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See detailFermentation of date palm juice by curdlan gum production from Rhizobium radiobacter ATCC 6466 (TM): Purification, rheological and physico-chemical characterization
Ben Salah, Riadh; Jaouadi, Bassem; Bouaziz, Amin et al

in Lwt-Food Science And Technology (2011), 44(4), 1026-1034

The present study was undertaken to investigate the possibility of using date palm juice byproducts for curdlan production by Rhizobium radiobacter ATCC 6466(TM) in batch experiments. A number of ... [more ▼]

The present study was undertaken to investigate the possibility of using date palm juice byproducts for curdlan production by Rhizobium radiobacter ATCC 6466(TM) in batch experiments. A number of operational parameters, namely pH value, temperature range, inoculum ratio, agitation speed, carbon concentration, nitrogen source, and fermentation time, were investigated in terms of their optimal values for as well as individual and synergistic effects on curdlan production. The findings indicated that the strain exhibited a high ability to use the natural substrate under investigation. A curdlan production yield of 22.83 g/l was obtained in 500-ml agitated flasks (50 ml) when the strain was cultivated in the optimal medium (pH, 7; ammonium sulphate concentration, 2 g/l; date glucose juice concentration, 120 g/l) operating at 30 degrees C with an inoculum ratio of 5 ml/100 ml, an agitation speed of 180 rpm, and a fermentation period of 51 h. The purified date byproducts-curdlan (DBP-curdlan) had a molecular weight of 180 kDa, a linear structure composed exclusively of beta-(1,3)-glucosidic linkages, a melting temperature (T(m)) and glass transition temperature (T(g)) of 1.24 and -3.55 degrees C. respectively. The average measured heights of its molecules were noted to fluctuate between 14.1 +/- 0.07 and 211.73 +/- 0.6 mu m. (C) 2010 Elsevier Ltd. All rights reserved. [less ▲]

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See detailPRODUCTION OF XANTHAN GUM FROM XANTHOMONAS CAMPESTRIS NRRL B-1459 BY FERMENTATION OF DATE JUICE PALM BY-PRODUCTS (PHOENIX DACTYLIFERA L.)
Ben Salah, Riadh; Chaari, Kacem; Besbes, Souhail et al

in Journal of Food Process Engineering (2011), 34(2), 457-474

In this work, we studied the possibility to use date juice for xanthan gum production by Xanthomonas campestris NRRL B-1459. The results showed that this strain has a high ability to metabolize date juice ... [more ▼]

In this work, we studied the possibility to use date juice for xanthan gum production by Xanthomonas campestris NRRL B-1459. The results showed that this strain has a high ability to metabolize date juice. The data on optimization of physiological conditions of fermentation, pH, temperature, inoculum's size, glucose and nitrogen concentration showed that the maximum xanthan yield of 24.5 g/L was obtained from 60 g/L glucose, 3 g/L ammonium sulphate when batch fermentation was carried with 5% inoculum's size at pH 7, 28C, 48 h, on a rotary orbital shaker at 180 rpm. The polysaccharide purified by high performance liquid chromatography and analyzed by thin layer chromatography contained glucose, glucoronic acid and mannose. PRACTICAL APPLICATIONS Agri-food by-products rich in sugars may be used to produce high added value food ingredients such as xanthan gum. No work has studied the production of this polysaccharide from date. This study is focused on given value addition to date by-product (hard texture) by production of xanthan gum. The effect of several parameters (pH, temperature, inoculum's size, agitation speed, nitrogen source and carbon concentration) on production yield was investigated. [less ▲]

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See detailOptimisation of xanthan gum production by palm date (Phoenix dactylifera L.) juice by-products using response surface methodology
Ben Salah, Riadh; Chaari, Kacem; Besbes, Souhail et al

in Food Chemistry (2010), 121(2), 627-633

The present study was undertaken investigate and optimise the possibility of xanthan gum production by Xanthomonas campestris NRRL B-1459 in batch experiments on date palm juice by-products. Using an ... [more ▼]

The present study was undertaken investigate and optimise the possibility of xanthan gum production by Xanthomonas campestris NRRL B-1459 in batch experiments on date palm juice by-products. Using an experimental Response Surface Methodology complemented with a Central Composite Orthogonal Design, three major independent variables (date juice carbon source, nitrogen source and temperature) were evaluated for their individual and interactive effects on biomass and xanthan gum production. The optimal conditions selected were: 84.68 g/l for carbon source, 2.7 g/l for nitrogen source, and 30.1 degrees C for temperature. The experimental value obtained for xanthan production under these conditions was about 43.35 g/l, which was close to the 42.96 g/l value predicted by the model. Higher yields of biomass production could be obtained at 46.68 g/l for carbon source, 4.58 g/l for nitrogen source and 30 degrees C for temperature. The maximum value obtained for biomass production was 3.35 g/l, which was higher than the 2.98 g/l value predicted by the model. The xanthan formed was subjected to HPLC and TLC analyses and its molecular weight as well as pyruvate content were identified. The findings indicated that this polysaccharide contained glucose, glucoronic acid and mannose. Overall, the date palm juice by-products presented in the current study seem to exhibit promising properties that can open new pathways for the production of efficient and cost-effective xanthan gum. (C) 2010 Elsevier Ltd. All rights reserved. [less ▲]

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See detailRheological and physical properties of date juice palm by-product (phoenix dactylifera L.) and commercial xanthan gums.
Ben Salah, Riadh; Besbes, Souhail; Chaari, Kacem et al

in Journal of Texture Studies (2010), 41(2), 125-138

Rheological and thermal properties of xanthan produced by Xanthomonas campestris from date by-products (DBP-xanthan) and commercial xanthan were determined. DBP-xanthan gave solutions with lower apparent ... [more ▼]

Rheological and thermal properties of xanthan produced by Xanthomonas campestris from date by-products (DBP-xanthan) and commercial xanthan were determined. DBP-xanthan gave solutions with lower apparent viscosity than commercial xanthan. However, DBP-xanthan solutions were more stable to temperature changes and ionic strength than commercial xanthan. Gradual increase of storage modulus (G') as function of frequency was observed for the two polysaccharides. On the other hand, loss modulus (G ') remained constant for DBP-xanthan and an increase was observed for commercial xanthan. Granulometric profile indicates that DPB-xanthan particle size was lower than commercial xanthan. Differential scanning calorimetry showed that the melting temperatures (T-m) and enthalpy (Delta H-m) of DBP-xanthan were higher than those of commercial xanthan. These properties of DBP-xanthan are quite rare among xanthan described in the literature and give this new gum great potential for use in the field of bioindustries as thickening and stabilizing agents. PRACTICAL APPLICATIONS Xanthan gum from date by-products (DBP-xanthan) was recently produced in our laboratory. No work has been undertaken to study the rheological properties of this polysaccharide to date. Rheological and some physicals properties were compared to those of commercial xanthan. This could promote industrial use of DBPs as low-cost natural source for xanthan gum production. [less ▲]

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