  Sex and PRNP genotype determination in preimplantation caprine embryos; ; et al in Reproduction in Domestic Animals (2011), 46(4), 656-663 The objective of this study was to test the accuracy of genotype diagnosis after whole amplification of DNA extracted from biopsies obtained by trimming goat embryos and to evaluate the viability of ... [more ▼] The objective of this study was to test the accuracy of genotype diagnosis after whole amplification of DNA extracted from biopsies obtained by trimming goat embryos and to evaluate the viability of biopsied embryos after vitrification/warming and transfer. Whole genome amplification (WGA) was performed using Multiple Displacement Amplification (MDA). Sex and prion protein (PRNP) genotypes were determined. Sex diagnosis was carried out by PCR amplification of ZFX/ZFY and Y chromosome-specific sequences. Prion protein genotype determination was performed on codons 142, 154, 211, 222 and 240. Embryos were collected at day 7 after oestrus and biopsied either immediately after collection (blastocysts and expanded blastocysts) or after 24 h of in vitro culture (compacted morulae). Biopsied embryos were frozen by vitrification. Vitrified whole embryos were kept as control. DNA of biopsies was extracted and amplified using MDA. Sex diagnosis was efficient for 97.4% of biopsies and PRNP genotyping was determined in 78.7% of biopsies. After embryo transfer, no significant difference was observed in kidding rate between biopsied and vitrified control embryos, whereas embryo survival rate was different between biopsied and whole vitrified embryos (p = 0.032). At birth, 100% of diagnosed sex and 98.2% of predetermined codons were correct. Offspring PRNP profiles were in agreement with parental genotype. Whole genome amplification with MDA kit coupled with sex diagnosis and PRNP genotype predetermination are very accurate techniques to genotype goat embryos before transfer. These novel results allow us to plan selection of scrapie-resistant genotypes and kid sex before transfer of cryopreserved embryo. [less ▲] Detailed reference viewed: 34 (3 ULg)Embryo transfer as a tool for experimental reproduction of ovine herds.; ; et al in Ciência Animal Brasileira (2009), Suppl 1 Detailed reference viewed: 8 (3 ULg)Comparison of pregnancy-associated glycoprotein and oestrone sulphate concentrations in recipient goats of in vitro produced embryos cultured in synthetic oviduct fluid (fresh and vitrified) or co-cultured with goat epithelial oviduct cells (vitrified); ; et al in Reproduction in Domestic Animals (2008), 43(4), 55 Detailed reference viewed: 10 (1 ULg)Lack of risk of transmission of caprine arthritis-encephalitis virus (CAEV) after an appropriate embryo transfer procedure; ; Bouzar, Amel  et alin Theriogenology (2008) Detailed reference viewed: 7 (0 ULg)Improved vitrification method allowing direct transfer of goat embryos; ; et al in Theriogenology (2006), 66(4), 1004-1011 The aim of this study was to design a vitrification method suited to field embryo transfer experiments in goat. In a first experiment, a standard vitrification protocol, previously designed for sheep ... [more ▼] The aim of this study was to design a vitrification method suited to field embryo transfer experiments in goat. In a first experiment, a standard vitrification protocol, previously designed for sheep embryos was compared to slow freezing of goat embryos. No significant difference was observed on kidding rate (48% versus 69%, respectively), nor on embryo survival rate (35% versus 45%). Second experiment: all embryos were vitrified. After warming, embryos were either transferred directly (direct transfer), or after in vitro dilution of the cryoprotectants (conventional transfer). The kidding rate was not affected by the transfer method (38% versus 23%, respectively). However, embryo survival rate tended to be higher after direct transfer (26% versus 14%). Third experiment: OPS vitrification was compared to standard vitrification. The kidding rate was not affected (22% versus 39%, respectively), but the embryo survival rate was lower after OPS (14% versus 28%). Fourth experiment: 0.4 M sucrose was added with cryoprotectants in vitrification. The kidding rate after direct transfer was significantly enhanced after addition of sucrose (56% versus 27%, respectively), whereas embryo survival rate was not significantly affected (32% versus 18%). Fifth experiment: vitrification with sucrose supplementation was compared to slow freezing. No significant difference was observed after direct transfer on kidding rate (52% versus 31%, respectively), but embryo survival rate tended to be higher after vitrification (34% versus 2 1 %). In conclusion, our results indicate that addition of 0.4 M sucrose in association with direct transfer improves significantly the viability of goat vitrified embryos. [less ▲] Detailed reference viewed: 44 (1 ULg)The viability of in vitro produced sheep embryos is negatively affected by the presence of estradiol during in vitro maturation; ; et al in Reproduction in Domestic Animals (2005), 40(4), 92 Detailed reference viewed: 8 (2 ULg)Kidding and embryo survival rate after traditional or direct transfer of frozen/thawed goat embryons; ; et al in Proceedings of the 15th International Congress on Animal Reproduction (2004) Detailed reference viewed: 2 (0 ULg)Effet de prétraitements agoniste et antagoniste de GnRH sur la production d’embryons chez la brebis et la chèvre; ; et al in Proceedings: 11e Rencontres autour des Recherches sur les Ruminants (3R) (2004) Detailed reference viewed: 11 (2 ULg)Diagnostic et suivi de gestation chez la chèvre et la brebisMelo de Sousa, Noelita ; ; et alScientific conference (2004) Detailed reference viewed: 18 (3 ULg)Four years of induction/synchronization of estrus in dairy goats: effect on the evolution of eCG binding rate in relation with the parameters of reproductionDrion, Pierre ; ; et alin Reproduction Nutrition Development (2001), 41(5), 401-412 Ninety-eight Alpine goats of two herds were followed over 4 years in a program of annual artificial insemination after estrus induction/synchronization, including progestagen administration (vaginal ... [more ▼] Ninety-eight Alpine goats of two herds were followed over 4 years in a program of annual artificial insemination after estrus induction/synchronization, including progestagen administration (vaginal sponge) followed by prostaglandin analog and equine chorionic gonadotrophin (eCG) 48 h before sponge removal. Goats were sampled every 4 hours from the 16th to the 56th following sponge removal, for determination of LH surge and tested for estrus by the presence of a buck. Seven days after AI, endoscopic examination of the ovaries was performed to determine the number of corpus lutea. Pregnancy diagnosis was performed at day 21-22 post AI by determination of plasma progesterone and at day 40-45 by ultrasonography. Parturition, number and sex of kids were recorded. All the goats were sampled before and after each treatment, for anti-eCG antibodies screening. Statistical analysis of the results clearly established a significant effect of the treatments on anti-eCG antibodies. Time of estrus and LH surge were significantly different between herd. The antibodies significantly delayed the time of coming out of estrus as well as the time of LH surge. Two antagonistic effects were evidenced: first, the delayed of time of estrus and time of LH surge in relation with the immune reaction to eCG; secondly, the ahead of time of estrus and time of LH surge during the years of treatment, identical to both herd. The antibodies negatively influenced the percentage of ovulating females as well as kidding rate. Finally, no effect of antibodies on prolificacy was found. [less ▲] Detailed reference viewed: 58 (9 ULg)Does annual repetition of estrous induction influence the fertility of goats after A.I. at a fixed time ?Drion, Pierre ; ; et alin Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2001), 5(1), 28-29 Detailed reference viewed: 31 (9 ULg)In vitro survival of vitrified goat embryos: comparison of two vitrification methods; ; et al in Proceedings: 17e Réunion A.E.T.E. (2001) Detailed reference viewed: 2 (0 ULg)Effets de la répétition des traitements Progestagènes/PMSG chez la chèvre; ; et al (1998, April 30) Detailed reference viewed: 22 (2 ULg)Synchronization of estrus in goats: the relationship between PMSG binding in plasma, time of occurrence of estrus and fertility following artificial insemination; Remy, Benoît ; et alin Theriogenology (1996), 45(8), 1553-1559 Radioimmunoassay (RIA) was used to measure plasma eCG binding in dairy goats (n = 524) at the beginning of a progestagen/eCG treatment and 25 d after eCG administration. The eCG binding was not dependent ... [more ▼] Radioimmunoassay (RIA) was used to measure plasma eCG binding in dairy goats (n = 524) at the beginning of a progestagen/eCG treatment and 25 d after eCG administration. The eCG binding was not dependent on the age of the females but increased with the number of treatments they had previously received (3.4 % ± 4.8, N = 47 vs 9.6 % ± 13.2, N = 249; mean ± SD; P < 0.01 for goats treated 0 and 1 time vs those treated 2 to 5 times, respectively). The synchronization treatment led to an increase in the binding of eCG (7.1 % ± 10.9 before vs 28.3 ± 24.5 after treatment; P < 0.01). When eCG binding before treatment was higher than 5 % the onset of estrus was delayed: 37.9 % of goats came into estrus more than 30 h after sponge removal vs 7.4 % when eCG binding was lower than 5 % (P < 0.01). Fertility was significantly decreased when eCG binding was higher than 10 %. These results show that the repetition of treatment with eCG to induce estrus in goats increases eCG binding. This could explain the lowered efficiency of the hormonal treatment to synchronize estrus and the associated decrease in fertility when goats are inseminated at a predetermined time. [less ▲] Detailed reference viewed: 41 (0 ULg) |
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