References of "Connolly, Lisa"
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See detailTreatment of estrogens and androgens in dairy wastewater by a constructed wetland system.
Cai, Kai; Elliott, Christopher T; Phillips, Debra H et al

in Water Research (2012), 46(7), 2333-43

Constructed wetland systems (CWS) have been used as a low cost bio-filtration system to treat farm wastewater. While studies have shown that CWS are efficient in removing organic compounds and pathogens ... [more ▼]

Constructed wetland systems (CWS) have been used as a low cost bio-filtration system to treat farm wastewater. While studies have shown that CWS are efficient in removing organic compounds and pathogens, there is limited data on the presence of hormones in this type of treatment system. The objective of this study was to evaluate the ability of the CWS to reduce estrogenic and androgenic hormone concentration in dairy wastewater. This was achieved through a year long study on dairy wastewater samples obtained from a surface flow CWS. Analysis of hormonal levels was performed using a solid phase extraction (SPE) sample clean-up method, combined with reporter gene assays (RGAs) which incorporate relevant receptors capable of measuring total estrogenic or androgenic concentrations as low as 0.24 ng L(-1) and 6.9 ng L(-1) respectively. Monthly analysis showed a mean removal efficiency for estrogens of 95.2%, corresponding to an average residual concentration of 3.2 ng L(-1) 17beta-estradiol equivalent (EEQ), below the proposed lowest observable effect concentration (LOEC) of 10 ng L(-1). However, for one month a peak EEQ concentration of 115 ng L(-1) was only reduced to 18.8 ng L(-1). The mean androgenic activity peaked at 360 ng L(-1) and a removal efficiency of 92.1% left an average residual concentration of 32.3 ng L(-1) testosterone equivalent (TEQ). The results obtained demonstrate that this type of CWS is an efficient system for the treatment of hormones in dairy wastewater. However, additional design improvements may be required to further enhance removal efficiency of peak hormone concentrations. [less ▲]

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See detailRemoval of Androgens and Estrogens from Water by Reactive Materials
Kai, Cai; Phillips, D. H.; Elliott, Christian et al

in Journal of Water Resource and Protection (2010), 2(11), 990-993

Nowadays, endocrine disruptor compounds in the water system have become a concern due to the risk of contamination to wild life and humans even at the nanogram level. Excess estrogens and androgens are a ... [more ▼]

Nowadays, endocrine disruptor compounds in the water system have become a concern due to the risk of contamination to wild life and humans even at the nanogram level. Excess estrogens and androgens are a major contributor group of endocrine compounds. Statistical surveys have shown that dairy farms contribute to over 90% of the total estrogens in the UK and US. An analytical system is being developed to assess the efficiency of reactive materials to remove target hormonal contaminants from dairy farm effluent. This can be achieved using reporter gene assays (RGAs) to detect low level steroid hormones. A preliminary study comparing the efficiency of granular activated carbon, zero-valent iron, and organoclay was carried out using bench-scale evaluations in negative control HPLC water. Their potential ability to remove testosterone and 17-β- estradiol spiked at reported environmental levels was measured by androgenic and estrogenic luminescent reporter assay respectively. [less ▲]

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See detailRemoval of Androgens and Estrogens from Water by Reactive Materials
Kai, Cai; Phillips, D. H.; Elliott, Chris et al

in 2010 4th International Conference on Bioinformatics and Biomedical Engineering (iCBBE 2010) (2010)

Nowadays, endocrine disruptor compounds in the water system have become a concern due to the risk of contamination to wild life and humans even at the nanogram level. Excess estrogens and androgens are a ... [more ▼]

Nowadays, endocrine disruptor compounds in the water system have become a concern due to the risk of contamination to wild life and humans even at the nanogram level. Excess estrogens and androgens are a major contributor group of endocrine compounds. Statistical surveys have shown that dairy farms contribute to over 90% of the total estrogens in the UK and US. An analytical system is being developed to assess the efficiency of reactive materials to remove target hormonal contaminants from dairy farm effluent. This can be achieved using reporter gene assays (RGAs) to detect low level steroid hormones. A preliminary study comparing the efficiency of granular activated carbon, zero-valent iron, and organoclay was carried out using bench-scale evaluations in negative control HPLC water. Their potential ability to remove testosterone and 17- beta -estradiol spiked at reported environmental levels was measured by androgenic and estrogenic luminescent reporter assay respectively. [less ▲]

Detailed reference viewed: 51 (7 ULg)
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See detailDetection of glucocorticoid bioactivity in bovine urine samples using a reporter gene assay
Connolly, Lisa; Cai, Kai; Van Der Heiden, Edwige ULg et al

in Analytica Chimica Acta (2009), 637

The illegal use of anabolic substances in the meat producing industry is an ongoing problem due to the continual production of new synthetic compounds and/or the practice of lowlevel cocktail ... [more ▼]

The illegal use of anabolic substances in the meat producing industry is an ongoing problem due to the continual production of new synthetic compounds and/or the practice of lowlevel cocktail administration to avoid detection by the surveillance schemes of EU member states National Plan surveillance systems. We present a highly sensitive reporter gene assay and sample extraction procedure based on a two step solid phase extraction and high performance liquid chromatography, developed for the detection of glucocorticoid abuse in bovine urine. The assay is capable of detecting compounds with glucocorticoid bioactivity and is extremely sensitive with an EC50 of 0.79 ngmL−1 for dexamethasone. New or unknown compounds with glucocorticoid bioactivity and low-level cocktail mixtures are detectable by this assay. Cross-reactivity data for a range of 11 -hydroxyglucocorticoids has been provided. This assay shows low interference from the 11-keto prohormones and other steroidal hormones. The assay may be suitable for application in other matrices such as hair. In conclusion this screening assay offers advantages over existing analytical techniques. [less ▲]

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See detailDetection of glucocorticoid bioactivity in bovine urine samples using a reporter gene assay
Connolly, Lisa; Kai, C.; Van Der Heiden, Edwige ULg et al

in Proceedings of the Euroresidue VI Conference (2008)

Detailed reference viewed: 7 (0 ULg)