References of "Audigié, Fabrice"
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See detailExperimental model of equine alveolar macrophage stimulation with TLR ligands.
Waldschmidt, Ingrid; Pirottin, Dimitri ULg; Art, Tatiana ULg et al

in Veterinary Immunology and Immunopathology (2013), 155(1-2), 30-37

Pulmonary diseases are common in horses and have a major economic impact on the equine industry. Some of them could be associated with an inadequate immune response in the lung, but methods to evaluate ... [more ▼]

Pulmonary diseases are common in horses and have a major economic impact on the equine industry. Some of them could be associated with an inadequate immune response in the lung, but methods to evaluate this response in horses are lacking. The aim of this study was to develop and validate an experimental model that could be applied in several physiological and pathological conditions to assess the innate immune response of equine pulmonary cells. Equine alveolar macrophages (AMs) obtained from bronchoalveolar lavages were isolated from other cells by adhesion. TLR2, 3, and 4 expression in AMs was studied and their responses to commercial ligands (respectively FSL-1, Poly(I:C), and LPS) were evaluated after determination of the appropriate dose and time of incubation. TLR responses were assessed by measuring cytokine production using (1) gene expression of TNFalpha, IFNbeta, Il-1beta, and IFNalpha by qPCR (indirect method); and (2) cytokine production for TNFalpha and IFNbeta by ELISA (direct method). TLR 2, 3, and 4 were expressed by AMs. TLR 2 stimulation with 10ng/mL of FSL-1 during 3h significantly increased IL-1beta and TNFalpha gene expression. TLR 3 stimulation with 1000ng/mL of Poly(I:C) during 1h increased IFNbeta, IFNalpha, Il-1beta and TNFalpha expression. TLR 4 stimulation with 100ng/mL of LPS during 3h increased TNFalpha, IFNbeta, and Il-1beta expression. Results obtained by ELISA quantification of TNFalpha and IFNbeta produced by AMs following stimulation during 6h were similar: FSL-1 increased TNFalpha production but not IFNbeta, Poly(I:C) and LPS increased production of IFNbeta and TNFalpha. In conclusion, pulmonary innate immunity of horses can be assessed ex vivo by measuring cytokine production following stimulation of AMs with TLR agonists. This experimental model could be applied under several conditions especially to improve the understanding of equine respiratory disease pathogenesis, and to suggest novel therapeutic opportunities. [less ▲]

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See detailTraining-induced decrease of cytokines production by equine alveolar macrophages
Waldschmidt, Ingrid ULg; Frellstedt, Linda ULg; Art, Tatiana ULg et al

in Proceedings of the 6th Congress of the European College of Equine Internal Medicine (ECEIM) (2013)

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See detailQualitative comparison of 0.27T, 1.5T and 3T Magnetic Resonance Images of the normal equine foot.
Bolen, Géraldine ULg; Audigié, Fabrice; Spriet, Mathieu et al

in Journal of Equine Veterinary Science (2010), 30(1), 9-20

Magnetic resonance (MR) imaging has become an important diagnostic tool in the investigation of foot pain in horses. The aim of this study was to qualitatively compare ex-vivo MR images of the same equine ... [more ▼]

Magnetic resonance (MR) imaging has become an important diagnostic tool in the investigation of foot pain in horses. The aim of this study was to qualitatively compare ex-vivo MR images of the same equine feet obtained at three magnetic field strengths: 0.27, 1.5, and 3 tesla (T). Ten cadaver feet were used. All feet were imaged with two high-field (HF) systems (3T, 1.5T) and with a low-field (LF) system at 0.27T designed for standing horses. Images were acquired using similar pulse sequences in all 3 MR units. MR images were subjectively evaluated by three independent experienced image analysts for image quality and clarity of visualization of individual anatomical structures using a four-point grading scale. The images from all of the examinations were considered to be of diagnostic value except for the hoof capsule where substantial artifacts were present in LF images with distortion and loss of signal at the dorsal/distal aspect of the hoof capsule in LF images. Anatomical structure scoring values of images obtained at 3T and 1.5T were significantly greater than scores of images obtained at 0.27T. Scores for images obtained at 3T were significantly higher than those for images obtained at 1.5T. Mean score differences between 1.5T and 3T were higher for cartilage of the distal interphalangeal joint and for the ungular cartilages. [less ▲]

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See detailAnatomic variations and degenerative changes in the collateral cartilages and middle and distal phalanges of the forelimb in Ardenner colts
Lejeune, Jean-Philippe ULg; Audigie, Fabrice; Schneider, Nicole ULg et al

in Journal of Equine Veterinary Science (2006), 26(3), 102-112

The anatomic variations and the degenerative changes in the collateral cartilages and middle and distal phalanges in 6 Ardenner colts were characterized by radiography, scintigraphy, and magnetic ... [more ▼]

The anatomic variations and the degenerative changes in the collateral cartilages and middle and distal phalanges in 6 Ardenner colts were characterized by radiography, scintigraphy, and magnetic resonance imaging (MRI). The radiographic changes were assessed between the ages of 16.5 and 25 months. An anatomic variation of the middle and distal phalanges was demonstrated in some of the colts. MRI examination of the 6 colts revealed an association between the deep digital flexor tendon cross-sectional area and body weight as well as foot circumference. Also, a thin collateral sesamoidean (suspensory navicular) ligament was observed subjectively. The presence of an extensive ossification of the collateral cartilages of the foot was found in these young horses. The ossification was characterized by the existence of 2 separate ossification centers, which had a tendency to unite. The radiographic interphalangeal degenerative lesions seen appear as bone remodeling of the dorsal edges of the middle and distal phalanx, on the insertion sites of collateral ligaments of the distal interphalangeal joint, the digital extensor tendon, and the distal interphalangeal joint capsule. [less ▲]

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