References of "Decuypere, Eddy"
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See detailEmbryonic Protein Undernutrition by Albumen Removal Programs the Hepatic Amino Acid and Glucose Metabolism during the Perinatal Period in an Avian Model.
Willems, Els; Hu, Tjing-Tjing; Soler Vasco, Laura et al

in PloS one (2014), 9(4), 94902

Different animal models have been used to study the effects of prenatal protein undernutrition and the mechanisms by which these occur. In mammals, the maternal diet is manipulated, exerting both direct ... [more ▼]

Different animal models have been used to study the effects of prenatal protein undernutrition and the mechanisms by which these occur. In mammals, the maternal diet is manipulated, exerting both direct nutritional and indirect hormonal effects. Chicken embryos develop independent from the hen in the egg. Therefore, in the chicken, the direct effects of protein deficiency by albumen removal early during incubation can be examined. Prenatal protein undernutrition was established in layer-type eggs by the partial replacement of albumen by saline at embryonic day 1 (albumen-deprived group), compared to a mock-treated sham and a non-treated control group. At hatch, survival of the albumen-deprived group was lower compared to the control and sham group due to increased early mortality by the manipulation. No treatment differences in yolk-free body weight or yolk weight could be detected. The water content of the yolk was reduced, whereas the water content of the carcass was increased in the albumen-deprived group, compared to the control group, indicating less uptake of nutrients from the yolk. At embryonic day 16, 20 and at hatch, plasma triiodothyronine (T3), corticosterone, lactate or glucose concentrations and hepatic glycogen content were not affected by treatment. At embryonic day 20, the plasma thyroxine (T4) concentrations of the albumen-deprived embryos was reduced compared to the control group, indicating a decreased metabolic rate. Screening for differential protein expression in the liver at hatch using two-dimensional difference gel electrophoresis revealed not only changed abundance of proteins important for amino acid metabolism, but also of enzymes related to energy and glucose metabolism. Interestingly, GLUT1, a glucose transporter, and PCK2 and FBP1, two out of three regulatory enzymes of the gluconeogenesis were dysregulated. No parallel differences in gene expressions causing the differences in protein abundance could be detected pointing to post-transcriptional or post-translational regulation of the observed differences. [less ▲]

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See detailThe endocrine control of energy homeostasis in chickens
Decuypere, Eddy; Everaert, Nadia ULg; Song, Zhigang et al

in General and Comparative Endocrinology (2013)

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See detailMaternal transition of prenatal undernutrition in the laying hen
Willems, Els; Wang, Yufeng; Koppenol, Astrid et al

Poster (2013)

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See detailThe endocrine control of energy homeostasis in chickens.
Song, Zhigang; Everaert, Nadia ULg; Wang, Yufeng et al

in General and comparative endocrinology (2013), 190

Energy homeostasis (balance) depends on the relationship between the amount of consumed feed energy and energy expenditure. Coordination of energy expenditure and feed intake (appetite) is necessary for ... [more ▼]

Energy homeostasis (balance) depends on the relationship between the amount of consumed feed energy and energy expenditure. Coordination of energy expenditure and feed intake (appetite) is necessary for the regulation of body composition. The hypothalamus integrates peripheral and central signals to generate satiety or hunger. Birds and mammals utilize common signaling molecules but some molecules possess different/opposite functions. If relevant, particular differences with the mammalian regulatory system are highlighted in this review. For example, obestatin had no significant effect on feed intake of chicks, but it was claimed to decrease food intake in mammalian species. Ghrelin displayed appetite-stimulating effects in mammals but appetite-decreasing effects in birds. Recently, the function of the hypothalamic AMPK signaling pathway on feed intake regulation has received considerable attention in poultry. Alpha-lipoic acid might exert its appetite-decreasing effect by the AMPK signaling pathway. This review discusses the central regulation of energy homeostasis, role of (an)orexigenic peptides, effect of feed deprivation on hypothalamic neuropeptide gene expression and provides a model for involvement of AMPK in the regulation of avian energy balance. [less ▲]

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See detailThe effect of albumen removal before incubation (embryonic protein under-nutrition) on the post-hatch performance, regulators of protein translation activation and proteolysis in neonatal broilers.
Everaert, Nadia ULg; Metayer-Coustard, Sonia; Willemsen, Hilke et al

in The British journal of nutrition (2013), 110(2), 265-74

Albumen was removed from broiler eggs before the start of incubation to induce prenatal protein under-nutrition in chicken embryos. With this method, the direct effect of protein deficiency was ... [more ▼]

Albumen was removed from broiler eggs before the start of incubation to induce prenatal protein under-nutrition in chicken embryos. With this method, the direct effect of protein deficiency was investigated, differing from mammalian models manipulating the maternal diet where indirect, hormonal effects can interfere. Based on the estimated albumen/egg weight ratio, 10 % of albumen was removed with an 18G needle, after making a hole at the sharp end of the egg with another 18G needle. Eggs were taped thereafter. The sham group underwent the same procedure, except that no albumen was removed. Control eggs did not receive any treatment. The removal of albumen decreased both embryonic and post-hatch body weight up to day 7 compared with the control group. On embryonic day 18, embryos from the albumen-deprived group had higher plasma uric acid levels compared with the sham (P= 0.016) and control (P= 0.009) groups. Moreover, a lower plasma amino acid concentration was observed at hatch compared with the sham (P= 0.038) and control (P= 0.152) groups. These findings indicate an altered protein metabolism. At hatch, a higher mRNA expression of muscle ring finger-1 (MuRF1), a gene related to proteolysis, was observed in albumen-deprived chicks compared with the control and sham chicks, together with an up-regulated expression of atrogin-1 (another atrogene) at this time point in the male protein-deficient chicks. These findings suggest that muscle proteolysis is transiently increased by the removal of albumen before the start of incubation. No evidence was found for altered protein synthesis capacity and translational efficiency in albumen-deprived chicks. [less ▲]

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See detailPrenatal undernutrition of the chicken changes the glucose metabolism in the liver at hatch and alters post-hatch performance
Willems, Els; Hu, T; Buyse, Johan et al

in Proceedings of the XXIV World's Poultry Congress (2012)

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See detailThe Winner of the Incubation Became the Loser in Post-hatch Performance
Wang, Yufeng; Li, Yue; Willemsen, Hilke et al

in Proceedings of the XXIV World's Poultry Congress (2012)

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See detailInvestigation of the glucose metabolism of the chicken by embryonic injection of tolbutamide
Franssens, Lies; Wang, Yufeng; Willems, Els et al

in Proceedings of the XXIV World's Poultry Congress (2012)

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See detailPrenatal undernutrition of the chicken embryo leads to changes in plasma T3 and corticosterone levels
Willems, Els; Wang, Yufeng; Willemsen, Hilke et al

Poster (2012)

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See detailEndocrine factors and AMPKα1 are involved in the spread of hatch and subsequent neonatal performance of broiler chicks
Wang, Yufeng; Li, Yue; Willems, Els et al

Poster (2012)

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See detailThe importance of albumen-proteins during embryonic development of layer-type chickens on quality of eggs laying in adult life
Willems, Els; Wang, Yufeng; Willemsen, Hilke et al

Scientific conference (2012)

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See detailInvestigation of the glucose metabolisme of the chicken embryo by injection of insulin
Franssens, Lies; Koppenol, Astrid; Wang, Yufeng et al

Poster (2012)

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See detailPeripheral "chicken" obestatin administration does not affect feed intake and gut muscle contractility of meat-type and layer-type chicks (Gallus gallus domesticus).
Song, Zhigang; Verhulst, Pieter-Jan; Ansari, Zarbakht et al

in Regulatory peptides (2012), 177(1-3), 60-7

Obestatin has recently been discovered in the rat stomach. As for ghrelin, the 23-amino acid obestatin is also derived from post-translational processing of the prepro-ghrelin gene but seems to have ... [more ▼]

Obestatin has recently been discovered in the rat stomach. As for ghrelin, the 23-amino acid obestatin is also derived from post-translational processing of the prepro-ghrelin gene but seems to have opposite effects on feed intake. In avian species, ghrelin is mainly present in the proventriculus and decreases feed intake, as opposed to its orexigenic properties in mammals. An obestatin-like sequence was also found in the avian ghrelin precursor protein but the potential involvement of this peptide in appetite regulation of chickens is unclear. We therefore investigated the effects of a single peripheral administration of this predicted "chicken" obestatin peptide on voluntary feed intake of 7- to 9-day-old meat-type and layer-type chicks. "Chicken" obestatin was injected intraperitoneally or intravenously at a dose of 1 nmol or 10 nmol/100 g body weight and feed intake was measured up to 4 h post injection. None of these treatments did reveal any effect of the putative "chicken" obestatin on appetite of either meat-type of layer-type chicks. Furthermore, "chicken" obestatin also failed to affect the in vitro contractility of muscle strips from crop and proventriculus. In conclusion, in the given experimental settings, the putative "chicken" obestatin has indistinctive physiological effects on feed intake and in vitro muscle contractility of gut segments, and hence its functional properties in ingestive behavior of avian species remain obscure. [less ▲]

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See detailFasting alters protein expression of AMP-activated protein kinase in the hypothalamus of broiler chicks (Gallus gallus domesticus).
Song, Zhigang; Liu, Lei; Yue, Yunshuang et al

in General and comparative endocrinology (2012), 178(3), 546-55

An experiment was conducted to investigate the effects of fasting and re-feeding on hypothalamic 5'-AMP-activated protein kinase (AMPK) levels and (an)orexigenic neuropeptides. Male Arbor Acres chicks (7 ... [more ▼]

An experiment was conducted to investigate the effects of fasting and re-feeding on hypothalamic 5'-AMP-activated protein kinase (AMPK) levels and (an)orexigenic neuropeptides. Male Arbor Acres chicks (7-day-old, n=160) were allocated to four equal treatment groups: control chicks (fed ad libitum for 48 h, C48), chicks that were fasted for 48 h (F48), chicks that were first fasted for 48 h and then re-fed for 24h (F48C24), and chicks that were fed ad libitum for 72h (C72). Fasting for 48 h significantly (P<0.05) increased the ratio of phosphorylated AMPKalpha to total AMPKalpha and phosphorylated LKB1 to total LKB1, whereas re-feeding for 24h reduced these ratios to that of the ad libitum fed C72 chicks. The gene expressions of agouti-related peptide (AgRP), neuropeptide Y (NPY), melanocortin receptor 4, melanin-concentrating hormone, prepro-orexins and carnitine palmitoyltransferase-1 were significantly (P<0.05) increased in the fasted chicks relative to the ad libitum fed C48 group. The gene expression of pro-opiomelanocortin (POMC), as well as cocaine- and amphetamine-regulated transcript (CART) was not affected by the nutritional status. Fasting significantly (P<0.05) decreased the mRNA levels of fatty acid synthase (FAS) and sterol regulatory element binding protein-1 (SREBP-1). The results suggest that the LKB1/AMPK signal pathway is involved in the energy homeostasis of fasted chicks, and its possible role in feed intake regulation might be mediated by the AgRP/NPY rather than the POMC/CART pathway. [less ▲]

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See detailEarly-age feed restriction affects viability and gene expression of satellite cells isolated from the gastrocnemius muscle of broiler chicks.
Li, Yue; Yang, Xiaojing; Ni, Yingdong et al

in Journal of animal science and biotechnology (2012), 3(1), 33

BACKGROUND: Muscle growth depends on the fusion of proliferate satellite cells to existing myofibers. We reported previously that 0-14 day intermittent feeding led to persistent retardation in myofiber ... [more ▼]

BACKGROUND: Muscle growth depends on the fusion of proliferate satellite cells to existing myofibers. We reported previously that 0-14 day intermittent feeding led to persistent retardation in myofiber hypertrophy. However, how satellite cells respond to such nutritional insult has not been adequately elucidated. RESULTS: One-day-old broiler chicks were allocated to control (Con, ad libitum feeding), intermittent feeding (IF, feed provided on alternate days) and re-feeding (RF, 2 days ad libitum feeding after 12 days of intermittent feeding) groups. Chickens were killed on Day 15 and satellite cells were isolated. When cultured, satellite cells from the IF group demonstrated significant retardation in proliferation and differentiation potential, while RF partly restored the proliferation rate and differentiation potential of the satellite cells. Significant up-regulation of insulin like growth factor I receptor (IGF-IR) (P<0.05) and thyroid hormone receptor alpha (TRalpha) (P<0.05), and down-regulation of growth hormone receptor (GHR) (P<0.01) and IGF-I (P<0.01) mRNA expression was observed in freshly isolated IF satellite cells when compared with Con cells. In RF cells, the mRNA expression of IGF-I was higher (P<0.05) and of TRalpha was lower (P<0.01) than in IF cells, suggesting that RF restored the mRNA expression of TRalpha and IGF-I, but not of GHR and IGF-IR. The Bax/Bcl-2 ratio tended to increase in the IF group, which was reversed in the RF group (P<0.05), indicating that RF reduced the pro-apoptotic influence of IF. Moreover, no significant effect of T3 was detected on cell survival in IF cells compared with Con (P<0.001) or RF (P<0.05) cells. CONCLUSIONS: These data suggest that early-age feed restriction inhibits the proliferation and differentiation of satellite cells, induces changes in mRNA expression of the GH/IGF-I and thyroid hormone receptors in satellite cells, as well as blunted sensitivity of satellite cells to T3, and that RF partially reverses these effects. Thus, a moderate nutritional strategy for feed restriction should be chosen in early chick rearing systems. [less ▲]

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See detailEffects of Spread of Hatch and Delayed Feed Access on Broiler Post-Hatch Performance up to Day 5
Wang, Yufeng; Li, Yue; Willems, Els et al

in Proceedings of Fundamental Physiology of the European working group of physiology and perinatal development in poultry. (2011)

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See detailThe effects of egg position during the last three days of incubation on the hatching process and perinatal adaptation
Kamers, Bram; Beckers, Steven; Willemsen, Hilke et al

in The 5th combined workshop: Fundamental Physiology of the European working group of physiology and perinatal development in poultry (2011)

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See detailThe effect of insulin injection on the glucose metabolism of the chicken embryo
Franssens, Lies; Willems, Els; Willemsen, Hilke et al

Poster (2011)

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See detailEffect of prenatal undernutrition of the chicken embryo on postnatal performance up to day 21
Willems, Els; Willemsen, Hilke; Wang, Yufeng et al

in The 5th combined workshop: Fundamental Physiology of the European working group of physiology and perinatal development in poultry (2011)

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See detailEffect of prenatal undernutrition of the chicken embryo on growth and metabolism up to day 7
Willems, Els; Willemsen, Hilke; Li, Yue et al

Poster (2011)

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