  Dialogue between Blastocyst hCG and Endometrial LH/hCG Receptor: Which Role in Implantation?PERRIER d'HAUTERIVE, Sophie  ; Berndt, Sarah ; Tsampalas, Marie et alin Gynecologic & Obstetric Investigation (2007), 64(3), 156-60 The specific interaction of blastocyst-derived human chorionic gonadotropin (hCG) and endometrial LH/hCG-R constitutes a fundamental component of the molecular dialogue at the materno-fetal interface ... [more ▼] The specific interaction of blastocyst-derived human chorionic gonadotropin (hCG) and endometrial LH/hCG-R constitutes a fundamental component of the molecular dialogue at the materno-fetal interface. From our observations and studies from other groups, hCG was indeed shown to play a significant role in implantation and tolerance of the embryo, decidual differentiation and remodeling, as well as in placentation. The profile pattern of LH/hCG-R expression by endometrial epithelium correlates with the theoretical timing of the implantation window. Studies are currently being conducted in assisted medical procreation and in an animal model of implantation to establish the index of LH/hCG-R expression as a new biomarker of uterine receptivity for embryo implantation. [less ▲] Detailed reference viewed: 47 (5 ULg) Du nouveau à l'interface materno-foetale: rôle du couple hCG/récepteur LH-hCG dans l'implantation embryonnairePERRIER d'HAUTERIVE, Sophie ; Charlet, Jeanne de Chantal ; Dubois, Michel et alin Revue Médicale de Liège (2006), 61(10), 705-12 Implantation of the embryo into the maternal endometrium represents a unique biological process, combining an immunological (tolerance of an allograft) and biological (adhesion of two epitheliums) paradox ... [more ▼] Implantation of the embryo into the maternal endometrium represents a unique biological process, combining an immunological (tolerance of an allograft) and biological (adhesion of two epitheliums) paradox. The success of implantation depends on a receptive endometrium, a functionally normal blastocyst and a synchronized cross-talk between embryonic and maternal tissues. Though sexual steroids control the process, a cascade of growth factors or cytokines are the prime paracrine mediators of the dialogue at the maternal-embryonic interface. HCG is one of the molecules most precociously produced by the embryo and is the most specific marker of its presence. HCG is a luteotropic factor which relays the inadequate support provided by the reduced rates of LH, but also influences the pregnancy on a paracrine mode by a local action on implantation process, probably by interacting with its receptor, the LH/hCG-R that we have evidenced on endometrial epithelium. We demonstrate that embryo actively participate into its implantation, tolerance and placentation. [less ▲] Detailed reference viewed: 57 (9 ULg)Human chorionic gonadotropin and growth factors at the embryonic-endometrial interface control leukemia inhibitory factor (LIF) and interleukin 6 (IL-6) secretion by human endometrial epitheliumPERRIER d'HAUTERIVE, Sophie ; Charlet, Jeanne de Chantal ; Berndt, Sarah et alin Human Reproduction (2004), 19(11), 2633-2643 BACKGROUND: The elucidation of the molecular mechanisms by which the embryo contributes to its implantation is an area of extensive research. The main objective of this study was to investigate the ... [more ▼] BACKGROUND: The elucidation of the molecular mechanisms by which the embryo contributes to its implantation is an area of extensive research. The main objective of this study was to investigate the pattern of leukemia inhibitory factor (LIF) and interleukin-6 (IL-6) secretion by human endometrial epithelium, and their regulation by human chorionic gonadotropin (hCG) and other growth factors present at the embryonic-endometrial interface. METHODS: Endometrial epithelial cells (EEC) were isolated from biopsies collected at both proliferative and secretory phases of fertile women. RESULTS: HCG (1-50 IU/ml) increased LIF secretion by EEC cultures derived from follicular phase (up to 285+/-75%) or from secretory phase (up to 212+/-16%). In contrast, hCG reduced IL-6 secretion by EEC in both phases. The hCG/LH receptor gene was transcribed by EEC as evidenced by RT-PCR. Insulin-like growth factors 1 and 2 increased LIF secretion by EEC. Transforming growth factor beta1 stimulated LIF and reduced IL-6 secretion. CONCLUSIONS: Through hCG, the blastocyst may be involved in the control of its implantation (via an increase of proimplantatory LIF) and tolerance (via an inhibition of proinflammatory IL-6). Other growth factors present at the embryonic-endometrial interface are also involved in the control of LIF and IL-6 endometrial secretion. [less ▲] Detailed reference viewed: 41 (8 ULg)La fenetre implantatoirePerrier D'Hauterive, Sophie ; Charlet, Jeanne de Chantal ; Goffin, Frédéric et alin Journal de Gynécologie, Obstétrique et Biologie de la Reproduction (2002), 31(5), 440-55 BACKGROUND: Embryo implantation is a complex event involving apposition followed by adhesion of the blastocyst to the maternal endometrium, and finally invasion of this endometrium. Though implantation ... [more ▼] BACKGROUND: Embryo implantation is a complex event involving apposition followed by adhesion of the blastocyst to the maternal endometrium, and finally invasion of this endometrium. Though implantation could occur in any human tissue, the endometrium is the only tissue where embryo implantation cannot occur except during a restricted period called the implantation window. During this window, the endometrium is highly receptive to the embryo. MATERIAL: and methods. We reviewed the literature concerning the different factors involved in improved endometrial receptivity and implantation. RESULTS: Maternal - embryo crosstalk is favored by the implantation window. Endometrial receptivity results from the acquisition of ligands or receptors facilitating apposition, then adhesion of the embryo, or from the loss of components preventing it. The molecular basis of the implantation window remains to be defined. CONCLUSION: Despite progress in assisted reproduction technologies, the lack of control of implantation remains a major obstacle to successful pregnancy. It is of prime importance to determine the characteristic features of a receptive endometrium and, among the many markers proposed by in vitro studies, to analyze in humans those demonstrated by knock-out experiments to play a crucial role in mice. [less ▲] Detailed reference viewed: 16 (3 ULg)Identification of neurotensin-related peptides in human thymic epithelial cell membranes and relationship with MHC class I molecules; ; et al in Journal of Neuroimmunology (1997), 76 This study shows the expression at the cell surface of human thymic epithelial cells (TEC) of a neurotensin (NT)-like immunoreactivity. NT radio-immunoassay (RIA) revealed that cultured human TEC contain ... [more ▼] This study shows the expression at the cell surface of human thymic epithelial cells (TEC) of a neurotensin (NT)-like immunoreactivity. NT radio-immunoassay (RIA) revealed that cultured human TEC contain ± 5 ng immunoreactive (ir) NT/106 cells, of which 5% is associated with plasma cell membranes. HPLC analysis of NT-ir present in human TEC showed a major peak of NT-ir corresponding to NT1–13. NT-ir was not detected in the supernatant of human TEC cultures. Using an affinity column prepared with a anti-MHC class I monoclonal antibody, NT-ir-related peptides were retained on the column and eluted together with MHC class I-related proteins. According to the elution time on HPLC of these peptides, they correspond to intact NT1–13, as well as to smaller fragments of NT1–13. [less ▲] Detailed reference viewed: 23 (2 ULg) |
||
