References of "Blaise, Yannick"
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See detailA mucin-enriched fermentation model to assess prebiotic potential of new indigestible carbohydrates
Boudry, Christelle ULg; Tran, Thi Hanh Tham ULg; Blaise, Yannick ULg et al

in 64th Annual Meeting of the European Federation of Animal Science (2013, August 29)

Screening the prebiotic potential of novel indigestible carbohydrates (ICH) is a challenge for feed and food industry and in vitro models are increasingly used for such purposes. Recently extracellular ... [more ▼]

Screening the prebiotic potential of novel indigestible carbohydrates (ICH) is a challenge for feed and food industry and in vitro models are increasingly used for such purposes. Recently extracellular binding proteins responsible for the adherence to intestinal mucus were described for several Lactobacillus species. As this genus is known for its beneficial effect on gut health, we enriched the in vitro gas fermentation model with mucin in order to evaluate the prebiotic potential of 5 ICH. Mucin-coated microcosms (MCM) were prepared as described by Van den Abbeele et al. (2012, Microbial Biotechnology, 5, 106-115) and introduced in the fermentation bottles with an inoculum prepared from fresh faeces of 3 sows mixed with a nutritive buffer solution. Fermentation was performed at 39°C, using 200 mg of substrate, 30 ml of inoculum and 6 MCM, yielding approx. 20 mg mucin each, in 140 ml glass bottles. A first study was performed with inulin and cellulose as substrates, with and without mucus in the bottles. A second study was performed with 5 substrates (inulin, IMO, beet pulp POS, cellobiose and gluconate) in presence of mucus. After 8 and 72h, SCFA and the microflora of fermentation broth was analysed as well as the microflora on the MCM. The comparison of the microflora evolution with and without mucus showed a better development of the Lactobacillus in the fermentation broth, mainly in presence of inulin. The development of the Lactobacillus genus allowed the classification of the 5 substrates tested in the second study (Inulin > IMO > Gluconate > Cellobiose > POS)(P < 0.05) which was not possible without mucus (P > 0.05). Inulin and IMO showed also the highest development of Bifidobacteria (P < 0.05) and the highest levels of butyrate production (P < 0.05) compared to the three other substrates, indicating a high prebiotic potential. [less ▲]

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See detailIn vitro determination of prebiotic potential of sugar beet pulp extracted candidates and influence of production method
François, Emmanuelle ULg; Goffin, Dorothée ULg; combo, Agnan Marie-Michelle et al

Poster (2012, June 12)

Various prebiotic hydrolysates were produced starting from sugar beet pulp (SBP) using alternative solution to traditional acidic hydolysis. SBP pectin was first extracted by acid extraction and ethanol ... [more ▼]

Various prebiotic hydrolysates were produced starting from sugar beet pulp (SBP) using alternative solution to traditional acidic hydolysis. SBP pectin was first extracted by acid extraction and ethanol precipitation. Then two technics are used to produced pectic oligosaccharides (POS) : enzymatic hydrolysis (EnzPOS : Rapidase Smart (DSM) ; 50°C ; pH 5) and microwaves-assisted hydrolysis (MW1POS : 104°C/10min/pH 7; MW2POS : 110°C/15min/pH 7). Structural characterization of fractions gave rise to different structural profiles between the four products. Moreover, fermentation parameters obtained in-vitro (A, B, Rmax and Tmax) were also characteristic of the fractions and in favor of a tight relationship between POS structure and POS function. Finally, to avoid solvent use, POS production could be envisaged directly on SBP. Avoiding the step of acidic extraction will allow to meet the green chemistry principles. [less ▲]

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See detailImpact of mixing imperfections on yeast bioreactor performances: Scale-down reactor concept and related experimental tools
Delvigne, Frank ULg; Blaise, Yannick ULg; Destain, Jacqueline ULg et al

in Cerevisia and Biotechnology (2012), 37

A method combining environmental data extracted from the dissolved oxygen profile of a fed-batch bioreactor and a dynamic discrete Markov chain model has been presented in order to give more insight about ... [more ▼]

A method combining environmental data extracted from the dissolved oxygen profile of a fed-batch bioreactor and a dynamic discrete Markov chain model has been presented in order to give more insight about the glucose and dissolved oxygen fluctuations experienced by the microorganisms during cultivation in heterogeneous bioreactor. The fed-batch cultivation of Saccharomyces cerevisiae has been performed in a well-mixed and a partitioned scale-down reactor (SDR). The analysis of the environmental sequences has shown extended time lengths for the glucose availability and depletion sequences in the case of the SDR under a DO-controlled fed-batch culture. The Markov chain model developed in this work is able to capture the stochastic environmental events, i.e. in our case the environmental states experienced by the microorganisms crossing the tubular part of the SDR. The simulation results show clearly an extension of the starvation periods in the case of the culture performed in the SDR. The simulations have been performed at the single cells level allowing future improvements of our model and notably in the context of the population segregation phenomena occurring in fed-batch cultures. As a perspective, flow cytometry has been presented as a high-throughput analytical tool for the investigation of yeast physiology at the single cell level and in process-related conditions. [less ▲]

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