Radioimmunoassay of bovine placental lactogen using recombinant and native preparations: determination of fetal concentrations across gestation; Melo de Sousa, Noelita ; Hornick, Jean-Luc et alin Reproduction, Fertility, & Development (2007), 19(7), 877-885 Concentrations of bovine placental lactogen ( bPL) were determined in fetal plasma samples by twelve double-antibody competitive radioimmunoassay systems ( RIA I - XII) based on either recombinant bPL ... [more ▼] Concentrations of bovine placental lactogen ( bPL) were determined in fetal plasma samples by twelve double-antibody competitive radioimmunoassay systems ( RIA I - XII) based on either recombinant bPL ( non- glycosylated) or native bPL ( glycosylated). Both preparations were used as standard and tracer, and for primary antisera production. The minimum detection limit measured by these RIA varied from 0.02 to 0.6 ng bPLmL(-1). The coefficients of correlation of different bPL RIA systems were up to 90% ( P< 0.0001) when each RIA was tested against the average values of all twelve RIA systems. All developed RIA were used to investigate the incidence of different bPL isoforms in bovine fetal serum samples ( n= 71). Fetal concentrations ranged from 11.8 to 35.7 ng mL(-1) at the third month and from 1.1 to 13.5 ngmL(-1) at the ninth month of gestation. They tended to decrease with advancing gestation. In general, those RIA systems that used recombinant bPL as the standard measured higher values than those using the native bPL preparation. These differences decreased toward the end of gestation ( P< 0.05), suggesting a lower rate of glycosylation. Our results provide evidence of different glycosylated isoforms of bPL in fetal serum at different gestation periods. [less ▲] Detailed reference viewed: 25 (5 ULg) A comparison of somatic cell count and antimicrobial susceptibility of subclinical mastitis pathogens in organic and conventional dairy herds; Detilleux, Johann ; Motkin, Michel et alin Annales de Médecine Vétérinaire (2005), 149(3), 173-182 A comparison of somatic cell count and antimicrobial susceptibility of subclinical mastitis pathogens in organic and conventional dairy herds.Bovine subclinical mastitis is the most important disease ... [more ▼] A comparison of somatic cell count and antimicrobial susceptibility of subclinical mastitis pathogens in organic and conventional dairy herds.Bovine subclinical mastitis is the most important disease affecting dairy cows. The fluctuating increase in somatic cell count (SCC) that occurs causes major economic losses in dairy industry. This comparative study between conventional and organic dairy herds was conducted in the aim to better characterize which consequences might have different management practices on SCC but also on the frequency of pathogens isolated and their antimicrobial susceptibility. Four conventional and four organic herds, with bulk milk SCC >300x103cells/ml were selected, in which respectively 47 and 44 cows were investigated. Each quarter was sampled 3 times at 15 days interval for SCC, microbiological analysis and antimicrobial susceptibility. In both herd categories, major pathogens isolated were by order of importance Streptococcus uberis, Staphylococcus aureus and Streptococcus dysgalactiae with a great impact on SCC. Coagulase negative staphylococci were the most frequent minor germs and had a moderated but real impact on SCC. In certified organic dairy farms, the three most frequently isolated major pathogens were significantly more susceptible to antimicrobials in vitro. This study suggests that the limited use of antibiotics in organic dairy herds could explain, at least in part, the lower resistance obtained from analysed isolates. [less ▲] Detailed reference viewed: 78 (12 ULg) Comparaison du taux cellulaire et de la sensibilité antimocrobienne des germes responsables de mammites subcliniques entre les filières conventionnelles et biologique; Detilleux, Johann ; et alin Annales de Médecine Vétérinaire (2005), 149 Detailed reference viewed: 18 (2 ULg) Etude sur les fluctuations de température relevées dans 25 compartiments de maternité et de post-sevrageNicks, Baudouin ; ; et alin 24èmes Journées de la Recherche Porcine en France (1992) Temp. was measured for 7 days, every 20 min., in 20 farrowing houses and 5 weaning houses. The mean stocking rate in the farrowing houses was 70% and the air space per litter (1 sow and 8 piglets) 25 m ... [more ▼] Temp. was measured for 7 days, every 20 min., in 20 farrowing houses and 5 weaning houses. The mean stocking rate in the farrowing houses was 70% and the air space per litter (1 sow and 8 piglets) 25 m. In the weaning houses the mean stocking density was 84% and the air space per piglet 1.3 m. Four different heating systems were used: radiators, floor heating, gas heaters and aerotherms. Artificial ventilation was provided in 88% of the compartments and natural ventilation in the others. The mean inside and outside temp. were 22 2 and 106C resp. A good estimate of the mean daily temp. was provided with only the min. and max. daily records. No more than one measurement per h was needed to assess the daily variations. The mean daily temp. range inside the buildings was 33C, ranging from 1 to 11C. The largest inside temp. variations were observed with high and fluctuating outside temp. On an hourly, daily or weekly basis, the outside temp. range was reduced by two thirds. This value can be used as a reference to judge the ability of the building and its equipment to maintain a constant inside temp. [less ▲] Detailed reference viewed: 18 (0 ULg) Caractéristiques hygrométriques et niveau de pollution de l'air dans 9 locaux en porcherie d'élevage; Nicks, Baudouin ; et alin Annales de Médecine Vétérinaire (1991), 135 Detailed reference viewed: 9 (1 ULg) Etude sur les fluctuations de température relevées dans 5 locaux de post-sevrage en porcheries; Nicks, Baudouin ; et alin Annales de Médecine Vétérinaire (1991), 135(7), 511-515 Temperature was measured during 7 days, every 20 minutes, in 5 rooms of 3 weaning houses. All the rooms were artificially ventilated but they differed by their stocking rate which ranged from 0.5 to 2.3 ... [more ▼] Temperature was measured during 7 days, every 20 minutes, in 5 rooms of 3 weaning houses. All the rooms were artificially ventilated but they differed by their stocking rate which ranged from 0.5 to 2.3 m3 per animal. The mean inside and outside temperatures were 23 +/- 3-degrees-C and 13 +/- 8-degrees-C. The outside temperature range was reduced, on average, by 60% either on a daily or a weekly basis. In terms of absolute values, the daily range varied from 2 to 80-degrees-C according to the room. An accurate estimate of the mean daily temperatures was already provided by just the minimum and maximum records. Only one measurement per hour was needed to assess the daily variations. Advices related to the stability of temperature in the rooms have to be expressed in relative terms rather than in absolute values. A 60% reduction of the outside temperature range can be suggested as a reference. [less ▲] Detailed reference viewed: 9 (0 ULg) Climatic environment and air quality in breeding pig houses; Nicks, Baudouin ; et alin proceedings of the annual conference of the Association of Veterinary Teachers and Research Workers (1991) Detailed reference viewed: 3 (0 ULg) Prévention des maladies respiratoires du porc par le contrôle du microclimat et du niveau de pollution de l'air des bâtiments.Nicks, Baudouin ; ; Vandenheede, Marc ![]() Conference (1991) Detailed reference viewed: 14 (0 ULg) Etude du microclimat et de la ventilation d'une porcherie d'engraissementNicks, Baudouin ; ; et alin 23èmes Journées de la Recherche Porcine en France (1991) Temperature, relative humidity and air speed were measured during 4 weeks in two compartments of a fattening pig house. Temperature was controlled by automatic regulation of the extractor fan. Average NH3 ... [more ▼] Temperature, relative humidity and air speed were measured during 4 weeks in two compartments of a fattening pig house. Temperature was controlled by automatic regulation of the extractor fan. Average NH3 and CO2 concentrations over a 8-h period were measured 4 times in each compartment at 1-week intervals. In a compartment without pigs, ventilation rate was measured at 8 fan speeds and air speed was measured in 6 pens. Mean temperature was 24C and relative humidity 71%. despite large variations of outside temperature, 95% of the daily variations of inside temperature were <6C and 68% of the hourly variations were <0.5C. More than 90% of the daily variations of relative humidity were <20%. Thermal regulation with the fan was therefore effective. Mean NH3 concentration was higher than the usual recommendations, 26(vs 20) ppm and the CO2 concentration lower, 1688 (vs 3000) ppm. Measurements of ventilation rate showed that the air inlet area was too small for the maximum power of the fan. The ventilation rate was at most 3200 m/h for 78 pigs. Air speeds higher than 0.2 m/s were also measured 0.5 m above the floor in some of the 6 pens. These excessive values could be related to air inlet area. [less ▲] Detailed reference viewed: 8 (0 ULg) Etude sur les fluctuations de température relevées dans 20 compartiments de maternité en porcherie d'élevage; Nicks, Baudouin ; et alin Annales de Médecine Vétérinaire (1991) Detailed reference viewed: 5 (0 ULg) Comparaison du comportement de repos de génisses sous deux régimes d'éclairementNicks, Baudouin ; ; et alin Cahiers d'Ethologie Appliquée (1989), 9 Detailed reference viewed: 8 (6 ULg) |
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