References of "DUBOIS, Nathalie"
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See detailEfficacy of heroin-assisted treatment in Belgium: a randomised controlled trial
Demaret, Isabelle ULg; Quertemont, Etienne ULg; Litran, Géraldine et al

in European Addiction Research (in press)

Background/Aims: Heroin-assisted treatment (HAT) can improve the condition of heroin addicts still using street heroin after a methadone treatment. In Belgium, a new trial compared the efficacy of a HAT ... [more ▼]

Background/Aims: Heroin-assisted treatment (HAT) can improve the condition of heroin addicts still using street heroin after a methadone treatment. In Belgium, a new trial compared the efficacy of a HAT to existing methadone maintenance treatment. Methods: In this randomised controlled trial, HAT was limited to 12 months. Participants were assessed every 3 months. They were responders if they showed improvement on the level of street heroin use, health or criminal involvement. Results: 74 participants were randomised in the trial. The experimental group (n=36) counted 30% of responders more than the control group (n=38) at each assessment point (p<0.05), except at 12 months where the difference (11%) was no longer significant (p=0.35). Still, after 12 months, participants in the experimental group reported significantly greater improvements (p<0.05) than the control group on the level of street heroin use and on the level of physical and mental health. Both groups reported significantly less criminal facts after 12 months (p<0.001), but with no significant difference between the groups. Conclusions: This trial confirms the short-term efficacy of HAT for severe heroin addicts, who already failed methadone treatment. [less ▲]

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See detailValidation analytique d'une méthode chromatographique destinée à rechercher et à identifier les opiacés naturels ou (semi) synthétiques
DUBOIS, Nathalie ULg; Counerotte, Stéphane ULg; Goffin, Eric ULg et al

in Annales de Biologie Clinique (2014), 72(2), 197-206

L’identification de la substance absorbée par un consommateur d’opiacés peut être problématique dans la mesure où il n’existe pas de biomarqueur spécifique pour toutes les molécules. Nous avons développé ... [more ▼]

L’identification de la substance absorbée par un consommateur d’opiacés peut être problématique dans la mesure où il n’existe pas de biomarqueur spécifique pour toutes les molécules. Nous avons développé une technique de chromatographie liquide ultra-haute pression couplée à un spectromètre de masse en tandem qui permet l’identification et le dosage de 25 opiacés dans le plasma. La préparation de l’échantillon consiste en une extraction en phase solide sur colonnes Oasis ® MCX (Waters). La méthode a été validée selon les critères préconisés par la FDA, complètement pour 21 substances et avec quelques réserves pour les 4 produits restants. Cette méthode a été appliquée à 80 patients traités au CHU de Liège pour lesquels la recherche d’opiacés était positive. L’identification du produit consommé a été effective dans 86 % des cas. [less ▲]

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See detailSimultaneous determination of seven azole antifungal drugs in serum by ultra-high pressure liquid chromatography and diode array detection
MISTRETTA, Virginie ULg; DUBOIS, Nathalie ULg; DENOOZ, Raphael ULg et al

in Acta Clinica Belgica (2014), 69(1), 53-61

Azole antifungals are a group of fungistatic agents that can be administered orally or parenterally. The determination of the concentrations of these antifungals (miconazole, fluconazole, ketoconazole ... [more ▼]

Azole antifungals are a group of fungistatic agents that can be administered orally or parenterally. The determination of the concentrations of these antifungals (miconazole, fluconazole, ketoconazole, posaconazole, voriconazole, itraconazole, and its major active metabolite, hydroxy-itraconazole) in serum can be useful to adapt the doses to pharmacological ranges because of large variability in the absorption and metabolism of the drugs, multiple drug interactions, but also potential resistance or toxicity. A method was developed and validated for the simultaneous determination of these drugs in serum utilizing ultra-high pressure liquid chromatography and diode array detection (UHPLC-DAD). After a simple and rapid liquid– liquid extraction, the pre-treated sample was analysed on an UHPLC-DAD system (Waters CorporationH). The chromatographic separation was carried out on an Acquity BEH C18 column (Waters Corporation) with a gradient mode of mobile phase composed of acetonitrile and aqueous ammonium bicarbonate 10.0 M pH10. The flow rate was 0.4 ml/min and the injection volume was 5 ml. The identification wavelength varied according to the drug from 210 to 260 nm. The method was validated by the total error method approach by using an analytical validation software (eNnoval V3.0 ArlendaH). The seven azole antifungals were identified by retention time and specific UV spectra, over a 13-minute run time. All calibration curves showed good linearity (r2.0.99) in ranges considered clinically adequate. The assay was linear from 0.05 to 10 mg/l for voriconazole, posaconazole, itraconazole, hydroxy-itraconazole, and ketoconazole, from 0.3 to 10 mg/l for fluconazole, and from 0.1 to 10 mg/l for miconazole. The bias and imprecision values for intraand inter-assays were lower than 10% and than 15%, respectively. In conclusion, a simple, sensitive, and selective UHPLC-DAD method was developed and validated to determine seven azole antifungal drugs in human serum. This method is applicable to patient samples, and can be applied successfully to clinical applications and therapeutic drug monitoring. [less ▲]

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See detailSimultaneous determination of some phthalate metabolites, parabens and benzophenone-3 in urine by ultra high pressure liquid chromatography tandem mass spectrometry
Dewalque, Lucas ULg; PIRARD, Catherine ULg; DUBOIS, Nathalie ULg et al

in Journal of Chromatography. B : Analytical Technologies in the Biomedical & Life Sciences (2014), 949-950

Phthalates, parabens and 2-hydroxy-4-methoxybenzophenone or benzophone-3 are thought to act as endocrine disrupting chemicals, being able to disrupt the endocrine balance and therefore able to lead to ... [more ▼]

Phthalates, parabens and 2-hydroxy-4-methoxybenzophenone or benzophone-3 are thought to act as endocrine disrupting chemicals, being able to disrupt the endocrine balance and therefore able to lead to some hormonal diseases. Numerous large-scale biomonitoring studies have detected the biomarkers of these compounds in more than 75% of the general population. To assess the exposure to these chemicals, we developed an analytical method based on a Solid Phase Extraction (SPE) prior to ultra high pressure liquid chromatographytandem mass spectrometry (UPLC-MS/MS) for the simultaneous measurement of seven phthalate metabolites (monobenzyl phthalate, mono-n-butyl phthalate, mono-iso-butyl phthalate, mono-2-ethylhexyl phthalate, mono-2-ethyl-5-hydroxyhexyl phthalate, mono-2- ethyl-5-oxohexyl phthalate, monoethyl phthalate), four parabens (methyl paraben, ethyl paraben, n-propyl paraben, n-butyl parabens) and benzophenone-3 in human urine. The distinction between unconjugated, glucuro- and sulfoconjugated forms was achieved using different enzymatic hydrolyses. The whole procedure was validated according to the total error approach, and was demonstrated to be linear (regression coefficient ranging from 0.987 to 0.998) and accurate (inter and intra assay precision <17.71%, relative bias <5.87%) in the dosing range of concentrations. The limits of quantification (LOQs) obtained ranged between 0.30 and 1.23 ng/ml depending on the analyte. The reliability of the method was proven in passing successfully the German External Quality Assessment Scheme (G-EQUAS). Moreover, the urine from 25 volunteers were analyzed for the determination of glucuro-, sulfo- and free species separately. Phthalate metabolites, parabens and benzophenone-3 were positively detected in almost all urine samples, with detection rates ranging from 40 to 100%. Levels measured ranged from <LOQ to 2207 ng/ml varying widely depending on the compound and the individual. In our small participating population, most of the phthalate metabolites were excreted predominately as glucuroconjugated forms while parabens and benzophenone-3 were detected as glucuro- and sulfoconjugated species in variable proportions according to the target compound. [less ▲]

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See detailProjet TADAM: RAPPORT FINAL 2007-2013
Demaret, Isabelle ULg; Litran, Géraldine; Magoga, Cécile et al

Report (2013)

Background: Heroin-assisted treatment (HAT) can improve the condition of heroin addicts resistant to other treatment. A new trial compared HAT and methadone treatment with HAT limited to 12 months in ... [more ▼]

Background: Heroin-assisted treatment (HAT) can improve the condition of heroin addicts resistant to other treatment. A new trial compared HAT and methadone treatment with HAT limited to 12 months in order to assess its feasibility and efficacy in Belgium. Methods: TADAM (Treatment Assisted by Diacetylmorphine) was an open label randomised controlled trial. The experimental treatment was based upon the Swiss model of HAT developed in 1994. The primary efficacy criterion was determined by an improvement in street heroin use or in (physical or mental) health or a decrease in criminal involvement. Participants were assessed every 3 months. Self-reported data were complemented with toxicological analyses and criminal proceedings. Findings: 74 participants were randomised in the trial: 36 in the experimental group and 38 in the control group. The experimental group counted 30% of responders more than the control group at 3 months (p<0.05), 6 months (p<0.05), and 9 months (p<0.01). At 12 months, the number of responders was still higher in the experimental group (11%) but the difference was no longer significant (p=0.35). Street heroin use increased in the experimental group at the 12-month assessment just before the end of HAT. Conclusion: HAT is feasible and effective. However, HAT should not have a predetermined duration for heroin users for which heroin addiction became a chronic relapsing disease. Other data: In addition to the outcomes of the randomised controlled trial, the report contains other exploratory data and analysis: satisfaction of in treatment, criminological data, opinion of heroin users not included in the trial, opinion of caregivers and field workers (in the HAT centre, in the partner centres, and in other centres in the addiction field), impact of the HAT centre on its neighbourhood and a socio-economic evaluation. Funding: The TADAM trial was funded at 80% by the Federal Minister of Social Affairs and Public Health. It was also funded the City and the University of Liège. [less ▲]

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See detailNiveaux du plomb sanguin, du plomb urinaire, de l’acide δ-aminolévulinique et des porphyrines urinaires chez les personnes vivant à Kinshasa, R.D. Congo : une étude pilote de biosurveillance
Mputu Malolo, Corneille-Liévin; Ndelo di Phanzu, Josaphat; Marini Djang'Eing'A, Roland ULg et al

Conference (2013, October 18)

Objectives: Existing naturally in the earth’s crust, Lead is a widely used heavy metal. It is an environment toxicant that may deleteriously affect nervous, hematopoietic, skeletal, renal, endocrine and ... [more ▼]

Objectives: Existing naturally in the earth’s crust, Lead is a widely used heavy metal. It is an environment toxicant that may deleteriously affect nervous, hematopoietic, skeletal, renal, endocrine and reproductive systems. Lead is classified in its inorganic form as possible human carcinogen (group 2A) by IARC. Exposure to lead in the environment continues to be a serious public health problem for all ages. Children are particularly susceptible to lead poisoning. They absorb more lead from their environment and their developing central nervous systems are vulnerable to the toxicant. During the last twenty years, important measures of public health were undertaken in several countries to decrease lead exposure. In the best of our knowledge, this is not the case in D.R. Congo. A study indicated a relatively important lead impregnation of the Kinshasa population (mean 120 μg/L). However, there have been no reported studies in the evaluation of the relationship between urinary lead, urinary δ-aminolevulinic acid (δ-AlaU) and urinary porphyrins and lead blood level in Congolese people. This is the aim of this study targeting at first people living in Kinshasa. Methods: Blood lead and urinary lead levels were measured using inductively coupled plasma mass spectrometry. The Bio-Rad ALA/PBG by Column Test and spectrophotometer method were used to quantify the concentration of δ-Ala in urine. The separation of porphyrins was carried out by HPLC coupled with fluorescence detector. Results: 37% of studied population presented blood lead levels above the 100 μg/L threshold (geometric mean: 133.29 μg/L) with a higher concentration in women than in men (140.30 μg/L vs 130.78 μg/L). 50% of children (0-17 years) presented blood lead levels above the 100 μg/L threshold and 43% of the same population presented blood lead levels above 50 μg/L as accepted nowadays in US. In the adult population, some targeted occupations were found to be associated with high blood lead. A small correlation was observed between urinary lead and blood lead, but no correlation was noticed between δ-AlaU and Porphyrins with lead blood levels. Conclusion: This study confirmed a relatively important Pb impregnation of the Kinshasa population and the existence of a major public health issue requiring corrective actions and the implementation of an appropriate regulation. Also, urinary lead, urinary δ-Ala and urinary porphyrins seems to not to be sensitive markers for monitoring exposure to lead. [less ▲]

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See detailDosage de la cocaïne, des opiacés et des amphétamines dans le sérum par LC/MSMS
DUBOIS, Nathalie ULg

Conference (2013, September)

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See detailSevrage rapide aux opiaces sous anesthesie (RODA).
DUBOIS, Nathalie ULg; Hallet, C.; Luppens, D. et al

in Revue medicale de Liege (2013), 68(5-6), 298-302

Rapid Opiate Detoxification under Anesthesia (RODA) involves the use of opiate antagonists combined with anesthesia and pharmacotherapy to reduce withdrawal symptoms. The aim of our study was to measure ... [more ▼]

Rapid Opiate Detoxification under Anesthesia (RODA) involves the use of opiate antagonists combined with anesthesia and pharmacotherapy to reduce withdrawal symptoms. The aim of our study was to measure the plasma concentrations of heroin metabolites and methadone during anesthesia and patient stay at the hospital in order to assess the amount of active substances at each protocol step. Plasma concentrations of antagonists were also quantified and compared to the recommended target values. Blood samples were drawn in 10 patients undergoing RODA at different times of the procedure (during anesthesia, in post-anesthesia care unit and in psychiatry unit). The plasma concentrations of heroin metabolites, methadone and antagonists were measured using a previously described method. Heroin active metabolites were no longer detected in the patient blood when helshe left the hospital; by contrast, methadone was still present at significant concentrations 3 days after the beginning of the detoxification procedure. Naltrexone analysis allowed us to adjust doses to insure opiate receptor blockade during acute withdrawal, which is a critical period. [less ▲]

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See detailEvaluation de l'exposition professionnelle aux cytostatiques des travailleurs de deux services du CHU de Liège
Rusu, Dorina; DUBOIS, Nathalie ULg; Surleraux, Cécile et al

in Acta Clinica Belgica (2013), 68(6), 472

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See detailEvaluation de l'exposition professionnelle aux cytostatiques des travailleurs de deux services du CHU de Liège
Rusu, Dorina; DUBOIS, Nathalie ULg; Surleraux, Cécile et al

in Acta Clinica Belgica (2013), 68(6), 472

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See detailQuantitative determination of non persistent endocrine disruptors in human urine
Dewalque, Lucas ULg; PIRARD, Catherine ULg; DUBOIS, Nathalie ULg et al

in Acta Clinica Belgica (2013), 68(6),

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See detailBlood lead, urinary lead, urinary δ-aminolevulinic acid and urinary porphyrins levels among people living in kinshasa, D.R. Congo : a pilot biomonitoring study
Mputu Malolo, Corneille-Liévin; Ndelo di Phanzu, Josaphat; Marini Djang'Eing'A, Roland ULg et al

in Acta Clinica Belgica (2013), 68(6), 475

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