References of "CARLISI, Ignazia"
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See detailIDS iSYS automated intact procollagen-1-Nterminus pro-peptide assay: method evaluation and reference intervals in adults and children
Morovat, Alireza; Catchpole, Anthony; MEURISSE, Angélique ULg et al

in Clinical Chemistry & Laboratory Medicine (2013), 51(10), 2009-2018

Background: We carried out a technical evaluation of the Immunodiagnostic Systems (IDS) automated intact procollagen- I N-terminus propeptide (PINP) assay on the iSYS platform, and established reference ... [more ▼]

Background: We carried out a technical evaluation of the Immunodiagnostic Systems (IDS) automated intact procollagen- I N-terminus propeptide (PINP) assay on the iSYS platform, and established reference intervals for PINP in both adults and children. Methods: Assay imprecision, recovery and interference were studied. Serum and plasma values were compared, and PINP stability was assessed. Using 828 specimens, IDS iSYS intact PINP and Roche E170 total PINP values were compared. Specimens from 597 adults and 485 children and adolescents were used to establish reference intervals for intact PINP. Results: The method demonstrated good recovery and acceptable imprecision. The assay was unaffected by icterus and lipaemia, but haemolysis decreased measured PINP. Serum and plasma values were comparable. There was a non-linear relation between IDS intact and Roche total PINP values. Pre- and post-menopausal women had comparable PINP values, but there was a difference between women of different age groups. Serum PINP in men showed a decline in young age up to 45 years, but remained steady thereafter. Separate reference intervals were established for four age groups in women and for two age groups in men. Data for children were partitioned into four-year age groups, and these showed PINP to be high with no major gender differences until 12 years of age. Thereafter, values in females decreased in 13–16 years age groups and further in 17–20 years age groups, whereas PINP increased in boys of 13–16 years of age with a subsequent decline at 17–20 years. Conclusions: The IDS iSYS PINP intact assay appears to be reliable. We have established gender- and age-related reference intervals for children and adults based on a relatively large healthy North European population. [less ▲]

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See detailTechnical and clinical evaluation of the VITROS Immunodiagnostic Products 25-OH Vitamin D Total Assay - comparison with marketed automated immunoassays and a liquid chromatography - tandem mass spectrometry method
CAVALIER, Etienne ULg; ROUSSELLE, Olivier ULg; FERRANTE, Nunzio ULg et al

in Clinical Chemistry & Laboratory Medicine (2013), 51(10), 1983-1989

Background: The study was conducted to evaluate the technical and clinical performance of the VITROS ® Immunodiagnostic Products 25-OH Vitamin D Total Assay, and compare it with the performance of five ... [more ▼]

Background: The study was conducted to evaluate the technical and clinical performance of the VITROS ® Immunodiagnostic Products 25-OH Vitamin D Total Assay, and compare it with the performance of five marketed automated assays and a liquid chromatography/mass spectrometry reference method (LC-MS/MS). Methods: Three hundred patient serum samples were used to compare the correlation of the VITROS ® 25-OH Vitamin D Total Assay with both the other immunoassays and the LC-MS/MS method, using Passing-Bablok regression and Bland-Altman analyses. Concordance of the diagnosis of vitamin D status was calculated to test the agreement between the different assays. In addition, samples containing vitamin D2 were used to test the assay ’ s ability to detect the D2 form of the vitamin. Results and conclusions: These results from the VITROS ® 5-OH Vitamin D Total Assay generally correlated well with those from most of the marketed immunoassays. Cross-reactivity of the D2 form was calculated as being close to 100%. Additionally, we found substantial variability in performance amongst the various assays, which suggests the need for optimisation and recalibration of commercial methods. [less ▲]

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See detailPerformance of the IDS-iSYS 1,25-dihydroxy vitamin D assay
GADISSEUR, Romy ULg; LUKAS, Pierre ULg; CARLISI, Ignazia ULg et al

Poster (2013, July)

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See detailEvaluation of the third-generation to second-generation PTH ratio on the Liaison XL (DIASORIN) as a marker for parathyroïd carcinoma
Schleck, Marie-Louise; LAURENT, Terry ULg; GADISSEUR, Romy ULg et al

in Biochimica Clinica (2013, May), 37(SS), 161

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See detailEvaluation of the performance of the new automated IDS-ISYS 1,25-dihydroxy vitamin D assay
GADISSEUR, Romy ULg; LUKAS, Pierre ULg; CARLISI, Ignazia ULg et al

in Biochimica Clinica (2013, May), 37(SS), 498

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