Showing results 41 to 45 of 45
 Preparative enzymatic synthesis and in situ regeneration of nucleotide sugars with sucrose synthaseZervosen, Astrid  ; ; Conference (1995, July) Detailed reference viewed: 2 (1 ULg)Praeparative enzymatische Synthese von Nucleotidzuckern mit Saccharose Synthase aus ReisZervosen, Astrid ; Poster (1995, May 30) Detailed reference viewed: 3 (0 ULg) Application of sucrose synthase from rice grains for the synthesis of carbohydrates.; ; Zervosen, Astrid et alin Annals of the New York Academy of Sciences (1995), 750 The enzymatic synthesis of oligosaccharides offers an efficient way to obtain regiospecific and stereo-selective natural and nonnatural target molecules. However, the very expensive activated sugars are ... [more ▼] The enzymatic synthesis of oligosaccharides offers an efficient way to obtain regiospecific and stereo-selective natural and nonnatural target molecules. However, the very expensive activated sugars are required as substrates for glycosyltransferases. Activated sugars are usually synthesized by pyrophosphorylases from nucleoside triphosphates and sugar-l-phosphates.'.* For cyclic in situ regeneration, at least three enzymes are needed, which makes the synthesis of, for example, N-acetyllactosamine very complex by established procedure. We report here on the enzymatic synthesis of saccharides and activated sugars by the plant glycosyltransferase sucrose synthase (EC 2.4.1.13)! The glycosyltransferase has the unique feature of catalyzing in vitro the synthesis and cleavage of sucrose (SCHEME1 ). The main advantage of sucrose synthase over pyrophosphorylases is the utilization of nucleoside diphosphates (NDP) instead of nucleoside triphosphates to generate activated sugars. [less ▲] Detailed reference viewed: 26 (5 ULg)ISOLATION OF SUCROSE SYNTHASE FROM RICE (ORYZA-SATIVA) GRAINS IN PILOT-SCALE FOR APPLICATION IN CARBOHYDRATE SYNTHESIS; ; et al in Biotechnology & Applied Biochemistry (1995), 21(Part 1), 29-37 The application of sucrose synthase as a valuable biocatalyst for the synthesis of activated sugars and saccharides requires large amounts of enzyme. The purification of sucrose synthase starting from the ... [more ▼] The application of sucrose synthase as a valuable biocatalyst for the synthesis of activated sugars and saccharides requires large amounts of enzyme. The purification of sucrose synthase starting from the 9 kg scale of rice (Oryza sativa) grains was achieved in five steps with 11.3% yield and 192-fold purification. The final sucrose synthase preparation was free of nucleotide di- and monophosphatases, but did contain 0.05% of both invertase and UDP-glucose-hydrolysing activity. It can be efficiently utilized for the synthesis of activated sugars. Its application for the synthesis of saccharides is possible taking into account that 15% UDP-glucose is hydrolysed within 15 h incubation time [less ▲] Detailed reference viewed: 43 (7 ULg)CONTINUOUS ENZYMATIC-SYNTHESIS OF 2'-DEOXYTHYMIDINE-5'(ALPHA-D-GLUCOPYRANOSYL)DIPHOSPHATEZervosen, Astrid ; ; in Angewandte Chemie (International ed. in English) (1994), 33(5), 571-572 The preparation of primary nucleotide sugars like dTDP-glucose with sucrose synthase, a glycosyl transferase in plants, provides an alternative in enzymatic synthesis to the use of highly specific ... [more ▼] The preparation of primary nucleotide sugars like dTDP-glucose with sucrose synthase, a glycosyl transferase in plants, provides an alternative in enzymatic synthesis to the use of highly specific pyrophosphorylases. In an enzyme membrane reactor a continuous synthesis can be performed on preparative scale. [less ▲] Detailed reference viewed: 14 (5 ULg) |
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