Genome-wide association study of acute renal graft rejection.
; ; et al
in American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons (2016)
Acute renal rejection is a major risk factor for chronic allograft dysfunction and long-term graft loss. We performed a genome-wide association study to detect loci associated with biopsy-proven acute T ... [more ▼]
Acute renal rejection is a major risk factor for chronic allograft dysfunction and long-term graft loss. We performed a genome-wide association study to detect loci associated with biopsy-proven acute T cell-mediated rejection occurring in the first year after renal transplantation. In a discovery cohort of 4127 European renal allograft recipients transplanted in eight European centers, we used a DNA pooling approach to compare 275 cases and 503 controls, on Illumina 2.5 M arrays. In an independent replication cohort of 2765 patients transplanted in two European countries, we identified 313 cases and 531 controls, in whom we genotyped individually the most significant SNPs from the discovery cohort. In the discovery cohort, we found 5 candidate loci tagged by a number of contiguous SNPs (>5) that was never reached in iterative in silico permutations of our experimental data. In the replication cohort, two loci remained significantly associated with acute rejection in both univariate and multivariate analysis. One locus encompasses PTPRO, coding for a receptor-type tyrosine kinase essential for B cell receptor signalling. The other locus involves ciliary gene CCDC67, in line with the emerging concept of a shared building design between the immune synapse and the primary cilium. This article is protected by copyright. All rights reserved. [less ▲]Detailed reference viewed: 9 (1 ULg)
Evaluation d’un nouvel estimateur du Débit de Filtration Glomérulaire cinétique (KeGFR) pour prédire la survenue d’une insuffisance rénale aiguë après chirurgie cardiaque : étude AKInetic.
; ; et al
Poster (2015)Detailed reference viewed: 1 (0 ULg)
KDIGO Guidelines and Kidney Transplantation: Is the cystatin-C Based Recommendation relevant?
; ; CAVALIER, Etienne et al
in American Journal of Transplantation (2015), 15(8), 2211-4
The KDIGO guidelines propose a new approach to diagnose chronic kidney disease (CKD) based on estimated glomerular ®ltration rate (GFR). In patients with a GFR value comprised between 45 and 59 mL/ min/1 ... [more ▼]
The KDIGO guidelines propose a new approach to diagnose chronic kidney disease (CKD) based on estimated glomerular ®ltration rate (GFR). In patients with a GFR value comprised between 45 and 59 mL/ min/1.73m2 as estimated by the CKD-EPI creatinine equation (eGFRcreat), it is suggested to con®rm the diagnosis with a second estimation using the CKD-EPI cystatin C-based equations (eGFRcys/eGFRcreat-cys). We sought to determine whether this new diagnostic strategy might extend to kidney transplant recipients (KTR) and help to identify those with decreased GFR. In 670 KTR for whom a measured GFR was available, we simulated the detection of CKD using the two-steps approach recommended by the guidelines in comparison to the conventional approach relying on creatinine equation. One hundred forty-®ve patients with no albuminuria had eGFRcreat between 45 and 59 mL/ min/1.73m2. Among them, 23% had inulin clearance over 60 mL/min/1.73m2 and were thus incorrectly classi®ed as CKD patients. When applying the Kidney Disease: Improving Global Outcomes (KDIGO) strategy, 138 patients were con®rmed as having a GFR below 60 mL/min with eGFRcreat-cys. However, 21% of them were misclassi®ed in reference to measured GFR. Our data do no not support the use of cystatin C as a con®rmatory test of stage 3A CKD in KTR. [less ▲]Detailed reference viewed: 32 (4 ULg)
MDRD versus CKD-EPI equation to estimate glomerular filtration rate in kidney transplant recipients
; ; et al
Poster (2013)Detailed reference viewed: 10 (0 ULg)
Nouvelles recommendations K/DIGO et transplantation rénale : quelle place pour la cystatine C ?
; ; et al
Conference (2013)Detailed reference viewed: 3 (0 ULg)
New K/DIGO guidelines and kidney transplantation: is the cystatin C-based recommendation relevant?
; ; et al
Conference (2013)Detailed reference viewed: 9 (0 ULg)
Comparison of acid and enzymatic methods for insulin dosage: Analytical performances and impact on glomerular filtration rate evaluation
DELANAYE, Pierre ; ; et al
in Clinica Chimica Acta (2012), 413(5-6), 556-560
Among issues susceptible to hamper a reliable measurement of inulin clearance, those regarding the dosage of inulin are largely neglected. We have compared the analytical performances of 2 commonly used ... [more ▼]
Among issues susceptible to hamper a reliable measurement of inulin clearance, those regarding the dosage of inulin are largely neglected. We have compared the analytical performances of 2 commonly used methods of inulin dosage (one “acid” and one “enzymatic” method) and studied their potential impact on the glomerular filtration rate (GFR) value given by inulin clearance. Repeatability, uncertainty and the beta-expectation limits were evaluated from pre-determined serum and urine pools of inulin. Agreement between the two methods was analyzed from 99 inulin clearances performed in renal transplant patients. Impact of the method of dosage on GFR evaluation was simulated according to the respective beta-expectations limits of each method. Overall, intra-assay coefficient of variability and relative bias were inferior to 5% and 10% for both methods. Contrary to the acid method, analytical performance of the enzymatic method was not influenced by the presence of glucose. The relative difference in GFR values obtained with the two methods in transplant patients was − 0.4 ± 10%. Simulations suggested that changes in inulin concentration attributable to analytical error could modify the value of GFR from − 12% to + 28%. In conclusion, while analytical performances are globally acceptable for both methods, they are not strictly equivalent. The impact on the determination of GFR, albeit limited, is not negligible and adds to other sources of inaccuracy. International standardization for the dosage of inulin is necessary. [less ▲]Detailed reference viewed: 25 (0 ULg)
Performance, limitations and utility of cystatin C as an endogenous GFR marker in renal transplantation
; ; et al
Poster (2012)Detailed reference viewed: 1 (0 ULg)
Comparaison d'une méthode "acide" et enzymatique pour le dosage de l'inuline
; Delanaye, Pierre ; et al
Poster (2010, September)Detailed reference viewed: 27 (3 ULg)
Donor-Estimated Glomerular Filtration Rate as a Criterion for Allocation of Expended Criteria Donors Kidney into Single or Dual Transplantation : The Choice of the Estimating Equation Matters
Delanaye, Pierre ; ; Rozet, Eric et al
Poster (2010, April)Detailed reference viewed: 18 (8 ULg)