals aged from 2 months to 35 years old (mean ± SD: 11.6 ± 6.4 years old); BW 77-662 kg (mean ± SD: 436 ± 135 kg) were also examined using the pulsed-wave Doppler mode. Standard two-dimensional and M-mode echocardiography were performed on all animals. Standard pulsed-wave Doppler examination of each cardiac valve was performed on the 50 first examined animals. Data were analysed using a general linear model including the effect of sex, age, breed and BW after logarithmic transformation of the data. Therefore, the same analysis was performed separately on animals aged  2 years-old and on older animals. All dimensional echocardiographic measurements were significantly affected by BW and most of them were significantly affected by breed, but not by sex. Only the aortic and the pulmonary artery internal diameter were significantly affected by age. None of the Doppler measurements were significantly affected by the tested variables. In conclusion, in the equine species, dimensional echocardiographic reference values should be established using regression equations as a function of BW, which could increase the diagnostic value of this leading technique in equine cardiology. Breed could also have an effect on those measurements. [less ▲]

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See detailINFLUENCE OF MYELOPEROXIDASE ACTIVITY ON EQUINE POST-THAW SEMEN QUALITY
Ponthier, Jérôme ULg; Franck, Thierry ULg; Parrilla Hernandez, Sonia ULg et al

in Edeas, Marvin (Ed.) Proceedings of the 2nd ISANH World congress on Fertility and Antioxidants (2012, December 06)

This study confirms that active MPO is associated with cellular fraction of the ejaculate, as previously suggested for total MPO concentration in thawed semen (2). However, active MPO concentrations were ... [more ▼]

This study confirms that active MPO is associated with cellular fraction of the ejaculate, as previously suggested for total MPO concentration in thawed semen (2). However, active MPO concentrations were dramatically lower than total MPO concentrations observed in equine semen (3), which could be explained by presence of inactive MPO subunits in semen. MPO activity in sperm-rich pellet can be used as a predictive marker of post-thaw semen quality. Moreover, methods to inhibit MPO should be investigated in semen. [less ▲]

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See detailImmature equine oocyte vitrification
Gatez, Carine ULg; Ectors, F.J.; Farnir, Frédéric ULg et al

Scientific conference (2012, October 19)

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See detailRepeatability, variability and reference values of pulsed wave Doppler echocardiographic measurements in healthy Saanen goats.
Leroux, Aurélia ULg; Farnir, Frédéric ULg; Moonen, Marie ULg et al

in BMC Veterinary Research (2012), 8

Background Pulsed wave (PW) Doppler echocardiography has become a routine non invasive cardiac diagnostic tool in most species. However, evaluation of intracardiac blood flow requires reference values ... [more ▼]

Background Pulsed wave (PW) Doppler echocardiography has become a routine non invasive cardiac diagnostic tool in most species. However, evaluation of intracardiac blood flow requires reference values, which are poorly documented in goats. The aim of this study was to test the repeatability, the variability, and to establish the reference values of PW measurements in healthy adult Saanen goats. Using a standardised PW Doppler echocardiographic protocol, 10 healthy adult unsedated female Saanen goats were investigated three times at one day intervals by the same observer. Mitral, tricuspid, aortic and pulmonary flows were measured from a right parasternal view, and mitral and aortic flows were also measured from a left parasternal view. The difference between left and right side measurements and the intra-observer inter-day repeatability were tested and then the reference values of PW Doppler echocardiographic parameters in healthy adult female Saanen goats were established. Results As documented in other species, all caprine PW Doppler parameters demonstrated a poor inter-day repeatability and a moderate variability. Tricuspid and pulmonary flows were best evaluated on the right side whereas mitral and aortic flows were best obtained on the left side, and reference values are reported for healthy adult Saanen goats. Conclusions PW Doppler echocardiography allows the measurement of intracardiac blood flow indices in goats. The reference values establishment will help interpreting these indices of cardiac function in clinical cardiac cases and developing animal models for human cardiology research. [less ▲]

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See detailThe effect of homologous prostatic fluid on the parameters of dog semen extended and frozen in Tris-egg yolk and INRA Freeze: preliminary results
Stanescu, Pascal; Van Den Berghe, Femke ULg; Aissi, Adel et al

in Reproduction in Domestic Animals (2012, August), 47(s4), 431

Recent studies showed that the cryoprotector component of egg yolk for the freezing of canine semen is represented by the low density lipoproteins (LDL), while the others components of egg yolk have a ... [more ▼]

Recent studies showed that the cryoprotector component of egg yolk for the freezing of canine semen is represented by the low density lipoproteins (LDL), while the others components of egg yolk have a number of detrimental effects on the frozen-thawed canine semen. The aim of this study was to compare the effect of a LDL commercial extender used for stallion semen – INRA FreezeÒ (IMV Technologies, L¢Aigle, France) with a standard extender for dog semen containing Tris, fructose, glycerol and 20% egg yolk (TFG-EY). Since there are some conflicting reports concerning the effect of prostatic fluid on sperm function, we also investigated if the effect of post-thaw dilution with autologous prostatic fluid on viability, motility and acrosome status of frozen-thawed canine spermatozoa is correlated with the composition of the extender. Semen was collected from six Beagle dogs and each sperm rich fraction was divided in two parts: one was diluted with INRA FreezeÒ and the other with TFG-EY and frozen with the same protocol. The third fraction of each ejaculate was collected separately, centrifuged at 1118 · g for 10 min and the supernatant was frozen at )18°C until use. For each extender, two straws were thawed: one straw was diluted 1:2 with autologous prostatic fluid, while the other was not diluted at all. Motility (CASA, IVOS; Hamilton Thorne, USA), viability and acrosome status (Guava EasyCyteÒ flow cytom- eter; IMV Technologies, L¢Aigle, France), morphology (Diff-Quick stain) were assessed at 5 min, 1 h and 2 h post-thaw (T0, T1, T2). Statistical analyses were performed with IBM SPSS software (ver. 19 for Windows; IBM, New York, NY, USA). The results are presented as mean values and a p value <0.05 was considered statistically significant. Viability, total and progressive motility and the percentage Ó 2012 Blackwell Verlag GmbH 432 Abstracts of intact acrosomes were higher (p < 0.05) for the TFG-EY group with or without the addition of the prostatic fluid. The results for motility in the INRA Freeze group were dramatically low compared to the TFG-EY group. There were no significant differences regarding the morphology of fresh and frozen semen for any of the four groups. Addition of prostatic fluid for the TFG-EY group significantly reduced the total and progressive motility at T0, T1 and T2 (p < 0.05), but showed no influence for the INRA Freeze group. This data show that the effect of prostatic fluid regarding motility differs between the two extenders. This may illustrate either an inhibitory effect of some components of the INRA FreezeÒ on the noxious effects of the prostatic fluid or conversely an enhancing effect of the TFG-EY on those. Alternatively, it could be that low values obtained with INRA FreezeÒ did not permit highlighting the effects of prostatic fluid. [less ▲]

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See detailSquamous metaplasia of the prostate and diffuse alopecia in a 13-year-old castrated dog due to chronic ingestion of exogenous estradiol
Van Den Berghe, Femke ULg; Ponthier, Jérôme ULg; Deleuze, Stefan ULg

in Reproduction in Domestic Animals (2012, August), 47(S4), 561

Introduction. A 13-year old whippet with a generalized progressive alopecia since 2 years was referred to our clinic to perform a low-dose dexamethasone stimulation test and abdominal ultrasound to ... [more ▼]

Introduction. A 13-year old whippet with a generalized progressive alopecia since 2 years was referred to our clinic to perform a low-dose dexamethasone stimulation test and abdominal ultrasound to exclude or confirm Cushing disease. The dog had previously been treated with trilostane for 4 months without having any positive effects. Earlier blood analysis showed no significantly abnormalities and ACTH stimulation test was negative. Having bilateral cryptorchidism, the dog was castrated at young age. Clinical findings and treatment. Abdominal ultrasound revealed a slightly enlarged right adrenal gland (diameter of 1 cm at the cranial part). The prostate was enlarged and heterogeneous with multiple anechogenic cavities, indicating hormonal stimulation. Craniad the prostate and ventrally of the bladder, a hypoechogenic, oval structure (3 cm by 1.7 cm) could be seen, and was suspected to be a remaining cryptorchide testicle. Fine needle aspiration however showed that the structure contained a thick purulent liquid, with large amounts of polymorhonuclear white blood cells. Blood analysis revealed a leukocytosis (29370/mm3) with neutrophilia (23202/mm3). Low-dose dexamethasone test definitely excluded Cushing disease (basal cortisol levels 2,17 µg/dl; cortisol at 4 and 8 hours < 1 µg/dl). A prostatic wash was performed and revealed a severe prostatitis and presence of keratinized prostatic cells, indicating a squamous metaplasia of the prostate. Measurement of seric LH levels (LH Witness®, Synbiotics) showed an LH concentration lower than 1 ng/ml, indicating a hormonal negative feedback on the hypothalamo-hypophyso-gonadal axis. After a more thorough anamnesis, it became clear that the dog was licking and ingesting a transdermal estradiol containing cream (Estrogel®) from his owner, since two years, causing his symptoms. The dog was put on antibiotic treatment for the prostatitis (enrofloxacine 10 mg/kg SID) and underwent surgery to excise the abscess cranial of the prostate. The two ductuli deferentes were attached to this structure. Histopathological analysis revealed this tissue to be embryological remanents or a morphological anomaly with a urogenital origin. Control after 4 weeks showed that the prostate slightly decreased in volume, however, anechogenic cavities where still present of which one increased in volume. The alopecia was still present as well, both probably due to a prolonged action of the estrogens. Conclusion. Even though injectable estrogen preparations are not longer available in veterinary medicine in Europe, a thorough anamnesis towards other exogenous estrogen sources is still necessary and can reduce the number of supplementary exams performed. [less ▲]

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See detailA flow cytometric study on the effect of myeloperoxidase on stallion spermatozoal motility and structure
Ponthier, Jérôme ULg; Parrilla Hernandez, Sonia ULg; Van Den Berghe, Femke ULg et al

in Journal of Equine Veterinary Science (2012, August), 32(8), 509

Myeloperoxidase (MPO) is a pro-oxidant enzyme that is normally contained in neutrophils. MPO has recently been associated with keratinized cells and with decreased post-thaw motility in stallion semen [1 ... [more ▼]

Myeloperoxidase (MPO) is a pro-oxidant enzyme that is normally contained in neutrophils. MPO has recently been associated with keratinized cells and with decreased post-thaw motility in stallion semen [1]. The aim of the study was to determine effects of experimental addition of active MPO on motility, mitochondrial potential, apoptosis induction, membrane and acrosome integrity in equine semen. Three stallions were used and semen was collected four times. Extended (INRA96TM) semen was processed for density gradient centrifugation (Equipure Bottom Layer®) [2]. Purified pellet was re-extended to 100x106spermatozoa/ml in INRA96TM and divided in 3 samples. One sample was used for control and active human MPO (Calbiochem, Merck) was added in the other two samples to final concentration of 5 or 50 ng/ml. After incubation (2 hours, 20°C), motility was analysed with Computer Assisted Semen Analysis (IVOS, Hamilton-Throne, Beverly, MA, USA) and cytometric analyzes were perfomed with EasyCyte (IMV). Mitochondrial potential and apoptosis were assayed using Guava Mitopotential JC-1 and 7-AAD kit (Millipore). Membrane and acrosome integrity were respectively assayed with PI (Propidium Iodide) (Invitrogen) and PNA (Peanut Agglutinin-Fluorescein Iso Thio Cyanate) (Sigma-Aldrich). Statistical differences (p<0.05) were determined using Kruskall-Wallis test. No effect of the stallions was observed on parameters assayed in this study. Unlike total motility, progressive motility was decreased in both MPO concentrations (p<0.001). MPO addition had no effect on membrane and acrosome integrity. No differences were detected for the percentages of spermatozoa having polarised or depolarised mitochondria. Apoptosis, assayed by 7-AAD fluorescence, was not increased by the treatments. Our results agree with previously published effects of in vitro ROS production systems with xanthine oxidase [3], showing an effect on motility but no influence on mitochondria and membrane or acrosome integrity. However, membrane lipoperoxidation was increased by ROS in this study [3], and it could be linked to the impaired motility also observed in our protocol. Further studies with increasing concentrations of added MPO should be conducted to correlate motility with lipoperoxidation. References [1] Ponthier J, Desvals M, Franck T, de la Rebiere de Pouyade G, Spalart M, Palmer E, Serteyn D, Deleuze S. Myeloperoxidase in equine semen: Concentration and Localization during freezing processing. Journal of Equine Veterinary Science 2012;32: 32-37. [2] Edmond AJ, Teague SR, Brinsko SP, Comerford KL, Waite JA, Mancill SS, Love CC, Varner DD. Effect of density-gradient centrifugation on quality and recovery rate of equine spermatozoa. Animal Reproduction Science 2008;107: 318-318. [3] Baumber J, Ball BA, Gravance CG, Medina V, Davies-Morel MC. The effect of reactive oxygen species on equine sperm motility, viability, acrosomal integrity, mitochondrial membrane potential, and membrane lipid peroxidation. J Androl 2000;21: 895-902. [less ▲]

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