References of "Brunelle, Alain"
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See detailImpact of rhizosphere factors on cyclic lipopeptide signature from the plant beneficial strain Bacillus amyloliquefaciens S499
Nihorimbere, Venant; Cawoy, Hélène ULg; Seyer, Alexandre et al

in FEMS Microbiology Ecology (2012), 79

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See detailMultiple changes in peptide and lipid expression associated with regeneration in the nervous system of the medicinal leech
Mériaux, Céline; Arafah, Karim; Tasiemski, Aurélie et al

in PLoS ONE (2011), 6(4), 18359

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See detailMass spectrometry imaging of rat brain sections: nanomolar sensitivity with MALDI versus nanometer resolution by TOF–SIMS
Benabdellah, Farida; Seyer, Alexandre; Quinton, Loïc ULg et al

in Analytical and Bioanalytical Chemistry (2010), 396(1), 151-162

Mass spectrometry imaging is becoming a more and more widely used method for chemical mapping of organic and inorganic compounds from various surfaces, especially tissue sections. Two main different ... [more ▼]

Mass spectrometry imaging is becoming a more and more widely used method for chemical mapping of organic and inorganic compounds from various surfaces, especially tissue sections. Two main different techniques are now available: matrix-assisted laser desorption/ionizaton, where the sample, preliminary coated by an organic matrix, is analyzed by a UV laser beam; and secondary ion mass spectrometry, for which the target is directly submitted to a focused ion beam. Both techniques revealed excellent performances for lipid mapping of tissue surfaces. This article will discuss similarities, differences, and specificities of ion images generated by these two techniques in terms of sample preparation, sensitivity, ultimate spatial resolution, and structural analysis. [less ▲]

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See detailChemical Imaging on Liver Steatosis Using Synchrotron Infrared and ToF-SIMS Microspectroscopies
Le Naour, François; Bralet, Marie-Pierre; Debois, Delphine ULg et al

in PLoS ONE (2009), 4(10), 7408

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See detailIn Situ Lipidomic Analysis of Nonalcoholic Fatty Liver by Cluster TOF-SIMS Imaging
Debois, Delphine ULg; Bralet, Marie-Pierre; Le Naour, François et al

in Analytical Chemistry (2009), 81(8), 2823-2831

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See detailIn situ localisation and quantification of surfactins in a Bacillus subtilis swarming community by imaging mass spectrometry
Debois, Delphine ULg; Hamzé, Kassem; Guérineau, Vincent et al

in Proteomics (2008), 8(18), 3682-3691

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See detailThe patinas of the DogoneTellem statuary: A new vision through physico-chemical analyses
Mazel, Vincent; Richardin, Pascale; Debois, Delphine ULg et al

in Journal of Cultural Heritage (2008), 9

Numerous African art objects collected in the course of colonial or ethnological expeditions during the 20th century are partially or completely covered with a so-called ‘‘patina’’. These patinas have ... [more ▼]

Numerous African art objects collected in the course of colonial or ethnological expeditions during the 20th century are partially or completely covered with a so-called ‘‘patina’’. These patinas have been formed during religious and ritual ceremonies, where different substances have been spread out at the surface of the objects. The anthropomorphic statuettes from the Dogon culture are well-known examples of this kind of practice. A better understanding of the chemical composition of these patinas could explain details of the ceremonial practices realized throughout the centuries. An important challenge is to extract unequivocal information about the original constituents of these patinas, and the techniques used for their application on ritual objects. The Dogon statuary can be divided into three periods. The oldest one, before 1350 A.D., is called the Tellem period, according to the name of the people who lived in this area before the arrival of the Dogon. The most recent, after 1650 A.D., is the Dogon period. These two periods are separated by an intermediary period, where the attribution to the Tellem or to the Dogon cultures is difficult. During our study, we have worked on the patina of 12 Dogon artifacts from these three different periods. In this paper, we present the results obtained for two representative objects, one of the Tellem period and one of the Dogon period. The aim is to access the chemical composition of the patinas and to see if they can be compared to ethnological reports, as is often done in the literature on ethnological studies. Chemical imaging techniques, based on the combination of microscopy and spectroscopy, are well suited to study both the composition and the spatial organization of heterogeneous complex mixtures of organic and inorganic matter. Time-of-flight secondary ion mass spectrometry (ToF-SIMS), followed by scanning electron microscopy with energy dispersive X-ray analysis (SEM-EDS), and synchrotron radiation-based Fourier transform infrared microscopy (SR-mFTIR) have been applied to non-destructive analyses of micro-samples of the patinas of several Dogon statuettes. A very careful preparation, using ultramicrotomy on embedded samples, allowed us to perform successively all these measurements on a single fragment. Comparison and superposition of the different chemical images lead us to identify minerals (clays, quartz and calcium carbonate), and different organic products (proteins, starch, lipids), as well as to map their spatial distribution. The patina of the most recent statuettes presents a stratigraphy which can be related to successive uses of the objects for ritual purposes, and the results are in agreement with the ethnological reports. For the Tellem objects, the significant quantity of minerals and the lack of stratigraphy suggest that the patina was deposited according to a different recipe. This difference suggests the existence of a diversity of rituals that are more extensive than that described by the ethnologists. [less ▲]

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See detailPores formation on cell membranes by hederacolchiside A1 leads to a rapid release of proteins for cytosolic subproteome analysis
Mazzucchelli, Gabriel ULg; Cellier, Nicolas A; Mshviladzade, Vakhtang et al

in Journal of Proteome Research (2008), 7(4), 1683-1692

Hederacolchiside A1 was used to progressively permeabilize the membrane of human melanoma MEL-5 cells. Holes formation was followed by Scanning Electron Microscopy and interaction of the saponin with ... [more ▼]

Hederacolchiside A1 was used to progressively permeabilize the membrane of human melanoma MEL-5 cells. Holes formation was followed by Scanning Electron Microscopy and interaction of the saponin with cholesterol and phospholipids by TOF-SIMS. 2D-LC-MS/MS and 2D-SDS-PAGE show that the release of soluble proteins into serum-free culture media increases with time. This can lead to a new rapid and efficient strategy to analyze the cytosolic subproteome and it opens the door to get information from the cytosolic compartment for clinical proteomic studies. [less ▲]

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See detailIdentification of Ritual Blood in African Artifacts Using TOF-SIMS and Synchrotron Radiation Microspectroscopies
Mazel, Vincent; Debois, Delphine ULg; Touboul, David et al

in Analytical Chemistry (2007), 79(24), 9253-9260

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See detailAttempts for molecular depth profiling directly on a rat brain tissue section using fullerene and bismuth cluster ion beams
Debois, Delphine ULg; Brunelle, Alain; Laprévote, Olivier

in International Journal of Mass Spectrometry (2007), 260(2-3), 115-120

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