Isolation of five new monosomic alien addition lines of Gossypium australe F. Muell in G. hirsutum L. by SSR and GISH analyses

in Plant Breeding (2010), 130

Gossypium australe F. Muell (2n + 2x = 26) is a wild perennial species possessing agronomic useful traits that would be interesting to introgress into G. hirsutum L. (2n = 4x = 52), the main cultivated ... [more ▼]

Gossypium australe F. Muell (2n + 2x = 26) is a wild perennial species possessing agronomic useful traits that would be interesting to introgress into G. hirsutum L. (2n = 4x = 52), the main cultivated cotton species. To isolate monosomic alien addition lines (MAALs) of G. australe in G. hirsutum, the [2(G. hirsutum X G. australe) X G. hirsutum] pentaploid (2n = 5x = 65) was backcrossed as male parent to G. hirsutum. Analysis of 42 BC1 plants and seven alien addition lines, already available, with 150 SSR markers developed from G. hirsutum revealed a cross-species amplification rate of 100% and a polymorphism rate of 56%. Eighty polymorphic SSR markers generated 87 G. australe-specific loci that have been assigned by a hierarchical cluster analysis to 13 linkage groups corresponding to the 13 chromosomes of G. australe. Analysis by SSR markers and genomic in situ hybridization of the self-progeny of disomic alien addition lines, backcross progeny of the pentaploid, allowed the isolation of five new MAALs. [less ▲]

Introgression of the low-gossypol seed & high gossypol plant trait in upland cotton : Analysis of [(Gossypium hirsutum x G. raimondii)2 x G. sturtianum] trispecific hybrid and selected derivatives using mapped SSRs

in Molecular Breeding (2009)

Preferential transmission of Gossypium sturtanium chromosome fragments in the progeny of [(G. hirsutum x G. raimondii)2 x G. sturtanium] trispecies hybrid

in Proceedings of World cotton research conference IV (2008)

Optimization of a reliable, fast, cheap and sensitive silver staining method to detect SSR markers in polyacrylamide gels.

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2006), 10(2),

Molecular monitoring of the introgression in Gossypium hirsutum L. of G. sturtanium Willis genes controlling the "glanded-plant and glandless-seed" trait

Conference given outside the academic context (2004)

QTL analysis of the expression of the low-gossypol seed and high gossypol plant trait in the progeny of the Gossypium hirsutum x G. raimondii x G. sturtanium trispecific hybrid

Conference (2004)

Marquage moléculaire et suivi de l'introgression chez Gossypium hirsutum L. des gènes de G. sturtanium Willis contrôlant l'inhibition de la syntèse du gossypol uniquement dans la graine

Poster (2002)