References of "Bajji, Mohammed"
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See detailIdentification of Metabolic Pathways Expressed by Pichia anomala KH6 in the Presence of the Pathogen Botrytis cinerea on Apple: New Possible Targets for Biocontrol Improvement
Kwasiborski, Anthony; Bajji, Mohammed; Renaut, J. et al

in PLoS ONE (2014), 9(3),

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See detailConstruction et développement d’une filière de production d’écotypes ligneux pour l’application des techniques végétales à la renaturation des berges de cours d’eau
Druart, Philippe; Leclercq, Martine; Joussemet, Marie Anne et al

in Druart, Philippe; Husson, Claude; Paul, Roger (Eds.) Renaturation des berges de cours d'eau et phytoremédiation (2013)

Bien que les techniques végétales soient d'application de longue date pour fixer les berges de cours d'eau, elles font maintenant partie d'une réflexion plus générale avec la prise en compte des besoins ... [more ▼]

Bien que les techniques végétales soient d'application de longue date pour fixer les berges de cours d'eau, elles font maintenant partie d'une réflexion plus générale avec la prise en compte des besoins écologiques des écosystèmes de rivières et le développement de l'ingénierie végétale qui régit la mise en oeuvre des renaturations. Les travaux décrits dans cet ouvrage apportent les outils permettant la traçabilité et l'évaluation comportementale d'écotypes ligneux vis-à-vis de risques sanitaires connus (principalement la maladie de l'aulne) ou de pollutions attendues (diffusion de métaux lourds) tout en préservant leur capacité d'adaptation naturelle. Par l'établissement de collections d'aulnes, de saules ou de frênes autochtones et une multiplication végétative respectueuse de la diversité locale, les ressources génétiques de toute une région transfrontalière sont rendues disponibles pour tout programme de renaturation de berges de cours d'eau ou de zones écologiquement équivalentes à réhabiliter dans les bassins de la Meuse, du Rhin et de l'Escaut. Cet ouvrage s'adresse aux gestionnaires de cours d'eau, aux éco-conseillers du monde rural ou urbain, aux étudiants en sciences du vivant, ainsi qu'à toute personne s'intéressant à l'écosystème « rivière » et à la qualité de l'eau. Les stratégies éveloppées peuvent trouver application en d'autres lieux ou avec d'autres essences. [less ▲]

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See detailThe control of postharvest blue and green molds of citrus in relation with essential oil-wax formulations, adherence and viscosity.
Kouassi, Kouadio Hugues Sosthène ULg; Bajji, Mohammed; Jijakli, Haissam ULg

in Postharvest Biology & Technology (2012), 73

The use of wax coatings enriched with antifungals has significantly contributed to quality maintaining of harvested citrus fruit. On the other hand, interest in essential oils (EOs) as an alternative to ... [more ▼]

The use of wax coatings enriched with antifungals has significantly contributed to quality maintaining of harvested citrus fruit. On the other hand, interest in essential oils (EOs) as an alternative to synthetic fungicides has recently gained momentum. In this study, Cinnamomum zeylanicum EO was incorporated into a variety of commercial citrus waxes (shellac, carnauba, paraffin and polyethylene). The biological activity of these formulations against green and blue rots as well as their viscosity and adherence to the orange fruit surface were evaluated. Excellent disease control was achieved with C. zeylanicum EO incorporated in shellac and/or carnauba wax compared to other EO–wax formulations. Disease control by EO–waxes seems to depend not only on the volume that remains on the fruit skin, but also, probably on the retention of EO components on the fruit. Other factors such as formulation solubility, permeability to gases, and compatibility between EO compounds and those of waxes may also be involved in the improvement of EO efficacy. The present study may therefore allow a careful selection of ppropriate waxes for the elaboration of effective EO–wax formulations. [less ▲]

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See detailCharacterization of a novel chitinase from a moderately halophilic bacterium, Virgibacillus marismortui strain M3-23
Essghaier, Badiaa; Hedi, Abdeljabbar; Bajji, Mohammed et al

in Annals of Microbiology (2012), 62(2), 835-841

A new chitinase produced by the moderately halophilic bacterium Virgibacillus marismortui strain M3- 23 was identified and characterized. Distinguishable characteristics of high activity and stability at ... [more ▼]

A new chitinase produced by the moderately halophilic bacterium Virgibacillus marismortui strain M3- 23 was identified and characterized. Distinguishable characteristics of high activity and stability at different pH, temperatures and salinity of M3-23 chitinase are reported. Analysis of the catalytic domain sequence from the enzyme highlighted its relationship to glycosyl hydrolase family 18. Comparison of the deduced chitinase sequence from strain M3-23 to known chitinases from Bacillus species showed low similarity (82%), suggested its novelty. This is the first report of the characterization of chitinase from the species V. marismortui. The halo- and thermo-tolerant nature of the chitinolytic enzyme allows its potential use in agricultural and industrial applications. [less ▲]

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See detailRôle des acides aminés dans l'activité antagoniste d'Aureobasidium Pullulans vis-à-vis de la pourriture bleue des pommes en post-récolte
Krimi Bencheqroun, Sanae; Bajji, Mohammed; Laghilili, Mustapha et al

Conference (2011, March 23)

Aureobasidium pullulans (souche Ach1-1) a montré une grande efficacité vis-à-vis de Penicillium expansum agent de la pourriture bleue des pommes en conservation. Le présent travail a été réalisé dans le ... [more ▼]

Aureobasidium pullulans (souche Ach1-1) a montré une grande efficacité vis-à-vis de Penicillium expansum agent de la pourriture bleue des pommes en conservation. Le présent travail a été réalisé dans le cadre de l’analyse des mécanismes d’action qui sont les plus impliqués dans son activité antagoniste. Dans des essais in vivo sur pommes blessées, l’effet protecteur de la souche Ach1-1 vis-à-vis de P. expansum a été significativement affaibli par l’ajout dans les blessures de concentrations élevées en acides aminés. L’importance de la réduction étant dépendante de la concentration appliquée, a montré que la compétition pour les acides aminés joue un rôle important dans la suppression de P. expansum. Ce résultat a été appuyé par l’analyse biochimique de la cinétique de l’épuisement des acides aminés dans les blessures des pommes qui a dévoilé que ces composés et particulièrement la sérine, la glycine et l’acide glutamique sont mieux métabolisés par la souche antagoniste que par le pathogène. L’ajout en excès de ces trois acides aminés individuellement dans les blessures des pommes a fortement réduit l’efficacité de la souche Ach1-1 vis-à-vis de P. expansum. Ainsi, ces acides aminés semble être parmi les éléments les plus limitants dans le mécanisme de la compétition. Ces résultats indiquent que l’antagoniste A. pullulans souche Ach 1-1 exerce une activité fongistatique plus que fongicide vis-à-vis de P. expansum et agit par une compétition efficace pour les éléments nutritifs limitants des blessures des pommes et particulièrement les acides aminés sans affecter la viabilité du pathogène. [less ▲]

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See detailEvaluation of two protein extraction protocols for Pichia anomala proteome analysis.
Bajji, Mohammed; Delaplace, Pierre ULg; Mauro, Sergio et al

Poster (2009, May 19)

Pichia anomala (strain Kh6) was isolated from the surface of apple fruits and selected for its high and reliable biocontrol activity against Botrytis cinerea and Penicillium expansum. Its main modes of ... [more ▼]

Pichia anomala (strain Kh6) was isolated from the surface of apple fruits and selected for its high and reliable biocontrol activity against Botrytis cinerea and Penicillium expansum. Its main modes of action have until now been studied using essentially microbiological and molecular approaches. The study continues now using the proteomic approach and considering the in situ P. anomala/B. cinerea/apple interaction. Two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) is one of the most powerful tools used for proteomic analysis. It combines two sequential separation steps, the first dimension via isoelectric focusing (IEF) and the second one by SDS PAGE. Although recent advances in 2-D PAGE, the extraction of the whole proteome and the removal of interfering contaminants still limit its application. Sample preparation constitutes indeed a critical influential step for IEF which in turn affects 2-D gel quality. The objective of the present work was thus to develop an effective protein extraction protocol designed for 2-D PAGE analysis of the proteome of P. anomala strain Kh6. As a starting point, two contrasting protein extraction protocols were chosen to be evaluated in terms of protein yield and one-dimensional (1-D) SDS PAGE and 2-D PAGE gel patterns. The first protocol uses a urea/thiourea-based lysis buffer whereas the second protocol utilizes a hot SDS-based lysis buffer with an additional precipitation step. The comparison model used consisted of apples treated with strain Kh6 alone (K) and apples first treated with Kh6 and then inoculated with B. cinerea conidia (KB). Growth kinetics of strain Kh6 on wounded apples was determined and found to be not affected by the presence of B. cinerea conidia. Proteins were extracted from yeast pellets collected at both the exponential and stationary phases of strain Kh6 growth on apples. The evaluation of both extraction protocols indicates that more proteins were extracted with the SDS protocol and, according to 1-D assays, higher molecular weight proteins were obtained with the ‘urea/thiourea’ protocol and, regardless of the protocol used, more bands were obtained during the exponential phase. 2-D assays are currently underway and the corresponding results will be presented. [less ▲]

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See detailHigh salt-tolerant protease from a potential biocontrol agent bacillus pumilus M3-16
Essghaier, Badiaa; Bajji, Mohammed; Jijakli, Haissam ULg et al

in Annals of Microbiology (2009), 59(3), 553-558

In this paper, we investigate the characterization and evaluation of the antifungal protease activity from a halotolerant strain M3-16 of Bacillus pumilus, earlier isolated from a shallow salt lake in ... [more ▼]

In this paper, we investigate the characterization and evaluation of the antifungal protease activity from a halotolerant strain M3-16 of Bacillus pumilus, earlier isolated from a shallow salt lake in Tunisia. Protease enzyme was highly induced by the pathogen tested in vitro (27.4 U/ml). This is the first report on high salt-tolerant protease from B. pumilus, since it was active at high salinity (from 5 to 30% NaCl, w/v) as well as in the absence of salinity. This enzyme showed optimal activity at 60 °C and pH 8. At 80 °C and 30 min, the enzyme retained up to 91% and it showed stability over a wide pH range (from pH 5 to 11). The enzyme was found to be monomer with an estimated molecular mass of 31 kDa. The amino acid sequence showed high similarity (94%) to ATP-dependent protease from B. pumilus strain ATCC 7061. Thus, our alkaline thermostable and high salt-tolerant protease induced by a phytopathogenic fungus, could be useful for application in diverse areas such as biotechnology alimentary and agronomy industries. [less ▲]

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See detailcDNA-AFLP analysis of Candida oleophila (strain O) genes differentially expressed during the biocontrol of Botrytis cinerea on harvested apples
Bajji, Mohammed; Jijakli, Haissam ULg

in Bulletin OILB/SROP = IOBC/WPRS Bulletin (2009), 43

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See detailDevelopment of SCAR markers and a semi-selective medium for the quantification of strains Ach 1-1 and 1113-5, two Aureobasidium pullulans potential biocontrol agents.
El-Hamouchi, Adil; Bajji, Mohammed; Friel, Damien et al

in Postharvest Biology & Technology (2008), 50(2-3), 216-223

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