Antigenic Variants Of Bovine Leukemia-Virus (Blv) Are Defined By Amino-Acid Substitutions In The Nh2 Part Of The Envelope Glycoprotein-Gp51Portetelle, Daniel ; ; et alin Virology (1989), 169(1), Bovine leukemia virus (BLV) p24 gene was expressed in Saccharomyces cerevisiae under the control of the PHO5 (encoding repressible acid phosphatase, rAPase) promoter. Yeast cells were transformed by a ... [more ▼] Bovine leukemia virus (BLV) p24 gene was expressed in Saccharomyces cerevisiae under the control of the PHO5 (encoding repressible acid phosphatase, rAPase) promoter. Yeast cells were transformed by a yeast-E. coli shuttle vector carrying the PHO5 promoter, the p24 gene and the CYC1 transcription terminator. After low inorganic phosphate (Pi) induction of the PHO5 promoter, p24 accumulated in the producing cells up to a concentration representing 10% of total soluble proteins. The expression level of p24 gene was not increased by insertion of the positive regulatory gene PHO4 on the p24 expression vector. The p24 produced in this system and incubated in crude yeast extract showed a remarkably high resistance to proteolytic degradation, a feature that presumably correlates with the compact globular conformation of the protein combined to the stabilizing effect of the N-terminal residue. [less ▲] Detailed reference viewed: 8 (1 ULg) Bovine leukemia virus as an inducer of bovine leukemia; ; et al in Animal models of retrovirus infection and their relationship to aids (1986) Detailed reference viewed: 11 (6 ULg) Bovine leukemia virus, a distinguished member of the human T-Lymphotropic viruses family; ; et al in Retroviruses and human pathology (Humana Press Clifton, New Jersey) (1985) Detailed reference viewed: 5 (4 ULg) Bovine leukemia virus, a versatile agent with various pathogenic effects in various animal species; ; et al in Cancer Research. Supplement (1985) Detailed reference viewed: 2 (1 ULg) Bovine leukemia virus, a distinguished member of the human T-lymphotropic virus family; ; et al in Japan Sci. Soc.Press (Tokyo) / VNU Science Press (Utrecht) (1985) Detailed reference viewed: 11 (9 ULg) Oncogène et traitement du cancer; ; et al in Revue Médicale de Bruxelles (1985) Detailed reference viewed: 4 (1 ULg) Bovine leukemia virus : a versatile agent with various pathogenic effects in various animal species; ; et al in Revue de l'Institut Pasteur de Lyon (1985) Detailed reference viewed: 8 (2 ULg) Enzootic bovine leukemia : its relevance as a model system for humane T-cell leukemia viruses.; ; et al in Gallo, R. (Ed.) Human T-cell leukemia-lymphoma virus (1984) Detailed reference viewed: 8 (1 ULg) Integration sites of BLV provirus in the tumor cell genome : implications for the mechanism of leukemogenesis.; Kettmann, Richard ; et alin Straub, O. C. (Ed.) Proceedings of the Fifth international symposium on bovine leukosisgs (1984) Detailed reference viewed: 2 (0 ULg) Chromosome Integration Domain for Bovine Leukemia Provirus in TumorsKettmann, Richard ; ; et alin Journal of Virology (1983), 47(1), 146-150 Detailed reference viewed: 2 (0 ULg) Biochemical and immunological studies on the Bovine Leukemia Virus (BLV).Portetelle, Daniel ; ; Kettmann, Richard et alin Yohn, D.; Blakeslee, J. (Eds.) Advances in Comparative Leukemia Research 1981 (1982) Detailed reference viewed: 1 (0 ULg) |
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