References of "Delaplace, Pierre"
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See detailIdentification of Metabolic Pathways Expressed by Pichia anomala KH6 in the Presence of the Pathogen Botrytis cinerea on Apple: New Possible Targets for Biocontrol Improvement
Kwasiborski, Anthony; Bajji, Mohammed; Renaut, J. et al

in PLoS ONE (2014), 9(3),

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See detail10. Perspectives - 2. Perspectives offertes par la culture en association de froment et de pois protéagineux d'hiver
Pierreux, Jérome ULg; Delaplace, Pierre ULg; Roisin, Christian et al

in Bodson, Bernard; Destain, Jean-Pierre (Eds.) Livre Blanc céréales (2014, February 26)

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See detailRhizobacterial volatile organic compounds implication in Brachypodium distachyon response to phosphorus deficiency
Baudson, Caroline ULg; Saunier de Cazenave-Mendaluk, Magdalena ULg; du Jardin, Patrick ULg et al

Poster (2014, February 07)

In agriculture, phosphorus (P) is considered as the second most growth-limiting macronutrient after nitrogen. However, P fertilizers are produced from non-renewable resources. In this context, sustainable ... [more ▼]

In agriculture, phosphorus (P) is considered as the second most growth-limiting macronutrient after nitrogen. However, P fertilizers are produced from non-renewable resources. In this context, sustainable production strategies have to be developed to enhance P use efficiency of crops, e.g. based on naturally occurring biotic interactions that limit the negative impacts of P deficiency in soils. Plant growth-promoting rhizobacteria (PGPR) have already revealed their ability to promote plant growth and tolerance to abiotic stresses through many mechanisms. Among them, the bacterial volatile organic compounds-mediated communication between plants and PGPR is still poorly documented. Our research project aims at studying the capacity of a model cereal plant (Brachypodium distachyon (L.) Beauv. Bd21) to face P deficiency in interaction with PGPR. The prerequisite of this project consists in characterizing Bd21 response to P deficiency by measuring plant biomass production and allocation, root system architecture, total phosphorus content, root-secreted and intracellular acid phosphatase activity under various P concentrations. Those results will allow us to define P-limiting conditions, in order to assess PGPR volatiles influence on plant response to P deficiency. This approach will use an ex-vitro co-cultivation system allowing volatiles-mediated interaction and should help us to unravel the ability of rhizobacterial volatiles to enhance plant tolerance to P deficiency. [less ▲]

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See detailQuantitative gas chromatography - mass spectrometry profiling of volatile organic compounds produced by barley (Hordeum distichon L.) roots according to plant age
Delory, Benjamin ULg; Delaplace, Pierre ULg; du Jardin, Patrick ULg et al

Poster (2014, February 07)

In chemical ecology, the roles played by root-emitted volatile organic compounds (VOCs) in biotic interactions and the quantitative analysis of such chemicals in root tissues remain poorly documented. In ... [more ▼]

In chemical ecology, the roles played by root-emitted volatile organic compounds (VOCs) in biotic interactions and the quantitative analysis of such chemicals in root tissues remain poorly documented. In this context, this study aims at developing a fully automated analytical methodology allowing both identification and accurate quantification of VOCs produced by roots of a monocotyledon plant species. Briefly, VOC emitted by crushed barley roots are successively trapped by dynamic headspace sampling on Tenax TA adsorbents, thermally desorbed and cryofocused, separated by gas chromatography (GC) and finally analysed by mass spectrometry (MS) in both SCAN and selected ion monitoring modes. Results show that barley roots mainly produce four volatile aldehydes, namely hexanal, (E)-hex-2-enal, (E)-non-2-enal and (E,Z)-nona-2,6-dienal. These molecules are well-known linoleic (C18:2) and linolenic (C18:3) acid derivatives produced via the lipoxygenase and the hydroperoxide lyase pathways of higher plants. Our findings contrast with analyses documented on aboveground barley tissues that mainly emit C6 aldehydes, alcohols and their derivative esters. Moreover, preliminary results indicate quantitative changes in the volatile profile contained in barley roots according to plant age. Multivariate statistical analyses are currently underway to quantitatively assess these changes using plants at five selected developmental stages ranging from germination to the end of tillering. [less ▲]

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See detailarchiDART: a R package allowing root system architecture analysis using Data Analysis of Root Tracings (DART) output files
Delory, Benjamin ULg; Baudson, Caroline ULg; Brostaux, Yves ULg et al

Poster (2014, February 07)

In 2010, Le Bot et al presented a free and open-access software (Data Analysis of Root Tracings - DART) allowing the analysis of complex root system architectures from captured images, particularly across ... [more ▼]

In 2010, Le Bot et al presented a free and open-access software (Data Analysis of Root Tracings - DART) allowing the analysis of complex root system architectures from captured images, particularly across time series. Using this software, a user has to manually identify roots as a set of links. After vectorization of a root system, three final data sets (RAC, TPS and LIE) can be exported as table files containing several attributes for (a) each individual root (e.g. root length), (b) each observation day or (c) each point used to construct the vectorized root system respectively. These data sets can finally be used either to calculate derived root system architecture (RSA) parameters or to draw the root system architecture at selected observation dates. However when an experiment involves the analysis and comparison of many root systems, the calculation of RSA parameters for each data set and the drawing of the corresponding vectorized root systems become time-consuming. In this context, we developed a R package, called archiDART, allowing both the automatic calculation of common root architecture parameters and the X-Y plotting of vectorized root systems for selected observation dates. [less ▲]

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See detailPerspectives offertes par la culture en association de froment et de pois protéagineux d’hiver
Pierreux, Jérome ULg; Delaplace, Pierre ULg; Roisin, Christian et al

in Autonomie protéique et biométhanisation à la ferme (2013, November 27)

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See detailEmission of sulfur-containing volatiles from Arabidopsis thaliana (L.) Heynh Col-0 related to diamondback moth (Plutella xylostella (L.)) infestation
Hien, Truong Thi Dieu ULg; Delaplace, Pierre ULg; Francis, Frédéric ULg et al

Poster (2013, June 10)

Herbivore-infested plants often release a variety of volatile organic compounds (VOCs). Here, we studied the effects of feeding Plutella xylostella (L.) (0, 3, 9, 20 pest larvae within 0-4 h and 4-8 h ... [more ▼]

Herbivore-infested plants often release a variety of volatile organic compounds (VOCs). Here, we studied the effects of feeding Plutella xylostella (L.) (0, 3, 9, 20 pest larvae within 0-4 h and 4-8 h infestation, respectively) on the emission of sulfur-containing VOCs in Arabidopsis thaliana (L.) Heynh Col-0 (A.t. Col-0) by headspace solid-phase micro-extraction coupled to gas chromatography - mass spectrometry (HS-SPME-GC/MS). The analytical results showed that the relative emission of sulfur-containing metabolites increased significantly in Arabidopsis plants subjected to P. xylostella infestation according to the density and residence duration of pest larvae on shoot organs. The main compound from infested plants was dimethyl disulfide. We suggest that the correlations between the stress level (density and time infestation) and the sulfides observed in this study provide a means to understand the changes of VOCs profile of plant under chewer infestation. [less ▲]

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See detailOptimaliser les systèmes de production des bioressources
Delaplace, Pierre ULg

Scientific conference (2013, May 07)

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See detailInfluence of rhizobacterial volatile compounds on growth and root system architecture of Brachypodium distachyon (L.) Beauv.
Mendaluk, Magdalena ULg; Varin, Sébastien; Baudson, Caroline ULg et al

Poster (2013, February 08)

Many rhizobacterial strains are classified as plant growth-promoting rhizobacteria (PGPR) and are potentially useful to enhance plant fitness and productivity. Among the mechanisms by which PGPR improve ... [more ▼]

Many rhizobacterial strains are classified as plant growth-promoting rhizobacteria (PGPR) and are potentially useful to enhance plant fitness and productivity. Among the mechanisms by which PGPR improve plant growth, the emission of volatile organic compounds (VOCs) and their biological impacts on plants remain little documented. The aim of this work is to evaluate the growth promotion ability of 19 bacterial strains on the model grass Brachypodium distachyon Bd21 (Bd21), with a focus on this peculiar mode of interaction. A collection of 19 strains was selected for their known growth promotion potential. The impact of the rhizobacteria on the model grass was studied using an in vitro cocultivation system allowing interactions through VOCs without physical contact between plants and bacteria. The results show that the VOCs of several strains promote total biomass production, modulate biomass partitioning and affect shoot and root architecture of Bd21. The effects of the PGPR strains emitting different volatile blends were investigated via principal component analysis coupled to clustering and univariate analysis to unravel their biological effects. On the basis of volatile emissions and of their effects on plant growth, two strains have been selected in order to evaluate such interactions using an ex vitro system. The set-up of this system is in progress. [less ▲]

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See detailDevelopment of an experimental device allowing plant-plant interaction studies and in situ dynamic trapping of volatile organic compounds emitted by barley (Hordeum distichon L.) roots
Delory, Benjamin ULg; Delaplace, Pierre ULg; Fauconnier, Marie-Laure ULg et al

in Communications in Agricultural and Applied Biological Sciences (2013, February 08), 78(1), 97-102

In response to wounding or herbivore attack, leaves and roots of higher plants release volatile organic compounds (VOCs). To date, VOCs analysis and plant−plant interaction studies have been mainly ... [more ▼]

In response to wounding or herbivore attack, leaves and roots of higher plants release volatile organic compounds (VOCs). To date, VOCs analysis and plant−plant interaction studies have been mainly performed on aboveground plant tissues, leaving the roles played by root VOCs in plant−plant interaction unexplored. In this context, this project aims at setting up an original experimental device allowing both dynamic trapping of VOCs emitted by mechanically damaged H. distichon roots and the study of the roles played by root VOCs in intra and interspecific plant−plant interactions. The experimental device consists of Barley seedlings cultivated in closed PTFE reactors filled with wet sand. Before being analysed by gas chromatography−mass spectrometry, root VOCs are trapped via a dynamic system on Tenax cartridges using a charcoal-filtered and humidified air. Preliminary results show that 7 day-old wounded Barley roots emit C9 fatty acid derivatives (E-non-2-enal and nona-2,6-dienal) as major compounds, contrasting with aboveground plant tissues that mainly emit C6 alcohols, aldehydes, and their derivative esters. For plant−plant interaction studies, receiver plants are exposed to an airflow enriched with VOCs from root damaged Barley plants of the same age. [less ▲]

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See detailImplication of microRNAs in the response of Brachypodium distachyon (L.) Beauv. root system architecture to rhizobacterial volatiles
Baudson, Caroline ULg; Bouché, Frédéric ULg; Saunier de Cazenave, Magdalena ULg et al

Scientific conference (2013, February)

The metabolic roles of volatile organic compounds (VOCs) emitted by plant growth-promoting rhizobacteria (PGPR) and the identity of the molecules responsible for the growth promotion are still poorly ... [more ▼]

The metabolic roles of volatile organic compounds (VOCs) emitted by plant growth-promoting rhizobacteria (PGPR) and the identity of the molecules responsible for the growth promotion are still poorly documented. As well, the implication of microRNAs in root development is a recent discovery that deserves to be explored. In this study, the implication of microRNAs in the response of Brachypodium distachyon (L.) Beauv. Bd21 root architecture to rhizobacterial VOCs was investigated. Nineteen PGPR strains were screened to select those showing the strongest phenotypic effects. The strain Bacillus subtilis AP305-GB03 induced the most important promotion of biomass production and root development. Total RNA extraction and RT-qPCR analysis of microRNAs were performed on Bd21 root samples. The expression of miR160 a-d, miR164 f, miR167 c-d, miR397 b and miR399 a-b was measured in roots every 2 days during the first 10 days of Bd21 development, in the presence or absence of the bacterial VOCs. Differences in the expression profile of miR164 f and miR167 c-d were observed in the roots exposed to GB03 VOCs, as compared to the control. These differences could be correlated to the root system architecture modifications observed after 10 days of growth with GB03. miR397 b and miR399 a-b also showed differences in the expression profile of roots exposed to the bacterial VOCs. These microRNAs have been respectively involved in cold stress tolerance and in the response to phosphate starvation. [less ▲]

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See detailNatural variation of the root morphological response to nitate supply in Arabidopsis thaliana
De Pessemier, Jérôme; Chardon, Fabien; Juraniec, Michal et al

in Mechanisms of Development (2013), 130(1), 45-53

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See detailPlant growth- promoting rhizobacteria emit volatiles compounds with biostimulation activity in dicot and monocot plant species.
du Jardin, Patrick ULg; Delaplace, Pierre ULg; Varin, Sébastien et al

in Perata, Pierdomenico; Brown, Patrick; Ponchet, Michel (Eds.) Abstracts Book for Oral and Poster Presentations of the 1st World Congress on the use of Biostimulants in Agriculture (2012, November)

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See detailImpact of Myzus persicae infestation on the volatile emission of Arabidopsis thaliana Col-0.
Hien, Truong Thi Dieu ULg; Delaplace, Pierre ULg; Francis, Frédéric ULg et al

Poster (2012, July 22)

Being members of complex communities, plants often emit a wide range of volatile organic compounds to defend themselves against insect invasions. Although many studies exist on insect-induced plant ... [more ▼]

Being members of complex communities, plants often emit a wide range of volatile organic compounds to defend themselves against insect invasions. Although many studies exist on insect-induced plant volatile emission, most of them either compare the influences of various herbivore species on one plant species or the impact of a given herbivore on several host plant species. Moreover, informations related to the influence of insect density as well as the infestation duration are still needed. Here, we showed that a sucking insect – Myzus persicae (green peach aphid) induced the volatile emission from Arabidopsis thaliana Columbia wild-type (A.thaliana Col-0) under laboratory conditions based on results obtained by solid-phase micro-extraction coupled with gas chromatography - mass spectrometry (SPME-GC/MS). The released volatile blend was discussed in relation to related biosynthesis pathways and functions. These included terpenoids, green leaf volatiles, alcohols and isothiocyanate. The qualitative and overall proportion of volatile components differed depended on the number and residence duration of aphids on leaves. By studying the effects of sucking insect stresses to plant, we not only aim to contribute to the fundamental understanding of the emission of volatile components in the interaction between plants and pests, but also to provide standardised and easy to use assays to assess A.thaliana volatile changes according to cross stresses, including both biotic and abiotic ones in ongoing experiments. [less ▲]

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