References of "Cognié, Y"
     in
Bookmark and Share    
Full Text
Peer Reviewed
See detailFirst birth of an animal from an extinct subspecies (Capra pyrenaica pyrenaica) by cloning
Folch, J.; Cocero, M. J.; Chesne, P. et al

in Theriogenology (2009), 71

Two experiments have been performed to clone the bucardo, an extinct wild goat. The karyoplasts were thawed fibroblasts derived from skin biopsies, obtained and cryopreserved in 1999 from the last living ... [more ▼]

Two experiments have been performed to clone the bucardo, an extinct wild goat. The karyoplasts were thawed fibroblasts derived from skin biopsies, obtained and cryopreserved in 1999 from the last living specimen, a female, which died in 2000. Cytoplasts were mature oocytes collected from the oviducts of superovulated domestic goats. Oocytes were enucleated and coupled to bucardo's fibroblasts by electrofusion. Reconstructed embryos were cultured for 36h or 7d and transferred to either Spanish ibex or hybrid (Spanish ibex malex domestic goat) synchronized recipients. Embryos were placed, according to their developmental stage, into the oviduct or into the uterine horn ipsilateral to an ovulated ovary. Pregnancy was monitored through their plasmatic PAG levels. In Experiment 1, 285 embryos were reconstructed and 30 of them were transferred at the 3- to 6-cells stage to 5 recipients. The remaining embryos were further cultured to day 7, and 24 of them transferred at compact morula/blastocyst stage to 8 recipients. In Experiment 2, 154 reconstructed embryos were transferred to 44 recipients at the 3- to 6-cells stage. Pregnancies were attained in 0/8 and 7/49 of the uterine and oviduct-transferred recipients, respectively. One recipient maintained pregnancy to term, displaying very high PAG levels. One morphologically normal bucardo female was obtained by caesarean section. The newborn died some minutes after birth due to physical defects in lungs. Nuclear DNA confirmed that the clone was genetically identical to the bucardo's donor cells. To our knowledge, this is the first animal born from an extinct subspecies. [less ▲]

Detailed reference viewed: 112 (2 ULg)
Full Text
Peer Reviewed
See detailMeasurement of ovine pregnancy-associated glycoprotein (PAG) during early pregnancy in Lacaune sheep
El Amiri, B.; Karen, A.; Sulon, Joseph ULg et al

in Reproduction in Domestic Animals (2007), 42(3), 257-262

This study describes ovine pregnancy-associated glycoprotein (ovPAG) concentrations in 20 Lacaune sheep during early pregnancy. Measurements were performed by using semi-purified ovPAG as standard, tracer ... [more ▼]

This study describes ovine pregnancy-associated glycoprotein (ovPAG) concentrations in 20 Lacaune sheep during early pregnancy. Measurements were performed by using semi-purified ovPAG as standard, tracer and immunogens for antibody production in rabbits. Antisera R780 (against ovPAG(57+59kDa)) and R805 (against ovPAG5(58+61kDa)) were used respectively in RIA-780 and RIA-805. Blood samples were collected at days 0, 18, 20, 22 and 25 after artificial insemination. From day 18 after breeding onward, the mean ovPAG concentration was significantly higher (p < 0.001) in plasma samples from pregnant ewes (n = 17) than in non-pregnant ones (n = 3). The specific activity of the tracer was 11 760 Ci/mmol in RIA-780 and 14 900 Ci/mmol in RIA-805. The minimal detection limits for RIA-780 and RIA-805 were 0.2 ng/ml and 0.3 ng/ml, respectively. The intra-assay CV of samples with low (1.0 ng/ml), medium (2.5 ng/ml) and high (4.0 ng/ml) PAG concentrations were 3%, 6% and 9% for RIA-780 and 8%, 9% and 5% for RIA-805. The inter-assay CV in the same samples were 13%, 12% and 7% for RIA-780 and 13%, 11% and 5% for RIA-805. The recovery was higher than 95% in both assays. No cross-reaction was observed with members of aspartic proteinase family as well as with other tested proteins. In both RIA-780 and RIA-805, inhibition of the binding of the tracer by antisera was parallel between standard curve and serial dilutions of pregnant ewe samples. In conclusion, the two homologous RIA systems are suitable for early quantification of ovPAG concentrations in ewe plasma samples from day 18 after breeding. [less ▲]

Detailed reference viewed: 40 (3 ULg)
Full Text
Peer Reviewed
See detailThe viability of in vitro produced sheep embryos is negatively affected by the presence of estradiol during in vitro maturation
Poulin, N.; Cognié, Y.; Baril, G. et al

in Reproduction in Domestic Animals (2005), 40(4), 92

Detailed reference viewed: 9 (2 ULg)
Full Text
Peer Reviewed
See detailEffet de prétraitements agoniste et antagoniste de GnRH sur la production d’embryons chez la brebis et la chèvre
Baril, G.; Cognié, Y.; Belloc, J. P. et al

in Proceedings: 11e Rencontres autour des Recherches sur les Ruminants (3R) (2004)

Detailed reference viewed: 15 (2 ULg)
Full Text
See detailDiagnostic et suivi de gestation chez la chèvre et la brebis
Melo de Sousa, Noelita ULg; Gonzalez, F.; Karen, A. et al

Scientific conference (2004)

Detailed reference viewed: 23 (4 ULg)
Full Text
Peer Reviewed
See detailPregnancy-associated glycoprotein concentrations in plasma and milk samples for early pregnancy diagnosis in Lacaune dairy sheep.
El Amiri, B.; Cognié, Y.; Sulon, J. et al

in Reproduction in Domestic Animals (2003), 38(4), 322

Detailed reference viewed: 7 (0 ULg)