References of "Defraigne, Jean-Olivier"
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See detailNuclear Magnetic Resonance Metabolomic Profiling of Mouse Kidney, Urine and Serum Following Renal Ischemia/Reperfusion Injury.
Jouret, François ULg; Leenders, Justine ULg; Defraigne, Jean-Olivier ULg et al

in PLoS ONE (2016), 11(9), 1-14

Abstract BACKGROUND: Ischemia/reperfusion (I/R) is the most common cause of acute kidney injury (AKI). Its pathophysiology remains unclear. Metabolomics is dedicated to identify metabolites involved in ... [more ▼]

Abstract BACKGROUND: Ischemia/reperfusion (I/R) is the most common cause of acute kidney injury (AKI). Its pathophysiology remains unclear. Metabolomics is dedicated to identify metabolites involved in (patho)physiological changes of integrated living systems. Here, we performed 1H-Nuclear Magnetic Resonance metabolomics using urine, serum and kidney samples from a mouse model of renal I/R. METHODS: Renal 30-min ischemia was induced in 12-week-old C57BL/6J male mice by bilaterally clamping vascular pedicles, and was followed by 6, 24 or 48-hour reperfusion (n = 12/group). Sham-operated mice were used as controls. Statistical discriminant analyses, i.e. principal component analysis and orthogonal projections to latent structures (OPLS-DA), were performed on urine, serum and kidney lysates at each time-point. Multivariate receiver operating characteristic (ROC) curves were drawn, and sensitivity and specificity were calculated from ROC confusion matrix (with averaged class probabilities across 100 cross-validations). RESULTS: Urine OPLS-DA analysis showed a net separation between I/R and sham groups, with significant variations in levels of taurine, di- and tri-methylamine, creatine and lactate. Such changes were observed as early as 6 hours post reperfusion. Major metabolome modifications occurred at 24h post reperfusion. At this time-point, correlation coefficients between urine spectra and conventional AKI biomarkers, i.e. serum creatinine and urea levels, reached 0.94 and 0.95, respectively. The area under ROC curve at 6h, 24h and 48h post surgery were 0.73, 0.98 and 0.97, respectively. Similar discriminations were found in kidney samples, with changes in levels of lactate, fatty acids, choline and taurine. By contrast, serum OPLS-DA analysis could not discriminate sham-operated from I/R-exposed animals. CONCLUSIONS: Our study demonstrates that renal I/R in mouse causes early and sustained metabolomic changes in urine and kidney composition. The most implicated pathways at 6h and 24h post reperfusion include gluconeogenesis, taurine and hypotaurine metabolism, whereas protein biosynthesis, glycolysis, and galactose and arginine metabolism are key at 48h post reperfusion. [less ▲]

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See detailEffect of Ribes nigrum leaf extracts on endothelium-dependent vasorelaxation
Tabart, Jessica; Shini-Kerth, Valérie; PINCEMAIL, Joël ULg et al

Poster (2016, April 22)

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See detailExtending abdominal aortic aneurysm detection to older age groups: preliminary results from the Liege screening programme.
MAKRYGIANNIS, Georgios ULg; Labalue, Philippe; Erpicum, Marie ULg et al

in Annals of Vascular Surgery (2016)

BACKGROUND: There is evident benefit in terms of reduced aneurysm related mortality from screening programmes of abdominal aortic aneurysm (AAA) in men aged 65 and more. Recent studies in UK and Sweden ... [more ▼]

BACKGROUND: There is evident benefit in terms of reduced aneurysm related mortality from screening programmes of abdominal aortic aneurysm (AAA) in men aged 65 and more. Recent studies in UK and Sweden have shown a decline of the prevalence of AAA in the general population. Current screening policies (e.g. men aged 65-74 years) however do not account for ageing and increased life expectancy of western populations. This study investigated AAA detection by extending the target population to older age groups (75-85 years). METHODS: AAA screening was conducted in the County of Chaudfontaine (Liege, Belgium) on the population of elderly (N=3,054). The participation rate was 36%. The 1,101 participants (722 men aged 65-85 and 379 women aged 74-85 years) were examined by ultrasound (US) scan. AAA was defined as an infrarenal aortic outer-outer diameter of at least 3 cm. Demographics, clinical parameters and risk factors were also recorded. AAA prevalence was estimated and patients with and without AAA were compared by logistic regression. RESULTS: The overall AAA prevalence was 3.6% (n=40). In female participants, AAA prevalence was low (1.3%). In men, it amounted 2.7% in the 65-74 age group but rose to 7.3% in the age-extended group (75-85 years). Further in addition to age, height, current smoking, history of coronary artery disease, hypercholesterolemia, peripheral artery disease of the lower limbs and varicose veins were significantly associated with the presence of AAA. CONCLUSION: These preliminary findings, based on a representative sample of the elderly population of the Liege region, support the idea that current AAA screening policies should be updated to cover an increasingly ageing population. The presence of varicose veins as a potential risk factor for AAA should also be considered during screening. [less ▲]

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See detailThe leaf extract of Ribes nigrum L. is a potent stimulator of the endothelial formation of NO in cultured endothelial cells and porcine coronary artery rings
Tabart, Jessica; Schini-Kerth, Valérie; PINCEMAIL, Joël ULg et al

in Journal of Berry Research (2016), 6

BACKGROUND: Endothelial dysfunction is a major hallmark of most types of cardiovascular diseases. Numerous plant extracts have been shown to cause endothelium-dependent relaxations by increasing the ... [more ▼]

BACKGROUND: Endothelial dysfunction is a major hallmark of most types of cardiovascular diseases. Numerous plant extracts have been shown to cause endothelium-dependent relaxations by increasing the endothelial formation of the potent vasoprotective factor, nitric oxide (NO). OBJECTIVE: The ability of different Ribes nigrum L. extracts (Grossulariaceae) to induce endothelium-dependent relaxation by stimulating the endothelial formation of NO was assesssed. METHODS: Ribes nigrum extracts were prepared from buds, berries and leaves by extraction (Acetone:H2O:Acetic Acid; 70/28/2 (v/v/v)) and lyophilized after acetone evaporation. The ability of the extracts to stimulate the endothelial formation of NO was assessed using cultured endothelial cells and isolated porcine coronary artery rings. RESULTS: The Ribes nigrum leaf extract increased to a greater extent than the bud and the berry extracts the formation of NO, and up-regulated eNOS mRNA expression in cultured endothelial cells (the stimulatory effects amounted to 197 ± 9 %, 134 ± 6 % and 118 ± 5 %, respectively). The leaf extract induced greater relaxations of isolated coronary arteries with endothelium than the bud and the berry extracts whereas no such effects were observed in rings without endothelium. Relaxations to the leaf extract were minimally affected by indomethacin and by inhibitors of endothelium-dependent hyperpolarization response, and markedly reduced by NG-nitro-L-arginine. CONCLUSIONS: The present findings indicate that the Ribes nigrum leaf extract is a more potent inducer of the endothelial formation of NO than the bud and the berry extracts. [less ▲]

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See detailIgG4-related disease causing rapid evolution of a severe aortic valvular stenosis
BRULS, Samuel ULg; Courtois, Audrey ULg; DELVENNE, Philippe ULg et al

in The Annals of Thoracic Surgery (2016)

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See detailMultimodality imaging assessment of the deleterious role of the intraluminal thrombus on the growth of abdominal aortic aneurysm in a rat model
NCHIMI LONGANG, Alain ULg; Courtois, Audrey ULg; EL HACHEMI, Mounia ULg et al

in European Radiology (2016), 26

Objectives To evaluate imaging changes occurring in a rat model of elastase-induced abdominal aortic aneurysm (AAA), with emphasis on the intraluminal thrombus (ILT) occurrence. Methods The post-induction ... [more ▼]

Objectives To evaluate imaging changes occurring in a rat model of elastase-induced abdominal aortic aneurysm (AAA), with emphasis on the intraluminal thrombus (ILT) occurrence. Methods The post-induction growth of the AAA diameter was characterized using ultrasound in 22 rats. ILT was reported on 13 rats that underwent 14 magnetic resonance imaging (MRI) 2-18 days post-surgery, and on 10 rats that underwent 18 fluoro-deoxyglucose (FDG) positron emission tomography (PET)/microcomputed tomography examinations 2-27 days post-surgery. Logistic regressions were used to establish the evolution with time of AAA length, diameter, ILT thickness, volume, stratification, MRI and FDG PET signalling properties, and histological assessment of inflammatory infiltrates. Results All of the following significantly increased with time post-induction (p < 0.001): AAA length, AAA diameter, ILT maximal thickness, ILT volume, ILT iron content and related MRI signalling changes, quantitative uptake on FDG PET, and the magnitude of inflammatory infiltrates on histology. However, the aneurysm growth peak followed occurrence of ILT approximately 6 days after elastase infusion. Conclusion Our model emphasizes that occurrence of ILT precedes AAA peak growth. Aneurysm growth is associated with increasing levels of iron, signalling properties changes in both MRI and FDG PET, relating to its biological activities. [less ▲]

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See detailPrevention of Incisional Hernias by Prophylactic Mesh-augmented Reinforcement of Midline Laparotomies for Abdominal Aortic Aneurysm Treatment: A Randomized Controlled Trial.
Muysoms, Filip E.; DETRY, Olivier ULg; Vierendeels, Tijl et al

in Annals of surgery (2016), 263(4), 638-645

BACKGROUND: The incidence of incisional hernias after abdominal aortic aneurysm repair is high. Prophylactic mesh-augmented reinforcement during laparotomy closure has been proposed in patients at high ... [more ▼]

BACKGROUND: The incidence of incisional hernias after abdominal aortic aneurysm repair is high. Prophylactic mesh-augmented reinforcement during laparotomy closure has been proposed in patients at high risk of incisional hernia. METHODS: A multicenter randomized trial was conducted on patients undergoing elective abdominal aortic aneurysm repair through a midline laparotomy (Clinical.Trials.gov: NCT00757133). In the study group, retromuscular mesh-augmented reinforcement was performed with a large-pore polypropylene mesh (Ultrapro, width 7.5 cm). The primary endpoint was the incidence of incisional hernias at 2-year follow-up. RESULTS: Between February 2009 and January 2013, 120 patients were recruited at 8 Belgian centers. Patients' characteristics at baseline were similar between groups. Operative and postoperative characteristics showed no difference in morbidity or mortality. The cumulative incidence of incisional hernias at 2-year follow-up after conventional closure was 28% (95% confidence interval [CI], 17%-41%) versus 0% (95% CI, 0%-6%) after mesh-augmented reinforcement (P < 0.0001; Fisher exact test). The estimated "freedom of incisional hernia" curves (Kaplan-Meier estimate) were significantly different across study arms (chi = 19.5, P < 0.0001; Mantel-Cox test). No adverse effect related to mesh-augmented reinforcement was observed, apart from an increased mean time to close the abdominal wall for mesh-augmented reinforcement compared with the control group: 46 minutes (SD, 18.6) versus 30 minutes (SD, 18.5), respectively (P < 0.001; Mann-Whitney U test). CONCLUSIONS: Prophylactic retromuscular mesh-augmented reinforcement of a midline laparotomy in patients with abdominal aortic aneurysm is safe and effectively prevents the development of incisional hernia during 2 years, with an additional mean operative time of 16 minutes. [less ▲]

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See detailHigh reactive oxygen species in fibrotic and non-fibrotic skin of Patients with diffuse cutaneous systemic sclerosis
BOURJI, Khalil; MEYER, Alain; CHATELUS, Emmanuel et al

in Free Radical Biology & Medicine (2015), 87

Systemic Sclerosis (SSc) is a chronic multisystemic connective tissue disease characterized by progressive fibrosis affecting skin and internal organs. Despite serious efforts to unveil the pathogenic ... [more ▼]

Systemic Sclerosis (SSc) is a chronic multisystemic connective tissue disease characterized by progressive fibrosis affecting skin and internal organs. Despite serious efforts to unveil the pathogenic mechanisms of SSc, they are still unclear. High levels of Reactive Oxygen Species (ROS) in affected patients have been shown, and ROS are suggested to play a role in fibrosis pathogenesis. In this study we evaluate ROS levels in non-fibrotic and fibrotic skin of patients with SSc and we compare them with those obtained from healthy controls. Patients and Methods We enrolled 9 SSc patients fulfilling the EULAR/ACR classification criteria and 7 healthy controls. Patients included were 4 men and 5 women with mean age of 46 ±10 yrs. Controls were matched by sex and age. All patients were affected by diffuse cutaneous form of SSc and the ANA pattern anti-Scl70. Mean disease duration was 7.5±5 yrs. Skin involvement was evaluated by modified Rodnan Skin Score (mRSS). Skin samples (4 mm punch biopsy) were taken from fibrotic skin and non-fibrotic skin of patients and from healthy controls as well. To detect ROS, specimens were analyzed immediately after sampling by electron paramagnetic resonance spectroscopy. Blood samples have been drawn from all patients and controls to assess oxidative stress biomarkers. Results ROS levels (expressed as median and range, unit of measurement was nmol/l/min/mg of dry weight) were 24.7 (10.9– 47.0) in fibrotic skin, 18.7 (7.3–34.0) in non-fibrotic skin and 7.7 (3.5–13.6) in healthy controls skin. ROS levels in Fibrotic and Non-fibrotic skin of SSc patients were significantly higher than in Healthy Controls (p=0.002 and p=0.009, respectively). ROS levels in fibrotic skin were raised in comparison to non-fibrotic skin, when samples related to each patient were compared (p=0.01). ROS levels in fibrotic skin were correlated with forced vital capacity (r= -0.75, p=0.02) and erythrocyte sedimentation rate (r=0.70, p=0.04). All other clinical and lab parameters showed no significant correlation. When compared to controls, blood from SSc patients showed lower ascorbate (vitamin C) levels (8 [3.8-9.8] vs. 10.5 [9-19.1] mg/L, p=0.004) and higher lipid peroxides (873.5 [342-1973] vs. 422 [105-576] μmol/L, p=0.004). Conclusion Our results indicate the presence of high oxidative stress both in non-fibrotic skin and fibrotic skin of SSc patients, but with higher tendency in the latter. Raised ROS levels in non-fibrotic skin of SSc patients might be a hint of early involvement in skin fibrogenesis. However, a longitudinal prospective study is necessary for such proof. [less ▲]

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See detailRupture post-traumatique de l’isthme aortique : perspectives modernes de prise en charge
BRULS, Samuel ULg; GOFFIN, Pierre ULg; Sakalihasan, Natzi ULg et al

in Revue Médicale de Liège (2015), 70(9), 415-22

Traumatic aortic rupture of the thoracic aorta (usually at the isthmus) is frequently associated with concomitant life-threatening injuries. Historically, the conventional care consisted of surgical ... [more ▼]

Traumatic aortic rupture of the thoracic aorta (usually at the isthmus) is frequently associated with concomitant life-threatening injuries. Historically, the conventional care consisted of surgical repair of the lesion performed as soon as possible. However, in spite of constant technical improvements the morbi-mortality remains high because of these associated lesions. In addition, their management can have priority and delay aortic surgery. The endovascular approach has been shown to be a feasible and efficient technique and currently represents a valuable alternative to open surgery for patients with multiple traumas. We report a patient presenting with a traumatic aortic rupture of the aortic isthmus, which was successfully treated by delayed combined endovascular (thoracic aortic stentgrafting) and open approach (hemi-aortic arch debranching). [less ▲]

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See detailOxidative stress or not in healthy older subjects?
PINCEMAIL, Joël ULg; CHRISTELBACH, Sophie ULg; RICOUR, Céline ULg et al

in OCC2015 (2015, June)

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See detailRenal ischemia/reperfusion decreases the expression of DPP-4
Rowart, Pascal ULg; ERPICUM, Pauline ULg; Defraigne, Jean-Olivier ULg et al

Conference (2015, April 24)

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See detailMolding thrombus of an ECMO cannula floating in the right atrium.
MORIMONT, Philippe ULg; LAMBERMONT, Bernard ULg; GARSPARD, Valérie ULg et al

in Intensive Care Medicine (2015)

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See detailEtude GEROX : Evaluation du statut de stress oxydant
CHRISTELBACH, SOPHIE; RICOUR, Céline ULg; Dardenne, Nadia ULg et al

Poster (2015, March)

Detailed reference viewed: 49 (9 ULg)