  Flow cytometric method for the detection of gpI antigens of varicella zoster virus and evaluation of anti-VZV agents; ; Sadzot-Delvaux, Catherine  et alin Journal of Virological Methods (1992), 38(2), 243-254 Varicella zoster virus (VZV) is responsible for a primary infection (varicella.) and, upon reactivation, zoster, which in immunocompromised patients, may both lead to life-threatening disseminated disease ... [more ▼] Varicella zoster virus (VZV) is responsible for a primary infection (varicella.) and, upon reactivation, zoster, which in immunocompromised patients, may both lead to life-threatening disseminated disease. There is a great need for antiviral compounds that are effective inhibitors of VZV replication and for rapid and accurate methods for evaluating viral sensitivity to candidate anti-VZV drugs. With the monoclonal antibody (mAb) (VL8), which is directed against the gpI of VZV, and using the fluorescence-activated cell sorter (FACS) we could readily demonstrate expression of the VZV gpI antigen at 3-4 days after VZV infection. (E)-5-(2-Bromovinyl)-2'-deoxyuridine (BVDU), (S)-9-(3-hydroxy-2-phosphonylmethoxypropyl)adenine (HPMPA) and (S)-1-(3-hydro-xy-2-phosphonylmethoxypropyl)cytosine (HPMC) were shown to be potent inhibitors of VZV replication by this assay. HPMPA and HPMPC were also active against thymidine kinase-deficient (TK-) VZV whereas BVDU was not. The flow cytometric method based on the use of mAb VL8 may be of considerable help for the early diagnosis of VZV infection and evaluation of viral sensitivity to antiviral drugs. [less ▲] Detailed reference viewed: 8 (0 ULg) Granulomatous reactions following herpes-zoster contain varicella-zoster glycoprotein GPINikkels, Arjen ; Sadzot-Delvaux, Catherine ; et alin Journal of Investigative Dermatology (1992), 98(4), 522 Detailed reference viewed: 7 (1 ULg)Preparation of reproducible alkaline phosphatase-antibody conjugates for enzyme immunoassay using a heterobifunctional linking agent; ; et al in Analytical Biochemistry (1988), 172(2), 392-396 Conjugates of alkaline phosphatase (AP) and mouse monoclonal immunoglobulins G (IgG) were prepared by means of the heterobifunctional linker, N-succinimidyl 3-(2-pyridyldithio)-propionate. The efficiency ... [more ▼] Conjugates of alkaline phosphatase (AP) and mouse monoclonal immunoglobulins G (IgG) were prepared by means of the heterobifunctional linker, N-succinimidyl 3-(2-pyridyldithio)-propionate. The efficiency of such conjugates can be improved by optimizing the degree of substitution of IgG and AP. We have determined conditions yielding better performing conjugates than those synthesized by methods described previously. Moreover, the results obtained with the technique presented here are quite reproducible with all four monoclonal antibodies tested. [less ▲] Detailed reference viewed: 57 (1 ULg)Comparison of conjugation procedures for the preparation of monoclonal antibody-enzyme conjugates; ; et al in Journal of Immunological Methods (1988), 111(2), 261-270 Four monoclonal antibodies belonging to different subclasses and with differing isoelectric points were coupled to horseradish peroxidase (HRP) and alkaline phosphatase (AP) using various conjugation ... [more ▼] Four monoclonal antibodies belonging to different subclasses and with differing isoelectric points were coupled to horseradish peroxidase (HRP) and alkaline phosphatase (AP) using various conjugation procedures. The conjugates were tested by enzyme immunoassay and their efficiency was characterized by the antibody and enzyme concentrations needed to obtain an arbitrary OD value. The suitability of antibody for conjugation through NH2 groups was tested by fluorodinitrobenzene (FDNB). HRP conjugates were produced by two variants of the sodium periodate procedure and two variants of the glutaraldehyde method, as well as by the heterobifunctional linker N-succinimidyl 3-(2-pyridyldithio)pro-pionate (SPDP). Two of the four antibodies were coupled by a third variant of the periodate method, through their carbohydrate moieties. The periodate-mediated conjugations, using sugar moieties on the enzyme, provided the most efficient HRP conjugates, regardless of the antibody subclass or isoelectric point. The glutaraldehyde procedures consistently gave the worst results. AP conjugates were prepared using the same methods. The most efficient and reproducible AP conjugates with all four monoclonal antibodies were obtained using the SPDP procedure. The efficiency of the other methods differed from one antibody to another. [less ▲] Detailed reference viewed: 26 (0 ULg)Set up of an easy selective method for the isolation of hybrid hybridomas. Evidence for the production of heterobifunctional monoclonal antibodies; ; et al in Archives Internationales de Physiologie, de Biochimie et de Biophysique (1988), 96 Detailed reference viewed: 7 (2 ULg)Production of heterobispecific monoclonal antibodies by mouse hybrid hybridomas (quadromas); Herens, Christian ; Nys, Monique et alin Archives Internationales de Physiologie, de Biochimie et de Biophysique (1988), 96 Detailed reference viewed: 31 (8 ULg) |
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