References of "Deleu, Magali"
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See detailSpectrofluorimetric and Zeta potential studies of alkylbetainate chloreide surfactants interaction with model membranes
Nsimba Zakanda, Francis; Lins, Laurence ULg; Razafindralambo, Hary et al

in Journal of Colloid Science and Biotechnology (in press)

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See detailRe-visiting plant plasma membrane lipids in tobacco: a focus on sphingolipids
Cacas, Jean Luc; Buré, Corinne; Grosjean, Kevin et al

in Plant Physiology (2016), 170

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See detailExperimental and in silico approaches to study the interaction of Remorin with plant plasma membrane : specific interaction of the C-term domain with lipids
Deleu, Magali ULg; Nasir, Mehmet Nail ULg; Gronnier, Julien et al

Conference (2015, September 29)

The function of Remorins, a diverse family of plant-specific proteins (1) is far to be fully elucidated. One of them, StREM1.3 (for Solanum tuberosum Remorin from group 1, homolog 3) has been reported to ... [more ▼]

The function of Remorins, a diverse family of plant-specific proteins (1) is far to be fully elucidated. One of them, StREM1.3 (for Solanum tuberosum Remorin from group 1, homolog 3) has been reported to regulate cell-to-cell propagation of the potato virus X (2). It was also shown to be localized to the inner leaflet of plasma membranes (PMs) and along plasmodesmata, bridges connecting neighbor cells essential for cell-to-cell communication in plants (3). The mechanisms driving StREM1.3 association with PM is still an open question. It was shown recently that a domain of 28 residues at the C-terminus of the potato (RemCA) is required and sufficient for anchoring to the PM (4). Here we combined experimental and in silico biophysics to unravel the molecular bases of RemCA membrane binding. Biomimetic membrane models of plant PM such as monolayers and liposomes were used with various biophysical techniques (Langmuir monolayer technique, Fourier-transformed infrared spectroscopy, circular dichroïsm) and modeling tools (home-made methods and molecular dynamics) (5) to answer to three questions: (i) What is the conformation adopted by RemCA within a membrane?, (ii) Is there any membrane lipid specificity in the RemCA-membrane binding? (iii) What is the role of the two different RemCA domains in the interaction? Results show that RemCA displays a preference for plant phosphoinositide and sitosterol-enriched inner leaflet plasma membrane rafts. Within the membrane, the C-terminal and the N-terminal domains adopt a random coil and a -helical conformation respectively. The C-terminal domain acts as a driver to bind RemCA to the membrane while the N-terminal domain stabilizes the peptide at the membrane. Lysine residues have a crucial importance in this interaction. References (1) Raffaele et al., Plant Physiol., 2007, 145: 593–600 (2) Raffaela et al., Plant Cell, 2009, 21: 1541–1555. (3) Maule, Curr. Opin. Plant Biol., 2008, 11: 680–686. (4) Perraki et al., Plant Physiology, 2012, 160 : 624-637. (5) Deleu et al., Biochim. Biophys. Acta – Biomembranes, 2014, 1838 : 3171-3190. [less ▲]

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See detailElicitor screening to protect wheat against Zymoseptoria tritici
Le Mire, Géraldine ULg; SIAH, ALI; Deleu, Magali ULg et al

Conference (2015, August 27)

Plants face an array of biotic and abiotic stresses in their environment, making it necessary to use various chemical inputs to maintain satisfactory yield. Today, conventional agriculture is evolving ... [more ▼]

Plants face an array of biotic and abiotic stresses in their environment, making it necessary to use various chemical inputs to maintain satisfactory yield. Today, conventional agriculture is evolving towards more sustainable practices, out of respect for human health and the environment. Elicitors are considered as promising biological control tools and draw major interest in IPM strategies. These plant-immunity triggering compounds, also called “stimulators of plant natural defenses”, induce a general and systemic resistance in the plant to various diseases. Although numerous elicitors have already been identified and some of them reached the market since the late 1970s, further investigations are still required to better understand the mode of action of these molecules in the plant and ensure a consistent efficiency under various field conditions. Few elicitors have yet been successfully tested and formulated to protect monocotyledonous crop plants such as wheat, which is cultivated over large areas in Europe. This study focuses on the screening of ten potential elicitor products of various origins and structures to protect winter wheat against the fungal pathogen Zymoseptoria tritici. Greenhouse trials were carried out to measure the ability of the different products to reduce disease foliar symptoms (necrosis, chlorosis and pycnidia). Topical spraying treatments with 3 different concentrations of each product were carried out 5 days before pathogen inoculation. Disease severity (% of symptoms on the total surface of the third leaf) was then scored every 2 days up to 28 days post-inoculation. In addition, phytotoxicity and biocide activity of these products was evaluated under greenhouse and laboratory conditions, respectively. The corresponding results will be presented and discussed with the perspective to choose the best elicitor candidates and to undertake investigations on the signaling pathway and the influence of environmental parameters on the elicitation capacity. [less ▲]

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