References of "Waltregny, David"
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See detailThe biology of HDAC8
Waltregny, David ULg; Castronovo, Vincenzo ULg

Book published by The Humana Press Inc. (2006)

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See detailExpression of dentin sialophosphoprotein in human prostate cancer and its correlation with tumor aggressiveness
Chaplet, Michael; Waltregny, David ULg; Detry, Cédric ULg et al

in International Journal of Cancer = Journal International du Cancer (2006), 118(4), 850-856

Recent studies have demonstrated that two SIBLING family members, bone sialoprotein (BSP) and osteopontin (OPN), are overexpressed in human prostate cancer. The expression of these proteins is associated ... [more ▼]

Recent studies have demonstrated that two SIBLING family members, bone sialoprotein (BSP) and osteopontin (OPN), are overexpressed in human prostate cancer. The expression of these proteins is associated with the acquisition of a metastatic phenotype by cancer cells and a poor prognosis for the patient. Dentin sialophosphoprotein (DSPP) shares several structural and genetic features with OPN and BSP. The presence of DSPP has been recently established in salivary glands, indicating that its expression is not restricted to mineralized tissues. However, its potential expression in human tumors has not been addressed yet. In this study, we sought to evaluate the expression of DSPP in human prostate cancer. Immunohistochemistry was performed on 69 prostate cancer specimens using LFMb-21 anti-DSPP monoclonal antibody. All of the prostate cancer lesions examined expressed detectable levels of DSPP, as compared with no or low level of expression in adjacent normal glands (p < 0.0001). High grade prostatic intraepithelial neoplasia (HGPIN) glands generally displayed DSPP expression levels that were similar to those found in neighboring cancer glands. DSPP expression was significantly associated with the pathological stage (p = 0.0087) and the Gleason score (p = 0.0176) of the tumors. Western Blot was performed on 5 representative prostate tumor extracts and 3 prostatic tumor cell lines (PC3, LNCaP and DU145). All tumor extracts and cell lines analyzed have been found to express DSPP. In addition, in situ hybridization was used to assess the presence of DSPP mRNA. DSPP was detected at the RNA level in both HGPIN and tumoral glands. This study shows for the first time that DSPP is ectopically expressed in human prostate cancer. The expression of this SIBLING protein strongly correlates with conventional histopathological prognostic indicators of prostate cancer progression. (c) 2005 Wiley-Liss, Inc. [less ▲]

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See detailHistone deacetylase HDAC7 as a key regulator of the angiogenic process
Mottet, Denis; Bellahcene, Akeila ULg; Waltregny, David ULg et al

Poster (2006, January 20)

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See detailUterine tumors resembling ovarian sex-cord tumors: A study of 14 cases showing a diverse phenotypic profile
de Leval, Laurence ULg; Waltregny, David ULg; Dupuis, Léon-Marie et al

in Laboratory Investigation : Journal of Technical Methods & Pathology (2006, January), 86(Suppl. 1), 176

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See detailUterine tumors resembling ovarian sex-cord tumors: A study of 14 cases showing a diverse phenotypic profile
de Leval, Laurence ULg; Waltregny, David ULg; Dupuis, Léon-Marie et al

in Modern Pathology (2006, January), 19(Suppl. 1), 176

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See detailAndrogens regulation of the ERBB1 and ERBB2 oncogene expression in human prostate cancer cells
Pignon, Jean-Christophe; Delacroix, Laurence; Nolens, Grégory et al

Poster (2006)

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See detailHistone deacetylase HDAC8 associates with smooth muscle alpha–actin and is essential for smooth muscle cell contractility
Glénisson, Wendy; Waltregny, David ULg; Tran, Syv Li et al

Conference (2006)

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See detailStudy of the role of histone deacetylases in myofibroblastic differenciation
Glénisson, Wendy; Waltregny, David ULg; Castronovo, Vincenzo ULg

Scientific conference (2006)

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See detailEvaluation of original dual thromboxane A2 modulators as anti-angiogenic agents
Dassesse, Thibaut; de Leval, Xavier; Dogné, Jean-Michel et al

Scientific conference (2006)

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See detailIdentification of accessible proteins expressed in human breast cancer
Castronovo, Vincenzo ULg; Kischel, Philippe; Guillonneau, François et al

Scientific conference (2006)

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See detailRegulation by androgens of EGF receptor family members in prostate cancer cells
Pignon, Jean-Christophe; Delacroix, Laurence ULg; Waltregny, David ULg et al

in Proceedings of the American Association for Cancer Research (2006)

After an initial positive response to anti-androgen treatment, prostate cancer (PCa) cells usually become hormone-refractory in spite of their persistent expression of the androgen receptor (AR ... [more ▼]

After an initial positive response to anti-androgen treatment, prostate cancer (PCa) cells usually become hormone-refractory in spite of their persistent expression of the androgen receptor (AR). Overexpression of tyrosine kinase receptors in androgen-deprived PCa cells, such as those of the EGF receptor (EGFR) family, may be responsible for AR activation and growth of androgen-deprived tumours. Our goal is to understand the control of the expression of the EGFR family members by androgens in PCa. Hormone response was compared in hormone-sensitive LNCaP and hormone-insensitive DU145 PCa cell lines. These cells do not express ErbB4. EGFR, erbB2 and erbB3 protein half-life is much longer in DU145 than in LNCaP cells grown in complete medium. Dihydrotestosterone (DHT) modulates EGFR and erbB2 transcript and protein levels only in LNCaP cells. ErbB3 is not an androgen-responsive gene. EGFR mRNA and protein levels are increased while erbB2 mRNA and protein levels are decreased after DHT treatment of cells cultured in steroid-deprived medium. ErbB2 mRNA and protein levels are increased in LNCaP cells following DHT withdrawal. In order to understand the mechanisms by which androgens control the expression of EGFR and ERBB2 genes, half lifes of the corresponding mRNAs and proteins were compared in cells grown in presence or absence of DHT. The effect of DHT on EGFR gene expression is complex. Indeed, DHT stabilizes the protein. Moreover, a superinduction of EGFR mRNA was observed in cells treated with cycloheximide (CHX) before addition of the hormone, suggesting an effect on transcript stability. In contrast, erbB2 mRNA and protein stability was not affected by DHT. CHX treatment for 2h before addition of DHT suppresses the androgen-induced down-regulation of erbB2 mRNA levels. In summary, androgen-mediated regulation of EGFR and ERBB2 genes expression is complex. DHT influences EGFR gene transcription, mRNA and protein stability. DHT does not affect erbB2 mRNA and protein stability but acts indirectly on transcription. Current experiments are undertaken to verify these observations by Chromatin-IP experiments on both genes promoters. [less ▲]

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See detailHistone deacetylase 7 is involved in the control of angiogenesis by regulating platelet-derived growth factor-β
Mottet, Denis; Bellahcene, Akeila ULg; Deroanne, Christophe et al

Conference (2006)

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See detailRole of tumor-associated lipogenesis in the formation and composition of membrane microdomains
Timmermans, L.; Kischel, Philippe; Beckers, Albert ULg et al

Conference (2006)

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See detailRole of histone deacetylases in myofibroblastic differenciation
Glénisson, Wendy; Waltregny, David ULg; Castronovo, Vincenzo ULg

Conference (2006)

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See detailAndrogens regulation of the ERBB1 and ERBB2 oncogene expression in human prostate cancer cells
Pignon, Jean-Christophe ULg; Delacroix, Laurence; Waltregny, David ULg et al

in Acta Clinica Belgica (2006), 61(2, MAR-APR), 95-95

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See detailExamination of the non-palpable cryptorchid testis
Waltregny, David ULg

Conference (2005, June)

La cryptorchidie affecre environ 1 garçon sur 150 et dans près de 20% des cas, la gonade n'est pas palpée. Lorsqu'il est présent, le testicule non palpable est situé entre l'orifice externe du canal ... [more ▼]

La cryptorchidie affecre environ 1 garçon sur 150 et dans près de 20% des cas, la gonade n'est pas palpée. Lorsqu'il est présent, le testicule non palpable est situé entre l'orifice externe du canal inguinal et le pôle inférieur du rein. Souvent cependant, lors de l'exploration chirurgicale, on constate l'existence de reliquats tissulaires à l'extrémité de vaisseaux spermatiques borgnes sans que l'on puisse reconnaître à proprement parler un testicule: on parle alors de testicule absent. Dans d'autres cas, on ne trouve pas de trace de vaisseau spermatique, de structure épidydimo-déférentielle ou encore de tissu testiculaire; il s'agit d'agénésie gonadique. Le testicule non palpé présente un risque accru d'infertilité, de transformation maliine, de hernies et malformations épididymo-déférentielles associées. Le traitement chirurgical est souvent plus complexe et les échecs plus fréquents. De nombreuses techniques d'imagerie ont été proposées comme modalités diagnostiques pour localiser le/les testicule(s) non palpé(s) (pneumopéritonéographie, angiographie, ultrasonographie, tomodensitométrie computérisée, RMN) mais aucune d'elles, à ce jour, n'est uniformément acceptée en raison du manque de fiabilité à détecter le testicule recherché et/ou des dangers potentiels résultant de leur emploi. Depuis la description par Cortesi en 1976 de l'utilisation de la cælioscopie pour la localisation du testicule impalpable, un intérêt croissant a été porté par les urologues et les chirurgiens pédiatriques à cette technique qui aujourd'hui joue un rôle central dans l'exploration du testicule cryptorchide non palpé. Les auteurs tentent d'établir une méthodologie algorythmique d'investigation des testicules cryptorchides non palpés à la lumière des données anatomiques, pathologiques et chirurgicales récentes de la littérature. [less ▲]

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See detailExpression and nuclear location of the transcriptional repressor kaiso is regulated by the tumor microenvironment
Soubry, Adelheid; van Hengel, Jolanda; Parthoens, Eef et al

in Cancer Research (2005), 65(6), 2224-2233

Kaiso is a BTB/POZ zinc finger protein originally described as an interaction partner of p120ctn. In cultured cell lines, Kaiso is found almost exclusively in the nucleus, where it generally acts as a ... [more ▼]

Kaiso is a BTB/POZ zinc finger protein originally described as an interaction partner of p120ctn. In cultured cell lines, Kaiso is found almost exclusively in the nucleus, where it generally acts as a transcriptional repressor. Here, we describe the first in situ immunolocalization studies of Kaiso expression in normal and cancerous tissues. Surprisingly, we found striking differences between its behavior in monolayers of different cell lines, three-dimensional cell culture systems, and in vivo. Although nuclear localization was sometimes observed in tissues, Kaiso was more often found in the cytoplasm, and in some cell types it was absent. In general, Kaiso and p120ctn did not colocalize in the nucleus. To examine this phenomenon more carefully, tumor cells exhibiting strong nuclear Kaiso staining in vitro were injected into nude mice and grown as xenografts. The latter showed a progressive translocation of Kaiso towards the cytoplasm over time, and even complete loss of expression, especially in the center of the tumor nodules. When xenografted tumors were returned to cell culture, Kaiso was re-expressed and was once again found in the nucleus. Translocation of Kaiso to the cytoplasm and down-regulation of its levels were also observed under particular experimental conditions in vitro, such as formation of spheroids and acini. These data strongly imply an unexpected influence of the microenvironment on Kaiso expression and localization. As transcriptional repression is a nuclear event, this phenomenon is likely a crucial factor in the regulation of Kaiso function. [less ▲]

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