Variability of Different Markers of Bone mineral Metabolism in Hemodialysis PAtients: Implication on the Evaluation of the Critical Difference between two Measurements

Poster (2012, July 17)

Human anti-animal interference in DiaSorin Liaison total 25(OH)-vitamin D assay: Towards the end of a strange story?

in Clinica Chimica Acta (2012), 413(3-4), 527-528

Interpretation of serum PTH concentrations with different kits in dialysis patients according to the KDIGO guidelines: importance of the reference (normal) values

in Nephrology Dialysis Transplantation (2012), 27

Background. The recommended target range for serum parathyroid hormone (PTH) in dialysis patients has changed from 150 to 300 pg/mL in the KDOQI guidelines to two to nine times the upper normal limit in ... [more ▼]

Background. The recommended target range for serum parathyroid hormone (PTH) in dialysis patients has changed from 150 to 300 pg/mL in the KDOQI guidelines to two to nine times the upper normal limit in the KDIGO ones. Although inclusion/exclusion criteria for the reference population are highly important, they are usually not mentioned in the commercial kits. In this study, we used the same reference population of vitamin D-replete normal subjects to establish reference values for 10 commercial PTH kits. We evaluated whether this may improve the classification of dialysis patients according to the KDIGO compared to the use of reference values proposed by the manufacturers. Methods. We measured serum PTH with 10 different kits in 149 haemodialysis patients, and 240 25-OH-vitamin D-replete (>75 nmol/L) individuals with an estimated glomerular filtration rate >60 mL/min/1.73 m2. Results. For the 10 kits, our upper normal limit was lower than those of the manufacturers. The difference was, however, variable from one kit to another. The two kits that yielded the lowest and the highest absolute concentrations classified differently 84/149 patients (56.4%) according to the KDOQI and 53/149 (36.2%) according to the KDIGO using the manufacturers’ normal value.Using our normal values significantly decreased the discrepancies with 24/149 patients (16.1%) being still classified differently. Taking the measurement uncertainty into consideration, 8% of the patients only remained differently classified by these two kits. Conclusions. Using the same vitamin-D-replete population to establish the reference range for 10 commercial PTH kits significantly improved the classification of haemodialysis patients according to the KDIGO target range. [less ▲]

New insights on the stability of the (1-84) PTH as determined with an automated 3rd generation PTH assay

in Clinical Chemistry (2011, October), 57(S10), 190

Automated Measurement of Total 25OH Vitamin D in the IDS iSYS System

Poster (2011, September 17)

Impact of the use of the Manufacturer's published reference range for PTH vs. the reference range established in the Laboratory for the classification of the haemodialyzed patients with the KDIGO Guidelines.

Poster (2011, July 28)

Neutrophil gelatinase-associated lipocalin (NGAL) determined in urine with the Abbott Architect or in plasma with the Biosite Triage? The laboratory's point of view

in Clinical Chemistry & Laboratory Medicine (2011), 49(2), 339-341

Le dosage urinaire et plasmatique du NGAL: étude analytique et implications pratiques pour le clinicien

in Néphrologie & Thérapeutique (2010, September), 6(5), 350

Analytical validation of the Abbott Architect urine NGAL

in Clinical Chemistry (2010, June), 56(S6), 96

Analytical validation of the BAP OSTASE on Liaison (DiaSorin).

in Clinical Chemistry & Laboratory Medicine (2010), 48(1), 67-72