References of "Collodoro, Mike"
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See detailEffects of eccentrically and concentrically biased training on mouse muscle phenotype
Hody, Stéphanie ULg; Lacrosse, Zoé ULg; Leprince, Pierre ULg et al

in Medicine & Science in Sports & Exercise (2013), 45(8), 1460-1468

Introduction: The molecular adaptations specifically induced by different muscle contraction types have only been partially elucidated. We previously demonstrated that eccentric contractions in human ... [more ▼]

Introduction: The molecular adaptations specifically induced by different muscle contraction types have only been partially elucidated. We previously demonstrated that eccentric contractions in human quadriceps elicited proteome modifications that suggest a muscle fiber typology adaptation. We address this question in a more systematic way by examining here the effects of different running modes on the mouse muscle proteome and the muscle fiber typology. Methods: Male adult mice (C57BL6) were randomly divided into downhill running (DHR, quadricipital eccentrically biased contractions), uphill running (UHR, quadricipital concentrically biased contractions) and untrained control (CONT) groups. Running groups performed five training sessions on an inclined treadmill for 75 to 135 min/day and the quadriceps muscles were dissected 96hours after the last session. Muscle protein extracts of DHR and UHR groups (n=4/group) were subjected to a 2D-DIGE analysis coupled with mass spectrometry. The assessment of fiber type, size and number was performed on the rectus femoris of the three groups (n=6/group) using myosin heavy chain (MHC) immunohistochemistry. Results: In the proteomic analysis, eight spots identified as the fast MHC isoforms exhibited a lower abundance in DHR compared to UHR (p<0.05, t-test). In contrast, ATP synthase subunit α and tubulin β were more expressed in DHR (p<0.05). A significant higher proportion of type I and IIa fibers was found for DHR compared to UHR or CONT groups (p<0.05, one-way ANOVA). Conclusions: Our data suggest that the eccentrically biased contractions in mice induced specific adaptations in protein expression and muscle fiber composition which may reflect a more oxidative muscle phenotype. The differences in stress placed on the muscle between both trainings may be responsible for some unique adaptations resulting from the eccentrically biased training. [less ▲]

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See detailEffects of eccentric versus concentric training on mouse muscle phenotype
Hody, Stéphanie ULg; Lacrosse, Zoé ULg; Leprince, Pierre ULg et al

in Abstract Book of 10th Meeting of Belgian Society for Neuroscience (2013, May)

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See detailSecretion and maturation of conotoxins in the venom ducts of Conus textile
Dobson, Rowan ULg; Collodoro, Mike; Gilles, Nicolas et al

in Toxicon (2012), 60(8), 1370-1379

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See detailAn ESR, Mass Spectrometry and Fluorescence microscopy approach to study the stearic acid derivatives anchoring in cells
Grammenos, Angeliki ULg; Fillet, Marianne ULg; Collodoro, Mike et al

in Applied Magnetic Resonance (2012)

Lateral phase separations in biological membranes are of great interest, making Electron Spin Resonance (ESR) spectroscopy combined with spin labelling a non destructive and sensitive technique for the ... [more ▼]

Lateral phase separations in biological membranes are of great interest, making Electron Spin Resonance (ESR) spectroscopy combined with spin labelling a non destructive and sensitive technique for the study of lipid rafts. This is currently accepted that spin probe localization is on the plasma membrane. However, no study confirms this hypothesis. Herein, we report, for the first time, an accurate multi spectral method for the quantification of lipid spin label presence in every sub-cellular fraction. Cells were incubated with 5-doxyl stearic acid derivative and then sub-fractionated. Results of our multimodal spectroscopy approach ubiquitously demonstrate that the presence of ESR spin label only sets in the plasma membranes. [less ▲]

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See detailStealth macromolecular platforms for the design of MRI blood pool contrast agents
Grogna, Mathurin ULg; Cloots, Rudi ULg; Luxen, André ULg et al

in Polymer Chemistry (2011), 2(10), 2316-2327

Stealth macromolecular platforms bearing alkyne groups and poly(ethylene oxide) brushes were synthesized by reversible addition fragmentation chain transfer (RAFT) polymerization. The anchoring of Gd3 ... [more ▼]

Stealth macromolecular platforms bearing alkyne groups and poly(ethylene oxide) brushes were synthesized by reversible addition fragmentation chain transfer (RAFT) polymerization. The anchoring of Gd3+-chelates bearing an azide group was then carried out by the Huisgen 1,3-dipolar cycloaddition (“click”) reaction in mild conditions, leading to macrocontrast agents for MRI applications. The gadolinium complex is hidden in the PEO shell that renders the macrocontrast agents free of any cytotoxicity and stealth to proteins of the immune system. Relaxometry measurements have evidenced an improved relaxivity of the macrocontrast agent compared to ungrafted gadolinium chelate. Moreover, this relaxivity is further enhanced when the spacer length between the Gd3+-chelate and the polymer backbone is shorter, as the result of its decreased tumbling rate. These novel products are therefore promising candidates for MRI applications. [less ▲]

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See detailSecretion and maturation of toxins in the venom duct of Conustextile
Dobson, Rowan ULg; Corbesier, Corine; Collodoro, Mike et al

Conference (2009, December 02)

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See detailMaturation of toxins in the venom duct of conustextile
Dobson, Rowan ULg; Collodoro, Mike; Gilles, Nicolas et al

Poster (2009, June)

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See detailStudy of the effect of exposition of MCF-7/bos cells to 17-ß-estradiol by 2D DIGE
Collodoro, Mike; Dobson, Rowan ULg; Bertrand, Virginie ULg et al

Poster (2006, October)

The aim of this work is to identify biomarkers following exposition to 17ß-estradiol in MCF7/bos (hormonal dependent epithelial mammary cancer) cells. The application is to optimise screening tests that ... [more ▼]

The aim of this work is to identify biomarkers following exposition to 17ß-estradiol in MCF7/bos (hormonal dependent epithelial mammary cancer) cells. The application is to optimise screening tests that will enable the detection (and eventual quantification but not identification) of numerous compounds having an estrogenic activity in a single rapid test. [less ▲]

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