References of "Beckers, Jean-François"
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See detailExperimental Neospora caninum infection modifies trophoblast cell populations and plasma pregnancy-associated glycoprotein 1 and 2 dynamics in pregnant dairy heifers.
Mur-Novales, R.; Serrano-Perez, B.; Garcia-Ispierto, I. et al

in Veterinary parasitology (2016), 216

Neospora caninum is an obligate intracellular protozoan that causes abortion in cattle worldwide. Plasma concentrations of pregnancy-associated glycoprotein 1 (PAG-1), produced by binucleate trophoblast ... [more ▼]

Neospora caninum is an obligate intracellular protozoan that causes abortion in cattle worldwide. Plasma concentrations of pregnancy-associated glycoprotein 1 (PAG-1), produced by binucleate trophoblast cells, are used for pregnancy diagnosis and as a marker of foetal-placental well-being, while PAG-2, produced by both mono- and binucleate trophoblast cells, may serve as an indicator of abortion risk. In prior work, natural N. caninum infection was found to modify plasma PAG-1 and PAG-2 patterns. The present study examines PAG-1 and PAG-2 dynamics and trophoblast cell populations following experimental infection with N. caninum. The study population was comprised of 17 N. caninum seronegative Holstein-Friesian heifers. On day 110 of gestation, 6 heifers were inoculated intravenously with 10(7) taquyzoites of N. canimum. Plasma samples for PAG-1 and PAG-2 determinations were collected on days 0, 7, 14, 21 and 42 post infection. During the study course, pregnancy was normally expressed in all controls while three infected heifers lost their foetuses. All heifers were euthanised on day 42 post infection and placentome samples from the 14 non-aborting heifers were collected to examine trophoblast cell populations. Plasma PAG-1 and PAG-2 concentrations in non-infected heifers increased until the day of euthanasia while non-aborting infected heifers showed a temporary fall in PAG-1 (P<0.004) and PAG-2 (P<0.002) concentrations from 7 to 14 days post infection (dpi). The two dams aborting at 14 and 21 dpi and a third dam with a mummified foetus upon euthanasia showed dramatic PAG-1 and PAG-2 reductions from 14 dpi to undetectable levels upon euthanasia. A stereological study of placentomes revealed significantly higher relative proportions of mono- (P=0.035) and binucleate (P=0.029) trophoblast cells at 42 dpi in non-infected heifers than infected non-aborting heifers. According to our findings, following experimental N. caninum infection on day 110 of gestation, non-aborting heifers showed a brief reversible drop in plasma PAG-1 and 2 concentrations two weeks later and reduced proportions of bi- and mono-nucleate trophoblast cells 42 days after infection. In aborting dams, dramatically reduced PAG levels were related to severe placental damage and a non-viable pregnancy. [less ▲]

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See detailIdentification of embryonic/fetal mortality in cows by semiquantitative detection of pregnancy-associated glycoproteins
Engelke, J; Knaack, H; Linden, M et al

in Livestock Science (2015), 178

Late embryonic and early fetal mortality (EM/FM) might occur following a pregnancy diagnosis, decreasing the accuracy of pregnancy diagnostics in dairy cattle. Therefore, the detection of EM/FM may be ... [more ▼]

Late embryonic and early fetal mortality (EM/FM) might occur following a pregnancy diagnosis, decreasing the accuracy of pregnancy diagnostics in dairy cattle. Therefore, the detection of EM/FM may be useful in dairy reproductive management. Comparatively low concentrations of pregnancy-associated glycoproteins (PAGs) have been previously associated with EM/FM. The aim of the present study was to test the ability of a semi- quantitative enzyme-linked immunosorbent assay for the detection of PAGs(PAG-ELISA) to either diagnose (EM/FM has already occurred) or to predict (EM/FM will occur) EM/FM. Three experiments were carried out as follows: (1) PAG-ELISA validation, (2) aretro-spective verification of 141 PAG results in cases with suspected EM/FM and the identification of cut-off values for EM/FM, and (3) a field experiment, testing the proposed PAG-ELISA cut-off values defined in Experiment 2 under farm conditions by comparing transrectal ultrasound (TRU) results with PAG-ELISA results. The PAG-ELISA validation (Experiment 1) demonstrated that anoptical density (OD) range of 0.4–2.8 reflected a linear relation to the PAG concentrations. The results from Experiment 2 indicated that more cows (85.7%) with PAG-OD values ranging from 0.40–0.79 showed EM/FM compared to cows (21.7%) with PAG-OD values ranging from 1.2 to 1.5. Acut-off valueof 2.2 (sensitivity [SEN]=87.6%; specificity [SPE]=72.8%) was determined using ROC-curve analysis for prediction of EM/FM and a cut-off value of 1.2 for diagnosis of EM/FM (SEN=50%; SPE=94%). However, in the field experiment, EM/FM was best diagnosed using a cut-off value of 1.5 (SEN=26.7% and SPE=96.5%). Hence, the high SEN detected in Experiment 2 could not be confirmed in the field experiment indicating that a singular PAG value is not a convenient tool to diagnose EM/FM. Though, the combined use of PAG-ELISA and TRU showed remarkable results (SEN=84.2%; SPE=98.1%) by revealing a technique for diagnosis of previously occurred EM/FM and for prediction of subsequent occurring EM/FM. [less ▲]

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See detailComparison of a commercial bovine pregnancy-associated glycoprotein ELISA test and a pregnancy-associated glycoprotein radiomimmunoassay test for early pregnancy diagnosis in dairy cattle.
Karen, Aly; Melo de Sousa, Noelita ULg; Beckers, Jean-François ULg et al

in Animal reproduction science (2015), 159

The present study aimed to compare the accuracy of a commercial PAG-ELISA test (Bovine Preg Test 29) and bovine pregnancy-associated glycoprotein radioimmunoassay (PAG-RIA) for diagnosing pregnancy at Day ... [more ▼]

The present study aimed to compare the accuracy of a commercial PAG-ELISA test (Bovine Preg Test 29) and bovine pregnancy-associated glycoprotein radioimmunoassay (PAG-RIA) for diagnosing pregnancy at Day 28 after insemination in dairy cows. Transrectal ultrasonography (TRUS) was performed in 100 Holstein-Friesian cows at Day 28 after artificial insemination (AI; Day 0) to diagnose pregnancy. After TRUS examination, blood sample was collected from the coccygeal vessels of each cow to measure the concentrations of bPAGs by PAG-RIA test and Bovine Preg Test 29. Milk samples were collected at Days 0, 21 and 28 for measurement of progesterone (P4) by ELISA test. The cows were re-examined by TRUS at Day 42 to confirm the pregnancy diagnoses. The actual gold standard was based on TRUS outcomes at Day 28 that agreed with the outcomes of PAG-RIA test or PAG-ELISA test. If the outcomes of TRUS at Day 28 and PAG-RIA test and PAG-ELISA test did not agree, the gold standard was based on the outcome of TRUS at Day 42. Out of 100 inseminated cows, 41 were confirmed pregnant at Day 28 after AI. Based on the actual gold standard, the sensitivity of TRUS, PAG-ELISA and PAG-RIA tests for diagnosing pregnant cows at Day 28 were 92.7%, 90.2% and 100%, while the specificity of the three tests for diagnosing non-pregnant cows were 91.5%, 98.3% and 94.4%, respectively. The overall accuracy of the three tests were 92%, 95% and 97%, respectively. The degree of agreement (Kappa+/-S.E.) between PAG-RIA and PAG-ELISA test was 0.90 +/-0.04. The degrees of agreement between PAG-RIA and PAG-ELISA and TRUS at Day 28 were 0.80+/-0.05 and 0.76+/-0.06, respectively. In conclusion, the commercial PAG-ELISA test is a highly accurate method for diagnosing early pregnancy in dairy cows on Day 28 after AI and may be used as an alternative method to the TRUS and the PAG-RIA test. [less ▲]

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See detailMilk prolactin response and quarter milk yield after experimental infection with coagulase-negative staphylococci in dairy heifers.
Piccart, K.; Piepers, S.; Verbeke, J. et al

in Journal of dairy science (2015), 98(7), 4593-4600

Coagulase-negative staphylococci (CNS) are the most common bacteria involved in subclinical mastitis in dairy cows. Remarkably, CNS-infected dairy heifers produce more milk than uninfected heifers ... [more ▼]

Coagulase-negative staphylococci (CNS) are the most common bacteria involved in subclinical mastitis in dairy cows. Remarkably, CNS-infected dairy heifers produce more milk than uninfected heifers. Because the lactation hormone prolactin (PRL) is also involved in mammary gland immunity, we investigated the milk PRL response and the mammary quarter milk yield following experimental CNS challenge. Eight healthy Holstein-Friesian heifers in mid-lactation were experimentally infected using a split-udder design with 3 different CNS strains: one Staphylococcus fleurettii (from sawdust bedding) and 2 Staphylococcus chromogenes strains (one isolate from a teat apex, the other isolate from a chronic intramammary infection). Three mammary quarters per heifer were simultaneously inoculated with 1.0x10(6) cfu, whereas the remaining mammary quarter was infused with sterile phosphate-buffered saline, serving as a control. An existing radioimmunoassay was modified, validated, and used to measure PRL frozen-thawed milk at various time points until 78h after challenge. The mean milk PRL level tended to be higher in the CNS-challenged mammary quarters compared with the control mammary quarters (7.56 and 6.85ng/mL, respectively). The increase in PRL over time was significantly greater in the CNS-challenged mammary quarters than in the control mammary quarters. However, no difference was found in the PRL response when comparing each individual CNS strain with the control mammary quarters. The mean mammary quarter milk yield tended to be lower in the CNS-infected mammary quarters than in the control mammary quarters (1.73 and 1.98kg per milking, respectively). The greatest milk loss occurred in the mammary quarters challenged with the intramammary strain of S. chromogenes. Future observational studies are needed to elucidate the relation between PRL, the milk yield, and the inflammatory condition, or infection status, of the mammary gland. [less ▲]

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See detailEffects of crossbreeding on endocrine patterns determined in pregnant beef/dairy cows naturally infected with Neospora caninum.
Garcia-Ispierto, I.; Serrano-Perez, B.; Almeria, S. et al

in Theriogenology (2015), 83(4), 491-496

Neospora caninum is an intracellular coccidian parasite causing abortion worldwide in dairy cattle. Studies have shown that N caninum infection modifies endocrine patterns and that beef cows or cows with ... [more ▼]

Neospora caninum is an intracellular coccidian parasite causing abortion worldwide in dairy cattle. Studies have shown that N caninum infection modifies endocrine patterns and that beef cows or cows with a crossbreed pregnancy, especially for a greater maternal-paternal genetic distance, show a reduced risk of abortion when naturally infected compared with pure-breed Holstein Friesian (HF) pregnancies. This study examined the effects of crossbreeding on plasma progesterone (P4), pregnancy-associated glycoprotein (PAG)-1 and -2, and prolactin patterns produced during gestation in N caninum-infected beef/dairy cows. We analyzed 74 pregnancies in nonaborting cows carrying a single fetus established in 26 lactating HF cows bred with Holstein bulls (H-H), 13 HF cows bred with Limousin bulls (H-L), 12 HF cows used as recipients of transferred in vivo-produced frozen-thawed Rubia Gallega (beef breed; RG) embryos (H-RG-ET), and 23 RG suckling cows bred with RG bulls (RG-RG). Of the cows, 29 (39%) were seropositive for N caninum. Blood samples for hormone and placental protein determinations were collected on Days 30, 60, 90, 120, 150, and 180 of gestation. Procedures of repeated measures analysis of variance throughout gestation revealed higher PAG-1 levels in the H-L and H-RG-ET groups compared with H-H and RG-RG. Higher prolactin levels were detected in RG-RG than in the remaining groups. N caninum seropositivity and fetal genotype had a significant effect on PAG-2 concentrations, such that highest levels of PAG-2 occurred in RG-RG seropositive cows and lowest in H-H seropositive cows, whereas Neospora-seropositive cows showed lower P4 concentrations than their seronegative partners. In conclusion, chronic N caninum infection modifies endocrine patterns of PAG-2 and P4 during pregnancy, whereas PAG-1 and prolactin concentrations are affected by breed and fetal genotype, irrespective of Neospora infection status. [less ▲]

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See detailAccuracy of diagnosing double corpora lutea and twin pregnancy by measuring serum progesterone and bovine pregnancy-associated glycoprotein 1 in the first trimester of gestation in dairy cows
Szelenyi, Zoltan; Répási, Attila; Melo de Sousa, Noelita ULg et al

in Theriogenology (2015), 84(1), 76-81

Progesterone (P4) and bovine pregnancy-associated glycoprotein 1 (bPAG-1) concentrations during gestation are dependent on the number of CL and fetuses, respectively. The objective of this present study ... [more ▼]

Progesterone (P4) and bovine pregnancy-associated glycoprotein 1 (bPAG-1) concentrations during gestation are dependent on the number of CL and fetuses, respectively. The objective of this present study was to measure and evaluate the usefulness of measuring the P4 and bPAG-1 concentrations in cases of single versus twin pregnancies and one versus two CL at the first 4 months of gestation. We hypothesized that both the number of the CL and the number of fetus might have an effect on P4 and pregnancy protein concentrations, and we can set up clinically useful threshold levels to predict twin gestations. Eighty-four Holstein-Friesian dairy cows were enrolled in this prospective observational clinical trial. Blood was collected at time point 1: between Days 29 and 42, time point 2: between Days 57 and 70, time point 3: between Days 85 and 98, and time point 4: between Days 113 and 126 of gestation, and bPAG-1 and P4 concentrations were measured. Binary logistic regression analyzing serum P4 concentrations differed at time point 2 compared with baseline level, but the area under the curve (AUC) had low sensitivity. The bPAG-1 concentrations were statistically different at each time point of gestation. The AUC cutoff values of serum bPAG-1 concentrations were sufficiently sensitive to differentiate between twin gestations from singleton ones. At time points 3 (cutoff value of 3.4 ng/mL) and 4 (cutoff value of 56.5 ng/mL), statistically significant differences with low sensitivity, high specificity, and a high AUC were found. On the basis of these results, the diagnosis of twin pregnancy using pregnancy protein measurements is clinically insufficient before Day 85 of gestation; however, the ability to confirm the early twin pregnancy diagnosis with bPAG-1 measurements appears to be promising. To achieve high sensitivity, further studies are required. [less ▲]

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See detailPregnancy-associated glycoprotein (PAG) concentration in plasma and milk samples for early pregnancy diagnosis in Lacaune dairy sheep
El Amiri, Bouchra; Melo de Sousa, Noelita ULg; Alvarez-Oxiley, Andrea et al

in Research in Veterinary Science (2015), 99

In the present study, four RIA systems (RIA-1 to -4) based on two antisera raised against ovine pregnancyassociated glycoproteins (ovPAGs), combined with an ovine or a bovine PAG tracer were used to ... [more ▼]

In the present study, four RIA systems (RIA-1 to -4) based on two antisera raised against ovine pregnancyassociated glycoproteins (ovPAGs), combined with an ovine or a bovine PAG tracer were used to measure PAG concentrations in plasma and milk samples of dairy ewes. Blood and milk samples were collected on different days of gestation: 0, 18, 20, 22, 25, 28, 32, 42, and 49. From day 20 onward, the PAG in plasma could be detected in all pregnant ewes using the four RIA systems. By using milk, except for RIA-1, the other systems showed a sensitivity of 100% from day 28 of gestation onward. In plasma, PAG concentrations were higher in multiple than in single pregnancies, while no clear relationship was observed in milk. In conclusion, milk is a good alternative to plasma for early pregnancy diagnosis in sheep from day 28 to day 42. [less ▲]

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See detailEffect of bovine colostrum on growth and survival in Red kids during the first year of life.
Abdou, Harouna ULg; Marichatou, Hamani; Beckers, Jean-François ULg et al

Poster (2014, October 17)

The aim of this work was to measure the effect of supplementation with bovine colostrum among newborn Red goats breastfed in breeding conditions for semi-intensive type of Niger. The study was conducted ... [more ▼]

The aim of this work was to measure the effect of supplementation with bovine colostrum among newborn Red goats breastfed in breeding conditions for semi-intensive type of Niger. The study was conducted in Secondary Goat Breeding Center of Maradi (SGBCM). Forty newborn kids, regardless of sexe, were divided randomly into two groups: a "T-Control " group receiving only breast milk per feeding (n = 20) and a "C-Colostrum" group having the same diet but supplemented with bovine colostrum thawed (50 ml the first day of life, then 25 ml in 2 meals/ day between the ages of 2 and 15d, n = 20). The results indicate that supplementation with bovine colostrum in the first 15 days of life, increases the growth of kids until weaning (P<0.001), modifie some barymetric changes some settings improves health status and reduces the mortality rate. To our knowledge, in the studied environment, this work is original and seems to bring new knowledges likely to have practical applications in areas with farming conditions quite difficult. [less ▲]

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See detailChemical composition of colostrum from Azawak cow in Niger compared with meta-analytical data
Abdou, Harouna; Marichatou, Hamani; Beckers, Jean-François ULg et al

in Animal Genetic Resources Information = Bulletin d'Information sur les Ressources Génétiques Animales = Boletin de Informacion sobre Recursos Geneticos Animales (2014), 55

This study aimed at comparing data obtained from Azawak zebu colostrum with literature data. The comparison was performed by a meta-analytical approach. Colostrum samples were hand-collected after 5 h ... [more ▼]

This study aimed at comparing data obtained from Azawak zebu colostrum with literature data. The comparison was performed by a meta-analytical approach. Colostrum samples were hand-collected after 5 h from 7 Azawak cows at calving between August 27, to September 10, 2009 in the Sahel. For data from literature, twenty one (21) references were identified in the following analytical databases: PubMed, Science Direct, Google scholar, Collection from University of Liege. The references were selected according to the following two criteria: i) only studies reported on bovine colostrum were used irrespective of breeds, and ii) among the selected studies, those not providing complete information to allow meta-analytical calculation were excluded. Samples were analyzed for immunoglobulins (IgG, IgM, IgA), lactoferrin, and chemical composition (dry mater, protein, fat, lactose, ash, Ca, P, NA, K, Mg). The mean levels of IgG, IgM, dry matter, protein and fat for Azawak cows were lower (P ˂ 0.001) than those obtained in other breeds; however colostrum from the Azawak was higher in IgA but the difference was not significant. For lactose and ash, mean values for Azawak cows were higher (P ˂ 0.001) than those from the literature. Contents of Ca, P, Na and Mg in Azawak bovine colostrum were significantly higher (P ˂ 0.001) than the mean levels in form the literature data. In conclusion, the colostrum from Azawak cows appears to be lower in most immunoglobulins, in fat and in protein than the values reported in the literature, but higher in lactose and minerals. This could be an adaptation to sahelian contraints. [less ▲]

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See detailEffect of bovine colostrum intake on growth, reproductive parameters and survival in red kids
Harouna, Abdou; Marichatou, Hamani; Beckers, Jean-François ULg et al

in Journal of Animal Physiology & Animal Nutrition (2014), 98(5), 845-852

The aim of this study is to evaluate the efficacy of frozen Azawak colostrum supplementation on body weight (BW), average daily gain (ADG), reproductive parameters (mean age at first parturition ... [more ▼]

The aim of this study is to evaluate the efficacy of frozen Azawak colostrum supplementation on body weight (BW), average daily gain (ADG), reproductive parameters (mean age at first parturition, fertility, fecundity, prolificacy) and mortality rate among red kids. The study was conducted at the goat farm secondary centre of Maradi in Niger from September 2010 to September 2011. The control animals (n = 20) were left with their mother, while the treatment animals (n = 20) received in addition 50 ml/animal/day of bovine colostrum at birth and 15 ml/animal/day from d2 to d15. Weight was measured weekly from birth to d365. Mortalities were also recorded over the same period. For reproductive parameters, observations began at weaning (d197). Growth rate was higher (p < 0.001) in supplemented animal, and the treatment effects on ADG were observed up to 150 day after the end of supplementation. A similar long-lasting trend was also observed in relation to the mortality rate (25% for ColG vs. 55% for ConG; p = 0.05). The age at first kidding tended to be lower in the treated group (13.8 0.7 vs. 14.1 0.8 month; p < 0.1). In conclusion, mild bovine colostrum supplementation induces a long-lasting positive impact on growth rate and to a lower extent on reproduction parameters and survival rate. [less ▲]

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See detailRelationship of progesterone, bovine pregnancy-associated glycoprotein-1 and nitric oxide with late embryonic and early fetal mortalities in dairy cows
Karen, A; Bajcsy, AC; Minoia, R et al

in Journal of Reproduction and Developmen (2014), 60(2), 162-167

The aim of the present study was to determine the relationship of progesterone (P4), bovine pregnancy-associated glycoprotein-1 (bPAG-1) and nitric oxide (NO) levels with late embryonic (LEM; day 28 to ... [more ▼]

The aim of the present study was to determine the relationship of progesterone (P4), bovine pregnancy-associated glycoprotein-1 (bPAG-1) and nitric oxide (NO) levels with late embryonic (LEM; day 28 to day 42) and early fetal mortalities (EFM; > day 42 to day 56) in dairy cows. Transrectal ultrasonography (6-8 MHz) was performed in 100 Holstein-Friesian cows at days 28, 42 and 56 after artificial insemination (AI; day 0) to diagnose pregnancy and to monitor the fate of the embryo. After ultrasound scanning of each cow, a milk sample was collected for assessment of P4 by an ELISA test and a blood sample was collected for assessment of bPAG-1, by using a double-antibody radioimmunoassay, and serum NO metabolites (nitrate + nitrite). Based on ultrasonographic examinations and bPAG-1-RIA, 41 of 100 inseminated cows were confirmed pregnant at day 28 after AI. Nine cows suffered of LEM, and 6 cows suffered of EFM and the overall pregnancy loss rate was 36.6% (15/41) between days 28 and 56 of pregnancy. By logistic regression analysis, there were no significant relationships between the level of P4 and bPAG-1 at day 28 after AI and the occurrence of LEM and EFM. Also, there were no significant relationships between the levels of P4 and bPAG-1 at day 42 and the occurrence of EFM. On the other hand, a significant relationship (P<0.05) was found between NO level at day 28 and the occurrence of LEM. In conclusion, measurement of the serum NO concentration at day 28 of pregnancy might help to predict the outcome of pregnancy by day 42 in dairy cows but further studies are needed to confirm this. [less ▲]

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See detailGamma interferon production and plasma concentrations of pregnancy-associated glycoproteins 1 and 2 in gestating dairy cows naturally infected with Neospora caninum.
Serrano-Perez, B; Garcia-Ispierto, I; Melo de Sousa, Noelita ULg et al

in Reproduction in Domestic Animals (2014), 49(2), 275-280

Gamma interferon (IFN-γ) production and cross-breed pregnancy have been attributed a role in protecting dairy cows infected with Neospora caninum against abortion. Plasma levels of pregnancy-associated ... [more ▼]

Gamma interferon (IFN-γ) production and cross-breed pregnancy have been attributed a role in protecting dairy cows infected with Neospora caninum against abortion. Plasma levels of pregnancy-associated glycoproteins-1 (PAG-1) are a marker of placental/foetal well-being and of PAG-2 is an abortion risk indicator in chronically N. caninum-infected animals. The present study examines, in cross-breed pregnancies, interactions between IFN-γ production and levels of PAG-1 and PAG-2 in non-aborting naturally Neospora-infected dairy cows. Data were obtained from 60 pregnant Holstein-Friesian cows: 44 Neospora-seropositive and 16 Neospora-seronegative; 12 became pregnant using Holstein-Friesian semen and 48 using Limousin semen. Blood samples were collected on Days 40, 90, 120, 150, 180 and 210 of gestation. Gamma interferon was only detected in the plasma of nine of the 44 Neospora-seropositive cows, all of them became pregnant using Limousin semen. Through GLM procedures, in cows inseminated with Limousin semen and Neospora-seropositive cows showing no IFN-γ production, PAG-1 concentrations were high and increased throughout gestation compared to the levels detected in cows inseminated with Holstein-Friesian semen and Neospora-seropositive cows producing IFN-γ, respectively. In Neospora-seronegative cows and in Neospora-seropositive cows showing no IFN-γ production, significantly increased PAG-2 concentrations were observed on gestation Day 120. Our findings indicate that IFN-γ production correlates negatively and the production of antibodies against N. caninum is uncorrelated with plasma PAG concentrations during gestation in Neospora-infected dairy cows. Accordingly, IFN-γ production could be linked to the transplacental migration of tachyzoites, which may cause a reduction in PAG levels. [less ▲]

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See detailInvestigation of pregnancy-associated glycoproteins (PAGs) by means of an enzymoimunoassay (ELISA) sandwich kit for pregnancy monitoring in sheep
El Amiri, Bouchra; Delahaut, Philippe; Colemans, Yves et al

in Chentouf, M; López-Francos, A; Bengoumi, M (Eds.) et al Options méditerranéennes : Series A (2014)

The aim of the present study was to investigate the use of a PAGs ELISA-Sandwich kit (Ret. code E.G.7. CER. Marloie, Belgium) for both early pregnancy diagnosis (in Sardi sheep) and the pregnancy follow ... [more ▼]

The aim of the present study was to investigate the use of a PAGs ELISA-Sandwich kit (Ret. code E.G.7. CER. Marloie, Belgium) for both early pregnancy diagnosis (in Sardi sheep) and the pregnancy follow up (in Boujaâd and Boujaâd x D'man sheep). ln Sardi breed, plasma samples were obtained from pregnant ewes (n = 17) from day 18 to 30 of gestation at 2 days interval. ln Boujaâd (n = 8) and Boujaâd x D'man (n = 20) the blood samples were weekly collected from the first week of gestation till the 4th week after lambing. The PAG concentrations were determined by a sandwich-ELISA based on purified bovine PAG (boPAG-67 kDa) as a standard, the antiserum raised against caprine PAG (caPAG-55+62 kDa) as a capture antibody (1/40000) and antiserum raised against purified PAG from buffalo (AS 859) as detection antibody (1/32 000). The Avidin-HRP and TMB were used to reveal reactions. Ewes were assumed to be pregnant when PAG concentrations were higher than 0.8 ng/ml. Results showed that in Sardi sheep it is possible to detect ail ewes as pregnant as early as on 24 days of gestation. ln Boujaâd and Boujaâd x D'man the PAG concentrations reached the higher values just before lambing. This value is higher in ewes carrying more than one lamb than in those carrying a single lamb. To conclude, the present study shows that the ELISA kit used for early pregnancy diagnosis in sheep as weil as for pregnancy fellow up could be a good alternative to the radioimmunoassay RIA in countries where this last technique is hard to set up. [less ▲]

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See detailIn vitro embryo production in goats: slaughterhouse and laparoscopic ovum pick up 2 (LOPU) derived oocytes have different kinetics and requirements regarding maturation 3 media
Souza-Fabjan, JM; Locatelli, Y; Duffard, N et al

in Theriogenology (2014), 81

A total of 3427 goat oocytes were used in this study in order to identify possible 24 differences during in vitro embryo production from slaughterhouse or laparoscopic ovum 25 pick up (LOPU) oocytes. In ... [more ▼]

A total of 3427 goat oocytes were used in this study in order to identify possible 24 differences during in vitro embryo production from slaughterhouse or laparoscopic ovum 25 pick up (LOPU) oocytes. In Experiment 1, one complex, one semi defined and one simplified 26 in vitro maturation (IVM) media were compared using slaughterhouse oocytes. In 27 Experiment 2, we checked the effect of oocyte origin (slaughterhouse or LOPU) on the 28 kinetics of maturation (18 vs. 22 vs. 26 h) when submitted to semi defined or simplified 29 media. In Experiment 3, we determined the differences in embryo development between 30 slaughterhouse and LOPU oocytes when submitted to both media and then to in vitro 31 fertilization (IVF) or parthenogenetic activation (PA). Embryos from all groups were vitrified 32 and their viability evaluated in vitro after thawing. In Experiment 1, no difference (P>0.05) 33 was detected among treatments for maturation rate (MII; 88% on average), cleavage (72%), 34 blastocyst from the initial number of cumulus oocyte complexes (COC; 46%) or from the 35 cleaved ones (63%), hatching rate (69%) and the total number of blastomeres (187). In 36 Experiment 2, there was no difference of MII rate between slaughterhouse oocytes cultured 37 for 18 or 22 h, whereas the MII rate increased significantly (P<0.05) between 18 and 22 h for 38 LOPU oocytes in the simplified medium. Moreover, slaughterhouse oocytes cultured in 39 simplified medium matured significantly faster than LOPU oocytes at 18 and 22 h (P<0.05). 40 In Experiment 3, cleavage rate was significantly greater (P<0.001) in all four groups of 41 embryos produced by PA than IVF. Interestingly, PA reached similar rates for slaughterhouse 42 oocytes cultured in both media, but improved (P<0.05) cleavage rate of LOPU oocytes. 43 Slaughterhouse oocytes had acceptable cleavage rate after IVF (~67%), whereas LOPU 44 oocytes displayed a lower one (~38%), in contrast to cleavage after PA. The percentage of 45 blastocysts in relation to cleaved embryos was not affected by the origin of the oocytes 46 (P>0.05). Therefore, slaughterhouse oocytes developed a greater proportion of blastocysts than LOPU ones, expressed as the percentage of total COC entering to IVM. Vitrified-thawed 48 blastocysts presented similar survival and hatching rates between the oocyte origin, media or 49 method of activation. In conclusion, slaughterhouse and LOPU derived oocytes may have 50 different IVM kinetics and require different IVM-IVF conditions. Although the IVM and IVF 51 systems still need improvements in order to enhance embryo yield, the in vitro development 52 (IVD) step is able to generate good quality embryos from LOPU derived oocytes. [less ▲]

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See detailIdentification of pregnancy-associated glycoproteins and alpha-fetoprotein in fallow deer (Dama dama) placenta
Bériot, Mathilde ULg; Tchimbou Njanjo, Aline Flora ULg; Barbato, Olimpia et al

in Acta Veterinaria Scandinavica (2014), 56(4), 1-11

Background: This paper describes the isolation and characterization of pregnancy-associated glycoproteins (PAG) from fetal cotyledonary tissue (FCT) and maternal caruncular tissue (MCT) collected from ... [more ▼]

Background: This paper describes the isolation and characterization of pregnancy-associated glycoproteins (PAG) from fetal cotyledonary tissue (FCT) and maternal caruncular tissue (MCT) collected from fallow deer (Dama dama) pregnant females. Proteins issued from FCT and MCT were submitted to affinity chromatographies by using Vicia villosa agarose (VVA) or anti-bovine PAG-2 (R#438) coupled to Sepharose 4B gel. Finally, they were characterized by SDSPAGE and N-terminal microsequencing. Results: Four distinct fallow deer PAG (fdPAG) sequences were identified and submitted to Swiss-Prot database. Comparison of fdPAG with PAG sequences identified in other ruminant species exhibited 64 to 83% identity. Additionally, alpha-fetoprotein was identified in fetal and maternal tissues. Conclusion: Our results demonstrate the efficacy of VVA and bovine PAG-2 affinity chromatographies for the isolation of PAG molecules expressed in deer placenta. This is the first report giving four specific amino acid sequences of PAG isolated from feto-maternal junction (FCT and MCT) in the Cervidae family. [less ▲]

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See detailEffect of bovine colostrum administration on plasma protein profile, growth, and survival in Red kid.
Abdou, Harouna ULg; Marichatou, Hamani; Beckers, Jean-François ULg et al

in Small Ruminant Research (2013), 117

This study evaluated the effect of colostrum from Azawak cows on plasma protein profile, 24 growth, and survival in Red kids from Niger. Forty (40) newborn kids were allocated to one 25 of two treatment ... [more ▼]

This study evaluated the effect of colostrum from Azawak cows on plasma protein profile, 24 growth, and survival in Red kids from Niger. Forty (40) newborn kids were allocated to one 25 of two treatment-groups: control (free access to water and the mother) and colostrum (free 26 access to water and the mother, but with additional 50 mL of colostrum/animal/day of birth 27 and 25 mL/animal/day from the 2nd to 15th day of age). Blood samples were collected into 28 EDTA vacutainer tubes by jugular puncture at 10 and 30 days of age. Total protein was 29 quantified by the Biuret method. The agarose gel electrophoresis was used to determine the 30 serum levels of albumin, -globulin, β1-globulin, β2-globulin, γ-globulin and the 31 albumin/globulin ratio. The animals from the colostrum group showed higher body weight 32 and average daily gain when compared to the control group (P < 0.001). The average 33 concentration in protein at the both sampling times reached higher values in the colostrum 34 than in the control group. At D10, the colostrum group tended (P < 0.07) to show higher 35 levels of -globulin and had higher values (P < 0.05) for β1-globulin. At D30, total protein 36 and β2-globulins were higher in colostrum group. Colostrum from Azawak cows seems to 37 have positive effects on some plasma proteins levels and on growth rate in Red kids. [less ▲]

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See detailVitrification of immature equine oocytes
Gatez, Carine ULg; Ectors, Fabien ULg; Farnir, Frédéric ULg et al

Poster (2013, October 11)

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See detailCharacteristics of Pregnancy-Associated glycoprotein (PAG) like proteins in red deer
Okuyama, MW; Melo de Sousa, Noelita ULg; Beckers, Jean-François ULg

Poster (2013, June 19)

The Pregnancy-Associated glycoproteins (PAGs) constitute an asparatic proteinase family and are divided into 2 groups; bovine PAG-1 (boPAG-1) group and boPAG-2 group. PAGs have been detected in many ... [more ▼]

The Pregnancy-Associated glycoproteins (PAGs) constitute an asparatic proteinase family and are divided into 2 groups; bovine PAG-1 (boPAG-1) group and boPAG-2 group. PAGs have been detected in many domestic ruminant species and their biochemical characteristics were reported. However, information in wild ruminant, especially in deer species is scarce. The aim of this study is to investigate the characteristics of placental protein extracted in red deer (cervus elaphus); placental proteins which belong to each type of PAG group were identified in purification steps. Fresh frozen placenta (fetal cotyledon (FC): 2,247 g, maternal cotyledon (MC): 2,255 g respectively) was used as following purification step; protein extraction, acid precipitation (pH 4.5), Ammonium sulfate precipitation (SA0-40%, SA40-80%) and anion exchange chromatography (DEAE-cellulose 0M, 0,02M, 0,04M, 0,08M, 0,16M and 0,32M NaCl). In every step, Immunoreactivity against anti-PAG antisera was checked by Radioimmunoassay with using anti-PAG-1 antiserum (AS706) and anti-boPAG-2 antiserum (AS438). After Ammonium sulfate precipitation, much higher concentrations were gained in SA40-80% step (AS706: 5.35 mg/FCkg and 6.73 mg/MCkg, AS438: 24.79 mg/FCkg and 84.6 mg/MCkg) than SA0-40% step (AS706: 0.64 mg/FCkg and 0.79 mg/MCkg, AS438: 1.3 mg/FCkg and 9.34 mg/MCkg) in both part of cotyledon. After DEAE chromatography, the highest concentrations of protein against AS438 were gained in 0.08M NaCl step (5.49 mg/FCkg and 17.8 mg/MCkg) and concentrations of protein against AS706 were almost same in 0.08M NaCl (1.01 mg/FCkg and 1.35 mg/MCkg) and 0.16M NaCl (0.93 mg/FCkg and 1.34 mg/MCkg). In many ruminants such as cattle, sheep and goat, PAGs are contained largely in fetal cotyledon and most amounts of PAGs are belonging to PAG-1 group. On the other hand, in red deer, much larger amount of PAGs was obtained from maternal cotyledon. And stronger immunoreactivity with anti-boPAG-2 antisera was detected than with anti-PAG-1 antisera. This characteristic is similar to the results which were reported in pig and dromedary. Therefore, these results suggest that PAGs in red deer have different characteristics from other ruminant species. [less ▲]

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See detailThe prenatal development in swamp buffalo
Van Hanh, N; Melo de Sousa, Noelita ULg; Beckers, Jean-François ULg et al

in Iranian Journal of Veterinary Research (2013), 14(4), 313-319

There are morphological and reproductive physiological differences between swamp buffalo (Bubalus carabanensis) and river buffalo (Bubalus bubalis). The development of fetus weight and fetus biometry was ... [more ▼]

There are morphological and reproductive physiological differences between swamp buffalo (Bubalus carabanensis) and river buffalo (Bubalus bubalis). The development of fetus weight and fetus biometry was reported in river buffalo and other animals but not in swamp buffalo. The aim of this study was to describe the inherent variability in fetus related measurements during swamp buffalo pregnancy. The data is based on measurements of 267 fetuses and 5 new born claves from swamp buffalo. The results show that a significant linear correlation exists between estimated age of fetuses and parameters of fetus sizes. There were correlations between crown-rump length (CRL) and other fetal parameters, as well as between fetus weight and its parameters. In conclusion, our data indicated that the feasibility and value of fetal measures in swamp buffaloes being used for the evaluation of fetal development. [less ▲]

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