References of "Chalghoumi, Raja"
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See detailPotential of Dried Egg Yolk as a Source of Immunoglobulins to Control Salmonella in the Gastrointestinal Tract of the Broiler Chicken
Marcq, Christopher ULg; Portetelle, Daniel ULg; Chalghoumi, RAJA ULg et al

in 17th European Symposium on Poultry Nutrition : Proceedings & Abstracts (2009, August)

Egg yolk immunoglobulins (IgY) are considered as a promising tool of establishing passive immunity against enteric pathogens (e.g. Salmonella) in poultry (Kovacs-Nolan and Mine, 2005). In our previous ... [more ▼]

Egg yolk immunoglobulins (IgY) are considered as a promising tool of establishing passive immunity against enteric pathogens (e.g. Salmonella) in poultry (Kovacs-Nolan and Mine, 2005). In our previous works, in-feed incorporation of freeze-dried yolk powder (FYP) improved performances of Salmonella-infected broilers (Marcq et al., 2009). Nevertheless, no extra value could be attributed to the presence of specific IgY when a comparison was done between impact of hyperimmune or non immune FYP both concerning growth performances and Salmonella infection. We hypothesized that these inconclusive in vivo results were related to the sensitivity of IgY to gastrointestinal conditions. Egg yolks with high IgY titers directed against Salmonella Enteritidis and Typhimurium were obtained from laying hens immunized as previously described (Chalghoumi et al., 2008). Three feed additives derived from these eggs were produced: FYP, spray-dried yolk powder (SYP), and freeze-dried water-soluble fraction of yolk powder (WSFP). A force-feeding assay was realized on these additives with 48 five-week-old males Ross broiler chicken (Salmonella spp.-free status). Survival of undigested IgY and bioactive Fab fragments was evaluated in the intestinal tract by ELISA. Statistical analyses were done using GLM procedure of Minitab 15.1. Software. Differences were considered significant when P<0.05. As a result of the digestive process, the levels of total and specific undigested IgY found throughout the intestine were dramatically reduced for the three additives. For FYP, no difference was observed with intestinal contents of control animals (P>0.05) and for WSFP, which correspond to semi-purified IgY, the immunological activity reached the same level than that for SYP (P>0.05) whereas its initial level was almost ten times higher. The results suggest a protective effect of whole yolk on IgY with SYP but such effect was not observed with FYP. High levels of Fab fragments were identified in intestinal contents, highlighting the importance of protein digestion in the digestive tract and especially pepsin digestion. These Fab fragments conserve their immunological functionality and remained detectable in all intestinal segments including the caecum which represents the major site of Salmonella infection in poultry. These results can explain our previous challenge data and reveal that a protection treatment should be applied to dried egg yolk to improve the resistance of IgY to gastrointestinal conditions in broilers. [less ▲]

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See detailDéveloppement d’une stratégie d’immunisation passive du poulet de chair vis-à-vis de Salmonella Enteritidis et Typhimurium à l’aide d’anticorps du jaune d’œuf
Marcq, Christopher ULg; Chalghoumi, RAJA ULg; Beckers, Yves ULg et al

in 8èmes JRA : Synthèses et textes (2009)

The present study investigates the potential of oral immunotherapy using hen egg yolk immunoglobulins (IgY) as a strategy to reduce the impact of Salmonella contamination on broilers. In a first step ... [more ▼]

The present study investigates the potential of oral immunotherapy using hen egg yolk immunoglobulins (IgY) as a strategy to reduce the impact of Salmonella contamination on broilers. In a first step, hyperimmune eggs were obtained from laying hens immunized using Salmonella Enteritidis (SE) and Typhimurium (ST) outer membrane proteins in emulsion with Freund adjuvants. This led to the production of high levels of IgY antibodies directed against the two Salmonella serovars in egg yolks. Furthermore, no adverse affects were detected on mortality or laying rate in hens injected with this vaccine. Powders were obtained from these eggs by spray- or freeze-drying the whole yolk. In order to study the effect of these powders on broiler’s growth performances, a challenge trial was conducted. 384 Salmonella spp.-free day-of-hatch chickens received supplemented feed (5 % freeze-dried egg yolk powder) since arrival, and were infected at day four with 106 cfu of SE and ST per animal. We found a significant improvement in growth performances for the groups receiving the yolk-enriched diets. Nevertheless, none of the supplement concentrations allowed to raise the same body weight after 28 days of complementation than in uninfected broilers (p < 0,05). Interestingly, nonimmune egg yolk powder also exhibited a positive effect on performances of broilers experimentally infected with SE and ST. Our results suggest that passive immunization through egg yolk powders could be useful to reduce negative effects of Salmonella infection on broilers growth performances. [less ▲]

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See detailProduction Of Hen Egg Yolk Immunoglobulins Simultaneously Directed Against Salmonella Enteritidis And Salmonella Typhimurium In The Same Egg Yolk
Chalghoumi, Raja ULg; Thewis, André ULg; Portetelle, Daniel ULg et al

in Poultry Science (2008), 87(1), 32-40

The present study was an attempt to raise hen egg yolk Ig (IgY) simultaneously directed against Salmonella Enteritidis (SE) and Salmonella Typhimurium (ST) in the same egg yolk. The immunopotentiating ... [more ▼]

The present study was an attempt to raise hen egg yolk Ig (IgY) simultaneously directed against Salmonella Enteritidis (SE) and Salmonella Typhimurium (ST) in the same egg yolk. The immunopotentiating effect of 2 different adjuvants—Freund’s adjuvants (FA) and immunostimulating complexes matrix (IM)—on antibody response was also evaluated. Bacterial outer membrane proteins (OMP) were selected as target antigens. The ISA Brown hens, specific-Salmonella spp.-free status, divided into 6 groups were intramuscularly injected with a monocompound antigen preparation: SE-OMP (treatment SEFA or SE-IM) or ST-OMP (treatment ST-FA or ST-IM), or a combined antigen preparation: ¹⁄₂ SE-OMP and ¹⁄₂ STOMP (treatment SEST-FA or SEST-IM). Titers of antibodies in yolk were evaluated biweekly with ELISA. There was no antigen × adjuvant interaction on antibody titers. Anti-SE IgY titers in hens that received treatment SESTFA or SEST-IM were statistically similar (P > 0.05) as compared with those obtained from hens immunized with treatment SE-FA or SE-IM. Anti-ST IgY titers in hens immunized with treatment SEST-FA or SEST-IM were slightly lower than those of hens that received treatment ST-FA or ST-IM. The cross-reactivity of anti-SE IgY, induced by treatment SE-FA or SE-IM, with ST-OMP antigen and that of anti-ST IgY, induced by ST-FA or ST-IM, with SE-OMP antigen were arbitrarily assessed on d 43 and 155 by ELISA. The average cross-reactivity of anti-SE IgY with ST-OMP antigen was 71.7%. The average cross-reactivity of anti-ST IgY with SE-OMP antigen was 78.8%. In FA groups, antibody titers were found higher (P < 0.05) than those in IM groups. Furthermore, no extensive lesions or clinical abnormalities were detected in hens injected with FA. These findings showed the opportunity to raise IgY antibody against 2 Salmonella serovars in the same yolk and that FA was more efficient than IM in mediating antibody response. [less ▲]

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