References of "Bouché, Frédéric"
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See detailFlowering Goes Underground
Bouché, Frédéric ULg; Lobet, Guillaume ULg; D'Aloia, Maria ULg et al

Conference (2014, May 15)

Flowering is a crucial step in plant life cycle and is therefore tightly controlled by both environmental and endogenous cues. The involvement of the aerial organs of the plant in the molecular mechanisms ... [more ▼]

Flowering is a crucial step in plant life cycle and is therefore tightly controlled by both environmental and endogenous cues. The involvement of the aerial organs of the plant in the molecular mechanisms controlling floral transition has been extensively documented while the participation of the roots remains poorly investigated. However, the induction of flowering by photoperiod involves systemic signals that move in the phloem from leaves to sinks, and hence presumably reach the roots. We therefore performed a transcriptomic analysis of the roots during the induction of flowering in Arabidopsis thaliana and indeed identified a large number of differentially expressed genes. A reverse genetic approach further confirmed the pleiotropic effects of flowering time genes on root architecture. [less ▲]

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See detailThe hidden half of flowering
Bouché, Frédéric ULg; Mistiaen, Kevin ULg; D'Aloia, Maria ULg et al

Poster (2013, June)

Flowering is one of the most important developmental steps in plant life cycle and is therefore tightly controlled by environmental cues. The involvement of the aerial part of the plant in the molecular ... [more ▼]

Flowering is one of the most important developmental steps in plant life cycle and is therefore tightly controlled by environmental cues. The involvement of the aerial part of the plant in the molecular mechanisms leading to floral transition is well documented while participation of the roots received less attention. Nevertheless, the induction of flowering by photoperiod is known to involve systemic signals that move in phloem sap towards sinks, throughout the plants, including the roots. Transcriptomic analysis of roots tissues during the floral induction of flowering by a single long day of in Arabidopsis thaliana by a single long day allowed us to identify a large number of differentially expressed genes. How mutations We subsequently selected in some candidate genes affect plant development - including root architecture and flowering time - is being to analyze their flowering timefurther analyzed. Further analysis of those genes will permit us to unravel their role in the flowering induction process. [less ▲]

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See detailMolecular analysis of root medium impact on Arabidopsis thaliana development
Bouché, Frédéric ULg; André, Julie; Tocquin, Pierre ULg et al

Poster (2013, April 18)

Hydroponics and soil are the most common media used for plant growth. Hydroponics has the main advantage of providing easy access to the root system and is therefore commonly used for gene expression ... [more ▼]

Hydroponics and soil are the most common media used for plant growth. Hydroponics has the main advantage of providing easy access to the root system and is therefore commonly used for gene expression analyses in molecular studies of the model plant Arabidopsis thaliana. However, the impact of root substrate on plant growth remains poorly documented. Here we show that hydroponics accelerates both shoot growth and developmental phases as compared with culture on soil. In order to identify molecular changes in the roots that could account for these medium effects, a transcriptomic comparison was performed by microarray analysis. This experiment revealed that more than 20% of the genes were differentially expressed in hydroponics vs soil. Among them, the flowering time gene FLOWERING LOCUS C and two clades of microRNA targeted genes. To further assess the role of these genes in roots, artificial microRNAs were designed for root specific expression in transgenic Arabidopsis plants. [less ▲]

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See detailImplication of microRNAs in the response of Brachypodium distachyon (L.) Beauv. root system architecture to rhizobacterial volatiles
Baudson, Caroline ULg; Bouché, Frédéric ULg; Saunier de Cazenave, Magdalena ULg et al

Scientific conference (2013, February)

The metabolic roles of volatile organic compounds (VOCs) emitted by plant growth-promoting rhizobacteria (PGPR) and the identity of the molecules responsible for the growth promotion are still poorly ... [more ▼]

The metabolic roles of volatile organic compounds (VOCs) emitted by plant growth-promoting rhizobacteria (PGPR) and the identity of the molecules responsible for the growth promotion are still poorly documented. As well, the implication of microRNAs in root development is a recent discovery that deserves to be explored. In this study, the implication of microRNAs in the response of Brachypodium distachyon (L.) Beauv. Bd21 root architecture to rhizobacterial VOCs was investigated. Nineteen PGPR strains were screened to select those showing the strongest phenotypic effects. The strain Bacillus subtilis AP305-GB03 induced the most important promotion of biomass production and root development. Total RNA extraction and RT-qPCR analysis of microRNAs were performed on Bd21 root samples. The expression of miR160 a-d, miR164 f, miR167 c-d, miR397 b and miR399 a-b was measured in roots every 2 days during the first 10 days of Bd21 development, in the presence or absence of the bacterial VOCs. Differences in the expression profile of miR164 f and miR167 c-d were observed in the roots exposed to GB03 VOCs, as compared to the control. These differences could be correlated to the root system architecture modifications observed after 10 days of growth with GB03. miR397 b and miR399 a-b also showed differences in the expression profile of roots exposed to the bacterial VOCs. These microRNAs have been respectively involved in cold stress tolerance and in the response to phosphate starvation. [less ▲]

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See detailA root chicory MADS-box sequence and the Arabidopsis flowering repressor FLC share common features that suggest conserved function in vernalization and devernalization responses
Périlleux, Claire ULg; Pieltain, Alexandra; Jacquemin, Guillaume et al

in Plant Journal (The) (2013), 75

Root chicory (Cichorium intybus var. sativum) is a biennial crop, but is harvested for root inulin at the end of the first growing season before flowering. However, cold temperatures might vernalize seeds ... [more ▼]

Root chicory (Cichorium intybus var. sativum) is a biennial crop, but is harvested for root inulin at the end of the first growing season before flowering. However, cold temperatures might vernalize seeds or plantlets, leading to incidental early flowering and hence understanding the molecular basis of vernalization is important. A MADS-box sequence was isolated by RT-PCR and named FLC-LIKE1 (CiFL1) because of its phylogenetic positioning within the same clade as the floral repressor Arabidopsis FLOWERING LOCUS C (AtFLC). Moreover, overexpression of CiFL1 in Arabidopsis caused late flowering and prevented up-regulation of the AtFLC target FLOWERING LOCUS T gene by photoperiod, suggesting functional conservation between root chicory and Arabidopsis. Like AtFLC in Arabidopsis, CiFL1 was repressed during vernalization of seeds or plantlets of chicory, but repression of CiFL1 was unstable whether the post-vernalization temperature was favorable to flowering or whether it devernalized the plants. Instability of CiFL1 repression might be linked to bienniality of root chicory versus the annual life cycle of Arabidopsis. However, reactivation of AtFLC was also observed in Arabidopsis when a high temperature treatment was given straight after seed vernalization, erasing the promotive effect of cold on flowering. Cold-induced downregulation of a MADS-box floral repressor and its reactivation by high temperature thus appear as conserved features of the vernalization and devernalization responses in distant species.This article is protected by copyright. All rights reserved. [less ▲]

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See detailRooting the flowering process
D'Aloia, Maria ULg; Bouché, Frédéric ULg; Tamseddak, Karim et al

Poster (2012, May)

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See detailA cytokinin route to flowering in Arabidopsis
Bouché, Frédéric ULg; André, Julie ULg; D'Aloia, Maria ULg et al

Poster (2011, June)

Cytokinins (CKs) are involved in many physiological processes. We observed that the application of N6-benzylaminopurine (BAP) to the roots of hydroponically grown plants of Arabidopsis thaliana promotes ... [more ▼]

Cytokinins (CKs) are involved in many physiological processes. We observed that the application of N6-benzylaminopurine (BAP) to the roots of hydroponically grown plants of Arabidopsis thaliana promotes flowering in non-inductive short days. The response to BAP treatment does no require FLOWERING LOCUS T (FT), but activates its paralogue TWIN SISTER OF FT (TSF), as well as FD and SUPPRESSOR OF OVEREXPRESSION OF CO1 (SOC1) (D'Aloia et al., 2011). We present here complementary data obtained with transgenic plants overexpressing a catalytic CK OXIDASE/DEHYDROGENASE (CKX) in the roots. The high efficiency of BAP in promoting flowering in our experimental system contrasts with the variability that emerges from studies gathered in literature. Many factors, either experimental or inherent to plant material, might explain these discrepancies and we are interested in identifying endogenous regulators that might provide a mechanistic explanation. We are therefore investigating whether the endogenous pathways underlying plant developmental phase changes might regulate the relative contribution of CKs to flowering. [less ▲]

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See detailCytokinin promotes flowering of Arabidopsis via transcriptional activation of the FT paralogue TSF
D'Aloia, Maria ULg; Bonhomme, Delphine ULg; Bouché, Frédéric ULg et al

in Plant Journal (The) (2011), 65

Cytokinins are involved in many aspects of plant growth and development and physiological evidence also indicates that they have a role in floral transition. In order to integrate these phytohormones into ... [more ▼]

Cytokinins are involved in many aspects of plant growth and development and physiological evidence also indicates that they have a role in floral transition. In order to integrate these phytohormones into the current knowledge of genetically defined molecular pathways to flowering, we performed exogenous treatments of adult wild-type and mutant Arabidopsis plants and analysed the expression of candidate genes. We used a hydroponic system that enables synchronous growth and flowering of Arabidopsis and allows precise application of chemicals to the roots for defined periods of time. We show that application of N6-benzylaminopurine (BAP) promotes flowering of plants grown in non-inductive short days. The response to cytokinin treatment does not require FLOWERING LOCUS T (FT) but activates its paralogue TWIN SISTER OF FT (TSF), as well as FD, which encodes a partner protein of TSF, and the downstream gene SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (SOC1). Treatment of selected mutants confirmed that TSF and SOC1 are necessary for the flowering response to BAP while the activation cascade might partially act independently of FD. These experiments provide a mechanistic basis for the role of cytokinins in flowering and demonstrate that the redundant genes FT and TSF are differently regulated by distinct floral inducing signals. [less ▲]

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