References of "Clinquart, Antoine"
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See detailIn vitro evaluation of the competing effect of Carnobacterium maltaromaticum isolated from vacuum packed meat against food pathogens
Maia Danielski, Gabriela ULiege; Didimo Imazaki, Pedro Henrique ULiege; Daube, Georges ULiege et al

Conference (2017, August 15)

Foodborne disease outbreaks are one of the leading causes of infections, hospitalizations and deaths provoked by pathogenic bacteria including Escherichia coli O157:H7, Listeria monocytogenes and ... [more ▼]

Foodborne disease outbreaks are one of the leading causes of infections, hospitalizations and deaths provoked by pathogenic bacteria including Escherichia coli O157:H7, Listeria monocytogenes and Salmonella spp. Carnobacterium maltaromaticum is a lactic acid bacteria, which could prevent the growth of pathogens in refrigerated food. The aim of this study was to determine the bioprotective potential of three strains of C. maltaromaticum isolated from chilled vacuum packed beef. They were tested in vitro against the pathogens described above. The results indicate that the selected strains have an antilisterial activity, which is optimized at low temperatures. Moreover, when the strains were combined with EDTA it was observed a slight, but significant, inhibition of the gram-negative bacteria used in this study. [less ▲]

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See detailInfluence of aging time and technique (dry- vs. wet-aging) on tenderness, color and lipid stability of Belgian Blue beef
Didimo Imazaki, Pedro Henrique ULiege; Teixeira Gonçalves, André ULiege; Krantz, Mike et al

Conference (2016, December 08)

The aim of this study was to compare the effect of aging conditions (dry-aging vs. wet-aging) and time (0, 21, 42 and 63 days) on tenderness, color and lipid stability of Belgian Blue beef. Three ... [more ▼]

The aim of this study was to compare the effect of aging conditions (dry-aging vs. wet-aging) and time (0, 21, 42 and 63 days) on tenderness, color and lipid stability of Belgian Blue beef. Three longissimus dorsi of Belgian Blue cows were dry- or wet-aged for up to 63 days at 2 °C. At different times, part of these samples was cut into steaks, vacuum packaged and stored during 4 days at 4 °C + 8 days at 8 °C. The following parameters were evaluated at different intervals: pH, tenderness (Warner–Bratzler peak shear force), color (CIE L*a*b*), myoglobin oxidation (K/S572:K/S525 ratio) and lipid oxidation (TBARS). The sensitivity of samples to pigment oxidation was influenced by the packaging during display. Aging time and packaging during display influenced lipid oxidation. Twenty-one days of aging allowed to achieve the maximum tenderness observed and would be compatible with a subsequent 12-day display under vacuum. A 12-day display period under shrinkable film is largely beyond the shelf life of meat under this condition. This study contributes new knowledge about Belgian Blue beef behavior when it is wet or dry aged. Further research will be conducted to study the microbiological quality of these meats. [less ▲]

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See detailChemical composition and antimicrobial activity of essential oils of Ocimum basilicum, Ocimum canum and Ocimum gratissimum in function of harvesting time
Mith, Hasika; Yayi-Ladékan, Eléonore; Dosso Sika Kpoviessi, Salomé et al

in Journal of Essential Oil-Bearing Plants [=JEOBP] (2016), 19(6), 1413-1425

The chemical composition of essential oils obtained by hydrodistillation from aerial parts of Ocimum basilicum, Ocimum canum and Ocimum gratissimum from Benin as affected by harvesting time, were analyzed ... [more ▼]

The chemical composition of essential oils obtained by hydrodistillation from aerial parts of Ocimum basilicum, Ocimum canum and Ocimum gratissimum from Benin as affected by harvesting time, were analyzed by GC-FID (Gas chromatography-Flame ionization detector) and GC-MS (Gas chromatography-Mass spectrometry). Based on the composition analysis, major components were as follows: estragol (43.0-44.7%) and linalool (24.6 29.8%) in O. basilicum oils; carvacrol (12.0-30.8%) and p-cymene (19.5-26.2%) in O. canum oils; thymol (28.3-37.7%) and γ-terpinene (12.5-19.3 %) in O. gratissimum oils. Disc diffusion and broth microdilution assays were used to evaluate the antibacterial activity of essential oils and their main components against two foodborne bacteria, Listeria monocytogenes and Salmonella enterica serotype Typhimurium. The tested oils and their components exhibited notable antimicrobial activities against L. monocytogenes and S. Typhimurium. The O. canum and O. gratissimum oils collected at 7h and 19h showed significant higher activities against L. monocytogenes and S. Typhimurium (MICs and MBCs 0.34 - 2.5 μL/mL) (p < 0.05), whereas O. basilicum showed lower activity (MICs and MBCs 2.0 - 8.0 μL/mL) at any daytime of harvest, the weakest being at 19h (MIC and MBC 12.0 - 32.0 μL/mL). The daytime of harvest can influence the composition of oils and their activities on bacteria. [less ▲]

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See detailWet-aging vs. Dry-aging : influence sur la tendreté et la stabilité oxydative des viandes Charolaises
Didimo Imazaki, Pedro Henrique ULiege; Teixeira Gonçalves, André ULiege; Krantz, Mike et al

Poster (2016, November)

The aim of this study was to compare the effect of aging conditions (dry-aging vs. wet-aging) and time (7, 28, 49 and 70 days) on tenderness, color and lipid stability of Charolais beef. Three longissimus ... [more ▼]

The aim of this study was to compare the effect of aging conditions (dry-aging vs. wet-aging) and time (7, 28, 49 and 70 days) on tenderness, color and lipid stability of Charolais beef. Three longissimus dorsi of Charolais cows were dry- or wet-aged for up to 70 days at 2 °C. At different times, part of these samples was cut into steaks, vacuum packaged and stored during 4 days at 4 °C + 8 days at 8 °C. The following parameters were evaluated at different intervals: pH, tenderness (Warner–Bratzler peak shear force), color (CIE L*a*b*), myoglobin oxidation (K/S572:K/S525 ratio) and lipid oxidation (TBARS). No difference in tenderness was observed between the two aging techniques. The sensitivity of samples to oxidation was influenced by the aging technique (wet-aging > dry-aging). For the studied samples, aging beyond 28 days could be considered excessive from an organoleptical point of view. [less ▲]

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See detailEffect of aging time, aging technique (dry- vs. wet-aging) and packaging on tenderness, pigment and lipid stability of Belgian blue beef
Didimo Imazaki, Pedro Henrique ULiege; Teixeira Gonçalves, André ULiege; Krantz, Mike et al

Poster (2016, October 21)

The aim of this study was to compare the effect of aging technique (dry-aging and wet-aging), aging time (0, 21, 42 and 63 days) and packaging during display (vacuum and shrinkable film wrapping) on pH ... [more ▼]

The aim of this study was to compare the effect of aging technique (dry-aging and wet-aging), aging time (0, 21, 42 and 63 days) and packaging during display (vacuum and shrinkable film wrapping) on pH, tenderness, pigment and lipid stability of beef. Three longissimus dorsi muscles from two Belgian Blue cows were dry- or wet-aged for up to 63 days at 2 °C. At different times, part of these samples was cut into steaks and repackaged in vacuum bags or shrinkable film, and stored during 4 days at 4 °C + 8 days at 8 °C (simulated retail display). The following parameters were evaluated at different intervals: pH, tenderness (Warner–Bratzler shear force), color (CIE L*a*b*), myoglobin oxidation (K/S 572/525 ratio) and lipid oxidation (TBARS). The aging technique and the packaging during simulated retail display had an effect on pH (dry-aging > wet-aging, P < 0.05; shrinkable film > vacuum, P < 0.05). An increase of tenderness was observed during the first 21 days of aging (P < 0.05). The sensitivity of samples to pigment oxidation was influenced by the packaging during display (shrinkable film > vacuum, P < 0.05). Aging time and packaging during display increased lipid oxidation (42 and 63 > 0 and 21 days, P < 0.05; plastic wrap > vacuum, P < 0.05). This study contributes new knowledge about Belgian Blue beef behavior whether it is wet- or dry-aged. Further research will be conducted to study the antioxidant capacity of these meats in order to better understand the oxidation process. [less ▲]

Detailed reference viewed: 34 (4 ULiège)
See detailLa viande, questions de vies et de morts ?
Clinquart, Antoine ULiege; Vandenheede, Marc ULiege; Von Hoffmann, Viktoria ULiege

Conference given outside the academic context (2016)

Detailed reference viewed: 48 (1 ULiège)
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See detailSensory quality of beef patties inoculated with strains of Carnobacterium maltaromaticum with potential as biopreservatives
Didimo Imazaki, Pedro Henrique ULiege; Jacques-Houssa, Charlotte ULiege; Kergourlay, Gilles ULiege et al

Conference (2015, December 02)

Biopreservation is the use of naturally occurring microorganisms and/or their inherent antimicrobial compounds to extend shelf life and to enhance the safety of foods. The aim of the present study was to ... [more ▼]

Biopreservation is the use of naturally occurring microorganisms and/or their inherent antimicrobial compounds to extend shelf life and to enhance the safety of foods. The aim of the present study was to perform a sensory evaluation of beef patties inoculated with potentially biopreservative strains of Carnobacterium maltaromaticum. Three different strains of C. maltaromaticum (lab. ref.: CM_824, CM_827 and CM_829) isolated from vacuum packaged beef with long shelf life were selected for this study. An untrained panel was requested to make a sensory evaluation of raw and cooked beef patties 8 and 10 days after inoculation with the selected strains at 104 and 106 UFC/g and storage in high-O2 atmosphere. After 8 days of storage, non inoculated samples (blank) were perceived as having the best studied sensory descriptors. The samples inoculated with strain CM_827 had a sensory quality very close to the blank. After 10 days of storage, samples inoculated with the strain CM_827 at 104 UFC/g received the highest scores for appearance and color. This study permitted to evaluate the effect of three C. maltaromaticum strains on the sensory quality of beef patties. Strain CM_827 did practically not change the sensory attributes of beef patties. Samples inoculated with strain CM_824 and CM_829 received the worst scores for several of the tested descriptors. Therefore, further research on the biopreservative capacity of C. maltaromaticum should be conducted with strain CM_827. [less ▲]

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See detailEvaluation of the sensory quality of beef patties inoculated with Carnobacterium maltaromaticum strains with biopreservative potential
Didimo Imazaki, Pedro Henrique ULiege; Jacques-Houssa, Charlotte ULiege; Kergourlay, Gilles ULiege et al

Conference (2015, October 16)

Biopreservation is the use of naturally occurring microorganisms and/or their inherent antimicrobial compounds to extend shelf life and to enhance the safety of foods. The aim of the present study was to ... [more ▼]

Biopreservation is the use of naturally occurring microorganisms and/or their inherent antimicrobial compounds to extend shelf life and to enhance the safety of foods. The aim of the present study was to perform a sensory evaluation of beef patties inoculated with potentially biopreservative strains of Carnobacterium maltaromaticum. Three different strains of C. maltaromaticum (lab. ref.: CM_824, CM_827 and CM_829) isolated from vacuum packaged beef with long shelf life were selected for this study. An untrained panel was requested to make a sensory evaluation of raw and cooked beef patties 8 and 10 days after inoculation with the selected strains at 104 and 106 UFC/g and storage in high-O2 atmosphere. After 8 days of storage, non inoculated samples (blank) were perceived as having the best studied sensory descriptors. The samples inoculated with strain CM_827 had a sensory quality very close to the blank. After 10 days of storage, samples inoculated with the strain CM_827 at 104 UFC/g received the highest scores for appearance and color. This study permitted to evaluate the effect of three C. maltaromaticum strains on the sensory quality of beef patties. Strain CM_827 did practically not change the sensory attributes of beef patties. Samples inoculated with strain CM_824 and CM_829 received the worst scores for several of the tested descriptors. Therefore, further research on the biopreservative capacity of C. maltaromaticum should be conducted with strain CM_827. [less ▲]

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See detailSensory quality of beef patties inoculated with strains of Carnobacterium maltaromaticum with potential as biopreservatives
Didimo Imazaki, Pedro Henrique ULiege; Jacques-Houssa, Charlotte ULiege; Kergourlay, Gilles ULiege et al

Poster (2015, August)

Biopreservation is the use of naturally occurring microorganisms and/or their inherent antimicrobial compounds to extend shelf life and to enhance the safety of foods. The aim of the present study was to ... [more ▼]

Biopreservation is the use of naturally occurring microorganisms and/or their inherent antimicrobial compounds to extend shelf life and to enhance the safety of foods. The aim of the present study was to perform a sensory evaluation of beef patties inoculated with strains of C. maltaromaticum with potential as biopreservatives. Three different strains of C. maltaromaticum (CM_824, CM_827 and CM_829) isolated from vacuum packaged beef with long shelf life were selected for this study. An untrained panel was requested to make a sensory evaluation of raw and cooked beef patties 8 and 10 days after inoculation with the selected strains at 104 and 106 UFC/g and storage in high O2 atmosphere. This preliminary study permitted to evaluate the effect of three C. maltaromaticum strains on the sensory quality of beef patties. Strain CM_827 did practically not change the sensory attributes of beef patties. Samples inoculated with strain CM_824 and CM_829 received the worst scores for several of the tested descriptors. Therefore, further research on the biopreservative capacity of C. maltaromaticum should be conducted with strain CM_827. [less ▲]

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See detailMorphological and functional characterization of Carnobacterium maltaromaticum isolated from vacuum-packed beef with long shelf life
Didimo Imazaki, Pedro Henrique ULiege; Tahiri, Assia ULiege; Ndedi Ekolo, François ULiege et al

Poster (2015, June 17)

Temperatures near the freezing point of meat (−1.5 °C), associated with vacuum packaging, allows the preservation of this product up to several months, which makes possible the meat trade across the ... [more ▼]

Temperatures near the freezing point of meat (−1.5 °C), associated with vacuum packaging, allows the preservation of this product up to several months, which makes possible the meat trade across the planet without resorting to freezing. Other the type of packaging and the storage temperature, the shelf-life of meat is directly related to its initial microbiological ecosystem and its evolution. Carnobacterium maltaromaticum is a lactic acid bacterium, and many lactic acid bacteria associated with meat are known for their bactericidal or bacteriostatic activity against other strains, species or genera of bacteria. In this way, the presence of certain lactic acid bacteria adapted to a low temperature in fresh meat could extend the shelf life and improve the microbial stability and safety of this product. The aim of this study was to perform a morphological and functional characterization of a C. maltaromaticum strain (lab. ref. CFAUS2/DLC/4/E1) with potential bioprotective effect isolated from commercial vacuum packaged long shelf life beef. The morphological, biochemical and enzymatic profiles, the influence of different temperatures and atmospheres, and the microbial stability of fresh beef inoculated with the C. maltaromaticum strain were evaluated. Morphological, biochemical and enzymatic profiles of the isolated C. maltaromaticum strain were similar to those of two reference strains (LMG 11393 and LMG 22902). The evaluation of the influence of different atmospheres showed that the growth of C. maltaromaticum was the slowest in an atmosphere containing 70 % O2 and 30 % CO2. Vacuum packaging is therefore more suitable for the growth of this bacterium. An antimicrobial effect against Enterobacteriaceae was highlighted on inoculated fresh meat. The functional characterization of this strain will be further pursued by genotypic characterization and its potential bioprotective effect will also be studied. [less ▲]

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See detailEffect of storage and cooking on the fatty acid profile of omega-3 enriched eggs and pork meat marketed in Belgium
Douny, Caroline ULiege; El Khoury, Rawad; Delmelle, Julien et al

in Food Science & Nutrition (2015), 3(2), 140-152

Detailed reference viewed: 85 (34 ULiège)
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See detailMeat composition and quality of young growing Belgian Blue bulls offered a fattening diet with selenium enriched cereals
Mehdi, Youcef ULiege; Clinquart, Antoine ULiege; Hornick, Jean-Luc ULiege et al

in Canadian Journal of Animal Science (2015), 95(3), 465-473

The objective of this study was to evaluate the effects of selenium (Se) enrichment of cereals on Belgian Blue bulls performance, on meat quality and chemical composition. Twenty three bulls were used in ... [more ▼]

The objective of this study was to evaluate the effects of selenium (Se) enrichment of cereals on Belgian Blue bulls performance, on meat quality and chemical composition. Twenty three bulls were used in the present study. Twelve bulls were offered a control diet containing Se at a basal concentration of 58 µg kg-1 of dry matter (DM) and the other 11 bulls given a diet containing 173 µg kg-1DM of Se by means of Se enriched spelt and barley. The Se enrichment of the diet did not affect either the growth performance, the slaughter data or meat quality (p>0.05). There were no effects of Se on tenderness, oxydative rancidity and water losses. However, there were some effects of Se enrichment on the meat chemical composition. The ether extract was decreased from 2.1 to 1.7% DM (p<0.05). There were also significant Se enrichment (p < 0.001) in Longissimus thoracis muscle (177 vs 477 ng g1-DM) and organs: liver (474 vs 1126 ng g1-DM) and kidney (4956 vs 5655 ng g1-DM), Under such condition, the human consumption of a piece of such meat or liver can cover a large part of the recommended daily Se intake estimated between 30 to 57%. [less ▲]

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See detailMicrobiological safety and quality aspects of the short supply chain: SWOT analysis of the Belgian case study
Verraes, Claire; Uyttendaele, Mieke; Clinquart, Antoine ULiege et al

in British Food Journal (2015), 117(9), 2250-2264

Purpose – In recent years consumers in Belgium have shown a great interest for foods from the short supply chain. The difference with the conventional chain is that in the short supply chain the primary ... [more ▼]

Purpose – In recent years consumers in Belgium have shown a great interest for foods from the short supply chain. The difference with the conventional chain is that in the short supply chain the primary products are locally processed and sold directly by the producer to the consumer. The short supply chain has different microbiological quality and safety aspects in comparison with the conventional chain. The purpose of this paper is to evaluate these aspects. Design/methodology/approach – The methodology consists of analyzing the available scientific literature and results of microbiological analyses on foods from the short supply chain. Findings – The main findings were that Listeria monocytogenes was frequently detected (15 percent) in sampled raw dairy products whereas Salmonella was not isolated in 1,023 samples. Human pathogenic vero (cyto) toxin-producing Escherichia coli and Campylobacter spp. are potential hazards, in particular for products that are not thermally treated. Data with regard to E. coli counts showed a greater variability in products from the short supply chain compared to the conventional chain. Research limitations/implications – The paper discusses strengths and weaknesses with impact on microbial quality and safety in operation of food safety management in the short supply chain vs the conventional chain. Originality/value – This is the first paper that assesses the risks from the short supply chain vs the conventional chain and that makes recommendations for operators in the short supply chain [less ▲]

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See detailThe impact of oregano (Origanum heracleoticum) essential oil and carvacrol on virulence gene transcription by Escherichia coli O157:H7
Mith, Hasika; Clinquart, Antoine ULiege; Zhiri, Abdesselam et al

in FEMS Microbiology Letters (2015), 362

The aim of the current study was to determine, via reverse transcription quantitative polymerase chain reaction (RT-qPCR) analysis, the effect of oregano essential oil (Origanum heracleoticum) and ... [more ▼]

The aim of the current study was to determine, via reverse transcription quantitative polymerase chain reaction (RT-qPCR) analysis, the effect of oregano essential oil (Origanum heracleoticum) and carvacrol, its major component, on the expression of virulence-associated genes in enterohaemorrhagic Escherichia coli (EHEC) O157:H7 ATCC strain 35150. Both oregano oil and carvacrol demonstrated their efficacy firstly, by inhibiting the transcription of the ler gene involved in up regulation of the LEE2, LEE3 and LEE4 promoters and of attaching and effacing lesions and secondly by decreasing both Shiga toxin and fliC genes expression. In addition, a decrease in luxS gene transcription involved in quorum sensing was observed. These results were dose dependent and showed a specific effect of O. heracleoticum and carvacrol in downregulating the expression of virulence genes in EHEC O157:H7. These findings suggest that oregano oil and carvacrol have the potential to mitigate the adverse health effects caused by virulence gene expression in EHEC O157: [less ▲]

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See detailImpact of aging technique, muscle and previous vacuum storage time on oxidative stability of beef packaged under high-oxygen atmosphere
Didimo Imazaki, Pedro Henrique ULiege; Douny, Caroline ULiege; Scippo, Marie-Louise ULiege et al

Conference (2014, December 11)

The aim of this study was to compare the effect of aging technique (wet-aging vs. carcass-aging), muscle (longissimus dorsi vs. rectus femoris) and previous vacuum storage time on color and lipid ... [more ▼]

The aim of this study was to compare the effect of aging technique (wet-aging vs. carcass-aging), muscle (longissimus dorsi vs. rectus femoris) and previous vacuum storage time on color and lipid stability of beef packaged in high-oxygen atmosphere. After a seven-day wet- or carcass-aging step, longissimus dorsi and rectus femoris muscle cuts from 4 Belgian Blue cows were vacuum packaged and stored at −1 °C for up to 28 days. At different times, part of these samples was repackaged under modified atmosphere – 70 % O2:30 % CO2 –, and stored during 7 days at +4 °C. The following parameters were evaluated at different intervals: color (CIE L*a*b*), metmyoglobin %, lipid oxidation (TBARS), antioxidant enzyme activities (catalase, glutathione peroxidase and superoxide dismutase), alpha-tocopherol and fat content. The sensitivity of modified atmosphere repacked meat cuts to oxidation was influenced by the conditions of the previous aging period (wet > carcass conditions), muscle (rectus femoris > longissimus dorsi) and length of the vacuum storage. Oxidation stability could be associated with catalase activity, and no association could be established with the alpha-tocopherol content. [less ▲]

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See detailStabilité oxydative des viandes blanc bleu belge conditionnées sous atmosphère riche en oxygène après maturation sous vide ou en carcasse
Didimo Imazaki, Pedro Henrique ULiege; Douny, Caroline ULiege; Scippo, Marie-Louise ULiege et al

in Viandes et Produits Carnés (2014, November)

The aim of this study was to evaluate the physico-chemical stability of Belgian Blue beef packaged under high-oxygen atmosphere as a function of a previous aging technique (wet-aging in vacuum conditions ... [more ▼]

The aim of this study was to evaluate the physico-chemical stability of Belgian Blue beef packaged under high-oxygen atmosphere as a function of a previous aging technique (wet-aging in vacuum conditions vs. carcass-aging). Two muscles (longissimus dorsi vs. rectus femoris) were studied. After a seven-day wet- or carcass-aging step, muscle cuts from 4 Belgian Blue cows were vacuum packaged and stored at −1 °C for up to 28 days. Each 14 days, part of these samples was repackaged under modified atmosphere (70 % O2:30 % CO2), and stored during 7 days at +4 °C. The following parameters were evaluated: color, metmyoglobin %, metmyoglobin reducing activity, fat content, fatty acid profile, lipid oxidation, antioxidant enzyme activities and alpha-tocopherol content. The sensitivity of modified atmosphere repacked meat cuts to oxidation was influenced by the conditions of the previous aging period (wet > carcass conditions), muscle (rectus femoris > longissimus dorsi) and length of the vacuum storage. [less ▲]

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See detailImpact of aging technique and muscle on oxidative stability of beef packaged under high-oxygen atmosphere
Didimo Imazaki, Pedro Henrique ULiege; Tahiri, Assia ULiege; Thimister, Jacqueline ULiege et al

Poster (2014, October 17)

Two common approaches for beef aging are wet-aging and carcass-aging. Wet-aging refers to meat aged in a sealed vacuum package at refrigerated temperatures, while carcass aged at controlled temperatures ... [more ▼]

Two common approaches for beef aging are wet-aging and carcass-aging. Wet-aging refers to meat aged in a sealed vacuum package at refrigerated temperatures, while carcass aged at controlled temperatures and humidity is defined as carcass-aging. Carcass-aging is an ancient process used nowadays to produce beef characterized by its superior quality. The meat conservability is influenced by its sensitivity to oxidative process which can vary from one muscle to another. The aim of this study was to compare the effect of aging technique (wet-aging vs. carcass-aging), muscle (longissimus dorsi vs. rectus femoris) and previous vacuum storage time on colour and lipid stability of beef packaged in high-oxygen atmosphere. After a seven-day wet- or carcass-aging step, longissimus dorsi and rectus femoris muscle cuts from 4 Belgian Blue cows were vacuum packaged and stored at −1 °C for up to 28 days. At different times, part of these samples was repackaged under modified atmosphere – 70 % O2:30 % CO2 –, and stored during 7 days at +4 °C in order to simulate retail conditions. The following parameters were evaluated: colour (CIE L*a*b*), metmyoglobin %, lipid oxidation (TBARS), antioxidant enzyme activities (catalase, glutathione peroxidase and superoxide dismutase), alpha-tocopherol and fat content. The sensitivity of high-oxygen atmosphere repacked meat cuts to oxidation was influenced by the aging technique (wet > carcass conditions), muscle (rectus femoris > longissimus dorsi) and length of the vacuum storage. Oxidation stability could be associated with muscle catalase activity, and no association could be established with the alpha-tocopherol content. [less ▲]

Detailed reference viewed: 21 (3 ULiège)
See detailThe effect of oregano (Origanum heracleoticum) essential oil and carvacrol on virulence gene transcription by Escherichia coli O157:H7
Mith, Hasika; Clinquart, Antoine ULiege; Zhiri, Abdesselam et al

Poster (2014, October 17)

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See detailEvaluation of morphological and functional characteristics of Carnobacterium maltaromaticum isolated from vacuum-packaged beef with long shelf life
Didimo Imazaki, Pedro Henrique ULiege; Tahiri, Assia ULiege; Ndedi Ekolo, François ULiege et al

Poster (2014, October 17)

Carnobacterium maltaromaticum is a lactic acid bacterium, and many lactic acid bacteria associated with meat are known for their bactericidal or bacteriostatic activity against other strains, species or ... [more ▼]

Carnobacterium maltaromaticum is a lactic acid bacterium, and many lactic acid bacteria associated with meat are known for their bactericidal or bacteriostatic activity against other strains, species or genera of bacteria. The presence of certain lactic acid bacteria adapted to a low temperature in fresh meat could extend the shelf life and improve the microbial stability and safety of this product. The aim of this study was to perform a morphological and functional characterization of a C. maltaromaticum strain with a potential bioprotective effect isolated from vacuum packaged beef with very long shelf life. The morphological, biochemical and enzymatic profiles, the influence of different temperatures and atmospheres, and the microbial stability of fresh beef inoculated with the C. maltaromaticum strain were evaluated. The isolated C. maltaromaticum strain presented similar morphological, biochemical and enzymatic profiles as those of two reference strains (LMG 11393 and LMG 22902). Among the studied conditions, a temperature of +12 °C and an atmosphere poor in oxygen were optimal for the growth of C. maltaromaticum. Vacuum packing is therefore suitable for this bacterium. An antimicrobial effect against Enterobacteriaceae was highlighted on inoculated fresh meat stored under N2. The functional characterization of this isolate will be further pursued by a genotypic characterization. Special attention will be taken to study its bioprotective properties. [less ▲]

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See detailCarnobacterium maltaromaticum isolated from vacuum-packed beef with long shelf life: morphological and functional characterization
Didimo Imazaki, Pedro Henrique ULiege; Tahiri, Assia ULiege; Ndedi Ekolo, François et al

Poster (2014, September)

This study was conducted as part of a research project on meat conservability, in particular vacuum packaged beef displaying very long shelf lives at a temperature close to the freezing point, and its ... [more ▼]

This study was conducted as part of a research project on meat conservability, in particular vacuum packaged beef displaying very long shelf lives at a temperature close to the freezing point, and its objective was to perform a morphological and functional characterization of C. maltaromaticum with potential bioprotective effect isolated from vacuum packaged long shelf life beef. The isolated C. maltaromaticum strain presented similar morphological, biochemical and enzymatic profiles as those of two reference strains (LMG 11393 and LMG 22902). Among the studied conditions, a temperature of +12 °C and an atmosphere poor in oxygen (100 % N2) were the optimal conditions for the growth of C. maltaromaticum. Nevertheless, growth on lower temperatures is also possible. After inoculation of beef samples with C. maltaromaticum and 7 days of storage under vacuum at −1 °C, no effect was observed on the total viable count and on the count of lactic acid bacteria. A reduction of Pseudomonas sp. and Brochothrix thermosphacta was observed during the first week of storage under vacuum conditions. After 7 days of subsequent storage of the same samples under modified atmosphere at +4 °C, the inoculant favored the growth of B. thermosphacta and inhibited the growth of Enterobacteriaceae under 100 % N2. No effect of the inoculant was observed when an atmosphere rich in oxygen was applied. The evaluation of the influence of different atmospheres showed that the growth of C. maltaromaticum was slower in an atmosphere containing O2 and CO2. Long-term storage under vacuum at low temperatures are therefore suitable for the growth of this bacterium. An antimicrobial effect against Enterobacteriaceae was highlighted on inoculated fresh meat stored under N2. The functional characterization of this strain will be further pursued by genotypic characterization. Special attention will be taken to study its bioprotective properties. [less ▲]

Detailed reference viewed: 78 (11 ULiège)