References of "Antoine, Nadine"
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See detailIn vitro culture of seal muscle-derived satellite cells
Freichels, Astrid ULg; Baise, Etienne ULg; Jauniaux, Thierry ULg et al

Poster (2014, April)

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See detailMechanoreceptors in the anterior horn of the equine medial meniscus: an immunohistochemical approach.
Nemery, Elodie ULg; Gabriel, Annick ULg; Piret, Joëlle ULg et al

Poster (2014)

Mechanoreceptors are “encapsulated sensory end-organs” involved in proprioceptive function. Given the high incidence of meniscal injuries in horses, the clinical interest in these mechanoreceptors ... [more ▼]

Mechanoreceptors are “encapsulated sensory end-organs” involved in proprioceptive function. Given the high incidence of meniscal injuries in horses, the clinical interest in these mechanoreceptors, particularly in the meniscus, and the lack of information concerning them in equine menisci, our objective was to study these corpuscles in the anterior horn of the equine medial meniscus, which is the most common localization reported for equine meniscal injuries. An immunohistochemical approach to detect Schwann cells and nerve fibres allowed us to localize and identify these corpuscles within the meniscus. Three types of mechanoreceptors were identified and localized between the abaxial quarter and the abaxial third of the meniscus: the Ruffini, Pacini and Golgi corpuscles. In conclusion, from a purely fundamental point of view, our work highlights for the first time the presence of MCR at the level of the anterior horn of the equine medial meniscus and proposes a classification based on specific immunocytochemical techniques. This morphological approach could serve as a basis for clinical studies, in order to evaluate the impact of these corpuscles on the poor sportive prognosis in equine meniscal tears. [less ▲]

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See detailIs 5-Hydroxytryptamine (serotonin) involved in the pathogenesis of idiopathic pulmonary fibrosis in dogs ?
Krafft, Emilie ULg; Roels, Elodie ULg; Heillilä, HP et al

in Proceedings of the 23th ECVIM Meeting (2013, September)

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See detailIs the CXC-Chemokine CXXL8 involved in the breed predisposition of west highland white terrier to canine idiopathic pulmonary fibrosis ?
Roels, Elodie ULg; Krafft, Emilie ULg; Laurila, HP et al

in Proceedings of the 23th ECVIM Meeting (2013, September)

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See detailThree-dimensional reconstruction of the pharyngeal tonsil innervation pattern in sheep
Toppets, Vinciane ULg; Piret, Joëlle ULg; Gabriel, Annick ULg et al

in Journal of Neuroimmunology (2013), (262), 79-84

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See detailMorphometric analyses of the normal suspensory ligament in Standardbreds
Shikh Al Sook, Mohamad Khir ULg; Antoine, Nadine ULg; Piret, Joëlle ULg et al

Poster (2013, July)

The suspensory ligament (SL) is composed of connective tissue (CT) with a variable proportion of muscle (MT) and adipose tissue (AT). The aim of our study is to quantify the CT, MT and AT within the SL in ... [more ▼]

The suspensory ligament (SL) is composed of connective tissue (CT) with a variable proportion of muscle (MT) and adipose tissue (AT). The aim of our study is to quantify the CT, MT and AT within the SL in sound horses. Right limbs from 11 horses were collected. Samples from 6 levels of the SL were embedded in paraffin or in Tissue-Tek®. Most of the paraffin sections were shredded. Using the cryosection, some artefacts appeared. Cryoprotection was carried out, which produced the best results. Hematoxylin–phloxine–saffron and Hematoxylin–eosin gave a good contrast of colours between the tissues allowing the use of an image analysis programme. The percentage of MT and AT decreased significantly (P < 0.0001), whereas the percentage of CT increased significantly (P < 0.0001) with age and when descending from the proximal to the distal level of the SL. The percentage of MT was significantly higher (P < 0.0001) in females than males, while the percentage of CT was significantly higher (P < 0.0001) in males than females. The percentage of AT was significantly higher (P = 0.0278) in pelvic limbs than in thoracic limbs. These results confirm the variation in tissue composition within the SL of sound horses. [less ▲]

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See detailPrevalence of naturally occurng cartilage defects in the ovine knee
Vandeweerd, Jean-Michel; Hontoir, Fanny; Kirschvink, Nathalie et al

in Osteoarthritis and cartilage (2013), 21

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See detailMorphometric analyses of the body and the branches of the normal third interosseous muscle (suspensory ligament) in Standardbreds
Shikh Al Sook, Mohamad Khir ULg; Antoine, Nadine ULg; Piret, Joëlle ULg et al

in Anatomia, Histologia, Embryologia (2013)

The third interosseous muscle (suspensory ligament, TIOM) is composed of connective tissue (CT) with a variable proportion of muscle (MT) and adipose tissue (AT). The aim of our study is to quantify the ... [more ▼]

The third interosseous muscle (suspensory ligament, TIOM) is composed of connective tissue (CT) with a variable proportion of muscle (MT) and adipose tissue (AT). The aim of our study is to quantify the CT, MT and AT within the body and the branches of right thoracic and pelvic limbs TIOM in sound horses to determine whether there are differences in CT, MT and AT between age, sex, limbs and levels. Right limbs from 11 sound horses were collected. Samples from 6 levels of the TIOM were embedded in paraffin or in Tissue-Tek®. Most of the paraffin sections were shredded. Using the cryosection, some artefacts appeared. Cryoprotection was carried out, which produced the best results. Hematoxylin–phloxine–saffron and Hematoxylin–eosin gave a good contrast of colours between the tissues observed allowing the use of an image analysis programme to calculate percentage of each tissue within the TIOM. The percentage of MT and AT decreased significantly (P < 0.0001), whereas the percentage of CT increased significantly (P < 0.0001) with age and when descending from the proximal to the distal level of the TIOM. The percentage of MT was significantly higher (P < 0.0001) in females than males, while the percentage of CT was significantly higher (P < 0.0001) in males than females. The percentage of AT was significantly higher (P = 0.0278) in pelvic limbs than in thoracic limbs. These results confirm the variation in tissue composition within the TIOM of sound horses. [less ▲]

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See detailFeline polymorphonuclear neutrophils produce pro-inflammatory cytokines following exposure to Microsporum canis
Cambier, Ludivine ULg; Mathy, A; Baldo, A et al

in Veterinary Microbiology (2013), 162(2-4), 800-805

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See detailDevelopment of Purkinje cells in the ovine brain
Salouci, Moustafa ULg; Engelen, Virginie; Gyan, Mathilda et al

in Anatomia, Histologia, Embryologia (2012), 41(3), 227232

Purkinje cells are involved in many vital functions within the body. Twenty ovine fetuses ranging from 2 to 5 months of gestation, two lambs in the first week after birth and three adult sheep were ... [more ▼]

Purkinje cells are involved in many vital functions within the body. Twenty ovine fetuses ranging from 2 to 5 months of gestation, two lambs in the first week after birth and three adult sheep were studied. Sections of the cerebellum were stained with haematoxylin and eosin, cresyl violet and Klu¨ver–Barrera. This study indicates that Purkinje cells began to appear after the 15th week of gestation. There were varying degrees of development of Purkinje cells in different zones of the cerebellum. Our findings in sheep fetuses suggest that the maturation of Purkinje cells starts in the caudal regions of the cerebellum and that the process begins in the vermis before it does in the cerebellar hemispheres. The alignment of Purkinje cells was found to be very regular in the caudal regions of the cerebellum. A partial absence of Purkinje cells in the rostral regions of the cerebellum was observed in both sheep fetuses and adult sheep. In the first post-natal week, some ectopic Purkinje cells were found in the white matter of the cerebellum. [less ▲]

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See detailLong term culture and characterization of chicken primordial germ cells
Tonus, Céline ULg; Waroux, Olivier ULg; Cloquette, Karine et al

Poster (2012, November)

Avian primordial germ cells (PGCs), can keep their germ cells properties and are foreseen as promising tools for developing avian transgenesis and preservation of genetic resources of endangered species ... [more ▼]

Avian primordial germ cells (PGCs), can keep their germ cells properties and are foreseen as promising tools for developing avian transgenesis and preservation of genetic resources of endangered species. We have developed original methods that allow long term (20 month) expansion of primary cultures of undifferentiated PGCs and their efficient cryopreservation. Blood samples were collected from stage 13-18 embryos, pooled, deposited in cell culture inserts and co-cultivated in the presence of irradiated BRL cells. This physically separated co-culture system along with selective culture medium promoted emergence, selection and proliferation of undifferentiated PGCs lines. Overall, 35% of blood samples gave rise to PGCs cell lines originating from three commercial layer breeds and two Belgian endangered breeds. PGCs lines were first characterised for the expression of the stem cells and PGCs characteristic marker SSEA-1 by FACS (expression rate: 90-99%). RT-PCR confirmed expression of germ-line specific markers (CVH, CDH, DAZL), pluripotency markers (cPouV, cSox2, cNanog), telomerase and CXCR4 receptor. In addition, by means of a quantitative PCR amplification of a chromosome W specific sequence, we demonstrated a drift of all our lines towards the male sex (WL), while they were initially isolated from pooled blood samples with statistically equivalent numbers of male and female embryos (35 females: 29 males). PGCs were subsequently efficiently cryopreserved by slow freezing or by a newly developed vitrification method. Labelled PGCs from 10 lines were injected in recipient embryos. Colonization of the genital ridges confirmed that PGCs retain their gonadal migratory ability, both after long-term culture (min 3, max 20 month) and after cryopreservation. [less ▲]

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See detailOPTIMIZATION OF HUMAN ES CELLS (hESCs) CRYOPRESERVATION
Connan, Delphine ULg; Ectors, Fabien ULg; Grobet, Luc ULg et al

Poster (2012, October 22)

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See detailThe three-dimensional reconstruction of the innervation pattern in the lymphoid compartment of the ovine pharyngeal tonsil highlighted a possible way of neuro-invasion by the scrapie agent.
Toppets, Vinciane ULg; Piret, Joëlle ULg; Grobet, Luc ULg et al

in Proceedings of the 2nd Scientific Meeting of the Faculty of Veterinary Medicine, ULg, Belgium, October 19, 2012 (2012, October 19)

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See detailMorphological and morphometric analyses of the suspensory ligament in Standardbreds
Shikh Al Sook, Mohamad Khir ULg; Antoine, Nadine ULg; Piret, Joëlle ULg et al

Poster (2012, October)

Ultrasound techniques allow examination of some parts of the suspensory ligament (SL) but "anomalies" are regularly observed. Their significance is not known. Few studies have described the relationship ... [more ▼]

Ultrasound techniques allow examination of some parts of the suspensory ligament (SL) but "anomalies" are regularly observed. Their significance is not known. Few studies have described the relationship between ultrasonographic appearance and the exact morphology in histological sections. The aim of this study is to develop good techniques for cutting, staining, and showing the variation in the tissue composition within the SL. The SLs from the right limbs of 11 horses were collected. Samples were taken from cross-sections at six levels of the SL and they were embedded in paraffin or in Tissue-Tek®. Most of the paraffin sections were shredded. By using the cryosection technique, some freezing artifacts (holes) appeared. Therefore, a technique of freezing with cryoprotection was carried out, which produced the best results. Hematoxylin-phloxine-saffron gives a good contrast of colors between the tissues observed allowing the use of an image analysis program. The percentage of each tissue within the SL for each section and for six levels of the ligament was calculated. Results were analyzed by SAS software. The muscle tissue (PMT) and adipose tissue (PAT) decreased significantly (p < 0.0001), whereas the connective tissue (PCT) increased significantly (p < 0.0001) with age and when descending from the proximal to the distal level of the SL. The PMT was significantly higher (p < 0.0001) in females than males, while the PCT was significantly higher (p < 0.0001) in males than females. The PAT was significantly higher (p = 0.0278) in hindlimbs than in forelimbs. [less ▲]

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