References of "Wathelet, Bernard"
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See detailMultifacedted phenotype of a modified overproducing Bacillus subtilis strain
Leclere, V.; Bechet, M.; Adam, A. et al

Poster (2005)

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See detailImprovement of lupin seed valorisation by the pig with the addition of alpha-galactosidase in the feed and the choice of a suited variety.
Froidmont, E.; Wathelet, Bernard ULg; Beckers, Yves ULg et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2005), 9(4), 225-235

Three experiments were conducted to assess whether an a-galactosidase supplement improved the utilisation of lupin seeds by growing and finishing pigs and to determine the best lupin variety in presence ... [more ▼]

Three experiments were conducted to assess whether an a-galactosidase supplement improved the utilisation of lupin seeds by growing and finishing pigs and to determine the best lupin variety in presence of a-galactosidase. In experiment 1, three iso-nitrogen (iso-N) and iso-net energy (iso-NE) diets containing 15% of soybean meal (SBM diet) or 20% of lupin seeds (Lupinus albus var. Arès), with or without an a-galactosidase supplement (‘lupin’ and ‘lupin + a-gal’ diets, respectively), were ingested by 12 pigs fitted with a caecal canula, following a completely randomized experimental design. The ileal digestibility of a-galactosides tended to increase with the lupin + a-gal diet. Nitrogen retention of pigs eating the SBM diet was similar to that of those fed with the lupin + a-gal diet and higher than that observed for pigs given the lupin diet. The results suggested that a-galactosides are the main anti-nutritional factors in lupin seeds for pigs. The same diets were investigated in a production trial (experiment 2) involving 48 pigs with a live weight of between 30 and 105 kg. The fattening period was similar for the SBM and lupin + a-gal diets (107 d) and longer for the lupin diet (123 d). With the lupin diet there was a lower average daily gain (ADG, -16%) and a higher food conversion ratio (FCR, + 8%) than with the two other diets. The backfat of pigs fed with lupin-based diets contained fewer polyunsaturated fatty acids (PUFA) and had a lower w6/w3 ratio. In experiment 3, three iso-N and iso-NE diets containing different varieties of lupin (Lupinus albus cv Lublanc and Amiga; Lupinus angustifolius cv Boltensia) and an a-galactosidase supplement were investigated in 12 pigs fitted with a caecal canula, as in Exp. 1. The results showed that the digestible protein supply by the Boltensia diet was higher than in the others. However, the Boltensia variety led to a higher urinary N excretion than the Lublanc variety. The results obtained with Amiga were intermediate. Consequently, the utilisation of ingested and digested proteins was relatively high with the Lublanc diet, intermediate with the Amiga diet and low with the Boltensia diet. The results suggested that the Lublanc variety was better adapted for pig feed that contained an a-galactosidase supplement. [less ▲]

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See detailHemolytic activity of new linear surfactin analogs in relation to their physico-chemical properties
Dufour, Samuel; Deleu, Magali ULg; Nott, Katherine ULg et al

in Biochimica et Biophysica Acta - General Subjects (2005), 1726

New linear analogs of surfactin have been synthesized. Their physico-chemical parameters were determined. The results indicate that these linear products show surface activities although they are lowered ... [more ▼]

New linear analogs of surfactin have been synthesized. Their physico-chemical parameters were determined. The results indicate that these linear products show surface activities although they are lowered compared to those of cyclic compounds. The hemolytic activities have also been assayed. In contrast with cyclic surfactins, no significant hemolysis occurs for the linear products in the range of concentrations tested. Moreover, a protective effect against Triton X-100 induced hemolysis has been highlighted for linear surfactins. The concentration at which this protective effect happens is correlated directly to the CMC, and inversely to the acyl chain length of the product. In a hypotonic medium, analogs having a long acyl chain tend to increase the hemolysis, meanwhile the product with the shortest chain tends to decrease it. [less ▲]

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See detailHemolytic activity of new linear surfactin analogs
Dufour, Samuel; Deleu, Magali ULg; Wathelet, Bernard ULg et al

Poster (2005)

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See detailA Chemoselective Ligation For The Synthesis Of Amino Acid Derivatives Of Virginiamycin M-1
Nott, Katherine ULg; Dufour, Samuel; Gossele, Francis et al

in Tetrahedron Letters (2005), 46(43),

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See detailStudy of synthesis and surface activity of inulin lauric esters.
Ius, E.; Blecker, Christophe ULg; Verardo, G. et al

Poster (2004, February 11)

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See detailNew Methode for a Two-Step Hydrolysis and Chromatographic Analysis of Pectin Neutral Sugar Chains
Haikel, Garna; Mabon, Nicolas ULg; Wathelet, Bernard ULg et al

in Journal of Agricultural and Food Chemistry (2004), 52

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See detailNew method for a two-step hydrolysis and chromatographic analysis of pectin neutral sugar chains
Garna, H.; Mabon, N.; Wathelet, Bernard ULg et al

in Journal of Agricultural and Food Chemistry (2004), 52(15), 4652-4659

A new method for the determination of the main neutral sugars in pectin has been developed. The sample preparation involves a mild chemical attack followed by an enzymatic hydrolysis. The completeness and ... [more ▼]

A new method for the determination of the main neutral sugars in pectin has been developed. The sample preparation involves a mild chemical attack followed by an enzymatic hydrolysis. The completeness and nondestructive character of the method are demonstrated by comparison of the results obtained with different acids such as H2SO4, HCI, and trifluoroacetic acid (TFA) at different concentrations (2, 1, or 0.2 M) at two temperatures (80 or 100 degreesC). The chemical hydrolysis of pectin neutral sugar chains with strong acid (1 or 2 M) and high temperature (100 degreesC) shows that the liberation of the pectin sugars is not realized at the same rate for each sugar. Different optimum conditions are thus obtained. However, the chemical pectin hydrolysis with 0.2 M TFA at 80 degreesC is characterized by the liberation of pectin neutral sugar side chains without any degradation within 72 h of hydrolysis. Under these conditions, the liberation of some pectin sugars, essentially galactose, glucose, and rhamnose, was not complete. An enzymatic hydrolysis is necessary to obtain a complete release of all the sugars. The combination of the two treatments, a chemical hydrolysis realized with diluted acid (0.2 M) for 72 h at low temperature (80 degreesC) on one hand and an enzymatic hydrolysis on the other hand, allow a total liberation of pectin sugars. The quantitative analysis of the carbohydrates is realized with accuracy, high selectivity, and sensitivity with high-performance anion-exchange chromatography with pulsed-amperometric detection. The sugars can be analyzed without any derivatization with a limit of quantification of 0.1 mM. [less ▲]

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See detailThe Pyoverdins Of Pseudomonas Syringae And Pseudomonas Cichorii
Bultreys, A.; Gheysen, I.; Wathelet, Bernard ULg et al

in Zeitschrift Fur Naturforschung C-A Journal of Biosciences (2004), 59(9-10),

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See detailEnfin "Tubambe" devoile son identite! Hadraphe ethiopica (Bethune-Baker) (Limacodidae), une chenille comestible des forets claires zambeziennes.
Malaisse, François ULg; Demesmaecker, Anne; Matera, Joseph et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2003), 7(2), 67-77

La consommation humaine de chenilles de Limacodidae avait été signalée en Afrique centro-australe. L’une de ces espèces, Hadraphe ethiopica, a été déterminée. Des données relatives à sa biologie ont été ... [more ▼]

La consommation humaine de chenilles de Limacodidae avait été signalée en Afrique centro-australe. L’une de ces espèces, Hadraphe ethiopica, a été déterminée. Des données relatives à sa biologie ont été rassemblées. Des analyses d’échantillons séchés ont permis de préciser leur composition chimique et leur valeur nutritionnelle, notamment sur le plan des acides aminés et des acides gras. [less ▲]

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See detailWild edible mushrooms from a Zambezian woodland area (Copperbelt Province, Zambia).
Bourdeaux, Quentin; Buyck, B.; Malaisse, François ULg et al

in Geo-Eco-Trop (2003), 27(1-2), 33-44

Dans de nombreux pays d'Afrique tropicale, la consommation de champignons sauvages constitue un appoint alimentaire non négligeable pour les populations locales. La présente étude fut menée dans la ... [more ▼]

Dans de nombreux pays d'Afrique tropicale, la consommation de champignons sauvages constitue un appoint alimentaire non négligeable pour les populations locales. La présente étude fut menée dans la Copperbelt Province( Zambie), un territoire situé en Région zambézienne. La récolte de sporophores et les enquêtes participatives menées auprès des villageois ont permis de dresser une liste de 47 tâxons de champignons comestibles. [less ▲]

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See detailInfluence de la substitution du tourteau de soja par des graines de lupin, avec ou sans complément d'alpha-galactosidase, sur la digestibilité des régimes et la rétention azotée du porc en croissance
Froidmont, Eric; Schoeling, Olivia; Deliège, Françoise et al

in Journées Recherche Porcine (2003)

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See detailHigh-Performance Liquid Chromatography Analyses Of Pyoverdin Siderophores Differentiate Among Phytopathogenic Fluorescent Pseudomonas Species
Bultreys, Alain; Gheysen, Isabelle; Wathelet, Bernard ULg et al

in Applied and Environmental Microbiology (2003), 69(2),

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See detailCombined enzymatic hydrolysis and HPAEC method for simultaneous analysis of galacturonic acid and neutral sugars of pectin.
Garna, H.; Mabon, N.; Wathelet, Bernard ULg et al

in Communications in Agricultural and Applied Biological Sciences (2003), 68(2 Pt A), 297-300

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See detailNouvelles fractions d'inuline. Leur préparation et leur utilisation.
Blecker, Christophe ULg; Razafindralambo, Hary ULg; Chevalier, J. P. et al

Patent (2002)

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See detailCaractérisation physico-chimique de gommes végétales exsudées par quelques acacias du Burkina Faso
Djiré, O.; Ntema, P.; Zgoulli, S. et al

in Science et Technique (2002), 25(2), 37-47

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See detailFast And Reliable Chromatographic Procedure For The Purification Of Virginiamycin M-1 Factor
Nott, Katherine ULg; Paquot, Michel ULg; Heilporn, Sylvie et al

in Chromatographia (2002), 56(5-6),

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See detailEthno-Ecological approach of Tibet south-central rural environment knowledge as a support to Kashin-Beck disease's etiology
Malaisse, François ULg; Begaux, Françoise; Chasseur, Camille et al

in Bulletin des Séances de l'Académie Royale des Sciences d'Outre-Mer (2002), 48

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See detail(EN) NOVEL INULIN FRACTIONS, PREPARATION AND USE
Blecker, Christophe ULg; Razafindralambo, Hary ULg; CHEVALIER, J. P. et al

Patent (2001)

The invention concerns novel inulin fractions, characterised by endothermal peaks of differential calorimetric analysis and by domains of polymerising degree, methods for obtaining said fractions by ... [more ▼]

The invention concerns novel inulin fractions, characterised by endothermal peaks of differential calorimetric analysis and by domains of polymerising degree, methods for obtaining said fractions by fractionating solutions, solutions saturated or dispersed with inulins, hydrocolloidal compositions and gels obtained with said fractions, and uses of said fractions in food composition or the like. [less ▲]

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