References of "Deleuze, Stefan"
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See detailCANINE GANGRENOUS MASTITIS : A CASE REPORT
Egyptien, Sophie ULiege; Lefebvre, Michaël ULiege; Guieu, Liz-Valéry ULiege et al

Poster (2017, July)

This report describes the treatments and discusses the cost effectiveness of a Negative Pressure Wound Therapy (NPWT) device on a gangrenous mastitis case. Mastitis is an inflammation of the mammary gland ... [more ▼]

This report describes the treatments and discusses the cost effectiveness of a Negative Pressure Wound Therapy (NPWT) device on a gangrenous mastitis case. Mastitis is an inflammation of the mammary gland found mainly in lactating females. Coliforms (Escherichia coli), Staphylococcus spp (Staphylococcus aureus) and, to a lesser extent, Streptococcus spp are the most commonly isolated organisms in bitches. The bitch can be presented because of local signs of inflammation, puppies failing to thrive or even severe septic shock. While more common in ruminants, gangrenous mastitis is rare in the bitch. It is mostly due to Staphylococcus aureus, a gram positive and catalase positive bacteria producing alpha hemolysin. This toxin binds to ADAM10, a transmembrane protein, forming pores in the cell membrane, causing an acute inflammation via intracellular calcium flow, leading to severe oedema, necrosis and gangrene. Loss of skin integrity also comes from the cleavage of E-cadherin by ADAM10 after its toxin-mediated activation. A 8.7kg, 3-year-old neutered female Sheltie farm dog was referred to the Veterinary Clinic of the University of Liège for suspicion of septic peritonitis after an ovariohysterectomy on 4 dead puppies 36 hours earlier. They died during long lasting dystocia. Amoxicillin-acid clavulanic and enrofloxacin antibiotherapy had been initiated during surgery. The dog was in decompensated septic shock. The surgical wound was not reactive and no sign of peritonitis was seen by abdominal ultrasonography. The right inguinal and the two most caudal left mammary glands were swollen, crackling with a patchy blue discoloration. An acute fulminant mastitis with gangrenous involvement and sepsis was diagnosed. Fluids and continuous rate infusion of norepinephrine and dobutamine were administered to control severe hypotension. Metronidazole was added since Clostridium spp could not be excluded. Debridement was started as soon as the arterial pressure was stabilised and the wound was disinfected with hydrogen peroxyde for the 3 first days. While hydrogen peroxyde is indicated for catalase negative infections, the already started antibiotherapy prevented bacterial identification and sensitivity determination. Alginate-honey patches replaced wet to dry bandages after 4 days. After 7 days of progressive debridement, the wound was 15cm long, 13cm wide and 1.2cm deep. A NPWT device was placed under anaesthesia after extensive debridement. It was kept in place for one week with one renewal after 2 days under slight sedation. The wound was then surgically closed. Cutaneous sutures were removed 10 days later as cicatrization was satisfactory. NPWT is indicated in open wound management, for infection control and for stimulating granulation tissue production prior to reconstruction. It increases contraction in deep, three dimensional wounds. Closure rate is significantly shorter with NPWT than with standard wet to dry bandages. Cost of NPWT is comparable to standard bandages but more comfortable for the patient. Standards bandages require daily changes, sometimes for several weeks and under repeated anaesthesias, which may be debilitating for the patient and expensive for the owner. Conversely, NPWT is changed only once and allows earlier surgical closure of the wound. The main complication is vacuum loss, which is surgeon’s experience dependant. [less ▲]

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See detailCollecte in vivo d’ovocytes d’ânesses et chronologie de la maturation in vitro
Reigner, Fabrice; Barrière, Philippe; Blard, Thierry et al

in Pratique Vétérinaire Equine (2017), 49(193), 38-43

La plupart des espèces d’ânes sauvages et domestiques est en danger d’extinction. Leur préservation nécessite la cryoconservation du sperme, des ovocytes et des embryons. L’objectif de cette étude était ... [more ▼]

La plupart des espèces d’ânes sauvages et domestiques est en danger d’extinction. Leur préservation nécessite la cryoconservation du sperme, des ovocytes et des embryons. L’objectif de cette étude était de mettre au point une technique de collecte in vivo et de maturation in vitro des ovocytes d’ânesses. Les ovocytes ont été collectés par ponction folliculaire transvaginale sous guide échographique, placés dans un milieu de maturation in vitro pendant 24, 30, 34 ou 38 heures puis analysés. Quatre-vingt-douze ovocytes ont été collectés dans 193 follicules (48%) avec une moyenne de 4,2 ovocytes par ânesse. Après 34 heures de maturation in vitro, 45% des ovocytes ont été matures. Pour la première fois une technique de collecte in vivo et de maturation in vitro d’ovocytes d’ânesses a été développée. Cette technique permet d’envisager la cryoconservation et la fécondation in vitro de ces ovocytes. [less ▲]

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See detailAborder la fertilité chez l'étalon et la jument
Ponthier, Jérôme ULiege; Deleuze, Stefan ULiege

Conference given outside the academic context (2017)

Cette conférence se propose de définir quels sont les facteurs de fertilité chez la jument comme chez l'étalon et d'étudier les pathologies ou mauvaises pratiques pouvant mener à de l'infertilité dans l ... [more ▼]

Cette conférence se propose de définir quels sont les facteurs de fertilité chez la jument comme chez l'étalon et d'étudier les pathologies ou mauvaises pratiques pouvant mener à de l'infertilité dans l'espèce équine. [less ▲]

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See detailReference values of two-dimensional and M-mode echocardiographic measurements as a function of body size in various equine breeds and in ponies.
Al-Haidar, Ali; Moula, Nassim ULiege; Leroux, Aurélia ULiege et al

in Journal of Veterinary Cardiology : The Official Journal of the European Society of Veterinary Cardiology (2017)

Introduction: The aim of this study was to establish echocardiographic reference values for the equine species using allometric regression equations based on body weight (BW) and thoracic circumference ... [more ▼]

Introduction: The aim of this study was to establish echocardiographic reference values for the equine species using allometric regression equations based on body weight (BW) and thoracic circumference (TC). Animals: A total of 239 horses or ponies were studied, including 65 warmbloods, 33 Standardbreds, 41 Thoroughbreds, 32 Arabian horses, 28 draft horses and 40 ponies aged from 1 day to 30 years, weighing from 18 to 890 kg, with no evidence of cardiac disease. Methods: For each horse or pony, a two-dimensional and M-mode echocardiography was performed. Within each breed, the relationships between BW or TC and echocardiographic dimensions were examined using power regression equations. Predictions and their 95% prediction intervals were calculated for the echocardiographic measurements. Results: Within each breed, all echocardiographic measurements showed a significant and positive relationship with a high coefficient of determination for the estimation of the regression equations using BW and TC as the main explanatory variables. Breed-specific power regression equations as well as the 95% prediction intervals were calculated for each echocardiographic measurement as a function of BW and TC. Conclusions: In the future, the body size-corrected and breed-specific echocardiographic reference values calculated in the present study could be used to discriminate between normal and abnormal values in a given animal. [less ▲]

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See detailAnalysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes
Franciosi, Federica; Tessario, Irene; Dalbies-Tran, Rozenn et al

in Journal of Visualized Experiments (2017), 123(e55242),

The field of assisted reproduction has been developed to treat infertility in women, companion animals, and endangered species. In the horse, assisted reproduction also allows for the production of ... [more ▼]

The field of assisted reproduction has been developed to treat infertility in women, companion animals, and endangered species. In the horse, assisted reproduction also allows for the production of embryos from high performers without interrupting their sports career and contributes to an increase in the number of foals from mares of high genetic value. The present manuscript describes the procedures used for collecting immature and mature oocytes from horse ovaries using ovum pick-up (OPU). These oocytes were then used to investigate the incidence of aneuploidy by adapting a protocol previously developed in mice. Specifically, the chromosomes and the centromeres of metaphase II (MII) oocytes were fluorescently labeled and counted on sequential focal plans after confocal laser microscope scanning. This analysis revealed a higher incidence in the aneuploidy rate when immature oocytes were collected from the follicles and matured in vitro compared to in vivo. Immunostaining for tubulin and the acetylated form of histone four at specific lysine residues also revealed differences in the morphology of the meiotic spindle and in the global pattern of histone acetylation. Finally, the expression of mRNAs coding for histone deacetylases (HDACs) and acetyl-transferases (HATs) was investigated by reverse transcription and quantitative-PCR (q-PCR). No differences in the relative expression of transcripts were observed between in vitro and in vivo matured oocytes. In agreement with a general silencing of the transcriptional activity during oocyte maturation, the analysis of the total transcript amount can only reveal mRNA stability or degradation. Therefore, these findings indicate that other translational and post-translational regulations might be affected. Overall, the present study describes an experimental approach to morphologically and biochemically characterize the horse oocyte, a cell type that is extremely challenging to study due to low sample availability. However, it can expand our knowledge on the reproductive biology and infertility in monovulatory species. [less ▲]

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See detailCase report: Ovarian fibroma in a mare – Hormonal considerations
Ponthier, Jérôme ULiege; Salciccia, Alexandra ULiege; Parrilla Hernandez, Sonia ULiege et al

in Reproduction in Domestic Animals (2016, October 27), 51(supplement 2), 132

This report shows that, as in humans, AMH as well as steroids productions are low in case of ovarian fibroma, thus preserving normal cyclicity.

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See detailAssessment of Myeloperoxidase activity in raw equine fresh semen
Ponthier, Jérôme ULiege; Evra, Adeline; Parrilla Hernandez, Sonia ULiege et al

Poster (2016, June 20)

In previous studies [1], myeloperoxidase (MPO), a pro-oxidant enzyme, has been shown to be partially inactivated in commercial equine semen extenders. The aim of this study was to determine MPO ... [more ▼]

In previous studies [1], myeloperoxidase (MPO), a pro-oxidant enzyme, has been shown to be partially inactivated in commercial equine semen extenders. The aim of this study was to determine MPO concentration and activity in fresh equine semen and in supernatant of semen diluted with a cristalloïd solution after cushionned centrifugation. Three stallions have been collected 5 times at 2 day-intervals. Semen concentration was assessed with Nucleo Counter sp100. A sample containing 100x106spz was then immediatly frozen (-80°C) before assays. A volume of semen containing 500x106spz was diluted 3 times with PBS (1v/3v). Samples were centrifuged (1000xg, 20 minutes). One milliliter of supernatant was sampled and stored at -80°C before assays. Total MPO concentration was assayed by commercial enzyme-linked immuno-sorbent assay and active MPO concentration was assayed by specific immunological extraction followed by enzymatic detection as previously described in our earlier paper [1]. As all values were not normally distributed, Kruskall-Wallis test was used to analyse differences between medians and Spearman test was used to determine correlations. Median total and active MPO concentrations in raw semen were respectively 580500ng/mL and 1.098ng/ mL. Median total and active MPO concentration in semen supernatant extended with PBS (1v/3v) were respectively 107500ng/mL and 0.236ng/mL. In raw semen, total and active MPO concentrations were highly correlated (r=0.7096; p=0.0030). However, in semen supernatant extended with PBS (1v/3v), they were not (r=0.2121; p=0.4479). Total MPO concentrations were similar to those observed in our previous papers [1]. When concentration observed in supernatant is compared to that observed in total raw semen (cells and supernatant), we observe that MPO in raw semen is higher than in supernatant, even after correction for dilution. This confirms that cellular part of semen contains MPO and releases it during cold-chock. Without any large proteins in the extender medium, MPO activity can be assessed in raw semen and in its supernatant. Active MPO concentrations are very low when compared to total MPO concentration, (nearly one million times). Concentration of active MPO in supernatant corrected for dilution was also lower than concentration observed in raw complete semen, also confirming active MPO release by cellular part of semen. Association of total and active MPO concentration is supporting this observation. This study confirms our previous assessments [1] that active and total MPO are released by non sperm-cells during cold shock. [less ▲]

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See detailChronology of in vitro maturation of donkey (Equus asinus) oocytes collected by ovum pick up
Douet, Cécile; Goudet, Ghylène; Kaabouba-Escourier, Aurore et al

Poster (2016, June)

Most wild donkey breeds are currently endangered, such as the Asiatic and the African wild ass. Moreover, many domestic donkey breeds are vulnerable to extinction, such as the Martina Franca donkey in ... [more ▼]

Most wild donkey breeds are currently endangered, such as the Asiatic and the African wild ass. Moreover, many domestic donkey breeds are vulnerable to extinction, such as the Martina Franca donkey in Italy, the Zamorano-Leonés donkey in Spain and the Normand donkey in France. Their preservation includes the creation of a Genome Resource Bank. Embryos cryopreservation allows the preservation of genetics from both male and female and is the fastest method to restore a breed. However, embryo production in vivo is limited in equids. In vitro production of embryos would allow the production of several embryos per cycle, but no technique has yet been developed in the donkey for in vitro production of embryos. In that context, ovum pick up (OPU) would come as a very helpful tool both for embryo production and research purposes. Our objective was to develop OPU in jennies and to analyze the chronology of in vitro maturation of donkey oocytes. Donkey cumulus-oocyte complexes (COCs) were collected by OPU from 5 to 25mm follicles in adult cyclic jennies. During OPU, jennies were injected detomidine, butorphanol, and butylscopolamine for sedation, analgesia and antispasmodia. COCs were in vitro matured in TCM199 supplemented with fetal calf serum and epidermal growth factor for 24, 30, 34 or 38 hours in groups of 10 to 30 in an atmosphere of 5% CO2 in air at 38.5°C. Five OPU sessions were performed, with 3 to 6 females per session. We collected a total of 92 COCs out of 193 follicles (48%) with an average of 4.2 COCs per jenny. The recovery rate per follicle increased along the OPU sessions: 34%, 43%, 52%, 47% and 56%, probably due to the increasing skill of the staff. Despite a very large experience with OPU in horses, all staff members required some time to adjust to the specificities of the jenny vs mare, in particular to the differences in holding the ovary. All COCs were expanded after over 24 hours in vitro maturation (IVM). At collection, jennies COCs contained a germinal vesicle. After 24 hours IVM, COCs contained either a germinal vesicle or condensed chromatin. Metaphase 1 oocytes were observed after 30 hours IVM and 45% were in metaphase 2 after 34 hours IVM. Half of the COCs were degenerating after 38 hours IVM. In conclusion, we established for the first time conditions for ovum pick up in jennies and the chronology of in vitro maturation of donkey oocytes. We showed that in vitro maturation of donkey oocytes can produce 44% of metaphase 2 oocytes after 34 hours in culture while most equine oocytes reach metaphase 2 after 30 hours in culture. [less ▲]

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See detailCan serum progesterone be used to decide altrenogest supplementation discontinuation in problem pregnancies in mares?
Parrilla Hernandez, Sonia ULiege; Delahaut, Philippe; Ponthier, Jérôme ULiege et al

Poster (2016, June)

Use of oral altrenogest as exogenous progesterone (P4) in mares in an effort to maintain high-risk pregnancies is a widespread practice. Treatment is sometimes initiated as early as 2 days after ovulation ... [more ▼]

Use of oral altrenogest as exogenous progesterone (P4) in mares in an effort to maintain high-risk pregnancies is a widespread practice. Treatment is sometimes initiated as early as 2 days after ovulation. Timing when it can safely be stopped is frequently uncertain and therefore, supplementation is often continued until 100-120 days, when the placental secretion of progestagens has taken over. The aim of this study was to determine if altrenogest interfered with progesterone assay and if timing of altrenogest administration discontinuation could be based on the blood concentration of P4. Data were obtained from 7 healthy standard breed mares in seasonal anoestrus and in dioestrus. Mares were considered anoestrus when no follicles larger than 15 mm in diameter or corpus luteum (CL) were found at 2 consequent ultrasound examinations 7 days apart. They were confirmed dioestrus based on an observed ovulation and the presence of a CL 2 days after. Mares were treated with 0.044 mg/kg of altrenogest (Regumate®, Intervet, Boxmeer, Netherlands) orally once per day during 12 days beginning at any time for the anestrus group and at day 2 after ovulation for the dioestrus group. Blood was collected at day 0, 6, 12, 18 right before altrenogest administration and the serum was stored at -20°C until analysis. P4 concentration was obtained by radioimmunoassay and altrenogest by liquid chromatography-mass spectrometry. Differences in P4 and in altrenogest concentrations between different sampling days were determined by Friedman non parametric test. Concentrations of altrenogest before treatment were almost not detectable (negative control). At day 6, they were significantly higher (p<0.05), and were back to basal, almost not detectable values at day 18, in both groups. P4 concentration was basal with values < 0.4 ng/ml all through the experiment in the anoestrus group. In the dioestrus group, P4 concentration was variable from one mare to another, but it was significantly higher in mid-dioestrus (day 6) as compared to the time of the impending next oestrus (day12). No cross-reaction between P4 and altrenogest assays was observed as illustrated by the fact that concentration of P4 during anoestrus remained basal with or without supplementation. Despite the treatment, the variation of P4 over time was as expected in both groups. Altrenogest was no longer detectable 6 days after the last administration. Further studies with larger numbers and sampling in dioestrus to compare P4 levels in treated and untreated mares to investigate the potential effect of altrenogest on endogenous P4 production by the CL are needed. However, our experiment shows that P4 levels assayed by RIA can be trusted to evaluate if the secondary CL’s have become functional and can thus maintain the pregnancy while oral altrenogest supplementation is discontinued. [less ▲]

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See detailBiotechnolgia w rozrodzie koni
Deleuze, Stefan ULiege; Gajewski, Zdzislaw; Faundez, Ricardo et al

in Rozrod koni, Wybrane aspekty IV Monograph (2016)

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See detailExploration fonctionnelle du système reproducteur mâle.
Deleuze, Stefan ULiege; Ponthier, Jérôme ULiege

in Bardiès, Jacques (Ed.) Manuel pratique de thérapeutique équine (2014)

Les tests hormonaux sont utiles pour diagnostiquer si il existe du tissu testiculaire dans l’animal présenté. Ils interviennent donc dans le diagnostic différentiel du cheval présentant un caractère ... [more ▼]

Les tests hormonaux sont utiles pour diagnostiquer si il existe du tissu testiculaire dans l’animal présenté. Ils interviennent donc dans le diagnostic différentiel du cheval présentant un caractère d’étalon mais qui n’a pas de testicule apparent. Ce diagnostic différentiel comprend : - le cheval hongre ; - les cas de cryptorchidies bilatérales ; - le cheval castré unilatéralement chez lequel le testicule cryptorchide a été laissé en place. Le but de ces tests hormonaux est de déterminer si il existe encore des sécrétions de molécules spécifiques du tissu testiculaire. [less ▲]

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See detailA case of true hermaphrodism in a horse
Parrilla-Hernadez, Sonia; de la Rebière de Pouyade, Geoffroy ULiege; Grulke, Sigrid ULiege et al

in Reproduction in Domestic Animals (2014, September), 49(suppl 3), 50

True hermaphroditism in horses, is a complex and poorly under- stood disorder of sexual development characterized by the presence of both male and female gonads as separate organs or in a combined ... [more ▼]

True hermaphroditism in horses, is a complex and poorly under- stood disorder of sexual development characterized by the presence of both male and female gonads as separate organs or in a combined structure called ovotestis. A 3-year-old Spanish nullipa- rous mare with a history of stallion behaviour and an abnormal reproductive exam was presented at the Equine Clinic of the University of Li ege. Externally, a small vulva with an enlarged clitoris was observed. Transrectal ultrasonography showed a thin (<1 cm) uterus, leading to a small heterogeneous structure in the normal place of the left ovary. No structure could be identified in the presumed location of the right ovary. Speculum exam was compatible with a vaginal agenesis, as the cervix opened craniad the urethral meatus. Oestradiol, progesterone and testosterone concen- trations were compatible with a stallion’s endocrinology. Gonads were laparoscopically found in the usual location of the ovaries in both flanks and excised. Histopathology of both gonads showed atrophic testicular tissue with hyperplastic Leydig cells. The left gonad also contained ovarian tissue with some scarce primordial follicles. Clitoral enlargement is the first symptom most commonly identified in animals with true hermaphroditism and can be explained by the production of testosterone by the gonads, however, the aetiology of the vaginal agenesis is unknown. True hermaph- roditism in horses is generally related to 64XX syndrome with or without SRY gene translocation (impending karyotyping analysis). After 5 days the mare was discharged from the clinic, and in absence of testosterone the stallion behaviour disappeared in a couple of months. [less ▲]

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See detailGlucose use and lactate production by equine fresh semen in human and equine extender
Ponthier, Jérôme ULiege; De Tullio, Pascal ULiege; Parrilla-Hernandez, Sonia et al

in Reproduction in Domestic Animals (2014, September), 49(suppl 3), 13

This study shows that this human semen extender doesn’t support equine semen preservation. Sperm cells’ glucose consumption and lactate production seem to be negligible, as these parameters were not ... [more ▼]

This study shows that this human semen extender doesn’t support equine semen preservation. Sperm cells’ glucose consumption and lactate production seem to be negligible, as these parameters were not affected by sperm concentrations in our study. Our results suggest that spermatozoa are able to cleave complex carbohydrates as glucose concentration in INRA96 increased over time. [less ▲]

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See detailHigh concentrations of Myeloperoxidase in the equine uterus as an indicator of endometritis
Parrilla Hernandez, Sonia ULiege; Ponthier, Jérôme ULiege; Franck, Thierry ULiege et al

in Theriogenology (2014), 81(7), 936-940

Intra-luminal fluid and excessive abnormal hyper-edema are regularly used for the diagnosis of endometritis in the mare, which is routinely confirmed by the presence of neutrophils on endometrial smears ... [more ▼]

Intra-luminal fluid and excessive abnormal hyper-edema are regularly used for the diagnosis of endometritis in the mare, which is routinely confirmed by the presence of neutrophils on endometrial smears. Studies show a relation between neutrophils and myeloperoxidase (MPO), an enzyme contained in and released by neutrophils during degranulation or after cell lysis. This enzyme has been found in many fluids and tissues and associated with different inflammatory pathologies in the horse. The aims of this study were to assess the presence and concentration of MPO in the equine uterus, and to investigate its relation with neutrophils, and other clinical signs of endometritis. Mares (n=51) were evaluated for presence of intra-luminal fluid and excessive endometrial edema before breeding, and a small volume lavage and cytology samples were obtained. From 69 cycles, supernatant of the uterine flushes was analysed with a specific equine MPO ELISA assay to measure MPO concentration. Cytology samples were used for the diagnosis of endometritis. MPO was present in the uterus of all estrus mares in highly variable concentrations. MPO concentrations were significantly (p<0.05) higher in samples with positive cytologies and in presence of intra-luminal fluid. Occasionally, some samples with negative cytologies showed high MPO concentration, but the opposite was never observed. Cycles presenting hyper-edema weren’t associated to high concentration of MPO, intra-luminal fluid or positive cytology making it a poor diagnostic tool of endometritis. [less ▲]

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See detailSperm Motility and Lactate production at different sperm concentrations
Ponthier, Jérôme ULiege; De Tullio, Pascal ULiege; Blommaert, Didier ULiege et al

in Journal of Equine Veterinary Science (2014, January), 34(1), 75-76

Lactate production is associated with total spermatozoa concentration. It negatively affects preservation of total and progressive motility, showing an effect of by-products of anaerobic metabolism on ... [more ▼]

Lactate production is associated with total spermatozoa concentration. It negatively affects preservation of total and progressive motility, showing an effect of by-products of anaerobic metabolism on long-term storage. Moreover, our data show that non-progressive motile spermatozoa are highly associated to lactate concentration, and thus, anaerobic glycolysis. More studies are required to determine relative contributions of aerobiosis and anaerobiosis to spermatozoa motility under different storage conditions. [less ▲]

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See detailDifferential expression and localization of glycosidic residues in in vitro- and in vivo-matured cumulus-oocyte complexes in equine and porcine species.
Accogli, Gianluca; Douet, Cecile; Ambruosi, Barbara et al

in Molecular reproduction and development (2014), 81(12), 1115-35

Glycoprotein oligosaccharides play major roles during reproduction, yet their function in gamete interactions is not fully elucidated. Identification and comparison of the glycan pattern in cumulus-oocyte ... [more ▼]

Glycoprotein oligosaccharides play major roles during reproduction, yet their function in gamete interactions is not fully elucidated. Identification and comparison of the glycan pattern in cumulus-oocyte complexes (COCs) from species with different efficiencies of in vitro spermatozoa penetration through the zona pellucida (ZP) could help clarify how oligosaccharides affect gamete interactions. We compared the expression and localization of 12 glycosidic residues in equine and porcine in vitro-matured (IVM) and preovulatory COCs by means of lectin histochemistry. The COCs glycan pattern differed between animals and COC source (IVM versus preovulatory). Among the 12 carbohydrate residues investigated, the IVM COCs from these two species shared: (a) sialo- and betaN-acetylgalactosamine (GalNAc)-terminating glycans in the ZP; (b) sialylated and fucosylated glycans in cumulus cells; and (c) GalNAc and N-acetylglucosamine (GlcNAc) glycans in the ooplasm. Differences in the preovulatory COCs of the two species included: (a) sialoglycans and GlcNAc terminating glycans in the equine ZP versus terminal GalNAc and internal GlcNAc in the porcine ZP; (b) terminal galactosides in equine cumulus cells versus terminal GlcNAc and fucose in porcine cohorts; and (c) fucose in the mare ooplasm versus lactosamine and internal GlcNAc in porcine oocyte cytoplasm. Furthermore, equine and porcine cumulus cells and oocytes contributed differently to the synthesis of ZP glycoproteins. These results could be attributed to the different in vitro fertilization efficiencies between these two divergent, large-animal models. [less ▲]

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See detailConcentration, Activity and Biochemical Characterization of Myeloperoxidase in Fresh and Post-thaw equine semen and their Implication on Freezability
Ponthier, Jérôme ULiege; Franck, Thierry ULiege; Parrilla Hernandez, Sonia ULiege et al

in Reproduction in Domestic Animals (2014), 49

Myeloperoxidase (MPO) is a pro-oxidant enzyme associated with decreased motility in thawed equine semen. This study aimed to describe MPO concentration, activity and subunits in raw and thawed semen and ... [more ▼]

Myeloperoxidase (MPO) is a pro-oxidant enzyme associated with decreased motility in thawed equine semen. This study aimed to describe MPO concentration, activity and subunits in raw and thawed semen and to correlate these data with motilities in raw and thawed semen. Semen samples from five stallions were collected four times. Motilities were assessed in raw and thawed semen. MPO assays were performed in raw seminal plasma, raw sperm-rich pellet and thawed semen. Total and active MPO concentrations were, respectively, assayed by enzyme-linked immunosorbent assay and specific immunological extraction followed by enzymatic detection. MPO subunits present in semen were characterized by Western 3 blot. Purified active MPO was added in PBS and freezing extender to control its activity during freezing procedure. Differences between medians were determined using Kruskal– Wallis test, and correlations were determined using Spearman’s test for nonparametric data. Active MPO concentration was low in seminal plasma and thawed semen, but high in pellet (p = 0.0058), as the opposite relation was observed for total MPO concentration (p < 0.0001). In seminal plasma and post- thaw semen, inactive 86-kDa MPO precursor was mainly observed. Purified MPO activity was decreased in the extender (p = 0.0286). MPO activity in pellet was highly correlated with thawed progressive motility (r = - 0.5576, p = 0.0086). Inac- tive MPO precursor and unknown low molecular weight inactive MPO precursor subunits explain low MPO activity in semen. Major MPO activity was observed in pellet, and post- thaw loss of activity is partially explained by MPO inactiva- tion in extender. Thawed semen motility was negatively correlated with MPO activity in pellet, becoming a potential freezability predictor. [less ▲]

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See detailCongélation de sperme dans l'espèce équine: état des lieux et perspectives
Ponthier, Jérôme ULiege; van den Berghe, Femke; Parrilla Hernandez, Sonia ULiege et al

in Annales de Médecine Vétérinaire (2014), 159(1), 56-71

Le but de cette revue est de décrire les techniques de congélation de la semence équine. Un rappel résumera la formation du spermatozoïde dans le testicule, son anatomie et sa physiologie en insistant sur ... [more ▼]

Le but de cette revue est de décrire les techniques de congélation de la semence équine. Un rappel résumera la formation du spermatozoïde dans le testicule, son anatomie et sa physiologie en insistant sur les structures lésées pendant le processus de congélation et décongélation. Les techniques de congélation du sperme seront ensuite résumées dans différentes espèces et comparées avec celles utilisées pour la semence équine. Les critères actuels de qualité seront définis pour le sperme avant et après congélation en insistant sur la mobilité totale, progressive et les différentes définitions de ces concepts dans la littérature. Les autres méthodes d’analyse comme la cytométrie de flux seront présentées ainsi que leurs intérêts cliniques. Les prochains développements et progrès dans le domaine de la congélation seront enfin envisagés : la prédiction de la qualité du sperme congelé à partir du sperme frais, l’amélioration des dilueurs et la lutte contre les formes activées de l’oxygène. [less ▲]

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