References of "Amory, Hélène"
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See detailTwo-dimensional speckle tracking echocardiography in calves: feasibility and repeatability study
Lecoq, Laureline ULiege; Moula, Nassim ULiege; Amory, Hélène ULiege et al

in Journal of Veterinary Cardiology : The Official Journal of the European Society of Veterinary Cardiology (2017)

Objectives: To assess the feasibility and repeatability of two-dimensional speckle tracking echocardiography for the evaluation of left ventricular function in healthy calves. Animals: 12 Holstein calves ... [more ▼]

Objectives: To assess the feasibility and repeatability of two-dimensional speckle tracking echocardiography for the evaluation of left ventricular function in healthy calves. Animals: 12 Holstein calves, 62 ± 11.6 days old; 75.25 ± 5.4 Kg. Methods: Observational study. Right parasternal short-axis views at papillary muscle level were recorded in standing calves and subsequently analysed by two-dimensional speckle tracking for global and regional radial and circumferential strains and strain rates and radial displacement. Echocardiographic examinations were performed by 2 observers to evaluate intra- and interobserver repeatability and variability. Results: Two-dimensional speckle tracking was feasible in all calves. Automated tracking was better in systole than in diastole. Repeatability of the technique was good in calves. Systolic radial strain and strain rate peak values showed little variability compared to systolic circumferential strain and strain rate and to all diastolic measurements. Variability of the interobserver measurements was greater than the intraobserver measurements. Conclusions: Two-dimensional speckle tracking is feasible in calves. As in other species, evaluation of systolic radial left ventricular function is more reliable than circumferential and diastolic left ventricular function. [less ▲]

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See detailAcylcarnitines profile best predicts survival in horses with atypical myopathy
BOEMER, François ULiege; Detilleux, Johann ULiege; CELLO, Christophe ULiege et al

in PLoS ONE (2017)

Equine atypical myopathy (AM) is caused by hypoglycin A intoxication and is characterized by a high fatality rate. Predictive estimation of survival in AM horses is necessary to prevent unnecessary ... [more ▼]

Equine atypical myopathy (AM) is caused by hypoglycin A intoxication and is characterized by a high fatality rate. Predictive estimation of survival in AM horses is necessary to prevent unnecessary suffering of animals that are unlikely to survive and to focus supportive therapy on horses with a possible favourable prognosis of survival. We hypothesized that outcome may be predicted early in the course of disease based on the assumption that the acylcarnitine profile reflects the derangement of muscle energetics. We developed a statistical model to prognosticate the risk of death of diseased animals and found that estimation of outcome may be drawn from three acylcarnitines (C2, C10:2 and C18 -carnitines) with a high sensitivity and specificity. The calculation of the prognosis of survival makes it possible to distinguish the horses that will survive from those that will die despite severe sign of acute rhabdomyolysis in both groups [less ▲]

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See detailReference values of two-dimensional and M-mode echocardiographic measurements as a function of body size in various equine breeds and in ponies.
Al-Haidar, Ali; Moula, Nassim ULiege; Leroux, Aurélia ULiege et al

in Journal of Veterinary Cardiology : The Official Journal of the European Society of Veterinary Cardiology (2017)

Introduction: The aim of this study was to establish echocardiographic reference values for the equine species using allometric regression equations based on body weight (BW) and thoracic circumference ... [more ▼]

Introduction: The aim of this study was to establish echocardiographic reference values for the equine species using allometric regression equations based on body weight (BW) and thoracic circumference (TC). Animals: A total of 239 horses or ponies were studied, including 65 warmbloods, 33 Standardbreds, 41 Thoroughbreds, 32 Arabian horses, 28 draft horses and 40 ponies aged from 1 day to 30 years, weighing from 18 to 890 kg, with no evidence of cardiac disease. Methods: For each horse or pony, a two-dimensional and M-mode echocardiography was performed. Within each breed, the relationships between BW or TC and echocardiographic dimensions were examined using power regression equations. Predictions and their 95% prediction intervals were calculated for the echocardiographic measurements. Results: Within each breed, all echocardiographic measurements showed a significant and positive relationship with a high coefficient of determination for the estimation of the regression equations using BW and TC as the main explanatory variables. Breed-specific power regression equations as well as the 95% prediction intervals were calculated for each echocardiographic measurement as a function of BW and TC. Conclusions: In the future, the body size-corrected and breed-specific echocardiographic reference values calculated in the present study could be used to discriminate between normal and abnormal values in a given animal. [less ▲]

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See detailDiagnostic value of serologic tests and seroprevalence of Borreliosis in horses living in southern Belgium
Cerri, Simona ULiege; Meersschaert, C; Houben, Rosa et al

Poster (2016, November 04)

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See detailLONG TERM PROGNOSIS OF MODERATE OR SEVERE LEFT-SIDED CARDIAC VALVULAR REGURGITATIONS IN HORSES
Leroux, Aurélia ULiege; Goudmaeker, Apolline; Fraipont, Audrey ULiege et al

in Proceedings of the 9th ECEIM Congress (2016, November)

Mitral and aortic regurgitations (MR and AR) are common in horses. Unlike mild valvular regurgitations, long-term prognosis of moderate or severe MR and AR is suspected to be guarded since they might ... [more ▼]

Mitral and aortic regurgitations (MR and AR) are common in horses. Unlike mild valvular regurgitations, long-term prognosis of moderate or severe MR and AR is suspected to be guarded since they might induce congestive heart failure (CHF). The aim of this study is to perform a long-term follow-up of horses with moderate or severe MR and/or AR to confirm this prognosis. Firstly medical files of horses with moderate or severe MR and/or AR that had a follow-up, were retrospectively reviewed over a 15-years period (2000-2015). Clinical and echocardiographic data of 25 horses with moderate or severe MR (16/25) or AR (8/25) or both (1/25) were considered. All horses had undergone 2 or more echocardiographic exams 6 months to 10 years apart. Eight horses developed CHF (4 MR and 4 AR) and all died/were euthanized. Diastolic left ventricular internal diameter, left atrial diameter and pulmonary artery diameter were/became above reference values in 17/25 horses but no significant difference was observed between the first and the follow-up measurements (Student's t-test, p<0.05). Secondly follow-up data of additional horses with moderate or severe MR was collected by telephone survey. Owners of 27 horses agreed to answer the survey 1 to 6 years after diagnosis. Ten horses with CHF had died, 3/27 had developed CHF, 10/27 had remained clinically stable and 4/27 had died of non-cardiac causes. Results of this study confirm that moderate or severe MR and AR carry a guarded to poor long-term prognosis as 40% (21/52) of the studied horses developed CHF. [less ▲]

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See detailSeroprevalence of Borrelia burgdorferi in horses in the southern part of Belgium: a “one health” driven study
Meersschaert, Carole; Cerri, Simona ULiege; Houben rosa, Rosa et al

Conference (2016, September 08)

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See detailL'échocardiographie en médecine équine: principe et interprétation des images.
Amory, Hélène ULiege

in Nouveau Praticien Vétérinaire Equine (2016), 11(39), 55-60

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See detailMitochondrial function is altered in horse atypical myopathy
Lemieux, Hélène; BOEMER, François ULiege; van Galen, Gaby et al

in Mitochondrion (2016), 30

Equine atypical myopathy in Europe is a fatal rhabdomyolysis syndrome that results from the ingestion of hypoglycin A contained in seeds and seedlings of Acer pseudoplatanus. The hallmark of atypical ... [more ▼]

Equine atypical myopathy in Europe is a fatal rhabdomyolysis syndrome that results from the ingestion of hypoglycin A contained in seeds and seedlings of Acer pseudoplatanus. The hallmark of atypical myopathy consists of a severe alteration in the energy metabolism including a severe impairment in muscle mitochondrial respiration that could contribute to its high death rate [less ▲]

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See detailFaecal microbiota characterisation of horses using 16 rdna barcoded pyrosequencing, and carriage rate of clostridium difficile at hospital admission
Rodriguez Diaz, Cristina ULiege; Taminiau, Bernard ULiege; Brévers, Bastien et al

in BMC Microbiology (2015), 15

Background The equine faecal microbiota is very complex and remains largely unknown, while interspecies interactions have an important contribution to animal health. Clostridium difficile has been ... [more ▼]

Background The equine faecal microbiota is very complex and remains largely unknown, while interspecies interactions have an important contribution to animal health. Clostridium difficile has been identified as an important cause of diarrhoea in horses. This study provides further information on the nature of the bacterial communities present in horses developing an episode of diarrhoea. The prevalence of C. difficile in hospitalised horses at the time of admission is also reported. Results Bacterial diversity of the gut microbiota in diarrhoea is lower than that in non-diarrhoeic horses in terms of species richness (p-value <0.002) and in population evenness (p-value: 0.02). Statistical differences for Actinobacillus, Porphyromonas, RC9 group, Roseburia and Ruminococcaceae were revealed. Fusobacteria was found in horses with diarrhoea but not in any of the horses with non-diarrheic faeces. In contrast, Akkermansia was among the three predominant taxa in all of the horses studied. The overall prevalence of C. difficile in the total samples of hospitalised horses at admission was 3.7 % (5/134), with five different PCR-ribotypes identified, including PCR-ribotype 014. Two colonised horses displayed a decreased bacterial species richness compared to the remaining subjects studied, which shared the same Bacteroides genus. However, none of the positive animals had diarrhoea at the moment of sampling. Conclusions The abundance of some taxa in the faecal microbiota of diarrhoeic horses can be a result of microbiome dysbiosis, and therefore a cause of intestinal disease, or some of these taxa may act as equine enteric pathogens. Clostridium difficile colonisation seems to be transient in all of the horses studied, without overgrowth to trigger infection. A large proportion of the sequences were unclassified, showing the complexity of horses’ faecal microbiota. [less ▲]

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See detailQuantification of hypoglycin A in serum using aTRAQ((R)) assay
BOEMER, François ULiege; DEBERG, Michelle ULiege; SCHOOS, Roland ULiege et al

in Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (2015), 997

BACKGROUND: Hypoglycin A has been recently identified has the causal agent of atypical myopathy (AM) in horses. Its identification and quantification in equine's biological fluids is thus a major concern ... [more ▼]

BACKGROUND: Hypoglycin A has been recently identified has the causal agent of atypical myopathy (AM) in horses. Its identification and quantification in equine's biological fluids is thus a major concern to confirm maple poisoning and to provide insight into the poorly understood mechanism of hypoglycin A intoxication. METHODS: Quantification of hypoglycin A has been achieved with the aTRAQ kit for amino acid analysis of physiological fluids (AB Sciex). Acquisition method on mass spectrometer has been updated to record the hypoglycin A specific MRM transition. RESULTS: Outlined accuracy profiles demonstrated very reliable data. A good linearity was observed from 0.09 to 50mumol/L and precision was very good with coefficient of variation below 8%. Fifty-five samples collected from 25 confirmed AM horses revealed significant hypoglycin A concentrations, while toxin was not found in serum of 8 control animals. CONCLUSIONS: The described aTRAQ variant method has been analytically and clinically validated. The reliability of our approach is thus demonstrated into the workup of atypical myopathy. [less ▲]

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See detailLes nouveaux outils de diagnostic et de pronostic de la myopathie atypique
Habyarimana, Jean Belt Adélite ULiege; BOEMER, François ULiege; Amory, Hélène ULiege et al

in Proceeding de la 41ème Journée de la Recherche équine (2015)

In equines, ingestion of hypoglycin A, a toxin produced in the seeds (samaras) of sycamore maple (Acer pseudoplatanus) tree alters the energetic metabolism of muscle cells and results in atypical myopathy ... [more ▼]

In equines, ingestion of hypoglycin A, a toxin produced in the seeds (samaras) of sycamore maple (Acer pseudoplatanus) tree alters the energetic metabolism of muscle cells and results in atypical myopathy (AM). This alterations leads to a characteristic biochemical profile of acylcarnitines (AC) that enables to confirm the diagnosis of AM. This study aims at validating a methodology for the dosage of hypoglycin A in vegetal extracts but also in blood. In addition, the biochemical profile in AC has been determined in AM cases (5 survivors and 13 deceased) and in 5 horses suffering from exercise-induced myopathy. The AC profiles of these horses have been compared to the one of healthy horses (n = 35). This study showed that hypoglycin A was present in seeds and spring seedlings of sycamore and also in blood of AM cases horses. In addition, the establishment of AC profile contributes to the diagnostic and helps to assess the prognosis of AM cases. [less ▲]

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See detailEVALUATION OF THE CARDIOMYOTOXIC EFFECTS OF DOXYCYCLINE OVERDOSE IN CALVES USING 2-DIMENSIONAL SPECKLE TRACKING.
Lecoq, Laureline ULiege; Leroux, Aurélia ULiege; Brihoum, Mounir et al

in Journal of Veterinary Internal Medicine (2015), 29(4), 1254

Doxycycline (DOXY) is associated with left ventricular (LV) dysfunction in calves in accidental overdose but not in experimental models when evaluated with classical and Doppler echocardiography. Two ... [more ▼]

Doxycycline (DOXY) is associated with left ventricular (LV) dysfunction in calves in accidental overdose but not in experimental models when evaluated with classical and Doppler echocardiography. Two-dimensional-speckle tracking (2DST) is used to evaluate LV dysfunction in numerous species but not in cattle. The aim of this study was to evaluate the cardiotoxic effects of an experimental overdose of DOXY using 2DST in calves. Ten healthy male Holstein calves. Group 1: 5 calves (mean age 58.0 +/- 16.3 days; mean body weight 72.2 +/- 13.0 kg) received 25 mg/kg of DOXY orally for 5 days. Group 2: 5 calves (mean age 56.4 +/- 15.7 days; mean body weight 73.4 +/- 7.0 kg) received a placebo. Electrocardiography (ECG) and 2DST echocardiography were performed at day 0 and day 8. ECG tracings were analysed for occurrence of arrhythmias. 2DST measurements included global and segmental, peak values for radial and circumferential strains (SR, SC), strain rates (SrR, SrC), rotation (Rot), rotation rates (RotR) and radial displacement (DR). All calves completed the study. ECG recordings were unremarkable in both groups. Heart rate was neither significantly different between groups nor before and after treatment. LV systolic function was affected in calves receiving an overdose of DOXY as shown by a significant decrease of segmental SR (P < 0.05), SC (P < 0.05) and DR (P < 0.05) in treated calves compared to the placebo group in several segments. The SrC in early diastole was also significantly decreased in 1 segment (<0.05). In calves, DOXY overdose induces a LV dysfunction in systole, and to a lesser extent, in diastole. A better comprehension of the pathophysiology involved in the DOXY overdose will help in the treatment of accidental cases. [less ▲]

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See detailTWO-DIMENSIONAL SPECKLE TRACKING ECHOCARDIOGRAPHY IN CALVES: FEASIBILITY, REPEATABILITY AND VARIABILITY STUDY
Lecoq, Laureline ULiege; Amory, Hélène ULiege; Leroux, Aurélia ULiege

in Journal of Veterinary Internal Medicine (2015), 29(4), 1255

Two-dimensional speckle tracking (2DST) is a non-invasive technique used in many species to evaluate global and regional left ventricular (LV) function; however it received little attention in the bovine ... [more ▼]

Two-dimensional speckle tracking (2DST) is a non-invasive technique used in many species to evaluate global and regional left ventricular (LV) function; however it received little attention in the bovine species. The aim of this study was to assess the feasibility and reliability of 2DST for the evaluation of circumferential and radial LV wall motions in calves. Fourteen Holstein black calves (age: 62 11.6 days; body weight: 75.25 5.4 kg) were used in this observational study. Right parasternal short axis views at the level of the papillary muscles were recorded and subsequently analysed by 2DST for global and regional radial and circumferential strains and strain rates, radial displacement, rotation and rotation rate. Echocardiographic examinations were performed in unsedated, standing calves by two different observers to evaluate intra- and interobserver repeatability and variability. 2DST was feasible in all calves but 2 were excluded from analyses (ventricular septum defect and resting heart rate above 120 bpm, respectively). Automated tracking was better in systole than in diastole. Intraobserver repeatability was good to moderate for most systolic global and segmental peak values. Systolic peak values for radial strain and strain rate were more repeatable than for circumferential strain, circumferential train rate and diastolic measurements. Variability of the interobserver measurements was greater than the intraobserver measurements. Two-dimensional speckle tracking is feasible in calves and as in other species systolic radial function can be more reliably evaluated than circumferential and diastolic function. [less ▲]

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See detailBorréliose: mythe ou réalité ?
Amory, Hélène ULiege; Houben, Rosa ULiege; Pitel, Pierre Hugues et al

Conference (2014, November 15)

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See detailCarriage and acquisition rates of Clostridium difficile in hospitalized horses, including molecular characterization, multilocus sequence typing and antimicrobial susceptibility of bacterial isolates
Rodriguez Diaz, Cristina ULiege; Taminiau, Bernard ULiege; Brévers, Bastien et al

in Veterinary Microbiology (2014), 172

lostridium difficile has been identified as a significant agent of diarrhoea and enterocolitis in both foals and adult horses. Hospitalization, antibiotic therapy or changes in diet may contribute to the ... [more ▼]

lostridium difficile has been identified as a significant agent of diarrhoea and enterocolitis in both foals and adult horses. Hospitalization, antibiotic therapy or changes in diet may contribute to the development of C. difficile infection. Horses admitted to a care unit are therefore at greater risk of being colonized. The aim of this study was to investigate the carriage of C. difficile in hospitalized horses and the possible influence of some risk factors in colonization. During a seven-month period, faecal samples and data relating the clinical history of horses admitted to a veterinary teaching hospital were collected. C. difficile isolates were characterized through toxin profiles, cytotoxicity activity, PCR-ribotyping, antimicrobial resistance and multilocus sequence typing (MLST). Ten isolates were obtained with a total of seven different PCR-ribotypes, including PCR-ribotype 014. Five of them were identified as toxinogenic. A high resistance to gentamicin, clindamycin and ceftiofur was found. MLST revealed four different sequencing types (ST), which included ST11, ST26, ST2 and ST15, and phylogenetic analysis showed that most of the isolates clustered in the same lineage. Clinical history suggests that horses frequently harbour toxigenic and non-toxigenic C. difficile and that in most cases they are colonized regardless of the reason for hospitalization; the development of diarrhoea is more unusual. [less ▲]

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See detailAtrial Natriuretic Peptide in cardiac horses
Leroux, Aurélia ULiege; Al-Haidar, Ali; Remy, Benoît ULiege et al

in Voorjaarsdagen Proceedings (2014, April 17)

Background: Natriuretic peptides are cardiac biomarkers that have been shown to have high potential for diagnosis, prognosis, and guidance of treatment in human and small animal cardiology. However, their ... [more ▼]

Background: Natriuretic peptides are cardiac biomarkers that have been shown to have high potential for diagnosis, prognosis, and guidance of treatment in human and small animal cardiology. However, their diagnostic and prognosis value in the equine species has only been studied in a limited number of cases. Objective: To investigate the plasma atrial natriuretic peptide concentration (ANPPl) in a large group of horses with various degrees of valvular regurgitation (VR) and heart failure (HF). Animals: Ninety-one horses, admitted at the equine teaching hospital of the University of Liege, and with no, mild, moderate or severe VR and presenting various stages of HF, according to human and canine grading system (A: no VR, B1: asymptomatic VR without cardiac remodelling, B2: asymptomatic VR with cardiac remodelling, C: symptomatic VR). Methods: All horses underwent clinical and Doppler echocardiographic examination, which allowed determining the presence, nature, and severity of VR, and the HF stage. The ANPPl was measured using a commercially available human RIA test. The mean values of body weight, age, ANPPl and each echocardiographic parameter were compared between horses in stage A, B1, B2 or C of HF and between horses with no, mild, moderate or severe VR, using a one-way ANOVA test. Then, correlations between ANPPl and each echocardiographic parameter were assessed using a Pearson’s product-moment analysis. Results: Horses with severe and moderate VR had significantly higher ANPPl than horses with mild or no VR. ANPPl was significantly higher in horses at stage B2 and C of HF than horses at stage A and B1. Moreover, ANPPl was significantly correlated with most of the measured echocardiographic variables. This correlation was especially strong with the left atrial diameter and its percentage of dilation. Conclusions and Clinical Importance: Those results suggest that ANPPl in horses suffering from VR could have a high diagnostic value of HF, especially in horses with tricuspid or mitral insufficiency and with enlargement or dysfunction of the atria. [less ▲]

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See detailPrognostic value of echocardiographic and Doppler parameters in horses admitted for colic complicated by systemic inflammatory response syndrome
Borde, L.; Amory, Hélène ULiege; Grulke, Sigrid ULiege et al

in Journal of Veterinary Emergency and Critical Care (2014), 24(3), 302-310

Objective: To assess the prognostic value of echocardiographic parameters of left ventricular (LV) function in horses with systemic inflammatory response syndrome (SIRS). Design: Prospective observational ... [more ▼]

Objective: To assess the prognostic value of echocardiographic parameters of left ventricular (LV) function in horses with systemic inflammatory response syndrome (SIRS). Design: Prospective observational study. Setting: Veterinary teaching hospital. Animals: Forty-one horses admitted for colic with clinical evidence of SIRS. Interventions: All horses underwent Doppler echocardiographic examination on admission. LV echocardiographic parameters, including pulsed-wave tissue Doppler imaging parameters, were compared between nonsurvivors (n = 29) and horses that survived to discharge (n = 12). Measurements and Main Results: With comparable heart rate and LV preload estimate, LV stroke volume index, the velocity time integral, deceleration time, ejection time of Doppler aortic flow, and peak early diastolic myocardial velocity were lower in the nonsurviving than in the surviving horses, while pre-ejection period to ejection time ratio (PEP/ET) of Doppler aortic flow and the peak early diastolic filling velocity to peak early diastolic myocardial velocity ratio (E/Em) were higher (P < 0.05). A cut-off value of 0.26 for PEP/ET predicted mortality with 100% sensitivity and 42% specificity (area under the receiver operating characteristic curve: 0.71), whereas a cut-off value of 2.67 for E/Em predicted mortality with 100% sensitivity and 83% specificity (area under the receiver operating characteristic curve: 0.89). Conclusions: Echocardiography may provide prognostic information in colic horses with clinical evidence of SIRS. Especially, PEP/ET and E/Em could be useful markers of systolic and diastolic dysfunction, respectively, to detect horses with a high risk of death requiring more intensive cardiovascular monitoring as it has been reported in human patients with septic shock. © Veterinary Emergency and Critical Care Society 2014. [less ▲]

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See detailPrognostic value of echocardiographic and Doppler parameters in horses admitted for colic complicated by systemic inflammatory response syndrome.
Borde; Amory, Hélène ULiege; Grulke, Sigrid ULiege et al

in Journal of Veterinary Emergency and Critical Care (2014)

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