  Selected Protein Monitoring in Histological Sections by Targeted MALDI-FTICR in-source decay Imaging.Calligaris, David  ; Longuespée, Rémi ; Debois, Delphine et alin Analytical Chemistry (2013), sous presse MALDI mass spectrometry imaging (MALDI MSI) is a rapidly growing method in biomedical research allowing molecular mapping of proteins on histological sections. The images can be analyzed in terms of ... [more ▼] MALDI mass spectrometry imaging (MALDI MSI) is a rapidly growing method in biomedical research allowing molecular mapping of proteins on histological sections. The images can be analyzed in terms of spectral pattern to define regions of interest. However, the identification and the differential quantitative analysis of proteins require off line or in situ proteomic methods using enzymatic digestion. The rapid identification of biomarkers holds great promise for diagnostic research but the major obstacle is the absence of rapid and direct method to detect and identify with a sufficient dynamic range a set of specific biomarkers. In the current work, we present a proof of concept for a method allowing identifying simultaneously a set of selected biomarkers on histological slices with minimal sample treatment using in-source decay (ISD) MSI and MALDI-Fourier transform ion cyclotron resonance (FTICR). In the proposed method, known biomarkers are spotted next to the tissue of interest, the whole MALDI plate being coated with 1,5-DAN matrix. The latter enhances MALDI radical-induced ISD, providing large tags of the amino acid sequences. Comparative analysis of ISD fragments between the reference spots and the specimen in imaging mode allows for unambiguous identification of the selected biomarker while preserving full spatial resolution. Moreover, the high resolution/high mass accuracy provided by FTICR mass spectrometry allows the identification of proteins. Well-resolved peaks and precise measurements of masses and mass differences allow the construction of reliable sequence tags for proteins identification. The method will allow the use MALDI-FTICR MSI as method for rapid targeted biomarker detection in complement to classical histology. [less ▲] Detailed reference viewed: 38 (5 ULg) Potential effects of blood contaminants on immune responses in harbour seals (Phoca vitulina)Dupont, Aurélie ; ; et alPoster (2011, August 23) Detailed reference viewed: 28 (3 ULg)POTENTIAL EFFECTS OF BLOOD CONTAMINANTS ON IMMUNE RESPONSES IN HARBOUR SEALS (PHOCA VITULINA)Dupont, Aurélie ; ; et alin Organohalogen Compounds (2011), 73 Detailed reference viewed: 27 (7 ULg)2D DIGE, label free quantification, principal component and mass spectrometry analysis for biomarkers discovery in MCF-7/BOS cells exposed to 17β-estradiol and endocrine disruptors.Collodoro, Mike ; ; Eppe, Gauthier et alin Organohalogen Compounds (2011) Endocrine system disruption has become a subject of great interest over the last few decades, since it has become evident that natural and also synthetic substances can mimic or reduce the activity of ... [more ▼] Endocrine system disruption has become a subject of great interest over the last few decades, since it has become evident that natural and also synthetic substances can mimic or reduce the activity of endogenous hormones. Compounds with estrogenic activity are an important family of potential endocrine disruptors that have to be monitored either in the food chain or in the environment. Estrogens are known to induce or promote hormonal dependent cancers, to reduce sperm counts and fertility in men and generate the feminization of exposed wildlife populations. The rapid screening of unwanted chemicals in the food chain is beset by difficulties. The number of toxic compounds is very large and no universal method can cope with their diversity. In this work, emergent differential proteomic techniques are used to discover a set of biomarkers for the development of a multiple estrogen contaminants screening test. [less ▲] Detailed reference viewed: 24 (2 ULg)Methylmercury in vitro exposure of harbor seal (Phoca vitulina) lymphocytes: a multidisciplinary approachDupont, Aurélie ; ; et alPoster (2009, June 09) Detailed reference viewed: 16 (2 ULg)Techniques de culture de cellules et de tissusDe Pauw-Gillet, Marie-Claire Learning material (2002) Detailed reference viewed: 12 (1 ULg)Confrontation in tridimensional culture of mouse B16 melanoma cells and 3T3 fibroblasts; De Pauw-Gillet, Marie-Claire ; Foidart, Jean-Michel et alin Cytotechnology (1988), (Suppl), 32 Detailed reference viewed: 5 (3 ULg)Co-cultures of melanoma cells and fibroblasts in three dimensionsDe Pauw-Gillet, Marie-Claire ; ; et alConference (1987) |
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