References of "Bindelle, Jérôme"
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See detailHealth relevance of intestinal protein fermentation in young pigs
Pieper, Robert; Villodre Tudela, Carmen; Taciak, Marcin et al

in Animal Health Research Reviews (in press)

The physiological role of the gastrointestinal microbiota has become an important subject of nutrition research in pigs in the past years, and the importance of intestinal microbial activity in the ... [more ▼]

The physiological role of the gastrointestinal microbiota has become an important subject of nutrition research in pigs in the past years, and the importance of intestinal microbial activity in the etiology of disease is doubtless. This review summarizes the recent knowledge related to the microbial ecology of protein fermentation and the appearance of protein-derived metabolites along the pig intestine. The amount of fermentable protein depends on factors such as dietary protein concentration, protein digestibility due to secondary or tertiary structure, the interaction with dietary compounds or anti-nutritional factors, and the secretion of endogenous proteins into the gut lumen. High protein diets increase the luminal concentrations and epithelial exposure to putatively toxic metabolites and increase the risk for post-weaning diarrhea, but the mechanisms are not yet clarified. Although the use of fermentable carbohydrates to reduce harmful protein-derived metabolites in pigs is well-established, recent studies suggest that the inclusion of fermentable carbohydrates into diets with low protein digestibility or high dietary protein level may not ameliorate all negative effects with regard to epithelial response. Based on the current knowledge, the use of diets with low levels of high-quality protein may help to reduce the risk for intestinal disease in young pigs. [less ▲]

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See detailImpact of cooking and species on intestinal fermentation patterns of vegetables in a humanized in vitro model of the gastro-intestinal tract
Kalala Bolokango, Gaetan ULg; Kambashi Mutiaka, Bienvenu ULg; Njeumen Lemotio, Georges Patrick ULg et al

Poster (2016, June 21)

Obesity and associated pathologies have dramatic consequences on patients’lives as well as high societal costs. Because of the role of intestinal dysbiosis and microbiota make-up on the pathogenesis of ... [more ▼]

Obesity and associated pathologies have dramatic consequences on patients’lives as well as high societal costs. Because of the role of intestinal dysbiosis and microbiota make-up on the pathogenesis of obesity, several strategies such as eating prebiotics and dietary fibre supplements are being investigated to reshape the intestinal microbial communities of obese patients. Beyond supplement, dietary fibre is supplied through plant ingredients in the meals. In the framework of the multidisciplinary research project Food4Gut, the use of vegetables rich in specific targeted dietary fiber, namely fructans, is being scrutinized for its ability to induce positive changes in the intestinal ecophysiology. Because expected effect might differ according the content in dietary fibre and fructans, the soluble:insoluble ratio, as well as the cooking of the vegetables, the fermentation patterns of several vegetables are being investigated in an dual in vitro model combining enzymatic hydrolysis to an in vitro fermentation step using faecal inoculums from humans, to evaluate the performance of gut microbiota, modulation of metabolic functions. Six vegetables were sampled in triplicates (N=3) and steamed for 20 to 30 min.: Jerusalem artichoke, salsify, asparagus, pumpkin, fennel and swede. They were chosen because they display a variety of contents in fructans, soluble (SDF) and insoluble dietaryfibre (IDF). Steamed vegetable samplesand burgers from local fast food restaurants (negative control) were hydrolyzed in vitro why porcine pepsin and pancreatin to mimic digestion in the upper gut and indigested fiber residues were recover using a 6kDa dialysis membrane.Subsequently, in vitro fermentation is being run with independent fecal inoculums from obese and lean patients (N=4). Fermentation kinetics over 24h as well as short-chain fatty acid production and profiles will be compared according to the individual donor and the vegetable species and multivariate analysis will be used to explore the relationships between donor, vegetable species and composition and fermentation patterns. [less ▲]

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See detailTeneurs en fibres de légumes cultivés en Wallonie
Bindelle, Jérôme ULg

Conference given outside the academic context (2016)

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See detailEffects of protein source and cooking procedure on intestinal microbiota and on fermentation end-products in rats
POELAERT, Christine ULg; Despret, Xavier; Portetelle, Daniel ULg et al

Poster (2016, June)

Animal and plant proteins are major proteins sources in the human diet. After their enzymatic degradation in the upper gastro-intestinal tract, the undigested fraction of these proteins is available for ... [more ▼]

Animal and plant proteins are major proteins sources in the human diet. After their enzymatic degradation in the upper gastro-intestinal tract, the undigested fraction of these proteins is available for fermentation by the microbiota of the large intestine leading to the production of short-chain fatty acids (SCFA), branched-chain fatty acids (BCFA), ammonia, biogenic amines, sulphur metabolites, phenols and indoles. As some of these compounds have genotoxic and cytotoxic effects, protein fermentation is considered as detrimental to the host’s epithelial health. BCFA are usually used as a marker of intestinal protein fermentation. We studied in vivo the impact of proteins from animal and plant origin, raw or after a cooking procedure, on the composition of gut microbiota and on fermentation end-products. Weanling rats were used as models of the human gut microbiota. Eight experimental diets were formulated with beef meat (Longissimus dorsi), chicken meat (Pectoralis major), white pea beans (Phaseolus vulgaris), soybeans (Glycine max), used raw and cooked, as sole source of protein in the diet. One casein diet was used as control. All diets, formulated to contain 15% of raw protein, were given to seven rats for four weeks. After euthanasia, caecal contents were collected. Pyrosequencing analyses (Roche 454 GS Junior Genome Sequencer) were performed to study the microbial composition. SCFA and BCFA were measured using HPLC (Waters 2690). Microbial composition in the caecum is associated to the type of dietary protein and to the cooking procedure applied. The proportion of BCFA in the caecal content is mainly affected by the type of protein. So BCFA represent respectively 04-06% and 35-44% of total SCFA with diets based on plant and on animal proteins. In conclusion, both the type of protein and the cooking procedure could impact the gut microbiota in terms of composition and of fermentative capacity. [less ▲]

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See detailA review on the use of sensors to monitor cattle jaw movements and behavior when grazing
Andriamandroso, Andriamasinoro ULg; Bindelle, Jérôme ULg; Mercatoris, Benoît ULg et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment (2016), 20

Precision Livestock Farming (PLF) is spreading rapidly in intensive cattle farms. It is based on the monitoring of individuals using different kinds of sensors. Applied to grazing animals, PLF is mainly ... [more ▼]

Precision Livestock Farming (PLF) is spreading rapidly in intensive cattle farms. It is based on the monitoring of individuals using different kinds of sensors. Applied to grazing animals, PLF is mainly based on the recording of three parameters: the location, the posture and the movements of the animal. Until now, several techniques have been used to discriminate grazing and ruminating behaviors with accuracies over 90% on average, when compared to observations, providing valuable tools to improve the management of pasture and grazing animals. However, bites and jaw movements are still overlooked, even though they are of utmost importance to assess the animal grazing strategies for various pasture types and develop future techniques allowing better estimation of their intake. The goal of this review is to explore the possibility of monitoring the individual jaw movements and the differentiation of bites in grazing animals. For this purpose, (1) the mechanisms of forage intake in cattle are explained briefly in order to understand the movements performed by the cow, especially during grazing, (2) the various sensors that have been proposed to monitor jaw movements of ruminants such as mechanical sensors (pressure sensors), acoustic sensors (microphone) and electromyography sensors are compared and (3) finally the relationship between jaw movements, biting behavior and forage intake is discussed. The review clearly demonstrated the abilities of mechanical, acoustic and electromyography sensors to classify the difference types of jaw movements. However, it also indicated a wide range of accuracies and different observation windows required to reach these accuracies when compared to the observed movement. This classification purpose could lead to a better detection of more specific behavior, e.g. bite detection, and their exact location on pasture. [less ▲]

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See detailHow Bees Deter Elephants: Beehive Trials with Forest Elephants (Loxodonta africana cyclotis) in Gabon
Ngama, Steeve ULg; Korte, Lisa; Bindelle, Jérôme ULg et al

in PLoS ONE (2016), 11(5), 12

In Gabon, like elsewhere in Africa, crops are often sources of conflict between humans and wildlife. Wildlife damage to crops can drastically reduce income, amplifying poverty and creating a negative ... [more ▼]

In Gabon, like elsewhere in Africa, crops are often sources of conflict between humans and wildlife. Wildlife damage to crops can drastically reduce income, amplifying poverty and creating a negative perception of wild animal conservation among rural people. In this context, crop-raiding animals like elephants quickly become “problem animals”. To deter elephants from raiding crops beehives have been successfully employed in East Africa; however, this method has not yet been tested in Central Africa. We experimentally examined whether the presence of Apis mellifera adansonii, the African honey bee species present in Central Africa, deters forest elephants (Loxodonta Africana cyclotis) from feeding on fruit trees. We show for the first time that the effectiveness of beehives as deterrents of elephants is related to bee activity. Empty hives and those housing colonies of low bee activity do not deter elephants all the time; but beehives with high bee activity do. Although elephant disturbance of hives does not impede honey production, there is a tradeoff between deterrence and the quantity of honey produced. To best achieve the dual goals of deterring elephants and producing honey colonies must maintain an optimum activity level of 40 to 60 bee movements per minute. Thus, beehives colonized by Apis mellifera adansonii bees can be effective elephant deterrents, but people must actively manage hives to maintain bee colonies at the optimum activity level. [less ▲]

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See detailEffects of new potential prebiotics on Salmonella Thyphimurium in pigs
Tran, Thi Hanh Tham; Everaert, Nadia ULg; Boudry, Christelle et al

Conference (2016, April 15)

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See detailImpact of wheat bran supplementation to sows on their milk quality, their performances and their progeny’s
Leblois, Julie ULg; Bindelle, Jérôme ULg; Dehareng, Frédéric et al

Conference (2016, April 15)

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See detailEarly life programming of pigs' intestinal microbiota, intestinal functioning and hepatic metabolism by maternal wheat bran supplementation
Leblois, Julie ULg; Bindelle, Jérôme ULg; Dehareng, Frédéric et al

Poster (2016, February 05)

The gastrointestinal (GI) microbiota plays many roles on the host’s health, acting as a barrier against pathogens and influencing the development and maturation of the mucosa, important for host’s ... [more ▼]

The gastrointestinal (GI) microbiota plays many roles on the host’s health, acting as a barrier against pathogens and influencing the development and maturation of the mucosa, important for host’s immunity. Microbial colonization occurs pre- and postnatally, via maternal transfer i.e. by milk and by the contact with sows faeces. Hence, the early establishment of a beneficial gastrointestinal microbiota in piglets was investigated by supplementing the sows with wheat bran that we consider as a prebiotic (rich in non-starch polysaccharides). Sows were fed either a wheat bran-enriched diet (25% in gestation, 14% in lactation) either a control diet. Piglets were suckling during 4 weeks, receiving a standard creep feed containing no pre- or probiotic from the second week until weaning. The direct effect of wheat bran on the fecal microbial composition of the sow has been analyzed as well as the chemical composition and immunoglobulins content of the colostrum and milk. Sows and piglets growth performances have been recorded at different time points to verify that wheat bran doesn’t impair performances. At weaning, piglets have been euthanized and the impact of the maternal treatment was investigated at different levels: growth performances, ileal and colonic microbiota, intestinal physiology and immunological response and metabolism. A second animal experiment will be performed next year including a metabolic challenge by giving half of the piglets a high-energy diet post-weaning. [less ▲]

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See detailIn vitro evaluation of protein precipitation capacity of temperate browse species
Vandermeulen, Sophie ULg; Leblois, Julie ULg; Ramírez-Restrepo, Carlos Alberto et al

Poster (2016, February 05)

European agri-environmental policies are promoting the establishment of shrubs and trees on grasslands. The use of browse as fodder requires knowledge on their nutritive value since intensive production ... [more ▼]

European agri-environmental policies are promoting the establishment of shrubs and trees on grasslands. The use of browse as fodder requires knowledge on their nutritive value since intensive production systems are still relying on expensive and environment-costing protein sources. However, information on the influence of temperate condensed tannins (CT)-containing browse forage on rumen protein metabolism is elusive. The study aimed to assess the protein precipitation capacity (PPC) of 10 temperate browse species and establish the correlation between PPC values and plants CT content. PPC of foliage of 3 individuals per woody plants was measured using 2 model proteins: bovine serum albumin (BSA) and casein. The N content in protein solutions (4.6g/L; pH=6.8) was determined before and after adding each forage sample. Extractable CT concentration was quantified by spectrophotometry. The PPC varied across plant species (P<0.001). Corylus avellana had the highest ability to precipitate casein (52.4%). In contrast, the BSA precipitation (18.3%) of this plant was similar to Cornus sanguinea (12.7%), Quercus robur (12.1%) and Crataegus monogyna (11.0%). CT content ranged from 1.4 in Fraxinus excelsior to 82.7g/kg of depigmented sample in Corylus avellana (P<0.001) and was correlated to BSA (r=0.70; P<0.001) and casein PC (r=0.51; P<0.01). It was concluded that woody species could play a significant role in modifying protein metabolism, but further in vivo trials are required. [less ▲]

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See detailLINKING CATTLE GRAZING BEHAVIOR TO METHANE AND CARBON DIOXIDE DYNAMICS
Blaise, Yannick ULg; Lebeau, Frédéric ULg; Andriamandroso, Andriamasinoro ULg et al

in Communications in Agricultural and Applied Biological Sciences (2016, February), 81(1), 107-112

Various methods are presently used to measure methane (CH4) emissions of ruminants on pasture. Those measurements are essential to evaluate nutritional strategies to mitigate enteric CH4 emissions as well ... [more ▼]

Various methods are presently used to measure methane (CH4) emissions of ruminants on pasture. Those measurements are essential to evaluate nutritional strategies to mitigate enteric CH4 emissions as well as addressing the selection of low producing individuals. On pasture and in the barn, variations in CH4 emissions are observed depending on the time of the day. However, no studies have been made to link these diurnal fluctuations to behavioural phases, especially on pasture. The aim of this study was to understand the individual dynamics of CH4 production and their links to the grazing behaviour. For this purpose, a new tool was specifically developed. Five red-pied dry cows were equipped with infrared CH4 and carbon dioxide (CO2) sensors measuring concentrations in the exhaled air at 4 Hz. The animals were equipped with a heart rate belt (HR) and motion sensors to detect their feeding behaviours (grazing vs. rumination) for periods of 8 h/d. Wind speed (WS) was also monitor to verify interference with sampled gas concentrations. Results showed that using the CH4:CO2 ratio reduced the interference with WS that was observed on raw CH4 and CO2 concentration signals. CH4:CO2 ratio average over 5 min periods indicated that CH4 emissions were lower during grazing than rumination (P<0.01). The eructation frequency during grazing (0.48 eructation/min, P<0.01) was also lower than during rumination (0.65 eructation/min). HR was higher during grazing that rumination. Because HR is usually linked to metabolic CO2 production intensity, hence influencing the denominator of the CH4:CO2 ratio, further investigation should focus on the quantification of changes in fermentative and metabolic CO2 emissions along the day to estimate total CH4 production more accurately and the relationship between CH4 emissions patterns and post-feeding times. [less ▲]

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See detailOUTDOOR MEASUREMENT OF CATTLE METHANE EMISSIONS USING THE EDDY-COVARIANCE TECHNIQUE IN COMBINATION WITH GEOLOCALIZATION DEVICES
Dumortier, Pierre ULg; Andriamandroso, Andriamasinoro ULg; Aubinet, Marc ULg et al

Poster (2016, February)

Methane emissions account for 8% of the EU-15 GHG emissions and livestock generates approximately half of these emissions [1]. In order to improve emissions reporting and properly test mitigation options ... [more ▼]

Methane emissions account for 8% of the EU-15 GHG emissions and livestock generates approximately half of these emissions [1]. In order to improve emissions reporting and properly test mitigation options, techniques for measuring methane emissions from cattle must be developed and adapted to each management system. Among available micrometeorological methods, the use of eddy-covariance is still in its infancy [2] and its relevance and robustness for cattle flux estimation has still to be proved. On one hand, it is well adapted to seasonal grazing systems, is non-invasive, needs little animal handling and allows detection of daily emission patterns. On the other hand, it has the drawback of requiring cattle geo-localization and long periods of measurements (typically one month). In this study, we combined measured CH4 fluxes with a footprint model [3] and cattle positions (GPS devices) over several one-month campaigns at key periods in the grazing season in order to obtain CH4 emissions per cow at herd scale. Accelerometers were also added to the system for behaviour detection, opening the possibility of linking emissions to feeding behaviour. Measurements were performed and are still ongoing at the Dorinne Terrestrial Observatory in 2014/2015. The first campaign provided a mean emission per cow of 65±6 kg CH4.LSU-1.year-1. Cattle emission pattern was tightly linked with behaviour pattern, emissions being higher during and shortly after grazing (i.e. at dawn and dusk). Uncertainties linked to the method will be discussed and quantified (footprint model validity, geo-localization precision, eddy covariance corrections and filtering specificities linked to CH4 measurements). Compilation of data from multiple campaigns will allow quantification of the effects of forage quality, animal weight and lactating state on emissions per cow. [less ▲]

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See detailReview of shrubs and trees in intensive ruminant systems in temperate areas
Vandermeulen, Sophie ULg; Ramìrez-Restrepo, Carlos Alberto; Beckers, Yves ULg et al

Conference (2016, January)

Using shrubs and trees as forage for ruminants is common in extensive production systems in the tropics, as well as the Mediterranean region and mountain areas. In temperate Europe, the intensification of ... [more ▼]

Using shrubs and trees as forage for ruminants is common in extensive production systems in the tropics, as well as the Mediterranean region and mountain areas. In temperate Europe, the intensification of agriculture led to a decline in the numbers of woody perennials on farmlands. A review of the potential uses of shrubs and trees in temperate intensive systems shows that this concept is rather recent. Few studies have been investigating the potential outputs and limitations of shrubs and trees forage in production systems, while in Belgium and other European countries, agro-environmental policies are promoting the establishment of hedgerows and woody strips that provide shelter to animals against variable climate conditions. Furthermore, it has been found that ruminant species browse the plants, or alternatively, the forage is harvested and fed fresh or preserved as hay, silages or pellets. In both cases, consequences on feed intake control, woody plant survival, dry matter (DM) production and forage quality in terms of crude protein content reduction have been documented. In addition, depending on the plant species and the preservation method, bio-active plant metabolites such as condensed tannins (CT) are also present in the range of less than 1 to more than 100 g/kg foliage DM. Overall, CT may reduce ruminal N degradation, methanogenesis and nematode parasites infestation, while enhancing microbial-protein synthesis, feed use efficiency and systemic animal physiology. Planting shrubs and trees into the agricultural landscape (i.e. silvopastoral system) can further improve biodiversity and environmental services. Nevertheless, agronomic practices, farm management or environmental policy limitations may reduce the use of this fodder resource. Therefore, although silvopastoral systems seem promising in temperate ruminant systems, the current knowledge to their introduction and efficient management need to be cautiously considered. [less ▲]

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See detailHigh rate monitoring CH4 production dynamics and their link with behavioral phases in cattle
Blaise, Yannick ULg; Lebeau, Frédéric ULg; Andriamandroso, Andriamasinoro ULg et al

in EAAP – 67 th Annual Meeting, Belfast 2016 (2016)

Microbial fermentation in the rumen produces methane (CH4) which is a loss of energy for ruminants and also contributes to global warming. While the respiration chamber is the standard reference for CH4 ... [more ▼]

Microbial fermentation in the rumen produces methane (CH4) which is a loss of energy for ruminants and also contributes to global warming. While the respiration chamber is the standard reference for CH4 emissions quantification, daily CH4 production dynamics can be measured only by steps of 30 min and measurements on pasture are impossible. The alternative method using SF6 as tracer gas can be applied for grazing animals but provides average CH4 production values over at least several hours, making it impossible to measure short term dynamics of rumen CH4 production with changing animal behavior along the day. Newly developed methods using CO2 as internal tracer gas extrapolate CH4 emissions from few short measurements. However, both CO2 and CH4 emissions fluctuate during the day depending on the behavior and the post-feeding times questioning the validity of this method. Therefore, an innovative device was developed to monitor at a high rate CH4 and CO2 emission dynamics in order to investigate the link between CH4 dynamics and the animal behavior on pasture. Preliminary results showed the ability of the device to record differences in CH4:CO2 ratios and eructation frequencies according to the individual and the behavior. Results from complementary experiments in barn with animals fed contrasting diets regarding CH4 production (with and without linseed) and on pasture with different forage allowance will be presented in order to highlight how post-feeding time and grazing behavior impact CO2 and CH4 emission dynamics along the day. [less ▲]

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See detailNutritive value of three tropical forgae legumes and their influence on growth performance, carcass traits and organ weights of pigs
Kambashi Mutiaka, Bienvenu ULg; Kalala Bolokango, Gaetan ULg; Dochain, Denis et al

in Tropical Animal Health and Production (2016), 48(6), 1165-1173

The effects of tropical forage legumes on feed intake, growth performance and carcass traits were investigated in 16 groups of two Large White × Duroc pigs. The diets consisted of a commercial ... [more ▼]

The effects of tropical forage legumes on feed intake, growth performance and carcass traits were investigated in 16 groups of two Large White × Duroc pigs. The diets consisted of a commercial corn–soybean meal diet as the basal diet and three forage-supplemented diets. Four groups of control pigs received daily 4 % of body weight of the basal diet, and 12 groups of experimental pigs were fed the basal diet at 3.2 % of body weight completed with fresh leaves of one of the three forage legumes (Psophocarpus scandens, Stylosanthes guianensis and Vigna unguiculata) ad libitum. The study lasted 90 days. The in vitro digestion and fermentation of the forage legumes were also determined. The in vitro digestible energy content of the legumes was between 0.72 and 0.77 that of the basal diet (14.4 MJ/kg dry matter (DM)). V. unguiculata was the most digestible forage legume expected for crude protein digestibility. Feeding forage legumes lowered the dry matter intake by 4.5 to 9.6 % (P< 0.05), final body weight (P= 0.013), slaughter weight, average daily gain and hot carcass weight (P< 0.05) without affecting the feed conversion ratio (FCR), dressing percentage and back fat thickness. In conclusion, using forage to feed pig could be interesting in pig smallholder productionwith limited access to concentrate, as FCR was not significantly affected. [less ▲]

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See detailAgricultureIsLife or how to facilitate innovation in agriculture through multi-disciplinary research
Monty, Arnaud ULg; Garré, Sarah ULg; Bindelle, Jérôme ULg et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment (2016)

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See detailAdding mucins to an in vitro batch fermentation model of the large intestine induces changes in microbial population isolated from porcine feces depending on the substrate
Tran, Thi Hanh Tham ULg; Boudry, Christelle ULg; Everaert, Nadia ULg et al

in FEMS Microbiology Ecology (2016), 92(2), 13

Adding mucus to in vitro fermentation models of the large intestine showed that some genera, namely lactobacilli, are dependent on host-microbiota interactions and that they rely on mucosa layers to ... [more ▼]

Adding mucus to in vitro fermentation models of the large intestine showed that some genera, namely lactobacilli, are dependent on host-microbiota interactions and that they rely on mucosa layers to increase their activity. This study investigates whether this dependence on mucus is substrate-dependent and to which extend other genera are impacted by the presence of mucus. Inulin and cellulose were fermented in vitro by a fecal inoculum from pig in the presence or not of mucin-beads in order to compare fermentation patterns and bacterial communities. Mucins increased final gas production with inulin and shifted short-chain fatty acids molar ratios (P<0.001). QPCR analyses revealed that Lactobacillus spp. and Bifidobacterium spp. decreased with mucins, but Bacteroides spp. increased when inulin was fermented. A more in-depth community analysis indicated that the mucins increased Proteobacteria (0.55 vs. 0.25 %, P=0.013), Verrucomicrobia (5.25 vs. 0.03 %, P=0.032), Ruminococcaceae, Bacteroidaceae, and Akkermansia spp.. Proteobacteria (5.67 vs. 0.55 %, P<0.001) and Lachnospiraceae (33 vs. 10.4 %) were promoted in the mucuscompared to the broth, while Ruminococcaceae decreased. The introduction of mucins affected many microbial genera and fermentation patterns, but from PCA results, the impact of mucus was independent from the fermentation substrate. [less ▲]

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See detailIntra-cultivar potential of Desmanthus spp. as a greenhouse gas mitigation strategy for tropical livestock pastoral systems
Vandermeulen, Sophie ULg; Ramírez-Restrepo, Carlos Alberto; Singh, Sultan et al

Poster (2015, November)

Improved agricultural efficiency and reduction in the impacts of tropical livestock farming on habitat degradation require global approaches that enhance ruminant farming functionality in terms of feed ... [more ▼]

Improved agricultural efficiency and reduction in the impacts of tropical livestock farming on habitat degradation require global approaches that enhance ruminant farming functionality in terms of feed use efficiency, emissions and food security. This study evaluated the in vitro mitigation potential of the prostrate to erect, herbaceous Desmanthus spp. pasture legume adapted to semiarid clay soil land types in northern Australia. D. bicornutus, D. leptophyllus and D. virgatus were seasonally harvested from commercial plots by Agrimix Pty. Ltd. Samples of the legumes and the control Rhodes grass (Chloris gayana) using ruminal fluid from grazing Brahman (Bos indicus) steers were incubated in vitro (Ankom RF1 Technology) for 24, 48 and 72 h. Overall, the in vitro organic matter degradability (OMD) and methane production between Desmanthus species differed (P < 0.001). Compared to the control (0.656 ± 0.027 proportion of total OM) at 48 h of incubation, D. leptophyllus showed lower OMD (0.479 ± 0.016), while D. bicornutus (0.688 ± 0.016) and D. virgatus (0.619 ± 0.015) were different from each other, but similar to the control. Methane production (ml/g OM) was 15.7 ± 1.54, 3.7 ± 0.89, 12.0 ± 0.95 and 11.7 ± 0.95, respectively. It is suggested that the impact of these attributes may benefit household farmers in developing economies to expand productivity, improve livelihoods and meet the growing food consumption. Further analyses of the intra-cultivar characteristics of Desmanthus spp. will complement the design of sustainable and efficient interventions across tropical pastoral feeding systems, with a particular emphasis on large-scale grazing operations. [less ▲]

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