Transposition en réseau des trajets cliniques d’un Service de Radiothérapie dans le modèle de Reason pour la prévention et la gestion des évènements indésirables

Conference (2012)

Implementation of a continuing education program based on the process improvement methodology department.

Conference (2012)

A method for evaluating weaknesses and critical steps in the Radiation Treatment Process through Precursor Events reporting

Poster (2011, September)

objectives: to establish a method based on the reporting of precursor events to detect and to assess weak steps in the Radiation Treatment process. These steps are categorized according to Work domains ... [more ▼]

objectives: to establish a method based on the reporting of precursor events to detect and to assess weak steps in the Radiation Treatment process. These steps are categorized according to Work domains, functional Basic Responsibilities and levels of Severity of precursor events [less ▲]

Démarches dans le cadre de l’amélioration globale de la Qualité et de la Sécurité dans le service de Radiothérapie

Poster (2011)

Implementation of an Experience feed back committee (EFBC) and impact on prevention and risk management in radiotherapy.

Poster (2011)

Systematic chromosomal aberrations found in murine bone marrow-derived mesenchymal stem cells.

in Stem Cells & Development (2010), 19(8), 1167-1173

Mesenchymal stem cells (MSCs) are studied as a cellular source for the treatment of various diseases. In this work, we isolated and cultivated murine bone marrow-derived MSCs. After a first observation of ... [more ▼]

Mesenchymal stem cells (MSCs) are studied as a cellular source for the treatment of various diseases. In this work, we isolated and cultivated murine bone marrow-derived MSCs. After a first observation of a solid tumour in a mouse injected with these cells, we systematically explored their chromosomal stability. We observed in all the cytogenetically analysed cases gross chromosomal alterations every time the MSCs went through the senescence crisis while the lymphocytes from the same animals showed a normal chromosome count. This observation was confirmed in different mouse strains, with different culture protocols, and even in short-term cultures after an hematopoietic cell negative immunodepletion performed in order to accelerate the isolation procedure. Therefore, we conclude that murine MSCs display high chromosomal instability, can generate tumours, and that care must be taken before using them for the evaluation of MSC therapeutic potential. [less ▲]

VEGF111, a new VEGF-A isoform induced by genotoxic agents; resistance to proteolytic

Conference (2010)

Thérapie cellulaire de réparation tissulaire cardiaque par cellules souches hématopoïétiques et mésenchymateuses.

Doctoral thesis (2010)

Les scientifiques définissent les cellules souches (CS) en se basant sur 3 critères (Fig.1): (1) les CS sont douées de capacités d’auto-renouvellement qui leur permettent de maintenir leur « pool » stable ... [more ▼]

Les scientifiques définissent les cellules souches (CS) en se basant sur 3 critères (Fig.1): (1) les CS sont douées de capacités d’auto-renouvellement qui leur permettent de maintenir leur « pool » stable ; (2) une CS peut se différencier en plusieurs types de cellules fonctionnelles matures. Par exemple, les cellules souches hématopoïétiques (CSH) sont à l’origine des cellules sanguines ; les cellules souches neurales (CSN) donnent naissance aux neurones, astrocytes et oligodendrocytes ; les cellules souches mésenchymateuses (CSM) peuvent se différencier en fibroblastes, ostéoblastes, chondroblastes et adipocytes ; (3) les cellules souches peuvent repeupler un tissu lésé pour en restaurer la fonction, comme notamment, après une irradiation. [less ▲]

PRIX WALLON DE LA QUALITE 2009 : « La Qualité : ombre et lumière »

Article for general public (2009)

Cellular therapy for cardiac tissue repair by haematopoietic and mesenchymal stem cells

Master of advanced studies dissertation (2004)

A conceivable strategy in cell and gene therapy is the use of adult stem cells isolated from bone marrow. We defined the optimal conditions for the culture of human and murine mesenchymal stem cells (hMSC ... [more ▼]

A conceivable strategy in cell and gene therapy is the use of adult stem cells isolated from bone marrow. We defined the optimal conditions for the culture of human and murine mesenchymal stem cells (hMSC and mMSC). We observed that the MSC of both species had a special morphology thanks to which it was possible to isolate them in flow cytometry on the basis of the size and granulosity parameter only. We analyzed the MSC phenotype by flow cytometry and observed that the hMSC were CD31-, CD34-, CD45, CD80- and HLA-DR- while they expressed CD73, CD90, CD105 and HLA-1 antigens. mMSC phenotype was CD34-, CD45-, CD11b-, CD106+ and Sca-1+. We also wanted to determine the frequency in progenitors among the mMSC amplified in vitro. To do so, we first assessed the enrichment in CFU-F (Colony-Forming Units – Fibroblast) progenitors. This method consisted in a secondary culture in liquid medium optimized for the development of colonies of mesenchymal origin. We were able to observe an increase of the CFU-F during the MSC passages. Then, we developed a method in order to assess the progenitors frequency by culturing MSC at limiting dilutions (CFUF-IC, Colony-Forming Units Fibroblast-Initiating cells). Once again, we were able to notice that the frequency in progenitors increased during the successive passages. The ratio between the number of CFU-F and the frequency in progenitors amounted to ± 10. We also performed differentiation assays. We were able to differentiate the mMSC in fat cells, chondrocytes and osteoblasts. Finally, we developed a model of left coronary artery ligature in mice as well as immunohistochemical markers showing the antigens CD31, -actinin and connexin 43. With the intent to inject various types of grafts in this animal model, we also studied the cell cycle of the stem cells by Hoechst staining. Fluorescence analysis of mMSC isolated from EGFP transgenic mice revealed that their fluorescence increased from ± 30% in marrow to more than 90% for the mMSC isolated and amplified via an in vitro culture. [less ▲]