References of "Archambeau, Catherine"
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See detailClay and DOPA containing polyelectrolyte multilayer film for imparting anticorrosion properties to galvanized steel
Faure, Emilie ULg; Halusiak, Emilie; Farina, Fabrice et al

in Langmuir (2012), 28(5), 2971-2978

A facile and green approach is developed to impart remarkable protection against corrosion to galvanized steel. A protecting multilayer film is formed by alternating the deposition of a polycation bearing ... [more ▼]

A facile and green approach is developed to impart remarkable protection against corrosion to galvanized steel. A protecting multilayer film is formed by alternating the deposition of a polycation bearing catechol groups, used as corrosion inhibitors, with clay that induces barrier properties. This coating does not affect the esthetical aspect of the surface and does not release any toxic molecules in the environment. [less ▲]

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See detailStainless steel with robust anibacterial activiy based on a versatile and bio-inspired strategy
Faure, Emilie ULg; Lecomte, Philippe ULg; Vreuls, Christelle ULg et al

Conference (2011, September 01)

The synthesis of a poly(methacrylamide) bearing 3,4-dihydroxyphenylalanine inspired from the chemical composition of mussel adhesives will be presented. This homopolymer is designed to insure a multilayer ... [more ▼]

The synthesis of a poly(methacrylamide) bearing 3,4-dihydroxyphenylalanine inspired from the chemical composition of mussel adhesives will be presented. This homopolymer is designed to insure a multilayer film growth by covalent coupling during the layer-by-layer building with a homopolymer containing amino groups. The film cross-linking is initiated by adequately controlling both the redox state of the polymer and the pH of the solutions. This cross-linking is evidenced by solid-state 13C NMR with the occurrence of a typical signal of imine, traducing the Schiff base formation during film growth. The same coupling reaction is implemented for grafting an antibacterial peptide, Nisin, on a stainless steel substrate. Antibacterial activity against Bacillus subtilis is preserved even after long immersion time, as the result of the peptide covalent anchoring on the surface. All the processing steps, including the synthesis of the polymers and the peptide grafting, are performed in aqueous solutions under mild conditions. [less ▲]

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See detailSustainable and bio-inspired chemistry for robust antibacterial activity of stainless steel
Faure, Emilie ULg; Lecomte, Philippe ULg; Lenoir, Sandrine et al

in Journal of Materials Chemistry (2011), 21(22), 7901-7904

We report on the original synthesis of a poly(methacrylamide) bearing (oxidized) 3,4-dihydroxyphenylalanine specially designed to (i) insure film growth by covalent coupling, (ii) covalently bind an ... [more ▼]

We report on the original synthesis of a poly(methacrylamide) bearing (oxidized) 3,4-dihydroxyphenylalanine specially designed to (i) insure film growth by covalent coupling, (ii) covalently bind an antibacterial peptide and (iii) contribute to the film cross-linking that is essential for the durability of the properties. [less ▲]

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See detailBiomimetic coatings with robust antibacterial properties
Jérôme, Christine ULg; Cécius, Michaël; Faure, Emilie ULg et al

Conference (2010, July 01)

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See detailSingle molecule interactions of bio-adhesive-inspired polymers with inorganic and organic surfaces
Willet, Nicolas ULg; Giamblanco, Nicoletta ULg; Faure, Emilie ULg et al

Conference (2010, July)

Numerous living creatures have developed adhesion strategies to stick to inorganic or organic surfaces in wet environments. A classic example of permanent bioadhesion is exemplified by mussels, which ... [more ▼]

Numerous living creatures have developed adhesion strategies to stick to inorganic or organic surfaces in wet environments. A classic example of permanent bioadhesion is exemplified by mussels, which secrete adhesive proteins containing a high concentration of 3,4-dihydroxyphenylalanine (DOPA) 1, 2. The catechol species of DOPA are thought to be responsible for the strong adhesion to inorganic surfaces, whereas the oxidized o-quinone species trigger the cross-linking of the glue, ensuring cohesion. The unoxidized form of DOPA is known to adhere to a large variety of inorganic surfaces, although the adhesion mechanism is not yet fully understood.3, 4 A clear understanding is however essential for the design of synthetic adhesive polymers required in many surface science applications. Here we investigate at the single-molecule level the interaction forces between AFM tips coated with bio-inspired polymers and a variety of inorganic and organic surfaces. We prepared polymers bearing several amounts of DOPA units and covalently attached them to AFM tips following our previously published strategy.5, 6 They were homo-or co-polymers and were cross-linked or not. These original bio-inspired tips were used to perform single-molecule force spectroscopy on a range of model, as well as industrial such as stainless and galvanized steel, substrates. The specific interaction forces measured in water were compared with the ones exerted by the same polymers without DOPA. It was found that, depending on the nature of the substrate, the presence of DOPA strongly, or only slightly, increases the interaction forces with the surface. We also investigated the influence of the oxidation state of the catechol species on the intensity of the interaction forces. Again, this influence is strongly related to the nature of the substrate. Finally, we studied the effect of polymer cross-linking on the adhesive interactions. 1. Waite, J. H.; Tanzer, M. L. Science 1981, 212, 1038. 2. Waite, J. H.; Housley, T. J.; Tanzer, M. L. Biochemistry 1985, 24, 5010. 3. Lee, H.; Scherer, N. F.; Messersmith, P. B. Proc. Natl. Acad. Sci. U.S.A. 2006, 103, 12999. 4. Wang, J.; Nawaz Tahir, M.; Kappl, M.; Tremel, W.; Metz, N.; Barz, M.; Theato, P.; Butt, H.-J. Adv. Mater. 2008, 20, 3872. 5. Gabriel, S.; Jérôme, C; Jérôme, R.; Fustin, C.-A.; Pallandre, A.; Plain, J.; Jonas, A. M.; Duwez, A.-S. J. Am. Chem. Soc. 2007, 129, 8410. 6. Cecchet, F.; Lussis, P.; Jérôme, C.; Gabriel, S.; Silva-Goncalves, E.; Jérôme, R.; Duwez, A.-S. Small 2008, 4, 1101. [less ▲]

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See detailNanocoatings of inorganic surfaces by molecular biomimetic
Vreuls, Christelle ULg; Genin, Alexis ULg; Zocchi, Germaine ULg et al

Poster (2010, June 30)

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See detailA green and refillable antibacterial coating for stainless steel
Faure, Emilie ULg; Charlot, Aurélia; Sciannaméa, Valérie et al

Poster (2010, June 29)

Because of its resistance to corrosion and chemicals, relevant mechanical and esthetical properties, stainless steel (SS) is widely used in the daily life (food industry, household appliances, surgery … ... [more ▼]

Because of its resistance to corrosion and chemicals, relevant mechanical and esthetical properties, stainless steel (SS) is widely used in the daily life (food industry, household appliances, surgery …). However, SS is unable to prevent bacteria from adhering, proliferating and forming a resistant biofilm when ageing. Therefore, surface modification is needed for providing durable antibacterial properties. We report here on an all-in-one approach to prepare refillable antimicrobial films using the layer-by-layer deposition of polyelectrolytes. Specifically designed biocidal multilayered polyelectrolyte films that bear 3,4-dihydroxyphenylalanine (DOPA), known as a promoter of adhesion to inorganic surfaces, were deposited onto SS. DOPA was incorporated in the polycationic chains by radical copolymerisation of N-methacrylated DOPA with the quaternary ammonium salt of 2-(dimethylamino)ethyl methacrylate (DMAEMA+). In order to boost the antibacterial activity of the polycationic layer, AgNO3 was added to the aqueous solution of P(DOPA)-co-P(DMAEMA+), which resulted in the in-situ formation of silver based nanoparticles, sources of biocial Ag+. The layer-by-layer deposition of aqueous P(DOPA)-co-P(DMAEMA+)/AgCl/Ag0 suspension and aqueous solution of poly(styrene sulfonate) provides high antibacterial activity against Gram-negative E. Coli bacteria. Moreover, after silver depletion, films retain some antimicrobial activity, thanks to ammonium groups of the copolymer. We also show that the antibacterial activity of the films can then be easily re-boosted. [less ▲]

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See detailA robust antibacterial coating for stainless steel
Faure, Emilie ULg; Charlot, Aurélia; Sciannaméa, Valérie et al

Conference (2010, March 23)

Because of corrosion, chemical resistance, relevant mechanical and esthetical properties, stainless steel is widely used in the daily life, not only in the building industry but also in the food industry ... [more ▼]

Because of corrosion, chemical resistance, relevant mechanical and esthetical properties, stainless steel is widely used in the daily life, not only in the building industry but also in the food industry, the appliances or in the medical field, including implants in orthopedic surgery. However, stainless steel is unable to prevent bacteria from adhering, proliferating and forming a resistant biofilm. Therefore, surface modification is needed for providing the metal surface with antibacterial properties. The scientific literature is very rich in describing various methods for imparting antibacterial properties to different inorganic supports. However, the activity of the coating is generally time limited by the diffusion of the biocide in the environment. Novel robust and stable antibacterial coatings on stainless steel are thus highly desirable for the durability of the functionality. In this communication, we report on an all-in-one approach to prepare robust antimicrobial films on stainless steel using the layer-by-layer deposition of polyelectrolytes. Novel biocidal multilayered polyelectrolyte films in which the polycationic layer is silver loaded and bears 3,4-dihydroxyphenylalanine (DOPA), known as a promoter of adhesion to inorganic surfaces, were deposited onto stainless steel. DOPA was incorporated in the polycationic chains by radical copolymerisation of N-methacrylated DOPA with the commercially available quaternary ammonium salt of 2-(dimethylamino)ethyl methacrylate (DMAEMA+). Polystyrene sulfonate (PSS) was the polyanionic constituent of the films. In order to boost the antibacterial activity of the polycationic layer, AgNO3 was added to the aqueous solution of P(DOPA)-co-P(DMAEMA+), which resulted in the in-situ formation of silver based nanoparticles (Ag° and AgCl) that are sources of biocial Ag+. The layer-by-layer deposition of aqueous P(DOPA)-co-P(DMAEMA+)/AgCl/Ag0 suspension and aqueous solution of PSS provides stainless steel with high antibacterial activity against Gram-negative E. Coli bacteria. Moreover, after silver depletion, films retain some antimicrobial activity, thanks to the ammonium groups of the copolymer. We will also show how the antibacterial activity of the films can then be easily re-boosted. The multi-functionality of the P(DOPA)-co-P(DMAEMA+) is a key issue in this process (i) the DOPA co-units are anchored to stainless steel, (ii) these co-units reduce partly AgNO3 into Ag0 nanoparticles and stabilize them by chelation, (iii) the chloride counter-anions react with AgNO3 by ionic exchange, leading to the in situ formation of AgCl particles, and (iv) the ammonium groups are responsible for permanent antibacterial activity. Besides the advantage of the all-in-one process, another major advantage of the approach proposed here is the implementation of the whole process of film formation, including the synthesis of P(DOPA)-co-P(DMAEMA+), in aqueous media under very mild conditions. It makes the strategy very attractive for industrial scaling-up and sustainability applications. [less ▲]

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See detailNanocoatings of inorganic surfaces by molecular biomimetic
Vreuls, Christelle ULg; Genin, Alexis ULg; Zocchi, Germaine ULg et al

Poster (2010, March 22)

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See detailInorganic-binding peptides as tools for surface quality control
Vreuls, Christelle ULg; Zocchi, Germaine ULg; Genin, Alexis ULg et al

in Journal of Inorganic Biochemistry (2010)

This paper highlights an innovative application of inorganic-binding peptides as quality control tools for detecting defects on inorganic surfaces of any shape. The approach involves attaching a ... [more ▼]

This paper highlights an innovative application of inorganic-binding peptides as quality control tools for detecting defects on inorganic surfaces of any shape. The approach involves attaching a fluorescent label to an inorganic-binding peptide and exploiting the peptide's high binding specificity to detect, by simple fluorescence microscopy, chemical composition defects of microm size and crystallographic state defects. Proof of concept was demonstrated by monitoring binding of a previously isolated ZnO-binding peptide to galvanized steel substrates. The approach was further validated for TiO(2) coatings and stainless steel, with two new, specific inorganic-binding peptides isolated by phage display. [less ▲]

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See detailPrevention of bacterial biofilms by covalent immobilization of peptides onto plasma polymer functionalized substrates
Vreuls, Christelle ULg; Zocchi, Germaine ULg; Thierry, Benjamin et al

in Journal of Materials Chemistry (2010), 20

In this study, robust antibacterial coatings were created on stainless steel through the covalent grafting of antibacterial peptides onto an organic-polymeric interlayer deposited by RF-glow discharge ... [more ▼]

In this study, robust antibacterial coatings were created on stainless steel through the covalent grafting of antibacterial peptides onto an organic-polymeric interlayer deposited by RF-glow discharge plasma. X-Ray photoelectron spectroscopy was used to characterize and optimize the two steps of the coating process. The biocidal activity of these surfaces was demonstrated against both Gram+ and Gram- bacteria using ISO tests. 3 to 6 log10 reductions of both Gram+ and Gram- bacterial strains were obtained compared to uncoated stainless steel and depending on the particular antibacterial peptide immobilized. Importantly the antibacterial surfaces were resistant to several cleaning conditions. The latter is significant as the stability of such antibacterial surfaces in close to real life conditions is a major concern and leaching, de-lamination, rearrangement and ageing of the coating can lead to insufficient long term biofilm resistance of the surface. [less ▲]

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See detailBiomolecules in multilayer film for antimicrobial and easy-cleaning stainless steel surface applications
Vreuls, Christelle ULg; Zocchi, Germaine ULg; Garitte, Geoffrey ULg et al

in Biofouling (2010), 26(6), 645-656

Microorganisms are able to attach to, grow on, and ultimately form biofilms on a large variety of surfaces, such as industrial equipment, food contact surfaces, medical implants, prostheses and operating ... [more ▼]

Microorganisms are able to attach to, grow on, and ultimately form biofilms on a large variety of surfaces, such as industrial equipment, food contact surfaces, medical implants, prostheses and operating rooms. Once organized into biofilms, bacteria are difficult to remove and kill, which increases the risk of cross-contamination and infection. One way to address the problem may thus be to develop antibacterial, anti-adhesion, ‘easy cleaning’ surfaces. In this study, stainless steel (SS) surfaces with antibacterial properties were created by embedding several antimicrobial peptides in a multilayer film architecture. The biocidal effect of these surfaces was demonstrated against both Gram-positive and Gram-negative bacteria according to two ISO tests. Also, coating SS surfaces with either mucin or heparin led to a reduction of <i>S. epidermidis</i> adhesion of almost 95% <i>vs</i> the bare substratum. Finally, by combining both antibacterial and anti-adhesion biomolecules in the same multilayer film, SS surfaces with better cleanability were produced. This surface coating property may help to delay the buildup of a dead bacterial layer which is known to progressively reduce exposure of the coating, leading to an undesirable decrease in the antibacterial effect of the surface. [less ▲]

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See detailAll-in-one strategy for the fabrication of antimicrobial biomimetic films on stainless steel
Charlot, Aurélia; Sciannamea, Valérie; Lenoir, Sandrine et al

in Journal of Materials Chemistry (2009), 19

Here we report on an all-in-one approach to prepare robust antimicrobial films on stainless steel. The strategy is based on the layer-by-layer deposition of polyelectrolytes. A polycationic copolymer ... [more ▼]

Here we report on an all-in-one approach to prepare robust antimicrobial films on stainless steel. The strategy is based on the layer-by-layer deposition of polyelectrolytes. A polycationic copolymer bearing 3,4-dihydroxyphenylalanine units (DOPA, a major component of natural adhesives) was synthesized and co-deposited with precursors of silver nanoparticles as the first layer. The presence of DOPA units ensures a strong anchoring on the stainless steel substrate, and the silver nanoparticles are sources of biocidal Ag+, providing stainless steel with antimicrobial activity. We show that multilayered films, obtained by alternating this nanoparticle-loaded polycationic copolymer with polystyrene sulfonate, a commercial polyanion, results in stainless steel with high antibacterial activity against Gram-negative E. coli bacteria. The polycationic layers are a reservoir of Ag+ that can be reactivated after depletion. The whole process of film formation, including the synthesis of the copolymer, is conducted in aqueous media under very mild conditions, which makes it very attractive for industrial scale-up and sustainable applications. [less ▲]

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See detailNanocoatings of inorganic surfaces by the layer by layer (LbL) technology
Faure, Emilie ULg; Zocchi, Germaine ULg; Lenoir, Sandrine et al

Poster (2009, April 02)

Detailed reference viewed: 48 (17 ULg)