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See detailApplication of NIR hyperspectral imaging combined to chemometrics to assess the impact of tillage on the root system development of a winter wheat crop
Fraipont, Guillaume ULg; Eylenbosch, Damien ULg; Baeten, Vincent et al

Poster (2016, July)

This poster presents de results of a study of the influence of tillage on the root development of a winter wheat crop. The originality of this research lies in the application of an innovative root ... [more ▼]

This poster presents de results of a study of the influence of tillage on the root development of a winter wheat crop. The originality of this research lies in the application of an innovative root quantification method based on the near infrared hyperspectral imaging. [less ▲]

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See detailLifetime stability study of a NIR hyperspectral imaging system
Eylenbosch, Damien ULg; Bodson, Bernard ULg; Baeten, Vincent et al

Conference (2016, July)

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See detailCharacterization of the impact of tillage on the root development and distribution of crop residues of a winter wheat crop by the use of NIR hyperspectral imaging
Fraipont, Guillaume ULg; Eylenbosch, Damien ULg; Fernández Pierna, Juan Antonio et al

Poster (2016, May 20)

The objectives of the study were to show the impact of tillage and crop residues management on the distribution of crop residues and root system of a winter wheat crop in the 0-30 cm soil profile.This ... [more ▼]

The objectives of the study were to show the impact of tillage and crop residues management on the distribution of crop residues and root system of a winter wheat crop in the 0-30 cm soil profile.This poster shows the results obtained during one crop year (2011-2012). This study is allowed by a rapid and innovative method of root system quantification based on the near infrared hyperspectral Imaging. [less ▲]

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Peer Reviewed
See detailIn situ analysis of lipid oxidation in oilseedbased food products using near-infrared spectroscopy and chemometrics: The sunflower kernel paste (tahini) example
Mureșan; Danthine, Sabine ULg; Mureșan, Andruța Elena et al

in Talanta (2016), 155

A new near-infrared (NIR) spectroscopic method was developed for the analytical measurement of lipid oxidation in sunflower kernel paste (tahini), which was chosen as an example of a complex oilseed-based ... [more ▼]

A new near-infrared (NIR) spectroscopic method was developed for the analytical measurement of lipid oxidation in sunflower kernel paste (tahini), which was chosen as an example of a complex oilseed-based food product. The NIR spectra of sunflower tahini were acquired for the extracted fat phase (EFP) and for the intact sunflower tahini (IST) samples during controlled storage. The best peroxide value (PV) calibration models were considered suitable for quality control (ratio of performance of deviation [RPD] >5). The best PV partial least squares (PLS) model result for EFP (RPD 6.36) was obtained when using standard normal variate (SNV) and the Savitzky-Golay first derivative in the 1140-1184 nm, 1388-1440 nm and 2026-2194 nm regions. In the case of IST spectra, the best PV models (RPD 5.23) were obtained when either multiple scattering correction (MSC) or SNV were followed by the Savitzky-Golay second derivative for the 1148-1180 nm and 2064-2132 nm regions. There were poor correlations between the NIR-predicted values and the reference data of the panisidine value (pAV) for both EFP and IST. Overall, the results obtained showed that NIR spectroscopy is an appropriate analytical tool for monitoring sunflower paste PV in situ. Due to the nonexistence of the extraction step, it demonstrates a unique and substantial advantage over presently known methods. Based on these results it is strongly recommended that, when using NIR PLS models to assess lipid oxidation in situ in similar oilseedbased food products (e.g., sesame tahini, hazelnut and cocoa liquor used for chocolate production, peanut butter, hazelnut, almond, pistachio spreads), suitable calibration sets containing samples of different particle sizes and stored at different temperatures be selected. [less ▲]

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See detailCharacterization of the impact of tillage and nitrogen fertilization on the root development of a winter wheat crop by use of NIR hyperspectral imaging combined to chemometrics
Fraipont, Guillaume ULg; Eylenbosch, Damien ULg; Fernández Pierna, Juan Antonio et al

Poster (2016, March 16)

The objective of this study is to characterize the impact of tillage management and nitrogen fertilization on the root system development of a winter wheat crop. To reach this objective, an innovative ... [more ▼]

The objective of this study is to characterize the impact of tillage management and nitrogen fertilization on the root system development of a winter wheat crop. To reach this objective, an innovative root quantification method that combines NIR hyperspectral imaging (NIR-HSI) and chemometric tools will be applied. [less ▲]

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See detailComparaison des performances des modèles d'analyse discriminante PLS et SVM
Eylenbosch, Damien ULg; Fernández Pierna, Juan Antonio; Baeten, Vincent et al

Poster (2016, January 19)

Les performances des modèles d’analyses discriminantes PLS (Partial Least Squares) et SVM (Support Vector Machines) ont été comparées dans une étude qui avait pour objectif de discriminer des spectres ... [more ▼]

Les performances des modèles d’analyses discriminantes PLS (Partial Least Squares) et SVM (Support Vector Machines) ont été comparées dans une étude qui avait pour objectif de discriminer des spectres proches infrarouges sur base d'images hyperspectrales de racines et de pailles de froment en vue de les quantifier. [less ▲]

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Peer Reviewed
See detailChemometrics and vibrational spectroscopy for the detection of melamine levels in milk
Fernandez Pierna, Juan; Vincke, Damien; Baeten, Vincent et al

Poster (2016, January 19)

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See detailApplication of a standardisation procedure on an international network of MIR instruments for milk analysis
Grelet, Clément ULg; Fernandez Pierna, Juan A.; Dardenne, Pierre et al

Poster (2016, January)

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See detailIdentification of specific bovine blood biomarkers with a non-targeted approach using HPLC ESI tandem mass spectrometry
Lecrenier, Marie-Caroline ULg; Marbaix, Hélène; Dieu, Marc et al

in Food Chemistry (2016), 213

Animal by-products are valuable protein sources in animal nutrition. Among them are blood products and blood meal, which are used as high-quality material for their beneficial effects on growth and health ... [more ▼]

Animal by-products are valuable protein sources in animal nutrition. Among them are blood products and blood meal, which are used as high-quality material for their beneficial effects on growth and health. Within the framework of the feed ban relaxation, the development of complementary methods in order to refine the identification of processed animal proteins remains challenging. The aim of this study was to identify specific biomarkers that would allow the detection of bovine blood products and processed animal proteins using tandem mass spectrometry. Seventeen biomarkers were identified: nine peptides for bovine plasma powder; seven peptides for bovine haemoglobin powder, including six peptides for bovine blood meal; and one peptide for porcine blood. They were not detected in several commercial compound feed or feed materials, such as blood by-products of other animal origins, milk-derived products and fish meal. These biomarkers could be used for developing a species-specific and blood-specific detection method. [less ▲]

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See detailLinseed oil presents different patterns of oxidation in real-time and accelerated aging assays
Douny, Caroline ULg; Razanakolona, Rina; Ribonnet, Laurence et al

in Food Chemistry (2016), 208

This study aimed at verifying if the hypothesis that one day at 60 °C is equivalent to one month at 20 °C could be confirmed during linseed oil aging for 6 months at 20 °C and 6 days at 60 °C using the ... [more ▼]

This study aimed at verifying if the hypothesis that one day at 60 °C is equivalent to one month at 20 °C could be confirmed during linseed oil aging for 6 months at 20 °C and 6 days at 60 °C using the “Schaal oven stability test”. Tests were conducted with linseed oil supplemented or not with myricetin or butyl-hydroxytoluene as antioxidants. Oxidation was evaluated with the peroxide and p-anisidine values, as well as the content in conjugated dienes and aldehydes. All four indicators of oxidation showed very different kinetic behaviors at 20 and 60 °C. The hypothesis is thus not verified for linseed oil, supplemented or not with antioxidant. In the control oil, the conjugated dienes and the peroxide value observed were respectively of 41.8 ± 0.8 Absorbance Unit (AU)/g oil and 254.3 ± 5.8 meq. O2/kg oil after 6 months at 20 °C. These values were of 18.2 ± 1.3 AU/g oil and 65.2 ± 20.3 meq. O2/kg after 6 days at 60 °C. [less ▲]

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See detailUse of a multivariate moving window PCA for the untargeted detection of contaminants in agro-food products, as exemplified by the detection of melamine levels in milk using vibrational spectroscopy.
Fernandez Pierna, Juan A; Vincke, Damien; Baeten, Vincent et al

in Chemometrics and Intelligent Laboratory Systems (2016)

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See detailInnovative methods for the determination of the taxonomic origin of processed animal proteins in feed.
Veys, Pascal; Ngo Njembe, M.; Lecrenier, Marie-Caroline ULg et al

Poster (2015, November)

The use of animal by-products in feed depends on their nature defined by the type of tissue or body parts and the species of origin. Currently, the detection of unauthorised processed animal proteins ... [more ▼]

The use of animal by-products in feed depends on their nature defined by the type of tissue or body parts and the species of origin. Currently, the detection of unauthorised processed animal proteins (PAPs) is based on light microscopy and PCR methods. Light microscopy identifies structures on the basis of their morphology and enables identification of particles (such as bones, cartilages, muscle fibres,…) while PCR is able to detect and identify the presence of specific animal DNA in feed. Nevertheless, for some scenarios, even combined, these methods do not succeed in determining the taxonomic origin of the PAPs. A typical example is that of an aquafeed containing authorised porcine PAP together with dairy products: the analysis will conclude of the potential presence of ruminant PAP. Therefore, there is a need for developing methods allowing a taxonomic characterisation of visual structures such as bones fragments and muscle fibres. For the characterisation of bones, NIRM has yet demonstrated its potential. However the limitation of NIRM is when the presence of bones is reduced or absent. This study investigated the potential of NIRM for the determination of the taxonomic origin of muscle fibres. The NIRM was experimented on 2 porcine PAPs vs. 6 ruminant PAPs and 7 fishmeals all of industrial origin. Results showed that NIRM allows differentiating muscle fibres from different taxonomic origins: fish, ruminant and pig. In addition to this taxonomic classification, results also reveal differences inside taxonomic clusters of PAPs (e.g among different ruminant PAPs and porcine PAPs). The results obtained on this type of meals are promising and offer new perspectives. Tests on adulterated feeds need to be performed by NIRM prior to validation. [less ▲]

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See detailBovine blood biomarkers as a way of processed animal proteins detection in feedingstuffs
Lecrenier, Marie-Caroline ULg; Marbaix, Hélène; Veys, Pascal et al

Poster (2015, November)

The prohibition of using animal by-products in feedingstuffs depends on two factors: their nature defined by the tissue/cell type and the species of origin, and on their destination (pets, fur animals or ... [more ▼]

The prohibition of using animal by-products in feedingstuffs depends on two factors: their nature defined by the tissue/cell type and the species of origin, and on their destination (pets, fur animals or other farmed animals). Proteomics is particularly well-suited to the purpose of PAPs detection as it is a tissue and species-specific method. The aim of this study was the identification and the selection of specific peptide biomarkers using tandem mass spectrometry for the detection of bovine blood products and blood meals in animal feed. Twenty-nine samples of blood meals and blood products (plasma or haemoglobin powder) of porcine, poultry and bovine origin as well as three milk products and two fish meals were analysed using a Q TOF mass spectrometer. Vegetal feed samples adulterated with 1% or 10% of bovine plasma powder, haemoglobin powder or blood meal were also analysed to evaluate the applicability of the method. Four proteins of interest were highlighted: Alpha-2-macroglobulin, apolipoprotein A-1, serotransferrin and haemoglobin (α and β chains). From these proteins, sixteen peptides were identified as potential bovine blood biomarkers in feedingstuffs. Nine of them could be used for the detection of plasma powder and seven of them for haemoglobin powder or blood meal. The evaluation of these peptides by a search against NCBInr database revealed that some of them could also be used to detect other ruminant bloods such as ovine or caprine ones. These preliminary results are promising. Efforts are now focused to improve the protocol in order to increase the sensitivity of the method as regards the selected proteins. [less ▲]

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See detailUse of NIR hyperspectral imaging and chemometrics to quantify roots and crop residues in soil
Eylenbosch, Damien ULg; Fernández Pierna, Juan Antonio; Baeten, Vincent et al

Poster (2015, October)

Discrimination of roots and crop residues, in order to quantify them, based on their Near Infrared spectral signature and chemometrics was tested as a new rapid and reliable method.

Detailed reference viewed: 35 (1 ULg)
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See detailUse of Near Infrared Hyperspectral Imaging in studies on root systems
Eylenbosch, Damien ULg; Fernández Pierna, Juan Antonio; Fraipont, Guillaume ULg et al

Conference (2015, September 21)

Detailed reference viewed: 18 (9 ULg)
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See detailQuantification of roots and crop residues by the use of near infrared hyperspectral imaging and chemometric tools
Eylenbosch, Damien ULg; Fernández Pierna, Juan Antonio; Baeten, Vincent et al

Poster (2015, September)

In studies on root system development in fields, manual sorting of roots and crop residues extracted from soil samples before quantification is a tedious and time consuming step. Discrimination and ... [more ▼]

In studies on root system development in fields, manual sorting of roots and crop residues extracted from soil samples before quantification is a tedious and time consuming step. Discrimination and quantification of roots and crop residues based on their Near Infrared spectral signature was tested as a new rapid and reliable method. [less ▲]

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See detailGenetic variability of MIR predicted milk technological properties in Walloon dairy cattle
Colinet, Frédéric ULg; Troch, Thibault ULg; Baeten, Vincent et al

in Book of Abstracts of the 66th Annual Meeting of the European Federation of Animal Science (2015, August)

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See detailPotential of visible-near infrared spectroscopy for the characterization of butter properties
Troch, Thibault ULg; Baeten, Vincent; Dehareng, Frédéric et al

in Book of Abstracts of the 66th Annual Meeting of the European Federation of Animal Science (2015, August)

Detailed reference viewed: 20 (2 ULg)
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See detailQuality Assurance for new analytical parameters, Optimir standardisation of MIR instruments
Grelet, Clément ULg; Fernandez Pierna, Juan; Dardenne, Pierre et al

Conference (2015, June 10)

Detailed reference viewed: 10 (2 ULg)