  Indigenous Muscovy ducks in Congo-Brazzaville. 2. Preliminary observations on indigenous Muscovy ducks reared under moderate inputs in Congolese conditions; ; Leroy, Pascal  et alin Tropical Animal Health and Production (2007), 39(2), 123-129 Detailed reference viewed: 8 (0 ULg)Indigenous Muscovy ducks in Congo-Brazzaville. 1. A survey of indigenous Muscovy duck management in households in Dolisie City; ; Leroy, Pascal in Tropical Animal Health and Production (2007), 39(2), 115-122 A cross-sectional study by means of a questionnaire with open-ended questions and multiple-choice questions was used to collect data on the profile of duck keepers, husbandry practices, and performances ... [more ▼] A cross-sectional study by means of a questionnaire with open-ended questions and multiple-choice questions was used to collect data on the profile of duck keepers, husbandry practices, and performances, opportunities and constraints of Muscovy duck breeding in households (n = 88) in Dolisie city (Congo-Brazzaville). The study confirmed the common observations on traditional poultry keeping such as scavenging during the day and housing overnight. The flock size (7.7 ± 3 ducks per unit) showed no specialization of husbandry (100% of surveyed flocks were kept for simultaneous production of ducklings, meat and eggs) and a high drake-to-duck ratio (1:3). The hatchability was close to 80.5% ± 13%, whereas the average number of eggs was 13.2 ± 5 per clutch. In addition, a high mortality (80%) was observed in ducklings, which was due to poor feeding, lack of veterinary care and housing conditions. Eggs and live ducks were sold by duck farmers in response to the family needs rather than market price. The three most important findings were as follows: (1) duck keepers were mainly men (80% versus 20% of women); (2) there was no evidence of taboo; and (3) the duck as an exotic bird was not proscribed by cultural beliefs, and therefore development of the Muscovy duck in Congo Brazzaville should be unhindered. [less ▲] Detailed reference viewed: 24 (3 ULg)Correlation between a proteolytic method and a radioimmunoassay for porcine serum pepsinogen concentrations; ; et al in Research in Veterinary Science (2006), 80(3), 260-266 The measurement of serum pepsinogen concentrations by enzymatic method and immunoassay provides diagnostic values and should be helpful in the detection of gastric diseases related to a rise of blood ... [more ▼] The measurement of serum pepsinogen concentrations by enzymatic method and immunoassay provides diagnostic values and should be helpful in the detection of gastric diseases related to a rise of blood pepsinogen. In the present study, the correlation between a conventional enzymatic method and a recently developed radioimmunoassay (RIA) for serum pepsinogen A was investigated. A total of 123 sera samples of porcine foetuses (n = 28), adult healthy pigs (n = 56), pigs with parakeratosis (n = 25) and pigs with ulceration of the pars oesophagea (n = 14) were tested. Overall, there was a slight correlation between the two methods (r = 0.60). In relation to individual animal groups, the correlations (r) were 0.39 (P>0.05), 0.74 (P<0.001), 0.19 (P>0.05) and 0.34 (P>0.05) in foetuses, healthy pigs, pigs with parakeratosis and pigs with ulcers, respectively. In both methods, pepsinogen concentrations (means+/-SE) were significantly higher (P<0.05) in pigs with parakeratosis (1778 +/- 86.00 mUTyr/L; 690 +/- 53.00 ng/mL) and in pigs with ulcers (2026 +/- 153.00 mUTyr/L; 1747 +/- 94.00 ng/mL) when compared to healthy pigs (935 +/- 58.00 mUTyr/L; 275 +/- 35.00 ng/mL). The proteolytic method gave a significant increased activity (P<0.05) in foetuses (1150 +/- 82.00 mUTyr/L) vs. (935 +/- 58.00 mUTyr/L) in healthy adult pigs, indicating an additional proteolytic activity in the sera of foetuses or neonates. [less ▲] Detailed reference viewed: 11 (0 ULg)Survey of gastric lesions and blood pepsinogen levels in pigs in Burkina Faso; ; et al in Veterinary Research Communications (2003), 27(8), 595-602 The purpose of the study was to investigate the prevalence of gastric lesions and to provide diagnostic values for serum pepsinogen in non-infected pigs and in pigs with gastric disease. In an abattoir ... [more ▼] The purpose of the study was to investigate the prevalence of gastric lesions and to provide diagnostic values for serum pepsinogen in non-infected pigs and in pigs with gastric disease. In an abattoir survey, the pepsinogen concentrations were measured in the serum from 62 non-infected pigs, 33 pigs with gastric lesions and 17 pigs infected with Hyostrongylus rubidus, using a specific radioimmunoassay (RIA). The mean (+/-SE) pepsinogen concentrations in the serum of non-infected pigs, in pigs with gastric ulcers, and in pigs with a heavy H. rubidus infection were 630.8 +/- 39.2 ng/ml, 1084.5 +/- 166.2 ng/ml and 1095.2 +/- 102.3 ng/ml, respectively (p < 0.05). Because of the higher concentrations of pepsinogen in the blood of pigs with gastric ulcers or parasitic infections, it is suggested that the measurement of serum pepsinogen by RIA may be an effective biochemical approach to the diagnosis of chronic gastric disorders in pigs. [less ▲] Detailed reference viewed: 14 (0 ULg)Development and validation of a specific radioimmunoassay for equine osteocalcin; Sulon, Joseph ; et alin Domestic Animal Endocrinology (2003), 24(1), 31-41 This study describes for the first time the development and validation of a sensitive and specific radioimmunoassay (RIA) for equine osteocalcin (OC) quantification using purified equine OC as standard ... [more ▼] This study describes for the first time the development and validation of a sensitive and specific radioimmunoassay (RIA) for equine osteocalcin (OC) quantification using purified equine OC as standard, tracer, and immunogen for antibody formation in rabbits. The assay allowed to measure equine serum OC levels with a sensitivity of 0.2 ng/mL. Immunoreactive serum OC values of clinically normal, different-aged horses ranged from 3.68 to 127.31 ng/mL. Intra- and inter-assay coefficients of variation (CV) were 6.2 and 8.2%, respectively. Serial equine scrum sample dilutions were linear. The recovery of equine OC from equine serum samples ranged from 93.88 to 107.9%. There was a tight correlation between OC values measured with the equine- specific OC RIA and two commercially available bovine-specific OC RIA kits. However, highest serum OC values were obtained with the equine-specific OC RIA. In conclusion, our equine-specific OC RIA is sensitive, linear, accurate, precise, and reproducible. The assay allowed to quantify OC in equine serum samples and might, therefore, be used to monitor equine osteoblast activity associated with bone diseases, exercise, therapy forms or diet. [less ▲] Detailed reference viewed: 35 (1 ULg)Double radial immunodiffusion as a tool to identify pregnancy-associated glycoproteins in ruminant and nonruminant placentae; Melo de Sousa, Noelita ; et alin Theriogenology (2003), 59(5-6), 1291-1301 Pregnancy-associated glycoproteins (PAGs) are antigens synthesized in the superficial layers of the ruminant trophoblast. Initially, they were identified either as proteins released into the maternal ... [more ▼] Pregnancy-associated glycoproteins (PAGs) are antigens synthesized in the superficial layers of the ruminant trophoblast. Initially, they were identified either as proteins released into the maternal bloodstream (where they have applications in pregnancy diagnosis) (PAG1) or as molecules binding to the LH receptor (PAG2). In this study, double radial immunodiffusion was used to test the ability of antisera raised against different PAG molecules (bovine, ovine and caprine) to react with placental extracts from nonruminants (rabbit, cat, mouse, pig, and wild pig) and ruminants (cow, ewe, and goat). Placental extracts from all nonruminants tested except rabbit reacted with anti bovine PAG2 (anti-boPAG2). Extracts of ruminant placentas reacted with different antisera, confirming the expression of various PAG molecules. According to the time at which the placentas were collected (early or middle pregnancy), the reaction differed as regards the thickness, position, and number of precipitation lines, suggesting that PAG expression varies as pregnancy progresses. Bos indicus and Bos taurus placental extracts exhibited different reactions with anti-boPAG2: a single precipitation line in the former case and two lines in the latter. This suggests differential expression of boPAG2 related glycoproteins in these two subspecies. [less ▲] Detailed reference viewed: 23 (0 ULg) Isolation and Radioimmunoassay Formation of Equine Osteocalcin- Preliminary Results; ; et al in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2002), 6(1), 8-9 Detailed reference viewed: 2 (0 ULg)Pregnancy-associated glycoproteins isolated from the ewe placenta removed at two different stages of gestation.; ; Melo de Sousa, Noelita et alin Proceedings of the 7e Journée de Rencontre Bioforum (2002) Detailed reference viewed: 12 (2 ULg)Investigations nouvelles sur les protéines associées à la gestation chez la brebis en vue d’application clinique.; ; Melo de Sousa, Noelita et alPoster (2002) Detailed reference viewed: 6 (0 ULg)Pepsinogen and progesterone concentration during pregnancy in sows.; ; et al in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2002), 6(1), 12-13 Detailed reference viewed: 8 (1 ULg)Purification and characterization of a pregnancy-associated glycoprotein (ovPAG-6) from sheep placenta removed between 66 to 100 days of gestation.; ; Melo de Sousa, Noelita et alin Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2002), 6(1), 12-13 Detailed reference viewed: 7 (0 ULg)Pregnancy-associated glycoprotein profiles of 5 heifers measured by three radioimmunoassay systems.; ; et al in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2002), 6(1), 6-7 Detailed reference viewed: 3 (0 ULg)Aspartic proteinase members secreted by the ruminant placenta: Specificity of three radioimmunoassay systems for the measurement of pregnancy-associated glycoproteins; ; Drion, Pierre et alin Reproduction in Domestic Animals (2002), 37(6), 324-329 Pregnancy-associated glycoproteins (PAGs) isolated from the placenta of various ruminant species are enzymatically inactive members of the aspartic proteinase family. The measurement of these proteins in ... [more ▼] Pregnancy-associated glycoproteins (PAGs) isolated from the placenta of various ruminant species are enzymatically inactive members of the aspartic proteinase family. The measurement of these proteins in the maternal blood can be a good indicator of the presence of a live embryo. As certain aspartic proteinases are present in biological fluids in physiological and pathological conditions at various concentrations, it was necessary to determine the specificity of three radioimmunoassay (RIA) systems currently used for the detection of PAG molecules. Commercially available members of the aspartic proteinase family like pepsinogen, pepsin, chymosin, rennet, cathepsin D and renin were tested in a wide concentration range (10 ng/ml - 1 mg/ml). Pepsinogen cross-reacted in RIA 1, RIA 2 and RIA 3 over 1 mg/ml, 50 microg/ml and 500 microg/ml concentrations, respectively. In the presence of pepsin, cross-reaction was observed in RIA 1, RIA 2 and RIA 3 over 1 mg/ml, 500 microg/ml and 1 mg/ml concentrations, respectively. Chymosin and rennet could cross-react in RIA 2 and RIA 3, while renin and cathepsin D did not decrease the binding of the tracer to antisera more, than that of the minimal detection limit. As the plasma/serum concentrations of the examined aspartic proteinases reported in the literature were outside the concentration range where cross-reaction was observed, it can be concluded that these RIA systems were specific for the detection of PAGs in biological fluids. [less ▲] Detailed reference viewed: 19 (2 ULg)Isolation of the pregnancy-associated glycoprotein 1 (PAG-1) from zebu (Bos indicus) placenta.Melo de Sousa, Noelita ; ; et alin Proceedings of the 16th Scientific Meeting of the European Embryo Transfer Association. (2000) Detailed reference viewed: 8 (0 ULg)Pregnancy-associated glycoproteins (PAG) in Bos taurus taurus and Bos taurus indicus.; Melo de Sousa, Noelita ; et alin Theriogenology (2000), 53 Detailed reference viewed: 8 (0 ULg)Comparison of native and inherited immunoglobulins in rabbits immunized against bovine pregnancy-associated glycoprotein-1 (boPAG-1).Melo de Sousa, Noelita ; ; et alin Pflügers Archiv : European Journal of Physiology (2000), 440 Detailed reference viewed: 4 (0 ULg)Normal ongoing pregnancy in rabbit immunised against bovine pregnancy-associated glycoprotein-1 (boPAG-1).; Melo de Sousa, Noelita ; et alin Pflügers Archiv : European Journal of Physiology (2000), 440 Detailed reference viewed: 3 (0 ULg)The pregnancy-associated glycoproteins in sheep: new investigations by using the Ouchterlony technique.; ; et al in Proceedings of the 15th Scientific Meeting of the European Embryo Transfer Association (1999) Detailed reference viewed: 9 (0 ULg) |
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