References of "Biver, Sophie"
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See detailIdentification and Characterization of a Halotolerant, Cold-Active Marine Endo-β-1,4-Glucanase by Using Functional Metagenomics of Seaweed-Associated Microbiota
Martin, Marjolaine ULg; Biver, Sophie ULg; Steels, Sébastien ULg et al

in Applied and Environmental Microbiology (2014), 80(16), 4958-4967

A metagenomic library was constructed from microorganisms associated with the brown alga Ascophyllum nodosum. Functional screening of this library revealed 13 novel putative esterase loci and two ... [more ▼]

A metagenomic library was constructed from microorganisms associated with the brown alga Ascophyllum nodosum. Functional screening of this library revealed 13 novel putative esterase loci and two glycoside hydrolase loci. Sequence and gene cluster analysis showed the wide diversity of the identified enzymes and gave an idea of the microbial populations present during the sample collection period. Lastly, an endo-β-1,4-glucanase having less than 50% identity to sequences of known cellulases was purified and partially characterized, showing activity at low temperature and after prolonged incubation in concentrated salt solutions. [less ▲]

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See detailBacillus subtilis as a tool for screening soil metagenomic libraries for antimicrobial activities
Biver, Sophie ULg; Steels, Sébastien ULg; Portetelle, Daniel ULg et al

in Journal of Microbiology and Biotechnology (2013), 23(6), 850-855

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See detailCharacterization of new bacterial glycoside hydrolases isolated from agricultural soils using a functional metagenomic approach
Biver, Sophie ULg; Dubois, Benjamin; Stroobants, Aurore ULg et al

Poster (2013, June 10)

Microorganisms play key roles in soil ecosystem functioning, notably through their ability to degrade plant cell wall polymers. For this, bacteria and fungi produce various enzymes such as cellulases ... [more ▼]

Microorganisms play key roles in soil ecosystem functioning, notably through their ability to degrade plant cell wall polymers. For this, bacteria and fungi produce various enzymes such as cellulases, xylanases, glucosidases, esterases or laccases. Finding new enzymes hydrolyzing cellulose, hemicellulose or lignin is not only interesting for a better understanding of the roles of the soil microflora still largely unknown but these enzymes are also useful for various biotechnological applications such as the production of renewable energy from lignocellulosic material. So here, we used a functional metagenomic approach to isolate new bacterial β-glucosidases, which were then biochemically characterized. The new enzymes were identified by functional analysis of agricultural-soil metagenomic libraries hosted in Escherichia coli and screened on medium containing esculin. After sequence analysis and preliminary estimation of the activity of the new β-glucosidases using p-nitrophenol derivatives on intact bacterial cells, the coding sequences of three of them were cloned into a bacterial expression vector so as to overproduce and purify them by affinity chromatography. The chosen enzymes show only 52-64% sequence identity to known family 3 (GH3) or 1 (GH1) glycoside hydrolases of different phyla (Actinobacteria, Acidobacteria and Proteobacteria). Analysis of the E. coli cells expressing each of them revealed that both GH1 proteins (ASEsc9 and ASEsc10) are thermophilic enzymes more active at mildly acidic to neutral pH while the GH3 enzyme (ASEsc6) is an alkaline, mesophilic, β-glucosidase also displaying xylosidase activity. Their coding sequences have been cloned in fusion with a carboxy-terminal His-tag and placed under the control of the IPTG-inducible promoter of the pET-30b vector. The proteins will be overproduced and purified for further characterization. [less ▲]

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See detailBiomass hydrolyzing enzymes identified by functional screening of a metagenomic library from algal biofilms.
Martin, Marjolaine ULg; Biver, Sophie ULg; Barbeyron, Tristan et al

Poster (2013, June)

Biomass hydrolyzing enzymes are increasingly searched for the production of biofuels and renewable chemical compounds using biomass. Microorganisms living on algaes are an interesting reservoir of biomass ... [more ▼]

Biomass hydrolyzing enzymes are increasingly searched for the production of biofuels and renewable chemical compounds using biomass. Microorganisms living on algaes are an interesting reservoir of biomass hydrolyzing enzymes, as they are in constant interaction with algal biomass. Therefore, we are interested in looking for some of those enzymes synthesized by the microflora living on the surface of the brown algae Ascophyllum nodosum. Algae samples were collected in the winter 2012 and a microbial DNA extraction method was developed. The whole extracted microbial genomes of the microorganisms living on the algae were restricted, inserted in a cloning vector and ligated products were used for transformation of cultivable Escherichia coli host cells. This metagenomic library was then screened for diverse enzymatic activities (lipolytic enzymes, cellulases, beta-glucosidases, alpha-amylases, arabinanases, xylanases and proteases) on agar plates with specific substrates. Five putative lipolytic enzymes, one cellulase and one beta-glucosidase were identified. Sequence analysis revealed low (<50%) sequence identities with known enzymes sequences, meaning new enzymes from unknown genomes have been discovered. To our knowledge this is the first functional screening that was realized with a metagenomic library from algal biofilms and this is the first cellulase identified by marine metagenomics. A second library has been constructed from algae sample from summer 2012 and is currently being screened. New enzymatic tests are being developed for the identification of enzymes degrading specific algal polysaccharides like agarases, carrageenases, alginate lyases, laminarinases,… Those very specific enzymes aren’t well known yet, and our metagenomic approach will probably help us to identify new families and structures of those algal biomass hydrolyzing enzymes. [less ▲]

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See detailMicroorganisms living on algae : An interesting reservoir of enzymes hydrolyzing algal biomass
Martin, Marjolaine ULg; Biver, Sophie ULg; Barbeyron, Tristan et al

Poster (2013, April 18)

Algal polysaccharides are increasingly used in food industry for their gelling properties and in pharmacology for their therapeutic properties. Furthermore, increasingly interest is taken on algae for ... [more ▼]

Algal polysaccharides are increasingly used in food industry for their gelling properties and in pharmacology for their therapeutic properties. Furthermore, increasingly interest is taken on algae for their use in the production of biofuels and bioenergies. To purify algal polysaccharides and degrade algal biomass, specific microbial enzymes are needed. Microorganisms living on algae are an interesting source of those enzymes, as they are in constant interaction with algal biomass. The aim of our study is to identify new enzymes degrading algae, produced by microorganisms living on the surface of algae. Therefore we developed a method for microbial DNA extraction from biofilms living on brown algae (Ascophyllum nodosum). Microbial DNA was extracted, restricted and inserted in cultivable host cells of Echerichia coli, for the construction of our metagenomic DNA library. This metagenomic library was first screened, on solid media with specific substrates, for enzymes generally used in the degradation of biomass (lipases, cellulases, proteases, beta-glucosidases, alpha-amylases, arabinanases and xylanases). Five lipolytic enzymes, one beta-glucosidase and one cellulase were identified. Those enzymes show very low percentages of sequences identities with known enzymes, meaning we identified new and unknown enzymes. Those enzymes and their activity are being characterized. Preliminary tests show interesting results, like a cellulase active at low temperature. Screening tests are now being developed to identify enzymes hydrolyzing algal polysaccharides like agarases, carrageenases, alginate lyases, laminarinases,… Those enzymes aren’t well known yet and we hope to identify new enzymes (families) with our rich DNA library by our approach. [less ▲]

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See detailCharacterization of three new carboxylic ester hydrolases isolated by functional screening of a forest-soil metagenomic library
Biver, Sophie ULg; Vandenbol, Micheline ULg

in Journal of Industrial Microbiology & Biotechnology (2013), 40(2), 191-200

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See detailIdentification of new microbial enzymes from forest and marine ecosystems by functional metagenomics
Martin, Marjolaine ULg; Biver, Sophie ULg; Barbeyron, Tristan et al

Poster (2012, August 21)

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See detailFunctional suppression of the yeast msb3- mutation
Biver, Sophie ULg; Vandenbol, Micheline ULg

Poster (2010, September)

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See detailA role for Rhesus factor Rhcg in renal ammonium excretion and male fertility
Biver, Sophie ULg; Belge, Hendrica; Bourgeois, Soline et al

in Nature (2008), 456(7220), 339-343

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See detailPhysiological role of the putative ammonium transporter RhCG in the mouse
Biver, Sophie ULg; Scohy, Sophie; Szpirer, Josiane et al

Conference (2006, May 05)

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See detailPhysiological role of the putative ammonium transporter RhCG in the mouse
Biver, Sophie ULg; Scohy, Sophie; Szpirer, Josiane et al

in Transfusion Clinique et Biologique (2006), 13(1-2), 167-168

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