References of "Bozukova, Dimitriya"
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See detailBiointerface multiparametric investigation of intraocular lens acrylic materials
Bertrand, Virginie ULg; Bozukova, Dimitriya; Svaldo Lanero, Tiziana et al

in Journal of Cataract & Refractive Surgery (2014), 40(9), 1536-1544

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See detailBiointerface multiparametric study of intraocular lens acrylic materials.
Bertrand, Virginie ULg; Bozukova, Dimitriya; Svaldo Lanero, Tiziana et al

in Journal of cataract and refractive surgery (2014), 40(9), 1536-44

PURPOSE: To compare hydrophilic and hydrophobic acrylic materials designed for intraocular lenses in a multiparametric investigation in a liquid environment to highlight their properties in terms of ... [more ▼]

PURPOSE: To compare hydrophilic and hydrophobic acrylic materials designed for intraocular lenses in a multiparametric investigation in a liquid environment to highlight their properties in terms of adhesion forces, lens epithelial cell (LEC) adhesion, and tissue response as indicators of the risk for posterior capsule opacification (PCO) development. SETTING: University of Liege, Liege, Belgium. DESIGN: Experimental study. METHODS: The hydrophobicity and surface adhesion force were assessed using contact-angle and atomic force microscopy measurements. The bioadhesiveness of the disks and the tissue response were determined by in vitro experiments using bovine serum albumin and porcine LECs and by in vivo rabbit subcutaneous implantation, respectively. RESULTS: Increasing surface hydrophobicity led to a greater surface-adhesion force and greater LEC adhesion. After 1 month, the rabbit subcutaneous implants showed a similar thin layer of fibrous capsule surrounding the disks without extensive inflammation. A layer of rounded cells in contact with disks was detected on the hydrophobic samples only. CONCLUSIONS: Hydrophobic acrylic disks that have been associated with a reduced risk for PCO in clinical studies showed increased tackiness. FINANCIAL DISCLOSURES: Proprietary or commercial disclosures are listed after the references. [less ▲]

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See detailBioactive Intraocular Lens – A Strategy to Control Secondary Cataract
Huang, Yi-Shiang ULg; Bertrand, Virginie ULg; Bozukova, Dimitriya et al

in IFMBE Proceedings (2014), 41

Cataract is the opacity of the lens, causing impairment of vision or even blindness. Today, a surgery is still the only available treatment. The intraocular lens (IOL) is a polymer implant designed to ... [more ▼]

Cataract is the opacity of the lens, causing impairment of vision or even blindness. Today, a surgery is still the only available treatment. The intraocular lens (IOL) is a polymer implant designed to replace the natural lens in the cataract surgery. However, the bioinert materials could not satisfy the unmet need in the secondary cataract control. Posterior capsular opacification (PCO, or Secondary Cataract), characterized by a thick and cloudy layer of lens epithelial cells (LECs), is the most common postoperative complication. In our research, a bioactive molecule is immobilized onto the conventional acrylic hydrophilic polymer pHEMA (Poly(2-hydroxyethyl methacrylate)) using oxygen plasma treatment followed by deposition. The RGD peptide sequence, being well-known for its ability to promote cellular attachment by binding to integrin receptors, is designed to stimulate the adhesion of LECs on the IOL. Our data show the peptide immobilized biomaterial not only exhibits similar optical property, but also reveals enhanced biological properties in cell adhesion and cell morphology maintenance. By means of surface functionalization of IOL to stimulate LECs adhesion, the secondary cataract could be controlled. [less ▲]

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See detailPolymer based intraocular lens adsorbome: a bottom up proteomics study
Bertrand, Virginie ULg; Huang, Yi-Shiang ULg; Mazzucchelli, Gabriel ULg et al

Conference (2013, September 08)

In the present work an optimized sample preparation protocol to identify and quantify the “adsorbomes” of hydrophilic and hydrophobic materials for IOLs known to have a higher or a lower incidence of PCO ... [more ▼]

In the present work an optimized sample preparation protocol to identify and quantify the “adsorbomes” of hydrophilic and hydrophobic materials for IOLs known to have a higher or a lower incidence of PCO, respectively was obtained. [less ▲]

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See detailIntraocular lenses with functionalized surfaces by biomolecules in relation with lens epithelial cell adhesion
Huang, Yi-Shiang ULg; Alexandre, Michaël ULg; Bozukova, Dimitriya et al

Poster (2013, April 25)

A cataract is pathology opacity of the lens or capsule of the eye, causing impairment of vision or even blindness. Surgery, with lens extraction and intraocular lens implantation, is still the only ... [more ▼]

A cataract is pathology opacity of the lens or capsule of the eye, causing impairment of vision or even blindness. Surgery, with lens extraction and intraocular lens implantation, is still the only currently available treatment. The most common complication after implantation of intraocular lenses (IOLs) is the posterior capsular opacification (PCO) or secondary cataract. This is the result of lens epithelial cells (LECs) proliferation and their transition to mesenchymal cells. In 1997, a Sandwich theory was proposed to elucidate the developmental process of PCO. [1] According to this model, an IOL with higher affinity to LECs will induce a less PCO. In our research, the pHEMA (Poly(2-hydroxyethyl methacrylate)) based acrylic hydrophilic polymer is subjected to the surface modification by conjugating with the bioactive peptides. The RGD sequence, known for its excellent biocompatibility, is designed to stimulate the biointegration between the LECs and the polymer implant. [2]. From our research, The RGD peptide immobilized onto pHEMA surfaces significantly facilitates the adhesion of the porcine LEC. The peptide immobilized surface retains its biological function even after 10 times of autoclave. On the other hand, the immobilized peptide does not alter the hydrophobicity of the surface, the light transmission, as well as the cytotoxicity of the material. This functionalized biomaterial would possibly prevent the formation of PCO. [1] J Cataract Refract Surg. 1997 Dec;23(10):1539-42 [2] Trends Biotechnol. 2008 Jul;26(7):382-92 [less ▲]

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See detailA fast and facile synthetic route toward the preparation of nanoparticles of polythiophene and its derivatives
Bozukova, Dimitriya; Jérôme, Robert ULg; Jérôme, Christine ULg

in Journal of Nanoparticle Research (2013), 15(4), 15831-18

A novel photochemical water-based approach for the preparation of nanoparticles of polymerized thiophene (Th), thiophene methanol (ThM), or their mixtures (Th-co/or-ThM) was developed. The influence of 3 ... [more ▼]

A novel photochemical water-based approach for the preparation of nanoparticles of polymerized thiophene (Th), thiophene methanol (ThM), or their mixtures (Th-co/or-ThM) was developed. The influence of 3-[(2-acryloyloxy)methyl] thiophene (ATh) as cross-link agent on the stability of the nanoparticles and on their performances was investigated. The occurrence of a polymerization process was confirmed by Fourier transform infrared (FT-IR), UV and fluorescence emission spectroscopies. Nanoparticles with very narrow size distribution (0.0092–0.105 ATh cross-linked, 0.0635–0.272 uncross-linked) and ideal spherical shape (radius 35–47 nm ATh cross-linked, 49–109 nm uncross-linked) were obtained whatever the reaction composition. The size of the particles was found to depend strongly on the level of ATh-stabilization and to diminish upon increase of the ATh content. In contrast, in the same order, their thermal stability shifted toward higher temperatures. Thermal decomposition of the nanoparticles led to formation of carbon-nanoobjects, as observed by transmission electron microscopy, FT-IR, and RAMAN spectroscopies. The re-dispersibility of the dry Th-co/or-ThM nanoparticles in some conventional monomers and solvents has been estimated. [less ▲]

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See detailBiomechanical and optical properties of 2 new hydrophobic platforms for intraocular lenses
Bozukova, Dimitriya; Pagnoulle, Christophe; Jérôme, Christine ULg

in Journal of Cataract & Refractive Surgery (2013), 39(9), 1404-1414

Purpose: To compare the biomechanical and optical properties of 2 new hydrophobic platforms and a series of commercially available foldable intraocular lenses (IOLs). Results: With 1 exception, IOLs ... [more ▼]

Purpose: To compare the biomechanical and optical properties of 2 new hydrophobic platforms and a series of commercially available foldable intraocular lenses (IOLs). Results: With 1 exception, IOLs equilibrated in aqueous medium had a lower glass-transition temperature, higher deformability, lower injection forces, and complete recovery of their initial optical properties after injection. Typical hydrophobic acrylic dry-packaged IOLs required higher injection forces with high residual deformation and lost part of their initial optical quality after injection. Hydrophobic acrylic C-loop, double C-loop, and closed quadripod haptics applied optimum compression forces to the capsular bag with negligible optic axial displacement and tilt compared with plate haptics and poly(methyl methacrylate) haptics. Conclusions: The combination of the C-loop haptic and the bioadhesive glistening-free material, which absorbs a predetermined amount of water, allowed for a biomechanically stable IOL. The same material used in association with a double C-loop haptic design facilitated the perioperative manipulation and placement of the IOL in a smaller capsular bag without impairing the other biomechanical properties of a single C-loop design. [less ▲]

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See detailIntraocular Lens Adsorbome: a Proteomic Study of Adsorbed Proteins onto Acrylic Materials and Its Implication in Secondary Cataract
Huang, Yi-Shiang ULg; Bertrand, Virginie ULg; Mazzucchelli, Gabriel ULg et al

Poster (2012, September 17)

The intraocular lens (IOL) is a polymer implant designed to replace the natural lens after cataract surgery. When the implant is introduced into the lens capsule, the polymer starts to interact with the ... [more ▼]

The intraocular lens (IOL) is a polymer implant designed to replace the natural lens after cataract surgery. When the implant is introduced into the lens capsule, the polymer starts to interact with the aqueous humour and the exchange of molecules between the solid and the liquid begins. The nature of exchange in water, ions, and biomolecules may result in several postoperative complications including glistening, calcification, and posterior capsular opacification. The posterior capsular opacification (PCO, also called “Secondary Cataract”) is raised from the over-growth of residual lens epithelial cells. The first step of the over-growth process of the cells is their adhesion to the deposited biomolecules, such as proteins involved in extra-cellular matrices. The purpose of this study is to identify the principal proteins adsorbed onto the acrylic polymers by mass spectrometry. The concept of adsorbome is to generate a list of adsorbed proteins to the hydrophilic and hydrophobic polymers, and then compare the difference to the original component of aqueous humour in order to see the affinity of individual protein to each material. Two kinds of hydrophilic and two kinds of hydrophobic acrylic polymers were tested for their adsorbomes by treating them with an aqueous humour analogue and the major adsorbed proteins were identified by mass spectrometry. Interestingly, the hydrophilic acrylic polymer shows a relative lower protein adsorption rate but shows a higher incidence of secondary cataract. This phenomenon implies the adsorbed proteins play a crucial role in progress of secondary cataract. [less ▲]

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See detailBioactive Intraocular Lens - A New Concept to Control Secondary Cataract
Huang, Yi-Shiang ULg; Alexandre, Michaël ULg; Bozukova, Dimitriya et al

Poster (2012, August 29)

A cataract is pathology opacity of the lens, causing impairment of vision or even blindness. Today, a surgery is still the only available treatment. The intraocular lens (IOL) is a polymer implant ... [more ▼]

A cataract is pathology opacity of the lens, causing impairment of vision or even blindness. Today, a surgery is still the only available treatment. The intraocular lens (IOL) is a polymer implant designed to replace the natural lens in the cataract surgery. The materials for IOL require excellent optical properties for light transmission, mechanical properties for folding injection during surgery, and biological properties for preventing body rejection. The biocompatibility - or more specified, bio-inert - seems to be the prerequisite in selecting the materials. [1] However, the bioinert materials could not satisfy the unmet need in the secondary cataract control. Posterior capsular opacification (PCO, or Secondary Cataract), characterized by a thick and cloudy layer of lens epithelial cells (LECs), is the most common postoperative complication. In 1997, a “Sandwich Theory” model was proposed to elucidate the developmental process of PCO. [2] In this model, the residual LECs between the lens capsular bag and the IOL undergo proliferation, migration, as well as transdifferentiation and finally induce PCO if the affinity to the IOL material is low. In our research, a bioactive molecule is introduced to the conventional acrylic hydrophilic polymer pHEMA(Poly(2-hydroxyethyl methacrylate)) by covalent conjugation. The RGD peptide sequence, being well-known for its tissue integration ability, is designed to stimulate the biointegration between the LECs and the IOL. [3]. Our data have shown the peptide grafted biomaterial not only exhibits similar optical and mechanical properties, but also reveals enhanced biological properties in cell adhesion and cell morphology maintenance. By means of surface functionalization of IOL to stabilize and restore LECs, the secondary cataract could be controlled in a regenerative medicine way. References [1] Dimitriya Bozukova (2010) Materials Science and Engineering R, 69: 63-83. [2] Reijo Linnola (1997) J Cataract Refract Surg., 10: 1539–42. [3] Ruoslahti E (1986) Cell, 44(4): 517-8. [less ▲]

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See detailComposite comprising nanoparticles and method of making nanoparticles
Jérôme, Christine ULg; Bozukova, Dimitriya

Patent (2012)

The invention relates to a method for the production of nanoparticles comprising a conjugated polymer. The method comprises (i) providing a reaction mixture with a monomer, a photosensitising agent and a ... [more ▼]

The invention relates to a method for the production of nanoparticles comprising a conjugated polymer. The method comprises (i) providing a reaction mixture with a monomer, a photosensitising agent and a solvent, and (ii) exposing the reaction mixture to photo-irradiation to form nanoparticles of a conjugated polymer wherein the photosensitising agent is immiscible with the solvent. [less ▲]

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See detailComposite comprising nanoparticles and method of making nanoparticles
Bozukova, Dimitriya; Jérôme, Christine ULg

Patent (2012)

A method for making nanoparticles of a conjugated polymer, the method having the following steps (i) providing a reaction mixture with a monomer, a photosensitising agent and a solvent, and (ii) exposing ... [more ▼]

A method for making nanoparticles of a conjugated polymer, the method having the following steps (i) providing a reaction mixture with a monomer, a photosensitising agent and a solvent, and (ii) exposing the reaction mixture to photo irradiation to form nanoparticles of a conjugated polymer. The composite compring the nanoparticles may be used in an intraocular lens implant. [less ▲]

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See detailSurface and bio-adhesion properties of new hydrophobic and current materials for artificial intraocular lens
Bertrand, Virginie ULg; Svaldo Lanero, Tiziana ULg; Duwez, Anne-Sophie ULg et al

Poster (2012)

A high bio-adhesion appears to be one of the key factor for posterior capsular opacification (PCO) prevention. Indeed, the proteins adsorption and the lens epithelial cells (LEC) adhesion both contribute ... [more ▼]

A high bio-adhesion appears to be one of the key factor for posterior capsular opacification (PCO) prevention. Indeed, the proteins adsorption and the lens epithelial cells (LEC) adhesion both contribute to PCO development. We present in this work the comparison of a new glistening free hydrophobic material (GF® from Physiol) with benchmark hydrophobic and hydrophilic materials regarding their chemicophysical properties and their respective ability to interact with lens epithelial cells and proteins. For this purpose, we determined the hydrophobicity by contact angle measurement (assessed by water drop and air bubble methods), the surface adhesiveness by atomic force microscopy (AFM), the proteins adsorption by fluorescent measurement and the LEC adhesion by the determination of cell density. The new hydrophobic material presents comparable hydrophobicity, proteins adsorption and LEC adhesion to current commercial hydrophobic material. Its adhesiveness, measured with the AFM, is intermediate between hydrophilic and hydrophobic materials. In conclusion, the bio-adhesion properties of this new glistening free hydrophobic IOL material are similar to generic hydrophobic acrylic materials and therefore should to the same extent prevent PCO. [less ▲]

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See detailAssessment of new-generation glistening-free hydrophobic acrylic intraocular lens material
Pagnoulle, Christophe; Bozukova, Dimitriya; Gobin, Laure et al

in Journal of Cataract & Refractive Surgery (2012), 38

To determine the hydrophobic, antiglistening, and bioadhesiveness properties of a new polymer, GF rawmaterial, and to determine the suitability of thismaterial for use in intraocular lenses (IOLs).

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See detailHydrogel nanocomposites: a potential UV/blue light filtering material for ophthalmic lenses
Bozukova, Dimitriya; Pagnoulle, Christophe; De Pauw, Marie-Claire ULg et al

in Journal of Biomaterials Science. Polymer Edition (2011), 22

Poly(2-hydroxyethyl methacrylate-co-methyl methacrylate) (poly(HEMA-co-MMA)) and ZnS hydrogel nanocomposites were prepared and characterized. The chemical composition of the inorganic nanoparticles was ... [more ▼]

Poly(2-hydroxyethyl methacrylate-co-methyl methacrylate) (poly(HEMA-co-MMA)) and ZnS hydrogel nanocomposites were prepared and characterized. The chemical composition of the inorganic nanoparticles was confirmed by X-ray diffraction, and the homogeneity of their distribution within the hydrogel was assessed by transmission electron microscopy. The influence of the content of ZnS nanoparticles on the optical performances of the nanocomposites was investigated by UV-Vis spectroscopy. The ability of the hydrogel nanocomposites to filter the hazardous UV light and part of the blue light was reported, which makes them valuable candidates for ophthalmic lens application. In contrast to the optical properties, the thermo-mechanical properties of neat poly(HEMA-co-MMA) hydrogels were found to be largely independent of filling by ZnS nanoparticles ( 2 mg/ml co-monomer mixture). Finally, in vitro cell adhesion test with lens epithelial cells (LECs), extracted from porcine lens crystalline capsule, showed that ZnS had no deleterious effect on the biocompatibility of neat hydrogels, at least at low content. [less ▲]

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See detailFlourinated material
Bozukova, Dimitriya; Jérôme, Christine ULg; Pagnoulle, Christophe

Patent (2010)

This invention relates to a method for modifying the surface of a material for use with a biological sample or tissue comprising the steps of providing a material having a surface which comprises reactive ... [more ▼]

This invention relates to a method for modifying the surface of a material for use with a biological sample or tissue comprising the steps of providing a material having a surface which comprises reactive-functional groups; providing fluorinated molecules having reactive-functional groups complimentary to those on the material surface; using wet chemistry to attach the fluorinated molecules to the surface of the material by reacting the reactive-functional groups of the implant with the complementary reactive-functional groups of the fluorinated molecules. The invention also relates to materials modified by said method and to implants made using said materials. [less ▲]

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See detailPolymers in modern ophthalmic implants—Historical background and recent advances
Bozukova, Dimitriya; Pagnoulle, Christophe; Jérôme, Robert ULg et al

in Materials Science & Engineering : R (2010), 69(6), 63-83

Cataract surgery is the most frequently performed surgical intervention, pursued by replacement of the opacified natural lens by a polymeric intraocular lens (IOL). This paper, based on an exhaustive ... [more ▼]

Cataract surgery is the most frequently performed surgical intervention, pursued by replacement of the opacified natural lens by a polymeric intraocular lens (IOL). This paper, based on an exhaustive number of scientific references, provides a brief simplified discussion of the surgical advances for cataract treatment, and is mainly focused on the process of IOL discovery, engineering and development. The performances of the polymer materials, used for the IOLs fabrication are discussed in a comparative way, and their properties, such as handling during surgery, biocompatibility, rate of some post-surgical complications (e.g. Posterior Capsular Opacification) and optical properties, are considered. Special attention is paid on the basic scientific approaches for engineering and modification of the IOL surface and bulk properties. Polymer coating techniques like ‘grafting onto’ and ‘grafting from’ are discussed and exemplified by a variety of coating compositions and performances. In another part of the review, modification techniques concerning optimization of the bulk properties of the polymer lens are also discussed and synthetic approaches such as copolymerization and nanocomposite formation are considered. A perspective aspect of this discussion concerns improvement of the performance of the main polymer by the incorporation of in situ formed or preformed nanoparticles. The most attractive perspectives concerning the improvement of the IOL properties by chemical modification approaches are described. [less ▲]

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See detailAntibacterial activity of poly(vinyl alcohol)-b-poly(acrylonitrile) based micelles loaded with silver nanoparticles
Bryaskova, Rayna; Pencheva, Daniela; Kyulavska, Mariya et al

in Journal of Colloid & Interface Science (2010), 344(2), 424-448

A new amphiphilic poly(vinyl alcohol)-b-poly(acrylonitrile) (PVOH-b-PAN) copolymer obtained by selective hydrolysis of well-defined poly(vinyl acetate)-b-poly(acrylonitrile) copolymer synthesized by ... [more ▼]

A new amphiphilic poly(vinyl alcohol)-b-poly(acrylonitrile) (PVOH-b-PAN) copolymer obtained by selective hydrolysis of well-defined poly(vinyl acetate)-b-poly(acrylonitrile) copolymer synthesized by cobalt mediated radical polymerization was used for the preparation of PVOH-b-PAN based micelles with embedded silver nanoparticles. The successful formation of silver loaded micelles has been confirmed by UV–vis, DLS and TEM analysis and their antibacterial activity against Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), Pseudomonas aeruginosa (P. aeruginosa) and spore solution of Bacillus subtilis (B. subtilis) has been studied. PVOH-b-PAN based micelles with embedded silver nanoparticles showed a strong bactericidal effect against E. coli, S. aureus and P. aeruginosa and the minimum bactericidal concentration for each system (MBC) has been determined. [less ▲]

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See detailPlasma surface fluorination of hydrogel materials-coating stability and in vitro biocompatibility testing
Bozukova, Dimitriya; Pagnoulle, Christophe; Gillet, Marie-Claire ULg et al

in Soft Materials (2010), 8(2), 164-182

Plasma enhanced chemical vapor deposition has been tested for the formation of hydrophobic perfluorinated coating on the surface of hydrophilic poly(2-hydroxyethyl methacrylate-co-methyl methacrylate ... [more ▼]

Plasma enhanced chemical vapor deposition has been tested for the formation of hydrophobic perfluorinated coating on the surface of hydrophilic poly(2-hydroxyethyl methacrylate-co-methyl methacrylate) [poly(HEMA-co-MMA)] substrates, used for the fabrication of intraocular lenses (IOLs). The properties of the dry and hydrated surface modified by two plasma techniques, Radio-frequency (RF) and Microwave (MW), were investigated in parallel by contact angle measurements in the dry and hydrated state, X-ray photoelectron spectroscopy, and atomic force microscopy. The coating stability and hydrophobicity were challenged by swelling and sterilizing the samples in water. Investigation of the optical performances of the modified samples was performed by ultraviolet spectroscopy and diopter measurements. Since materials with biomedical application are considered, the performances of their surface in contact with lens epithelial cells were tested at in vitro conditions, and repulsion was not found to be enhanced upon modification. Generally, the results showed poor stability of the coating and bring in question its covalent grafting to the surface. [less ▲]

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See detailToward protein and cell repellent properties by surface modification of intraocular lenses
Bozukova, Dimitriya; Pagnoulle, Christophe; De Pauw, Marie-Claire ULg et al

Poster (2008, June 23)

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See detailSurface coating of hydrogel intraocular lenses toward resistance to posterior capsular opacification
Bozukova, Dimitriya; Pagnoulle, Christophe; Gillet, Marie-Claire ULg et al

Conference (2008, May 23)

Detailed reference viewed: 46 (11 ULg)