References of "Closset, Rodrigue"
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See detailLung interstitial macrophages alter dendritic cell functions to prevent airway allergy in mice
Bedoret, Denis ULg; Wallemacq, Hugues ULg; Marichal, Thomas ULg et al

in Journal of Clinical Investigation (2009), 119(12), 3723-38

The respiratory tract is continuously exposed to both innocuous airborne antigens and immunostimulatory molecules of microbial origin, such as LPS. At low concentrations, airborne LPS can induce a lung DC ... [more ▼]

The respiratory tract is continuously exposed to both innocuous airborne antigens and immunostimulatory molecules of microbial origin, such as LPS. At low concentrations, airborne LPS can induce a lung DC-driven Th2 cell response to harmless inhaled antigens, thereby promoting allergic asthma. However, only a small fraction of people exposed to environmental LPS develop allergic asthma. What prevents most people from mounting a lung DC-driven Th2 response upon exposure to LPS is not understood. Here we have shown that lung interstitial macrophages (IMs), a cell population with no previously described in vivo function, prevent induction of a Th2 response in mice challenged with LPS and an experimental harmless airborne antigen. IMs, but not alveolar macrophages, were found to produce high levels of IL-10 and to inhibit LPS-induced maturation and migration of DCs loaded with the experimental harmless airborne antigen in an IL-10-dependent manner. We further demonstrated that specific in vivo elimination of IMs led to overt asthmatic reactions to innocuous airborne antigens inhaled with low doses of LPS. This study has revealed a crucial role for IMs in maintaining immune homeostasis in the respiratory tract and provides an explanation for the paradox that although airborne LPS has the ability to promote the induction of Th2 responses by lung DCs, it does not provoke airway allergy under normal conditions. [less ▲]

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See detailExpression microarrays in equine sciences
Ramery, Eve ULg; Closset, Rodrigue; Art, Tatiana ULg et al

in Veterinary Immunology and Immunopathology (2009), 127(3-4), 197-202

Microarrays have become an important research tool for life science researchers. Expression microarrays are capable of profiling the gene expression pattern of tens of thousands of genes in a single ... [more ▼]

Microarrays have become an important research tool for life science researchers. Expression microarrays are capable of profiling the gene expression pattern of tens of thousands of genes in a single experiment. It appears to be the platform of choice for parallel gene expression profiling. Various equine-specific gene expression microarrays have been generated and used. However, homologous microarrays are not yet commercially available for the horse. An alternative is the use of heterologous microarrays, mainly microarrays specific for mice or humans. Although the use of microarrays in equine research is still in its infancy, gene expression microarrays have shown their potential in equine research. This review presents the previous, current and potential use of expression microarrays in equine research. [less ▲]

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See detailUsing a human microarray to highlight new genes of interest for a better understanding of molecular mechanisms that underpin the physiopathology of heaves
Ramery, Eve ULg; Closset, Rodrigue; Bureau, Fabrice ULg et al

in Proceedings: Plant and Animal Genome Conference, Equine Workshop, San Diego (2008)

Environmental causes of heaves are well described, but the molecular mechanisms of the disease remain unclear. Using reverse transcription polymerase chain reaction (RT-PCR), disparate results have been ... [more ▼]

Environmental causes of heaves are well described, but the molecular mechanisms of the disease remain unclear. Using reverse transcription polymerase chain reaction (RT-PCR), disparate results have been obtained concerning cytokines expression profile. cDNA microarray appears to be so far the platform of choice for massively parallel gene expression profiling and provides a good tool for exploratory research. However, equine-specific microarrays are not yet available on the market. Because they are commercially available, highly specific and well annotated, human and mouse large-scale microarrays are an exploratory alternative to equine-specific microarrays. In the present study, the purpose was to highlight new targets not previously related to the disease and able to improve our understanding of the molecular mechanisms of the disease. A human microarray was used to study gene expression in nucleated cells originating from peripheral blood and bronchoalveolar lavage fluid in heaves-affected horses. With a four-fold cut-off, a total of 46 candidates were identified with differentially regulated genes between heaves-affected horses and controls. Based on their documented function, five of these genes were selected for the real-time quantitative RT-PCR (RT-qPCR) validation procedure: CYBB, BTG1, MARCKS, PTX3 and PTPRC. The RT-qPCR results confirmed those obtained with the microarray, pointing out these genes as new directions for future experiments. However, the human microarray failed to detect the presence of IL-1beta and Il-8, otherwise confirmed by RT-qPCR, and the expression profile of the disease could not be obtained [less ▲]

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See detailRelevance of using a human microarray to study gene expression in heaves-affected horses.
Ramery, Eve ULg; Closset, Rodrigue; Bureau, Fabrice ULg et al

in Veterinary Journal (2008), 177(2), 216-221

Environmental causes of heaves are well described, but the molecular mechanisms of the disease remain unclear. Previous studies have highlighted the implications of variations in gene expression, most ... [more ▼]

Environmental causes of heaves are well described, but the molecular mechanisms of the disease remain unclear. Previous studies have highlighted the implications of variations in gene expression, most using reverse transcription polymerase chain reaction (RT-PCR). This well-known technique limits the number of genes that can be studied in a single assay. Microarray appears to be a valuable tool to by-pass this limitation, but so far there has been no equine-specific microarray available on the market. The present study was performed to determine whether a human microarray could be used to study gene expression in nucleated cells originating from peripheral blood and bronchoalveolar lavage fluid (BALF) in heaves-affected horses. With a four-fold cut-off, a total of 46 candidates were identified with differentially regulated genes between heaves-affected horses and controls. A real-time quantitative RT-PCR (RT-QPCR) conducted on a selection of genes, determined on the basis of previous publications, was used to validate the microarray results. The microarray failed to detect the presence of interleukin (IL)-1beta and IL-8 mRNA in the nucleated cells from BALF otherwise confirmed by real-time RT-QPCR. Although some candidate genes have been identified using this method, a complete expression profile of genes related to heaves could not be obtained with the use of the human microarray. [less ▲]

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See detailCaractériser un profil inflammatoire grâce à l'utilisation du microdamier
Ramery, Eve ULg; Closset, Rodrigue; Bureau, Fabrice ULg et al

Poster (2007)

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See detailIdentification de gènes d'intérêt pour le traitement du cheval poussif
Ramery, Eve ULg; Closset, Rodrigue; Salinas, Emmanuelle et al

in Proceedings: AVEF, Versailles, France (2006)

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