References of "Beckers, Yves"
     in
Bookmark and Share    
Full Text
Peer Reviewed
See detailBehaviour and browse species selectivity of heifers grazing in a temperate silvopastoral system
Vandermeulen, Sophie ULg; Ramírez-Restrepo, Carlos Alberto; Marche, Christian et al

in Agroforestry Systems (in press)

In Belgium silvopastoral grazing systems associating trees and pasture are instrumental in enhancing dynamic trade-offs between ruminant farming and habitat improvement. A 130-day study was conducted in ... [more ▼]

In Belgium silvopastoral grazing systems associating trees and pasture are instrumental in enhancing dynamic trade-offs between ruminant farming and habitat improvement. A 130-day study was conducted in Belgium from April to September 2013 to evaluate the effects of browsing a combination of shrubs and trees (i.e. hedge) on the selective behaviour of cattle and to relate these observations to changes in forage nutritive value. Twelve Holstein dairy heifers (Bos taurus; 487 kg) were allocated to either a control ryegrass pasture (i.e. control pasture group; CPG) or a pasture plus unrestricted browsing (i.e. browsing group; BG) of a hedge composed of shrubs and trees. Behaviour and selectivity towards the woody species were recorded for 14h on a daily basis during 3 consecutive days over spring, early summer and late summer. Leaves and stems of woody species and faecal samples were collected during each season to analyse their nutritive value and predict the dry matter intake by means of near infrared reflectance spectroscopy. Integrating shrubs and trees along a pasture influenced the heifers’ behaviour and BG heifers spent 19.3, 5.9 and 5.4% of their time browsing during spring, and early and late summer, respectively (P < 0.001). This behaviour was correlated to the pre-grazing pasture biomass (r=0.50; P < 0.001). Compared with the summer seasons, the greater browsing activity in spring was associated with higher plant feeding value. Overall, the most ingested species were Carpinus betulus, Cornus sanguinea, Corylus avellana and Crataegus monogyna. It was concluded that cattle use a significant time budget for browsing on temperate ryegrass pasture but further research is required to investigate potential benefits of silvopastoral systems in Belgium. [less ▲]

Detailed reference viewed: 33 (16 ULg)
Full Text
Peer Reviewed
See detailMethane balance of an intensively grazed pasture and estimation of the enteric methane emissions from cattle
Dumortier, Pierre ULg; Aubinet, Marc ULg; Beckers, Yves ULg et al

in Agricultural and Forest Meteorology (2017), 232

The methane turbulent fluxes of an intensively grazed pasture were measured continuously from June 2012 to December 2013 at the Dorinne Terrestrial Observatory (DTO) in Belgium. During grazing periods ... [more ▼]

The methane turbulent fluxes of an intensively grazed pasture were measured continuously from June 2012 to December 2013 at the Dorinne Terrestrial Observatory (DTO) in Belgium. During grazing periods, the fluxes were dominated by enteric fermentation and were found to be strongly related to cow stocking density. In 2013, total emission from the pasture was found between 9 and 11 g CH4 m−2, 97% of which being emitted during grazing periods. Emission per LU (livestock unit) was estimated in a non-invasive way by integrating eddy covariance fluxes over large periods and by assuming a homogeneous average cattle disposition on the pasture. This estimate was compared to the one obtained during confinement periods, where cows were confined in a small part of the pasture. The emission per LU varied between 104 and 134 g CH4 LU−1 day−1 (13 and 17 g CH4 kg DMI−1), depending on the dataset and the computation method used. Diel course was characterized by two emission peaks, one in the morning and a larger one in the afternoon. For rest periods (no cattle on the pasture), small emissions were observed (median and mean values of 0.5 and 1.5 mg CH4 m−2 day−1, respectively). [less ▲]

Detailed reference viewed: 33 (15 ULg)
Full Text
Peer Reviewed
See detailLe profil en fibres alimentaires de légume modifie leur fermentation intestinale et la production d’acides gras à chaine courte dans modèle in vitro humain
Kalala Bolokango, Gaetan ULg; Kambashi Mutiaka, Bienvenu ULg; Njeumen, Patrick et al

Poster (2016, December 01)

La modulation du microbiote intestinal induite par les fibres alimentaires (DF)a des conséquences positives sur la santé, notamment sur l’obésité et ses comorbidités. La nature et l’ampleur des ... [more ▼]

La modulation du microbiote intestinal induite par les fibres alimentaires (DF)a des conséquences positives sur la santé, notamment sur l’obésité et ses comorbidités. La nature et l’ampleur des changements dépendent du type de fibres.Les légumes sont des sources privilégiées de DF, certaines espèces et variétés fournissant davantage de DF solubles (SDF), d’autres étant plus riches en DF insolubles (IDF). Contrairement aux SDF, les IDF sont souvent considérées comme peu fermentescibles, ne contribuant que faiblement à une modulation de l’écophysiologie intestinale. En opposition à cette hypothèse, pour explorer si l’impact potentiel sur la santé intestinale des légumes varie suivant leur nature, des légumes présentant des teneurs et des profils en DF différents ont été comparés dans un modèle in vitro de fermentation colique. [less ▲]

Detailed reference viewed: 21 (5 ULg)
Full Text
See detailCarbon balance of an intensively grazed grassland in southern Belgium
Gourlez de la Motte, Louis ULg; Jérôme, Elisabeth; Mamadou, Ossénatou et al

in Agricultural and Forest Meteorology (2016), 228-229

Detailed reference viewed: 24 (9 ULg)
Full Text
See detailLes xylanases : hier et demain ?
Beckers, Yves ULg

Conference given outside the academic context (2016)

Detailed reference viewed: 54 (1 ULg)
Full Text
See detailNourrir à l’herbe : principales règles nutritionnelles pour réussir
Beckers, Yves ULg

Conference given outside the academic context (2016)

Detailed reference viewed: 21 (0 ULg)
Full Text
See detailImpact of high-wheat bran diet on sows’ microbiota, performances and progeny’s growth and health
Leblois, Julie ULg; Bindelle, Jérôme ULg; Dehareng, Frédéric et al

Conference (2016, September 02)

Finding alternatives to antimicrobial growth promoters is part of the goal of improving sustainability in pig production. Dietary fibres are considered as health-promoting substances acting on pigs’ ... [more ▼]

Finding alternatives to antimicrobial growth promoters is part of the goal of improving sustainability in pig production. Dietary fibres are considered as health-promoting substances acting on pigs’ microbiota. This study aimed to investigate whether the enrichment of sows’ diet with high levels of wheat bran (WB) could impact the performances of sows and piglets’ health. Seven sows were fed a control diet (CON) and 8 sows a WB diet from day 43 of gestation (WB 240 g/kg DM) until the end of the lactation period (WB 140 g/kg DM). Diets were formulated to be iso-energetic and iso-nitrogenous by changing the proportions of some ingredients. Faeces were sampled at different time points (before treatment, during treatment: in gestation and lactation) to determine microbiota composition (sequencing with Illumina MiSeq). Milk was sampled weekly to determine lactose, fat and protein concentration by mid-infrared technology and IgA and IgG contents by ELISA. Before weaning (d26-27), piglets were euthanized, intestinal contents and tissues sampled for further analyses. Zootechnical performances of sows and piglets were recorded. Statistical analyses were performed using the SAS MIXED procedure and repeated measurements. Treatment never impacted piglets’ weight (P=0.51). Sows’ ingestion during the lactation period was comparable between both treatments until the last 4 days of lactation where the percentage of target ingestion was significantly (P<0.001) lower for the WB (66%) compared to the CON group (89%). No effect on sows’ backfat and weight changes was observed. An increased abundance of Lactobacillus spp. in feces of the WB group was observed in gestation before and after diet change (8.8% vs 15.1% of total bacteria). However, for the overall genera changes between treatments, it only seems to occur for minor groups of bacteria. Milk protein, fat, IgG and IgA were not affected by treatment, but a time-effect (P<0.001) was observed while treatment impacted (P<0.05) lactose content. In conclusion, sows’ performances were not affected by the high WB diet and more research on the piglets’ samples is foreseen. [less ▲]

Detailed reference viewed: 45 (7 ULg)
Full Text
Peer Reviewed
See detailStrategies of the Walloon dairy breeders faced to the uncertain dairy future
Dalcq, Anne-Catherine ULg; Dogot, Thomas ULg; Soyeurt, Hélène ULg et al

Conference (2016, September 01)

This study observes the strategies, and their determinants, of the Walloon dairy breeders faced to the post quota perspective through the realization of 245 surveys, conducted from November 2014 to ... [more ▼]

This study observes the strategies, and their determinants, of the Walloon dairy breeders faced to the post quota perspective through the realization of 245 surveys, conducted from November 2014 to February 2015. Three kinds of strategical variables were defined and related to the evolution of milk production (MP) [the breeders who increase MP (HighMP) vs. keep constant MP (ConstantMP) vs. stop MP]; the valorisation of MP [alternative (ValMP) vs. classical] and the diversification of activities [with (DivMP) vs. without such activities]. The relationships between the chosen strategies and the quantitative technical variables were studied using generalized linear models. The independence between qualitative technical variables and the strategical variables was tested using Chi Square test. HighMP and ConstantMP bredeers represent 38.4% and 53.9% of respondents, respectively. HighMP breeders were significantly more declared as legal entity (p-value = 0.03), had more family members on the farm (p-value<0.01), larger agricultural area in property (p-value = 0.03) and higher MP quota (p-value = 0.01) compared to ConstantMP breeders. Only 9.8% of respondents decide to valorise differently MP. ValMP breeders tend to have more employees (p-value = 0.08) and an agricultural area less fragmented (p-value = 0.07) than classical breeders. A total of 7.8% of respondents decide to develop other activities. DivMP breeders tend to have more employees (p-value = 0.10), more agricultural area in property (p-value = 0.03) and a more recent year of installation (p-value < 0.01). Finally, 44.9% of ConstantMP breeders do not want to start an alternative valorisation of MP and diversify their activities. In conclusion, a relationship exists between, amongst others, the legal status, workforce available, characteristics of the agricultural area, the dairy production and the strategy chosen by the Walloon dairy breeders. [less ▲]

Detailed reference viewed: 27 (5 ULg)
Full Text
See detailL'acétonémie : un bien ou un mal nécessaire pour la vache laitière ?
Beckers, Yves ULg

Conference given outside the academic context (2016)

Detailed reference viewed: 20 (2 ULg)
Full Text
See detailImpact of cooking and species on intestinal fermentation patterns of vegetables in a humanized in vitro model of the gastro-intestinal tract
Kalala Bolokango, Gaetan ULg; Kambashi Mutiaka, Bienvenu ULg; Njeumen Lemotio, Georges Patrick ULg et al

Poster (2016, June 21)

Obesity and associated pathologies have dramatic consequences on patients’lives as well as high societal costs. Because of the role of intestinal dysbiosis and microbiota make-up on the pathogenesis of ... [more ▼]

Obesity and associated pathologies have dramatic consequences on patients’lives as well as high societal costs. Because of the role of intestinal dysbiosis and microbiota make-up on the pathogenesis of obesity, several strategies such as eating prebiotics and dietary fibre supplements are being investigated to reshape the intestinal microbial communities of obese patients. Beyond supplement, dietary fibre is supplied through plant ingredients in the meals. In the framework of the multidisciplinary research project Food4Gut, the use of vegetables rich in specific targeted dietary fiber, namely fructans, is being scrutinized for its ability to induce positive changes in the intestinal ecophysiology. Because expected effect might differ according the content in dietary fibre and fructans, the soluble:insoluble ratio, as well as the cooking of the vegetables, the fermentation patterns of several vegetables are being investigated in an dual in vitro model combining enzymatic hydrolysis to an in vitro fermentation step using faecal inoculums from humans, to evaluate the performance of gut microbiota, modulation of metabolic functions. Six vegetables were sampled in triplicates (N=3) and steamed for 20 to 30 min.: Jerusalem artichoke, salsify, asparagus, pumpkin, fennel and swede. They were chosen because they display a variety of contents in fructans, soluble (SDF) and insoluble dietaryfibre (IDF). Steamed vegetable samplesand burgers from local fast food restaurants (negative control) were hydrolyzed in vitro why porcine pepsin and pancreatin to mimic digestion in the upper gut and indigested fiber residues were recover using a 6kDa dialysis membrane.Subsequently, in vitro fermentation is being run with independent fecal inoculums from obese and lean patients (N=4). Fermentation kinetics over 24h as well as short-chain fatty acid production and profiles will be compared according to the individual donor and the vegetable species and multivariate analysis will be used to explore the relationships between donor, vegetable species and composition and fermentation patterns. [less ▲]

Detailed reference viewed: 56 (28 ULg)
Full Text
Peer Reviewed
See detailEffects of protein source and cooking procedure on intestinal microbiota and on fermentation end-products in rats
POELAERT, Christine ULg; Despret, Xavier; Portetelle, Daniel ULg et al

Poster (2016, June)

Animal and plant proteins are major proteins sources in the human diet. After their enzymatic degradation in the upper gastro-intestinal tract, the undigested fraction of these proteins is available for ... [more ▼]

Animal and plant proteins are major proteins sources in the human diet. After their enzymatic degradation in the upper gastro-intestinal tract, the undigested fraction of these proteins is available for fermentation by the microbiota of the large intestine leading to the production of short-chain fatty acids (SCFA), branched-chain fatty acids (BCFA), ammonia, biogenic amines, sulphur metabolites, phenols and indoles. As some of these compounds have genotoxic and cytotoxic effects, protein fermentation is considered as detrimental to the host’s epithelial health. BCFA are usually used as a marker of intestinal protein fermentation. We studied in vivo the impact of proteins from animal and plant origin, raw or after a cooking procedure, on the composition of gut microbiota and on fermentation end-products. Weanling rats were used as models of the human gut microbiota. Eight experimental diets were formulated with beef meat (Longissimus dorsi), chicken meat (Pectoralis major), white pea beans (Phaseolus vulgaris), soybeans (Glycine max), used raw and cooked, as sole source of protein in the diet. One casein diet was used as control. All diets, formulated to contain 15% of raw protein, were given to seven rats for four weeks. After euthanasia, caecal contents were collected. Pyrosequencing analyses (Roche 454 GS Junior Genome Sequencer) were performed to study the microbial composition. SCFA and BCFA were measured using HPLC (Waters 2690). Microbial composition in the caecum is associated to the type of dietary protein and to the cooking procedure applied. The proportion of BCFA in the caecal content is mainly affected by the type of protein. So BCFA represent respectively 04-06% and 35-44% of total SCFA with diets based on plant and on animal proteins. In conclusion, both the type of protein and the cooking procedure could impact the gut microbiota in terms of composition and of fermentative capacity. [less ▲]

Detailed reference viewed: 54 (14 ULg)
Full Text
See detailChangements climatiques : les impacts des ruminants et quels leviers d'action
Beckers, Yves ULg

Scientific conference (2016, May 02)

Detailed reference viewed: 39 (9 ULg)
Full Text
See detailImpact of wheat bran supplementation to sows on their milk quality, their performances and their progeny’s
Leblois, Julie ULg; Bindelle, Jérôme ULg; Dehareng, Frédéric et al

Conference (2016, April 15)

Detailed reference viewed: 40 (9 ULg)
Full Text
See detailContribution à l'optimisation technico-économique des élevages laitiers en Wallonie : l'intervalle vêlage
Dalcq, Anne-Catherine ULg; Beckers, Yves ULg; Dogot, Thomas ULg et al

Conference given outside the academic context (2016)

Au cours des dernières décennies, l’intervalle vêlage des vaches laitières a eu tendance à s’allonger au niveau mondial, européen et belge. Les causes sont multiples : évolution du système de production ... [more ▼]

Au cours des dernières décennies, l’intervalle vêlage des vaches laitières a eu tendance à s’allonger au niveau mondial, européen et belge. Les causes sont multiples : évolution du système de production laitière, augmentation du niveau de production,… Les conséquences sont nombreuses également mais se traduisent-elles par un impact économique pour l’éleveur laitier ? La recherche présentée aujourd’hui se base sur près de 1800 bilans comptables de 400 exploitations laitières, fournis par le service technico-économique de l’Association Wallonne de l’Elevage, entre 2007 et 2014, pour déterminer l’impact économique de la durée de l’intervalle vêlage et définir l’optimum technico-économique de ce paramètre de management. Faut-il garder en tête « le veau par vache et par an » ou est-il intéressant économiquement d’allonger la période entre deux vêlages pour une même vache ? L’étude révèle qu’il y a bien une relation entre l’intervalle vêlage et les résultats économiques d’une exploitation. De plus, il n’y aurait pas un seul optimum d’intervalle vêlage mais plusieurs, dépendant du type d’exploitation et plus particulièrement du mode d’alimentation. L’optimum de l’intervalle vêlage a tendance à être plus court pour les exploitations à alimentation plutôt intensive et plus long pour les exploitations à alimentation plutôt extensive. Cependant il ne s’agit que de tendances observées, un travail plus approfondi doit encore être réalisé pour confirmer ces tendances et définir des objectifs plus précis à poursuivre pour maximiser la rentabilité de son exploitation. [less ▲]

Detailed reference viewed: 124 (3 ULg)
Full Text
See detailIn vitro evaluation of protein precipitation capacity of temperate browse species
Vandermeulen, Sophie ULg; Leblois, Julie ULg; Ramírez-Restrepo, Carlos Alberto et al

Poster (2016, February 05)

European agri-environmental policies are promoting the establishment of shrubs and trees on grasslands. The use of browse as fodder requires knowledge on their nutritive value since intensive production ... [more ▼]

European agri-environmental policies are promoting the establishment of shrubs and trees on grasslands. The use of browse as fodder requires knowledge on their nutritive value since intensive production systems are still relying on expensive and environment-costing protein sources. However, information on the influence of temperate condensed tannins (CT)-containing browse forage on rumen protein metabolism is elusive. The study aimed to assess the protein precipitation capacity (PPC) of 10 temperate browse species and establish the correlation between PPC values and plants CT content. PPC of foliage of 3 individuals per woody plants was measured using 2 model proteins: bovine serum albumin (BSA) and casein. The N content in protein solutions (4.6g/L; pH=6.8) was determined before and after adding each forage sample. Extractable CT concentration was quantified by spectrophotometry. The PPC varied across plant species (P<0.001). Corylus avellana had the highest ability to precipitate casein (52.4%). In contrast, the BSA precipitation (18.3%) of this plant was similar to Cornus sanguinea (12.7%), Quercus robur (12.1%) and Crataegus monogyna (11.0%). CT content ranged from 1.4 in Fraxinus excelsior to 82.7g/kg of depigmented sample in Corylus avellana (P<0.001) and was correlated to BSA (r=0.70; P<0.001) and casein PC (r=0.51; P<0.01). It was concluded that woody species could play a significant role in modifying protein metabolism, but further in vivo trials are required. [less ▲]

Detailed reference viewed: 52 (11 ULg)
Full Text
See detailLINKING CATTLE GRAZING BEHAVIOR TO METHANE AND CARBON DIOXIDE DYNAMICS
Blaise, Yannick ULg; Lebeau, Frédéric ULg; Andriamandroso, Andriamasinoro ULg et al

in Communications in Agricultural and Applied Biological Sciences (2016, February), 81(1), 107-112

Various methods are presently used to measure methane (CH4) emissions of ruminants on pasture. Those measurements are essential to evaluate nutritional strategies to mitigate enteric CH4 emissions as well ... [more ▼]

Various methods are presently used to measure methane (CH4) emissions of ruminants on pasture. Those measurements are essential to evaluate nutritional strategies to mitigate enteric CH4 emissions as well as addressing the selection of low producing individuals. On pasture and in the barn, variations in CH4 emissions are observed depending on the time of the day. However, no studies have been made to link these diurnal fluctuations to behavioural phases, especially on pasture. The aim of this study was to understand the individual dynamics of CH4 production and their links to the grazing behaviour. For this purpose, a new tool was specifically developed. Five red-pied dry cows were equipped with infrared CH4 and carbon dioxide (CO2) sensors measuring concentrations in the exhaled air at 4 Hz. The animals were equipped with a heart rate belt (HR) and motion sensors to detect their feeding behaviours (grazing vs. rumination) for periods of 8 h/d. Wind speed (WS) was also monitor to verify interference with sampled gas concentrations. Results showed that using the CH4:CO2 ratio reduced the interference with WS that was observed on raw CH4 and CO2 concentration signals. CH4:CO2 ratio average over 5 min periods indicated that CH4 emissions were lower during grazing than rumination (P<0.01). The eructation frequency during grazing (0.48 eructation/min, P<0.01) was also lower than during rumination (0.65 eructation/min). HR was higher during grazing that rumination. Because HR is usually linked to metabolic CO2 production intensity, hence influencing the denominator of the CH4:CO2 ratio, further investigation should focus on the quantification of changes in fermentative and metabolic CO2 emissions along the day to estimate total CH4 production more accurately and the relationship between CH4 emissions patterns and post-feeding times. [less ▲]

Detailed reference viewed: 257 (47 ULg)
Full Text
See detailOUTDOOR MEASUREMENT OF CATTLE METHANE EMISSIONS USING THE EDDY-COVARIANCE TECHNIQUE IN COMBINATION WITH GEOLOCALIZATION DEVICES
Dumortier, Pierre ULg; Andriamandroso, Andriamasinoro ULg; Aubinet, Marc ULg et al

Poster (2016, February)

Methane emissions account for 8% of the EU-15 GHG emissions and livestock generates approximately half of these emissions [1]. In order to improve emissions reporting and properly test mitigation options ... [more ▼]

Methane emissions account for 8% of the EU-15 GHG emissions and livestock generates approximately half of these emissions [1]. In order to improve emissions reporting and properly test mitigation options, techniques for measuring methane emissions from cattle must be developed and adapted to each management system. Among available micrometeorological methods, the use of eddy-covariance is still in its infancy [2] and its relevance and robustness for cattle flux estimation has still to be proved. On one hand, it is well adapted to seasonal grazing systems, is non-invasive, needs little animal handling and allows detection of daily emission patterns. On the other hand, it has the drawback of requiring cattle geo-localization and long periods of measurements (typically one month). In this study, we combined measured CH4 fluxes with a footprint model [3] and cattle positions (GPS devices) over several one-month campaigns at key periods in the grazing season in order to obtain CH4 emissions per cow at herd scale. Accelerometers were also added to the system for behaviour detection, opening the possibility of linking emissions to feeding behaviour. Measurements were performed and are still ongoing at the Dorinne Terrestrial Observatory in 2014/2015. The first campaign provided a mean emission per cow of 65±6 kg CH4.LSU-1.year-1. Cattle emission pattern was tightly linked with behaviour pattern, emissions being higher during and shortly after grazing (i.e. at dawn and dusk). Uncertainties linked to the method will be discussed and quantified (footprint model validity, geo-localization precision, eddy covariance corrections and filtering specificities linked to CH4 measurements). Compilation of data from multiple campaigns will allow quantification of the effects of forage quality, animal weight and lactating state on emissions per cow. [less ▲]

Detailed reference viewed: 72 (14 ULg)
Full Text
See detailReview of shrubs and trees in intensive ruminant systems in temperate areas
Vandermeulen, Sophie ULg; Ramìrez-Restrepo, Carlos Alberto; Beckers, Yves ULg et al

Conference (2016, January)

Using shrubs and trees as forage for ruminants is common in extensive production systems in the tropics, as well as the Mediterranean region and mountain areas. In temperate Europe, the intensification of ... [more ▼]

Using shrubs and trees as forage for ruminants is common in extensive production systems in the tropics, as well as the Mediterranean region and mountain areas. In temperate Europe, the intensification of agriculture led to a decline in the numbers of woody perennials on farmlands. A review of the potential uses of shrubs and trees in temperate intensive systems shows that this concept is rather recent. Few studies have been investigating the potential outputs and limitations of shrubs and trees forage in production systems, while in Belgium and other European countries, agro-environmental policies are promoting the establishment of hedgerows and woody strips that provide shelter to animals against variable climate conditions. Furthermore, it has been found that ruminant species browse the plants, or alternatively, the forage is harvested and fed fresh or preserved as hay, silages or pellets. In both cases, consequences on feed intake control, woody plant survival, dry matter (DM) production and forage quality in terms of crude protein content reduction have been documented. In addition, depending on the plant species and the preservation method, bio-active plant metabolites such as condensed tannins (CT) are also present in the range of less than 1 to more than 100 g/kg foliage DM. Overall, CT may reduce ruminal N degradation, methanogenesis and nematode parasites infestation, while enhancing microbial-protein synthesis, feed use efficiency and systemic animal physiology. Planting shrubs and trees into the agricultural landscape (i.e. silvopastoral system) can further improve biodiversity and environmental services. Nevertheless, agronomic practices, farm management or environmental policy limitations may reduce the use of this fodder resource. Therefore, although silvopastoral systems seem promising in temperate ruminant systems, the current knowledge to their introduction and efficient management need to be cautiously considered. [less ▲]

Detailed reference viewed: 129 (20 ULg)
Full Text
Peer Reviewed
See detailRelationship between thiamine and subacute ruminal acidosis induced by a high-gran diet in dairy cows
Pan, Xiaohua; Yang, L.; Xue, F.G. et al

in Journal of Dairy Science (2016), 99(11), 8790-8801

Two experiments were conducted to reveal the effects of grain-induced subacute rumen acidosis (SARA) on thiamine status in blood and rumen fluid in dairy cows. In both experiments, 6 multiparous, rumen ... [more ▼]

Two experiments were conducted to reveal the effects of grain-induced subacute rumen acidosis (SARA) on thiamine status in blood and rumen fluid in dairy cows. In both experiments, 6 multiparous, rumen-fistulated Holstein dairy cows were used in a 2-treatment, 2-period crossover design. Each experimental period consisted of 21 d (total of 42 d). Experiment 1 was to investigate the effects of SARA on thiamine status in blood and rumen fluid. Treatments were either control (20% starch, dry matter basis) or SARA-inducing diet (SAID, 33.2% starch, dry matter basis). In experiment 2, the effects of dietary thiamine supplementation on attenuating SARA and ruminal fermentation characteristics in dairy cows were studied. All cows received the same SAID diet during the whole experimental period; treatments were with or without thiamine (180 mg of thiamine/kg of dry matter intake). In both experiments, rumen fluid samples were collected at 0, 3, 6, 9, and 12 h after morning feeding on d 21 and 42 of the experiments for measurement of pH, thiamine, volatile fatty acid, and lactate contents. Peripheral blood was also collected at 3 h after morning feeding on d 21 and 42 to measure thiamine, carbohydrate metabolites, and enzyme activities. In experiment 1, cows fed the SAID diet had lower ruminal and plasma thiamine concentrations and higher lactate than cows fed the control diet. The ruminal thiamine contents were positively related to pH and the concentrations of acetate in the rumen, and negatively correlated with the lactate contents. Experiment 2 demonstrated that ruminal pH and the concentrations of thiamine, acetate, and total volatile fatty acids in the rumen were increased, whereas ruminal lactate contents were reduced by thiamine supplementation. The concentrations of lactate and the activity of lactate dehydrogenase in blood were reduced in the thiamine supplemented group, and the opposite was true for the nonesterified fatty acids and α-ketoneglutarate dehydrogenase contents. In conclusion, the thiamine status was affected by SARA in dairy cows and ruminal infusion of thiamine could helpattenuate SARA by improving the proportions of ruminal volatile fatty acids and reducing lactate contents in rumen fluid and blood. [less ▲]

Detailed reference viewed: 29 (8 ULg)