References of "Waltregny, David"
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See detailIdentification of accessible proteins expressed in human breast cancer
Castronovo, Vincenzo ULg; Kischel, Philippe; Guillonneau, François et al

Scientific conference (2006)

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See detailRegulation by androgens of EGF receptor family members in prostate cancer cells
Pignon, Jean-Christophe; Delacroix, Laurence ULg; Waltregny, David ULg et al

in Proceedings of the American Association for Cancer Research (2006)

After an initial positive response to anti-androgen treatment, prostate cancer (PCa) cells usually become hormone-refractory in spite of their persistent expression of the androgen receptor (AR ... [more ▼]

After an initial positive response to anti-androgen treatment, prostate cancer (PCa) cells usually become hormone-refractory in spite of their persistent expression of the androgen receptor (AR). Overexpression of tyrosine kinase receptors in androgen-deprived PCa cells, such as those of the EGF receptor (EGFR) family, may be responsible for AR activation and growth of androgen-deprived tumours. Our goal is to understand the control of the expression of the EGFR family members by androgens in PCa. Hormone response was compared in hormone-sensitive LNCaP and hormone-insensitive DU145 PCa cell lines. These cells do not express ErbB4. EGFR, erbB2 and erbB3 protein half-life is much longer in DU145 than in LNCaP cells grown in complete medium. Dihydrotestosterone (DHT) modulates EGFR and erbB2 transcript and protein levels only in LNCaP cells. ErbB3 is not an androgen-responsive gene. EGFR mRNA and protein levels are increased while erbB2 mRNA and protein levels are decreased after DHT treatment of cells cultured in steroid-deprived medium. ErbB2 mRNA and protein levels are increased in LNCaP cells following DHT withdrawal. In order to understand the mechanisms by which androgens control the expression of EGFR and ERBB2 genes, half lifes of the corresponding mRNAs and proteins were compared in cells grown in presence or absence of DHT. The effect of DHT on EGFR gene expression is complex. Indeed, DHT stabilizes the protein. Moreover, a superinduction of EGFR mRNA was observed in cells treated with cycloheximide (CHX) before addition of the hormone, suggesting an effect on transcript stability. In contrast, erbB2 mRNA and protein stability was not affected by DHT. CHX treatment for 2h before addition of DHT suppresses the androgen-induced down-regulation of erbB2 mRNA levels. In summary, androgen-mediated regulation of EGFR and ERBB2 genes expression is complex. DHT influences EGFR gene transcription, mRNA and protein stability. DHT does not affect erbB2 mRNA and protein stability but acts indirectly on transcription. Current experiments are undertaken to verify these observations by Chromatin-IP experiments on both genes promoters. [less ▲]

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See detailHistone deacetylase 7 is involved in the control of angiogenesis by regulating platelet-derived growth factor-β
Mottet, Denis; Bellahcene, Akeila ULg; Deroanne, Christophe et al

Conference (2006)

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See detailRole of tumor-associated lipogenesis in the formation and composition of membrane microdomains
Timmermans, L.; Kischel, Philippe; Beckers, Albert ULg et al

Conference (2006)

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See detailRole of histone deacetylases in myofibroblastic differenciation
Glénisson, Wendy; Waltregny, David ULg; Castronovo, Vincenzo ULg

Conference (2006)

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See detailAndrogens regulation of the ERBB1 and ERBB2 oncogene expression in human prostate cancer cells
Pignon, Jean-Christophe ULg; Delacroix, Laurence; Waltregny, David ULg et al

in Acta Clinica Belgica (2006), 61(2, MAR-APR), 95-95

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See detailExamination of the non-palpable cryptorchid testis
Waltregny, David ULg

Conference (2005, June)

La cryptorchidie affecre environ 1 garçon sur 150 et dans près de 20% des cas, la gonade n'est pas palpée. Lorsqu'il est présent, le testicule non palpable est situé entre l'orifice externe du canal ... [more ▼]

La cryptorchidie affecre environ 1 garçon sur 150 et dans près de 20% des cas, la gonade n'est pas palpée. Lorsqu'il est présent, le testicule non palpable est situé entre l'orifice externe du canal inguinal et le pôle inférieur du rein. Souvent cependant, lors de l'exploration chirurgicale, on constate l'existence de reliquats tissulaires à l'extrémité de vaisseaux spermatiques borgnes sans que l'on puisse reconnaître à proprement parler un testicule: on parle alors de testicule absent. Dans d'autres cas, on ne trouve pas de trace de vaisseau spermatique, de structure épidydimo-déférentielle ou encore de tissu testiculaire; il s'agit d'agénésie gonadique. Le testicule non palpé présente un risque accru d'infertilité, de transformation maliine, de hernies et malformations épididymo-déférentielles associées. Le traitement chirurgical est souvent plus complexe et les échecs plus fréquents. De nombreuses techniques d'imagerie ont été proposées comme modalités diagnostiques pour localiser le/les testicule(s) non palpé(s) (pneumopéritonéographie, angiographie, ultrasonographie, tomodensitométrie computérisée, RMN) mais aucune d'elles, à ce jour, n'est uniformément acceptée en raison du manque de fiabilité à détecter le testicule recherché et/ou des dangers potentiels résultant de leur emploi. Depuis la description par Cortesi en 1976 de l'utilisation de la cælioscopie pour la localisation du testicule impalpable, un intérêt croissant a été porté par les urologues et les chirurgiens pédiatriques à cette technique qui aujourd'hui joue un rôle central dans l'exploration du testicule cryptorchide non palpé. Les auteurs tentent d'établir une méthodologie algorythmique d'investigation des testicules cryptorchides non palpés à la lumière des données anatomiques, pathologiques et chirurgicales récentes de la littérature. [less ▲]

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See detailExpression and nuclear location of the transcriptional repressor kaiso is regulated by the tumor microenvironment
Soubry, Adelheid; van Hengel, Jolanda; Parthoens, Eef et al

in Cancer Research (2005), 65(6), 2224-2233

Kaiso is a BTB/POZ zinc finger protein originally described as an interaction partner of p120ctn. In cultured cell lines, Kaiso is found almost exclusively in the nucleus, where it generally acts as a ... [more ▼]

Kaiso is a BTB/POZ zinc finger protein originally described as an interaction partner of p120ctn. In cultured cell lines, Kaiso is found almost exclusively in the nucleus, where it generally acts as a transcriptional repressor. Here, we describe the first in situ immunolocalization studies of Kaiso expression in normal and cancerous tissues. Surprisingly, we found striking differences between its behavior in monolayers of different cell lines, three-dimensional cell culture systems, and in vivo. Although nuclear localization was sometimes observed in tissues, Kaiso was more often found in the cytoplasm, and in some cell types it was absent. In general, Kaiso and p120ctn did not colocalize in the nucleus. To examine this phenomenon more carefully, tumor cells exhibiting strong nuclear Kaiso staining in vitro were injected into nude mice and grown as xenografts. The latter showed a progressive translocation of Kaiso towards the cytoplasm over time, and even complete loss of expression, especially in the center of the tumor nodules. When xenografted tumors were returned to cell culture, Kaiso was re-expressed and was once again found in the nucleus. Translocation of Kaiso to the cytoplasm and down-regulation of its levels were also observed under particular experimental conditions in vitro, such as formation of spheroids and acini. These data strongly imply an unexpected influence of the microenvironment on Kaiso expression and localization. As transcriptional repression is a nuclear event, this phenomenon is likely a crucial factor in the regulation of Kaiso function. [less ▲]

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See detailLipomatose pelvienne associee a une cystite glandulaire: rapport de deux cas
Leruth, Elisabeth; Coppens, Luc ULg; Andrianne, Robert ULg et al

in Progrès en Urologie (2005), 15(1), 81-4

The authors report two cases of pelvic lipomatosis associated with glandular cystitis. This is a rare disease with variable and nonspecific clinical features. Complementary investigations useful for the ... [more ▼]

The authors report two cases of pelvic lipomatosis associated with glandular cystitis. This is a rare disease with variable and nonspecific clinical features. Complementary investigations useful for the diagnosis of pelvic lipomatosis are CT and especially MRI, intravenous urography and biopsies. The clinical features, radiological findings and therapeutic approach are discussed in the light of a review of the literature. [less ▲]

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See detailInside-out Transobturator Vaginal Tape (TVT-O): one-year results of a prospective study
Waltregny, David ULg; Reul, Olivier ULg; Keppenne, Véronique ULg et al

in European Urology Supplements (2005), 4(3), 1653

Title Inside-out transobturator vaginal tape (TVT-O): One-year results of a prospective study Introduction and Objectives The aim of this study was to prospectively assess the efficacy of a new surgical ... [more ▼]

Title Inside-out transobturator vaginal tape (TVT-O): One-year results of a prospective study Introduction and Objectives The aim of this study was to prospectively assess the efficacy of a new surgical technique, the inside-out transobturator vaginal tape (TVT-O), for the treatment of female stress urinary incontinence (SUI). Study design, materials and methods From 03/2003 through 10/2003, a TVT-O tape was inserted in 83 consecutive patients with clinical evidence of SUI. Preoperative evaluation included complete history, physical examination, urodynamics, urine analysis, and cystoscopy. None of the patients presented the following exclusion criteria: post-void residual (PVR) >100 cc, detrusor overactivity or acontractility, pregnancy, neurological pathology, active urinary or vaginal infection, age >85 years, negative stress test, and maximum cystometric capacity <300 mL. Post-operative evaluation was carried out using symptom scoring and quality of life (QoL) questionnaires, visual analog scales, physical examination, uroflowmetry, and PVR measurement. Cure was defined as no leakage based on both symptom scale scoring and physical examination. Improvement was defined as ≥50% decrease in symptoms based on the questionnaire’s results. Results Mean age of the patients was 61 years. The TVT-O procedure was associated with pelvic organ prolapse cure in 15 patients (18%). Follow-up time was ≥12 months in all women (mean = 13.6); 3 patients were lost to follow-up. No significant blood loss (≥100 cc), vaginal wall, urethral, or bladder perforation was encountered. No hematoma, vaginal or urethral erosion, or neurological complication was observed. No patient complained of persistent pain. At the latest follow-up visit, max flow rate was ≥10 mL/sec and PVR was <100 cc in 90% and 94% patients, respectively. Two patients underwent an immediate tape release procedure while the tape was sectioned in 2 other patients for retention and/or urgency associated with obstruction. Sixty eight patients (85%) were cured of their SUI while 9 patients (11%) were improved. Urgency questionnaire’s results showed that 5 (5/46) patients developed de novo urgency. Twenty and 14 out of the 34 patients with preoperative urge symptoms reported either disappearance or no change of urgency, respectively. Obstruction symptoms appeared or worsened in 3 patients and were unchanged or decreased in all other patients. Analysis of the incontinence visual analog and QoL scale scores showed that the majority of patients reported disappearance of incontinence together with significant improvement of their QoL. Conclusions The one-year results of this study suggest that TVT-O is associated with a low incidence of peri- and post-operative complications and high objective and subjective SUI cure rates. [less ▲]

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See detailTransobturator tape for sling procedures
Waltregny, David ULg; Reul, Olivier ULg; Bonnet, Pierre ULg et al

in AUANews (2005), 10

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See detailNew surgical technique for treatment of stress urinary incontinence TVT-Obturator: New developments and results
de Leval, Jean ULg; Waltregny, David ULg

in Surgical Technology International (2005), 14

A new surgical technique, the inside-out transobturator tension-free urethral suspension (TVT-Obturator), has been developed for treatment of women suffering from stress urinary incontinence (SUI). This ... [more ▼]

A new surgical technique, the inside-out transobturator tension-free urethral suspension (TVT-Obturator), has been developed for treatment of women suffering from stress urinary incontinence (SUI). This simple procedure uses specifically designed surgical instruments to allow the accurate passage of a synthetic tape from underneath the urethra, through the obturator foramens, toward the thigh folds, with the tape being positioned without tension under the junction between mid and distal urethra. Cadaver dissection studies have demonstrated that the anatomical trajectory of the tape in tissues is strictly perineal and consistently coursed away from neighboring neurovascular structures, including the obturator, femoral, and saphenous nerves and vessels, as well as the pudendal nerve. Consequently, as opposed to retropubic sling systems, no perioperative cystoscopy is required because the TVT-O tape does not enter the pelvic region at any time during the procedure. These anatomical data, which suggest the TVT-O technique is safe, have been corroborated by initial clinical experience. Our feasibility and ongoing efficacy studies have shown that TVT-O is associated with a low incidence of perioperative and postoperative complications. In our current series of more than 500 consecutive patients treated with TVT-O, no injury to the bladder or urethra was encountered and no perineal or obturator hematoma, heavy bleeding, or neurological complication was observed. Medium-term results have suggested that TVT-O is as efficient as retropubic tension-free slings for treatment of female SUI, with +/-90% complete cure rates after one-year follow up. [less ▲]

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See detailDentin sialophosphoprotein expression correlates with progression markers in human prostate cancer
Chaplet, Michael; Waltregny, David ULg; Detry, Cédric ULg et al

in Journal of Bone and Mineral Research (2005), 20(Suppl. 2), 45

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See detailTransobturator vaginal tape inside out for the surgical treatment of female stress urinary incontinence: anatomical considerations.
Bonnet, Pierre ULg; Waltregny, David ULg; Reul, Olivier ULg et al

in Journal of Urology (The) (2005), 173(4), 1223-8

PURPOSE: We have recently described a novel surgical technique for female stress urinary incontinence, that is the transobturator vaginal tape inside out, which uses specific instruments for the passage ... [more ▼]

PURPOSE: We have recently described a novel surgical technique for female stress urinary incontinence, that is the transobturator vaginal tape inside out, which uses specific instruments for the passage of a synthetic tape from beneath the urethra toward the thigh folds. Herein we report the results of cadaver dissection performed to determine the anatomical trajectory of the tape and its relationships with neighboring neurovascular structures and organs. MATERIALS AND METHODS: Insertion of the transobturator vaginal tape inside out tape was performed by different surgeons in 12 freshly frozen female cadavers according to the standard procedure. The thigh, obturator, perineal and pelvic regions were dissected and tape trajectory was recorded. An additional cadaver was dissected without prior tape placement. RESULTS: The tape was inserted according to a certain consistent path, that is penetration from the suburethral space into a strictly perineal region limited medial and cranial by the levator ani muscle, caudal by the perineal membrane and lateral by the obturator internus muscle. This region corresponded to the most anterior recess of the ischiorectal fossa. The tape then perforated the obturator membrane and muscles, and exited through the skin after traversing adductor muscles and subcutaneous tissue. The tape was coursed away from 1) the dorsal nerve to the clitoris located more superficially below the perineal membrane, 2) the obturator nerve and vessels, and 3) the saphenous and femoral vessels. CONCLUSIONS: These findings strongly suggest that our transobturator technique is highly accurate, reproducible and safe, and it does not require perioperative cystoscopy. [less ▲]

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See detailHidden focal abnormalities of smooth muscles in human GI motility disorders revealed by novel immunomarkers
Wedel, Théo; van Eys, G.; Glénisson, Wendy et al

Conference (2005)

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