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See detailEffect of bovine colostrum intake on growth, reproductive parameters and survival in red kids
Harouna, Abdou; Marichatou, Hamani; Beckers, Jean-François ULg et al

in Journal of Animal Physiology & Animal Nutrition (in press)

The aim of this study is to evaluate the efficacy of frozen Azawak colostrum supplementation on body weight (BW), average daily gain (ADG), reproductive parameters (mean age at first parturition ... [more ▼]

The aim of this study is to evaluate the efficacy of frozen Azawak colostrum supplementation on body weight (BW), average daily gain (ADG), reproductive parameters (mean age at first parturition, fertility, fecundity, prolificacy) and mortality rate among red kids. The study was conducted at the goat farm secondary centre of Maradi in Niger from September 2010 to September 2011. The control animals (n = 20) were left with their mother, while the treatment animals (n = 20) received in addition 50 ml/animal/day of bovine colostrum at birth and 15 ml/animal/day from d2 to d15. Weight was measured weekly from birth to d365. Mortalities were also recorded over the same period. For reproductive parameters, observations began at weaning (d197). Growth rate was higher (p < 0.001) in supplemented animal, and the treatment effects on ADG were observed up to 150 day after the end of supplementation. A similar long-lasting trend was also observed in relation to the mortality rate (25% for ColG vs. 55% for ConG; p = 0.05). The age at first kidding tended to be lower in the treated group (13.8 0.7 vs. 14.1 0.8 month; p < 0.1). In conclusion, mild bovine colostrum supplementation induces a long-lasting positive impact on growth rate and to a lower extent on reproduction parameters and survival rate. [less ▲]

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See detailChemical composition of colostrum from Azawak cow in Niger compared with meta-analytical data
Abdou, Harouna; Marichatou, Hamani; Beckers, Jean-François ULg et al

in Animal Genetic Resources Information = Bulletin d'Information sur les Ressources Génétiques Animales = Boletin de Informacion sobre Recursos Geneticos Animales (in press)

This study aimed at comparing data obtained from Azawak zebu colostrum with literature data. The comparison was performed by a meta-analytical approach. Colostrum samples were hand-collected after 5 h ... [more ▼]

This study aimed at comparing data obtained from Azawak zebu colostrum with literature data. The comparison was performed by a meta-analytical approach. Colostrum samples were hand-collected after 5 h from 7 Azawak cows at calving between August 27, to September 10, 2009 in the Sahel. For data from literature, twenty one (21) references were identified in the following analytical databases: PubMed, Science Direct, Google scholar, Collection from University of Liege. The references were selected according to the following two criteria: i) only studies reported on bovine colostrum were used irrespective of breeds, and ii) among the selected studies, those not providing complete information to allow meta-analytical calculation were excluded. Samples were analyzed for immunoglobulins (IgG, IgM, IgA), lactoferrin, and chemical composition (dry mater, protein, fat, lactose, ash, Ca, P, NA, K, Mg). The mean levels of IgG, IgM, dry matter, protein and fat for Azawak cows were lower (P ˂ 0.001) than those obtained in other breeds; however colostrum from the Azawak was higher in IgA but the difference was not significant. For lactose and ash, mean values for Azawak cows were higher (P ˂ 0.001) than those from the literature. Contents of Ca, P, Na and Mg in Azawak bovine colostrum were significantly higher (P ˂ 0.001) than the mean levels in form the literature data. In conclusion, the colostrum from Azawak cows appears to be lower in most immunoglobulins, in fat and in protein than the values reported in the literature, but higher in lactose and minerals. This could be an adaptation to sahelian contraints. [less ▲]

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See detailInvestigation of pregnancy-associated glycoproteins (PAGs) by means of an enzymoimunoassay (ELISA) sandwich kit for pregnancy monitoring in sheep
El Amiri, Bouchra; Delahaut, Philippe; Colemans, Yves et al

in Chentouf, M; López-Francos, A; Bengoumi, M (Eds.) et al Options méditerranéennes : Series A (2014)

The aim of the present study was to investigate the use of a PAGs ELISA-Sandwich kit (Ret. code E.G.7. CER. Marloie, Belgium) for both early pregnancy diagnosis (in Sardi sheep) and the pregnancy follow ... [more ▼]

The aim of the present study was to investigate the use of a PAGs ELISA-Sandwich kit (Ret. code E.G.7. CER. Marloie, Belgium) for both early pregnancy diagnosis (in Sardi sheep) and the pregnancy follow up (in Boujaâd and Boujaâd x D'man sheep). ln Sardi breed, plasma samples were obtained from pregnant ewes (n = 17) from day 18 to 30 of gestation at 2 days interval. ln Boujaâd (n = 8) and Boujaâd x D'man (n = 20) the blood samples were weekly collected from the first week of gestation till the 4th week after lambing. The PAG concentrations were determined by a sandwich-ELISA based on purified bovine PAG (boPAG-67 kDa) as a standard, the antiserum raised against caprine PAG (caPAG-55+62 kDa) as a capture antibody (1/40000) and antiserum raised against purified PAG from buffalo (AS 859) as detection antibody (1/32 000). The Avidin-HRP and TMB were used to reveal reactions. Ewes were assumed to be pregnant when PAG concentrations were higher than 0.8 ng/ml. Results showed that in Sardi sheep it is possible to detect ail ewes as pregnant as early as on 24 days of gestation. ln Boujaâd and Boujaâd x D'man the PAG concentrations reached the higher values just before lambing. This value is higher in ewes carrying more than one lamb than in those carrying a single lamb. To conclude, the present study shows that the ELISA kit used for early pregnancy diagnosis in sheep as weil as for pregnancy fellow up could be a good alternative to the radioimmunoassay RIA in countries where this last technique is hard to set up. [less ▲]

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See detailIn vitro embryo production in goats: slaughterhouse and laparoscopic ovum pick up 2 (LOPU) derived oocytes have different kinetics and requirements regarding maturation 3 media
Souza-Fabjan, JM; Locatelli, Y; Duffard, N et al

in Theriogenology (2014), 81

A total of 3427 goat oocytes were used in this study in order to identify possible 24 differences during in vitro embryo production from slaughterhouse or laparoscopic ovum 25 pick up (LOPU) oocytes. In ... [more ▼]

A total of 3427 goat oocytes were used in this study in order to identify possible 24 differences during in vitro embryo production from slaughterhouse or laparoscopic ovum 25 pick up (LOPU) oocytes. In Experiment 1, one complex, one semi defined and one simplified 26 in vitro maturation (IVM) media were compared using slaughterhouse oocytes. In 27 Experiment 2, we checked the effect of oocyte origin (slaughterhouse or LOPU) on the 28 kinetics of maturation (18 vs. 22 vs. 26 h) when submitted to semi defined or simplified 29 media. In Experiment 3, we determined the differences in embryo development between 30 slaughterhouse and LOPU oocytes when submitted to both media and then to in vitro 31 fertilization (IVF) or parthenogenetic activation (PA). Embryos from all groups were vitrified 32 and their viability evaluated in vitro after thawing. In Experiment 1, no difference (P>0.05) 33 was detected among treatments for maturation rate (MII; 88% on average), cleavage (72%), 34 blastocyst from the initial number of cumulus oocyte complexes (COC; 46%) or from the 35 cleaved ones (63%), hatching rate (69%) and the total number of blastomeres (187). In 36 Experiment 2, there was no difference of MII rate between slaughterhouse oocytes cultured 37 for 18 or 22 h, whereas the MII rate increased significantly (P<0.05) between 18 and 22 h for 38 LOPU oocytes in the simplified medium. Moreover, slaughterhouse oocytes cultured in 39 simplified medium matured significantly faster than LOPU oocytes at 18 and 22 h (P<0.05). 40 In Experiment 3, cleavage rate was significantly greater (P<0.001) in all four groups of 41 embryos produced by PA than IVF. Interestingly, PA reached similar rates for slaughterhouse 42 oocytes cultured in both media, but improved (P<0.05) cleavage rate of LOPU oocytes. 43 Slaughterhouse oocytes had acceptable cleavage rate after IVF (~67%), whereas LOPU 44 oocytes displayed a lower one (~38%), in contrast to cleavage after PA. The percentage of 45 blastocysts in relation to cleaved embryos was not affected by the origin of the oocytes 46 (P>0.05). Therefore, slaughterhouse oocytes developed a greater proportion of blastocysts than LOPU ones, expressed as the percentage of total COC entering to IVM. Vitrified-thawed 48 blastocysts presented similar survival and hatching rates between the oocyte origin, media or 49 method of activation. In conclusion, slaughterhouse and LOPU derived oocytes may have 50 different IVM kinetics and require different IVM-IVF conditions. Although the IVM and IVF 51 systems still need improvements in order to enhance embryo yield, the in vitro development 52 (IVD) step is able to generate good quality embryos from LOPU derived oocytes. [less ▲]

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See detailIdentification of pregnancy-associated glycoproteins and alpha-fetoprotein in fallow deer (Dama dama) placenta
Bériot, Mathilde ULg; Tchimbou Njanjo, Aline Flora ULg; Barbato, Olimpia et al

in Acta Veterinaria Scandinavica (2014), 56(4), 1-11

Background: This paper describes the isolation and characterization of pregnancy-associated glycoproteins (PAG) from fetal cotyledonary tissue (FCT) and maternal caruncular tissue (MCT) collected from ... [more ▼]

Background: This paper describes the isolation and characterization of pregnancy-associated glycoproteins (PAG) from fetal cotyledonary tissue (FCT) and maternal caruncular tissue (MCT) collected from fallow deer (Dama dama) pregnant females. Proteins issued from FCT and MCT were submitted to affinity chromatographies by using Vicia villosa agarose (VVA) or anti-bovine PAG-2 (R#438) coupled to Sepharose 4B gel. Finally, they were characterized by SDSPAGE and N-terminal microsequencing. Results: Four distinct fallow deer PAG (fdPAG) sequences were identified and submitted to Swiss-Prot database. Comparison of fdPAG with PAG sequences identified in other ruminant species exhibited 64 to 83% identity. Additionally, alpha-fetoprotein was identified in fetal and maternal tissues. Conclusion: Our results demonstrate the efficacy of VVA and bovine PAG-2 affinity chromatographies for the isolation of PAG molecules expressed in deer placenta. This is the first report giving four specific amino acid sequences of PAG isolated from feto-maternal junction (FCT and MCT) in the Cervidae family. [less ▲]

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See detailEffect of bovine colostrum administration on plasma protein profile, growth, and survival in Red kid.
Abdou, Harouna ULg; Marichatou, Hamani; Beckers, Jean-François ULg et al

in Small Ruminant Research (2013), 117

This study evaluated the effect of colostrum from Azawak cows on plasma protein profile, 24 growth, and survival in Red kids from Niger. Forty (40) newborn kids were allocated to one 25 of two treatment ... [more ▼]

This study evaluated the effect of colostrum from Azawak cows on plasma protein profile, 24 growth, and survival in Red kids from Niger. Forty (40) newborn kids were allocated to one 25 of two treatment-groups: control (free access to water and the mother) and colostrum (free 26 access to water and the mother, but with additional 50 mL of colostrum/animal/day of birth 27 and 25 mL/animal/day from the 2nd to 15th day of age). Blood samples were collected into 28 EDTA vacutainer tubes by jugular puncture at 10 and 30 days of age. Total protein was 29 quantified by the Biuret method. The agarose gel electrophoresis was used to determine the 30 serum levels of albumin, -globulin, β1-globulin, β2-globulin, γ-globulin and the 31 albumin/globulin ratio. The animals from the colostrum group showed higher body weight 32 and average daily gain when compared to the control group (P < 0.001). The average 33 concentration in protein at the both sampling times reached higher values in the colostrum 34 than in the control group. At D10, the colostrum group tended (P < 0.07) to show higher 35 levels of -globulin and had higher values (P < 0.05) for β1-globulin. At D30, total protein 36 and β2-globulins were higher in colostrum group. Colostrum from Azawak cows seems to 37 have positive effects on some plasma proteins levels and on growth rate in Red kids. [less ▲]

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See detailVitrification of immature equine oocytes
Gatez, Carine ULg; Ectors, Fabien ULg; Farnir, Frédéric ULg et al

Poster (2013, October 11)

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See detailCharacteristics of Pregnancy-Associated glycoprotein (PAG) like proteins in red deer
Okuyama, MW; Melo de Sousa, Noelita ULg; Beckers, Jean-François ULg

Poster (2013, June 19)

The Pregnancy-Associated glycoproteins (PAGs) constitute an asparatic proteinase family and are divided into 2 groups; bovine PAG-1 (boPAG-1) group and boPAG-2 group. PAGs have been detected in many ... [more ▼]

The Pregnancy-Associated glycoproteins (PAGs) constitute an asparatic proteinase family and are divided into 2 groups; bovine PAG-1 (boPAG-1) group and boPAG-2 group. PAGs have been detected in many domestic ruminant species and their biochemical characteristics were reported. However, information in wild ruminant, especially in deer species is scarce. The aim of this study is to investigate the characteristics of placental protein extracted in red deer (cervus elaphus); placental proteins which belong to each type of PAG group were identified in purification steps. Fresh frozen placenta (fetal cotyledon (FC): 2,247 g, maternal cotyledon (MC): 2,255 g respectively) was used as following purification step; protein extraction, acid precipitation (pH 4.5), Ammonium sulfate precipitation (SA0-40%, SA40-80%) and anion exchange chromatography (DEAE-cellulose 0M, 0,02M, 0,04M, 0,08M, 0,16M and 0,32M NaCl). In every step, Immunoreactivity against anti-PAG antisera was checked by Radioimmunoassay with using anti-PAG-1 antiserum (AS706) and anti-boPAG-2 antiserum (AS438). After Ammonium sulfate precipitation, much higher concentrations were gained in SA40-80% step (AS706: 5.35 mg/FCkg and 6.73 mg/MCkg, AS438: 24.79 mg/FCkg and 84.6 mg/MCkg) than SA0-40% step (AS706: 0.64 mg/FCkg and 0.79 mg/MCkg, AS438: 1.3 mg/FCkg and 9.34 mg/MCkg) in both part of cotyledon. After DEAE chromatography, the highest concentrations of protein against AS438 were gained in 0.08M NaCl step (5.49 mg/FCkg and 17.8 mg/MCkg) and concentrations of protein against AS706 were almost same in 0.08M NaCl (1.01 mg/FCkg and 1.35 mg/MCkg) and 0.16M NaCl (0.93 mg/FCkg and 1.34 mg/MCkg). In many ruminants such as cattle, sheep and goat, PAGs are contained largely in fetal cotyledon and most amounts of PAGs are belonging to PAG-1 group. On the other hand, in red deer, much larger amount of PAGs was obtained from maternal cotyledon. And stronger immunoreactivity with anti-boPAG-2 antisera was detected than with anti-PAG-1 antisera. This characteristic is similar to the results which were reported in pig and dromedary. Therefore, these results suggest that PAGs in red deer have different characteristics from other ruminant species. [less ▲]

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See detailIntrafollicular LH administration in dairy heifers treated with a GnRH agonist
Mala, J; Beckers, Jean-François ULg; Melo de Sousa, Noelita ULg et al

in Veterinarni Medicina (2013), 58(2), 8186

The aim of this study was to evaluate the effect of intrafollicular treatment (IFT) with different doses of luteinising hormone. Experimental heifers were treated with a single deslorelin implant to ... [more ▼]

The aim of this study was to evaluate the effect of intrafollicular treatment (IFT) with different doses of luteinising hormone. Experimental heifers were treated with a single deslorelin implant to desensitise gonadotroph cells of the pituitary gland. Thereafter, follicular development was stimulated by exogenous FSH treatment. Intrafollicular treatment with 10, 5, 1 and 0.01 μg LH was performed on one single follicle while other follicles remained untreated. Human chorionic gonadotrophine (2000 UI) was administered intravenously as a control. Ovulation and development of the corpus luteum occurred after all intrafollicular treatments with 10 and 5 μg LH. After IFT using 1 μg of LH 75% animals (3/4) ovulated. The dose of 0.01 μg was not followed by any ovulation whereas control treatments with hCG were followed by an ovulation of the majority of follicles present in the ovaries. In conclusion, IFT with different doses of LH (greater than 0.01 μg) is capable of inducing ovulation. [less ▲]

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See detailProfiles of pregnancy-associated glycoprotein (PAG) concentrations during gestation in swamp buffalo
Van Hahn, N; Viet Linh, N; Melo de Sousa, Noelita ULg et al

in Proceedings of the 10th World Buffalo Congress and 7th Asian Buffalo Congress (2013)

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See detailPregnancy-Associated Glycoproteins (PAGs) in prolific (Boujaâd x D’man) and non- prolific (Boujaâd) Moroccan sheep by means of four radio-immunoassays
El Amiri, Bouchra; Melo de Sousa, Noelita ULg; El Abbadi, N et al

in Proceedings of the 23rd International Congress of Hungarian Association for Buiatrics (2013)

The aim of the present study was: i) to investigate the use of PAGs for pregnancy follow up in Boujaâd non prolific (n=8) vs. Boujaâd x D’man a prolific sheep (n=20), ii) to study whether the boPAG tracer ... [more ▼]

The aim of the present study was: i) to investigate the use of PAGs for pregnancy follow up in Boujaâd non prolific (n=8) vs. Boujaâd x D’man a prolific sheep (n=20), ii) to study whether the boPAG tracer (daily available at the laboratory of physiology of reproduction, Liège, could be used in RIA without affecting the results, iii) to prospect the possibility of transferring the technique to a Moroccan laboratory. The blood samples were weekly collected from the first week of gestation till the 4 week after lambing. The RIA1, RIA2, RIA3 and RIA4 were all based on ovPAG standard, while the tracer and antiserum changed according to the system. The RIA1, RIA3 and RIA4 were performed in Belgium while the RIA2 was carried out in Morocco. The results showed that the highest correlation was obtained between the RIA3 and RIA4 (R2 = 0.83) while the lowest was recorded between the RIA2 and RIA4 (R2 = 0.69). The lowest concentrations derived from the RIA2. The highest concentrations were recorded before lambing (279.29; 88.21; 268.75; 152.67 ng/ml respectively for RIAs 1, 2, 3 and 4). To conclude, the profiles of ovPAG showed that it is possible to flow-up with success using the four systems. In addition, the RIA1 using boPAG as tracer could be a good alternative to the homologous one and finally, it is possible to set up routinely the RIA technique for PAG detection in Moroccan laboratories. [less ▲]

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See detailPregnancy-associated glycoproteins in sheep: breeds, techniques and achievements
El Amiri, Bouchra; Melo de Sousa, Noelita ULg; Szenci, Otto et al

in Proceedings of the 23rd International Congress of Hungarian Association for Buiatrics (2013)

The aim of the present review is to summarize the achievements on pregnancy-associated glycoproteins in sheep during the last 20 years on terms of results obtained after purifications, molecular biology ... [more ▼]

The aim of the present review is to summarize the achievements on pregnancy-associated glycoproteins in sheep during the last 20 years on terms of results obtained after purifications, molecular biology, radio-immunoassays (RIA), enzyme linked immune sorbent assay (ELISA), double radial immune-diffusion (Ouchterlony) and Western blot techniques. The analysis of documented papers that concerned these attempts showed that the animal genetic material used was composed of variable breeds from north Africa, Europe and USA (the Aragonesa, Ouled Djellal, Laucaun drairy sheep, Berrichone, Texel x Norvegian crossed ewes, Awassi x Merino, Blackheaded Moutton sheep x Blackheaded German Mutton sheep (BGM), Rhoen sheep crosses of BGM with Dorper sheep, Assaf, Chura, Merino, Texel, Suffolk, Boujaâd Moroccan breed). The RIA represents the wildspread technique used (54%) while Oucherlony and Western blot had been limited in use (4% each). From 1990 to 2000 only 27% of the achievements being available today were realized. The main data, profiles and studies were performed from 2000 to now (63%). To conclude, this review allows summarizing and updating the knowledge about the PAG in sheep. [less ▲]

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See detailSerum albumin [Fragment] from Dama dama placenta - Accession number C0HJD2
Bériot, Mathilde ULg; Tchimbou Njanjo, Aline Flora; Barbato, Olimpia et al

E-print/Working paper (2013)

N-terminal microsequence obtained after purification and characterization of placental proteins in Dama dama (fallow deer). Proteins were submitted to SwissProt databank.

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See detailAlpha-2-HS-glycoprotein [Fragment] from Dama dama placenta - Accession number C0HJD1
Bériot, Mathilde ULg; Tchimbou Njanjo, Aline Flora; Barbato, Olimpia et al

E-print/Working paper (2013)

N-terminal microsequence obtained after purification and characterization of placental proteins in Dama dama (fallow deer). Proteins were submitted to SwissProt databank.

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See detailPregnancy-Associated Glycoprotein D-56 kDa [Fragment] from Dama dama placenta - Accession number C0HJD0
Bériot, Mathilde ULg; Tchimbou Njanjo, Aline Flora; Barbato, Olimpia et al

E-print/Working paper (2013)

N-terminal microsequence obtained after purification and characterization of placental proteins in Dama dama (fallow deer). Proteins were submitted to SwissProt databank.

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See detailPregnancy-Associated Glycoprotein C-39 kDa [Fragment] from Dama dama placenta - Accession number C0HJC9
Bériot, Mathilde ULg; Tchimbou Njanjo, Aline Flora; Barbato, Olimpia et al

E-print/Working paper (2013)

N-terminal microsequence obtained after purification and characterization of placental proteins in Dama dama (fallow deer). Proteins were submitted to SwissProt databank.

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See detailPregnancy-Associated Glycoprotein B-56 kDa [Fragment] from Dama dama placenta - Accession number C0HJC8
Bériot, Mathilde ULg; Tchimbou Njanjo, Aline Flora; Barbato, Olimpia et al

E-print/Working paper (2013)

N-terminal microsequence obtained after purification and characterization of placental proteins in Dama dama (fallow deer). Proteins were submitted to SwissProt databank.

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See detailPregnancy-Associated Glycoprotein A-62 kDa [Fragment] from Dama dama placenta - Accession number C0HJC7
Bériot, Mathilde ULg; Tchimbou Njanjo, Aline Flora; Barbato, Olimpia et al

E-print/Working paper (2013)

N-terminal microsequence obtained after purification and characterization of placental proteins in Dama dama (fallow deer). Proteins were submitted to SwissProt databank.

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See detailPregnancy-associated glycoprotein (PAG) pattern and pregnancy detection in Boer goats using an ELISA with different antisera
Shahin, M; Friedrich, M; Gauly, M et al

in Small Ruminant Research (2013), 113

Pregnancy-associated glycoproteins (PAGs) are macromolecules produced by placental tissue and released into the maternal circulation where they allow pregnancy diagnosis and follow-up. The present study ... [more ▼]

Pregnancy-associated glycoproteins (PAGs) are macromolecules produced by placental tissue and released into the maternal circulation where they allow pregnancy diagnosis and follow-up. The present study addresses the question to what extent plasma PAG determination may serve as a means of early pregnancy detection in goats in a similar way it is practiced in cows, and whether an ovine or bovine PAG-ELISA may be utilized to this end. Blood samples were collected from eight pregnant pluriparous Boer goat does twice weekly during the first seven seeks and the last four weeks of pregnancy and weekly in-between and during four weeks following parturition. Plasma PAG concentrations (mean±SEM) were determined using a competitive enzyme-linked immunosorbent assay. Assays were conducted with polyclonal antisera raised in rabbits against purified preparations of caprine (AS#706), ovine (AS#780) and bovine PAG (AS#726). In the assay systems purified bovine PAG served as standard and tracer and goat anti-rabbit IgG served as coating antibody. With the antibody raised against caprine PAG (AS#706) a steep increase to a climax of 69±9 ng/ml on day 56 of pregnancy was followed by a gradual decline to 16±3 ng/ml at parturition and 0.3±0.07 ng/ml four weeks postpartum. The results achieved with the antiovine PAG (AS#780) showed close similarity, a maximum of 92±14 ng/ml being reached at 56 days of pregnancy. With anti-bovine PAG (AS#726), the PAG level increased to a maximum of 3.1±0.2 ng/ml on day 105 of pregnancy and fluctuated around 3 ng/ml until the end of pregnancy. The difference between pregnant and non-pregnant does reached a significant level 21 days after conception, one week earlier than with caprine and ovine antisera. [less ▲]

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