References of "Waltregny, David"
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See detailLipomatose pelvienne associee a une cystite glandulaire: rapport de deux cas
Leruth, Julie ULg; Coppens, Luc ULg; Andrianne, Robert ULg et al

in Progrès en Urologie (2005), 15(1), 81-4

The authors report two cases of pelvic lipomatosis associated with glandular cystitis. This is a rare disease with variable and nonspecific clinical features. Complementary investigations useful for the ... [more ▼]

The authors report two cases of pelvic lipomatosis associated with glandular cystitis. This is a rare disease with variable and nonspecific clinical features. Complementary investigations useful for the diagnosis of pelvic lipomatosis are CT and especially MRI, intravenous urography and biopsies. The clinical features, radiological findings and therapeutic approach are discussed in the light of a review of the literature. [less ▲]

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See detailInside-out Transobturator Vaginal Tape (TVT-O): one-year results of a prospective study
Waltregny, David ULg; Reul, Olivier ULg; Keppenne, Véronique ULg et al

in European Urology Supplements (2005), 4(3), 1653

Title Inside-out transobturator vaginal tape (TVT-O): One-year results of a prospective study Introduction and Objectives The aim of this study was to prospectively assess the efficacy of a new surgical ... [more ▼]

Title Inside-out transobturator vaginal tape (TVT-O): One-year results of a prospective study Introduction and Objectives The aim of this study was to prospectively assess the efficacy of a new surgical technique, the inside-out transobturator vaginal tape (TVT-O), for the treatment of female stress urinary incontinence (SUI). Study design, materials and methods From 03/2003 through 10/2003, a TVT-O tape was inserted in 83 consecutive patients with clinical evidence of SUI. Preoperative evaluation included complete history, physical examination, urodynamics, urine analysis, and cystoscopy. None of the patients presented the following exclusion criteria: post-void residual (PVR) >100 cc, detrusor overactivity or acontractility, pregnancy, neurological pathology, active urinary or vaginal infection, age >85 years, negative stress test, and maximum cystometric capacity <300 mL. Post-operative evaluation was carried out using symptom scoring and quality of life (QoL) questionnaires, visual analog scales, physical examination, uroflowmetry, and PVR measurement. Cure was defined as no leakage based on both symptom scale scoring and physical examination. Improvement was defined as ≥50% decrease in symptoms based on the questionnaire’s results. Results Mean age of the patients was 61 years. The TVT-O procedure was associated with pelvic organ prolapse cure in 15 patients (18%). Follow-up time was ≥12 months in all women (mean = 13.6); 3 patients were lost to follow-up. No significant blood loss (≥100 cc), vaginal wall, urethral, or bladder perforation was encountered. No hematoma, vaginal or urethral erosion, or neurological complication was observed. No patient complained of persistent pain. At the latest follow-up visit, max flow rate was ≥10 mL/sec and PVR was <100 cc in 90% and 94% patients, respectively. Two patients underwent an immediate tape release procedure while the tape was sectioned in 2 other patients for retention and/or urgency associated with obstruction. Sixty eight patients (85%) were cured of their SUI while 9 patients (11%) were improved. Urgency questionnaire’s results showed that 5 (5/46) patients developed de novo urgency. Twenty and 14 out of the 34 patients with preoperative urge symptoms reported either disappearance or no change of urgency, respectively. Obstruction symptoms appeared or worsened in 3 patients and were unchanged or decreased in all other patients. Analysis of the incontinence visual analog and QoL scale scores showed that the majority of patients reported disappearance of incontinence together with significant improvement of their QoL. Conclusions The one-year results of this study suggest that TVT-O is associated with a low incidence of peri- and post-operative complications and high objective and subjective SUI cure rates. [less ▲]

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See detailTransobturator tape for sling procedures
Waltregny, David ULg; Reul, Olivier ULg; Bonnet, Pierre ULg et al

in AUANews (2005), 10

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See detailNew surgical technique for treatment of stress urinary incontinence TVT-Obturator: New developments and results
de Leval, Jean ULg; Waltregny, David ULg

in Surgical Technology International (2005), 14

A new surgical technique, the inside-out transobturator tension-free urethral suspension (TVT-Obturator), has been developed for treatment of women suffering from stress urinary incontinence (SUI). This ... [more ▼]

A new surgical technique, the inside-out transobturator tension-free urethral suspension (TVT-Obturator), has been developed for treatment of women suffering from stress urinary incontinence (SUI). This simple procedure uses specifically designed surgical instruments to allow the accurate passage of a synthetic tape from underneath the urethra, through the obturator foramens, toward the thigh folds, with the tape being positioned without tension under the junction between mid and distal urethra. Cadaver dissection studies have demonstrated that the anatomical trajectory of the tape in tissues is strictly perineal and consistently coursed away from neighboring neurovascular structures, including the obturator, femoral, and saphenous nerves and vessels, as well as the pudendal nerve. Consequently, as opposed to retropubic sling systems, no perioperative cystoscopy is required because the TVT-O tape does not enter the pelvic region at any time during the procedure. These anatomical data, which suggest the TVT-O technique is safe, have been corroborated by initial clinical experience. Our feasibility and ongoing efficacy studies have shown that TVT-O is associated with a low incidence of perioperative and postoperative complications. In our current series of more than 500 consecutive patients treated with TVT-O, no injury to the bladder or urethra was encountered and no perineal or obturator hematoma, heavy bleeding, or neurological complication was observed. Medium-term results have suggested that TVT-O is as efficient as retropubic tension-free slings for treatment of female SUI, with +/-90% complete cure rates after one-year follow up. [less ▲]

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See detailDentin sialophosphoprotein expression correlates with progression markers in human prostate cancer
Chaplet, Michael; Waltregny, David ULg; Detry, Cédric ULg et al

in Journal of Bone and Mineral Research (2005), 20(Suppl. 2), 45

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See detailTransobturator vaginal tape inside out for the surgical treatment of female stress urinary incontinence: anatomical considerations.
Bonnet, Pierre ULg; Waltregny, David ULg; Reul, Olivier ULg et al

in Journal of Urology (The) (2005), 173(4), 1223-8

PURPOSE: We have recently described a novel surgical technique for female stress urinary incontinence, that is the transobturator vaginal tape inside out, which uses specific instruments for the passage ... [more ▼]

PURPOSE: We have recently described a novel surgical technique for female stress urinary incontinence, that is the transobturator vaginal tape inside out, which uses specific instruments for the passage of a synthetic tape from beneath the urethra toward the thigh folds. Herein we report the results of cadaver dissection performed to determine the anatomical trajectory of the tape and its relationships with neighboring neurovascular structures and organs. MATERIALS AND METHODS: Insertion of the transobturator vaginal tape inside out tape was performed by different surgeons in 12 freshly frozen female cadavers according to the standard procedure. The thigh, obturator, perineal and pelvic regions were dissected and tape trajectory was recorded. An additional cadaver was dissected without prior tape placement. RESULTS: The tape was inserted according to a certain consistent path, that is penetration from the suburethral space into a strictly perineal region limited medial and cranial by the levator ani muscle, caudal by the perineal membrane and lateral by the obturator internus muscle. This region corresponded to the most anterior recess of the ischiorectal fossa. The tape then perforated the obturator membrane and muscles, and exited through the skin after traversing adductor muscles and subcutaneous tissue. The tape was coursed away from 1) the dorsal nerve to the clitoris located more superficially below the perineal membrane, 2) the obturator nerve and vessels, and 3) the saphenous and femoral vessels. CONCLUSIONS: These findings strongly suggest that our transobturator technique is highly accurate, reproducible and safe, and it does not require perioperative cystoscopy. [less ▲]

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See detailHidden focal abnormalities of smooth muscles in human GI motility disorders revealed by novel immunomarkers
Wedel, Théo; van Eys, G.; Glénisson, Wendy et al

Conference (2005)

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See detailHistone deacetylase HDAC8 associates with smooth muscle alpha-actin and is essential for smooth muscle cell contractility
Waltregny, David ULg; Glenisson, Wendy; Tran, Syv Li et al

in FASEB Journal (2005), 19(8), 966-968

Although originally characterized as nuclear enzymes controlling the stability of nucleosomes, histone deacetylases (HDACs) may also exert their activity within the cytosol. Recently, we have demonstrated ... [more ▼]

Although originally characterized as nuclear enzymes controlling the stability of nucleosomes, histone deacetylases (HDACs) may also exert their activity within the cytosol. Recently, we have demonstrated that HDAC8, a class I HDAC, is a novel, prominently cytosolic marker of smooth muscle differentiation. As HDAC8 displays a striking stress fiber-like pattern of distribution and is coexpressed in vivo with smooth muscle alpha-actin (alpha-SMA) and smooth muscle myosin heavy chain, we have explored the possible participation of this HDAC in smooth muscle cytoskeleton regulation. Cell fractionation assays performed with primary human smooth muscle cells (HSMCs) showed that HDAC8, in contrast to HDAC1 and HDAC3, was enriched in cytoskeleton-bound protein fractions and insoluble cell pellets, suggesting an association of HDAC8 with the cystoskeleton. Coimmunoprecipitation experiments using HSMCs, NIH-3T3 cells, and human prostate tissue lysates further demonstrated that HDAC8 associates with alpha-SMA but not with beta-actin. HDAC8 silencing through RNA interference strongly reduced the capacity of HSMCs to contract collagen lattices. Mock transfections had no effect on HSMC contractily, and transfections with small interfering RNAs (siRNAs) specific for HDAC6, a cytosolic HDAC that functions as an alpha-tubulin deacetylase, resulted in a weak contraction inhibition. Although mock- and HDAC6 siRNA-transfected HSMCs showed no noticeable morphological changes, HDAC8 siRNA-transfected HSMCs displayed a size reduction with diminished cell spreading after replating. Altogether, our findings indicate that HDAC8 associates with the smooth muscle actin cytoskeleton and may regulate the contractile capacity of smooth muscle cells. [less ▲]

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See detailHistone deacetylase HDAC8 associates with smooth muscle alpha–actin and is essential for smooth muscle cell contractility
Glénisson, Wendy; Waltregny, David ULg; Tran, Syv Li et al

Conference (2005)

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See detailAbnormalities of smooth muscles in human GI motility disorders revealed by novel immunomarkers
Wedel, Theo; van Eys, G.; Glénisson, Wendy et al

Poster (2005)

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See detailRéponse à : Chirurgie de l'incontinence urinaire a l'effort feminine par voie transobturatrice: dehors dedans ou dedans dehors? etude anatomique comparative.
de Leval, Jean ULg; Bonnet, Pierre ULg; Waltregny, David ULg et al

in Progrès en Urologie : Journal de l'Association Française d'Urologie et de la Société Française d'Urologie (2005), 15(6), 1161-2

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See detailExpression of dentin sialophosphoprotein in human prostate cancer and its correlation with tumor aggressiveness
Waltregny, David ULg; Chaplet, Michael; Detry, Cédric et al

Conference (2005)

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See detailDentin sialophosphoprotein expression correlates with progression markers in human prostate cancer
Chaplet, Michael; Waltregny, David ULg; Detry, Cédric et al

Conference (2005)

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See detailLignées de carcinome prostatique et apoptose: état de la question
Califice, Stéphane ULg; Waltregny, David ULg; Castronovo, Vincenzo ULg et al

in Revue Médicale de Liège (2004), 59(12), 704-10

Prostate cancer is a major pathology in industrialized countries. Tumor growth usually results from increased cell proliferation, conjugated with an inhibition of programmed cell death (apoptosis). In ... [more ▼]

Prostate cancer is a major pathology in industrialized countries. Tumor growth usually results from increased cell proliferation, conjugated with an inhibition of programmed cell death (apoptosis). In this paper, after a short description of the apoptotic mechanisms and their methods of investigation, we review the present knowledge of the implication of different molecular actors in the regulation of apoptosis in prostate cancer cells. This review notably summarizes the present knowledge of the (de)regulation of the effects of androgens, p53, Bcl-2, Bcl-xL, Bax, Akt, PTEN, Par-4, clusterine, caspases and NF-kappaB in prostate adenocarcinoma cell lines and provides an appraisal of their therapeutic potential. A better knowledge of the apoptotic pathways in these cells could indeed allow the development of new selective and effective anti-cancer strategies. [less ▲]

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See detailExpression of histone deacetylase 8, a class I histone deacetylase, is restricted to cells showing smooth muscle differentiation in normal human tissues
Waltregny, David ULg; de Leval, Laurence ULg; Glenisson, Wendy et al

in American Journal of Pathology (2004), 165(2), 553-564

Histone deacetylases (HDACs) were originally identified as nuclear enzymes involved in gene transcription regulation. Until recently, it was thought that their activity was restricted within the nucleus ... [more ▼]

Histone deacetylases (HDACs) were originally identified as nuclear enzymes involved in gene transcription regulation. Until recently, it was thought that their activity was restricted within the nucleus, with histones as unique substrates. The demonstration that specific HDACs deacetylate nonhistone proteins, such as p53 and alpha-tubulin, broadened the field of activity of these enzymes. HDAC8, a class I HDAC, is considered to be ubiquitously expressed, as suggested by results of Northern blots performed on tissue RNA extracts, and transfection experiments using various cell lines have indicated that this enzyme may display a prominent nuclear localization. Using immunohistochemistry, we unexpectedly found that, in normal human tissues, HDAC8 is exclusively expressed by cells showing smooth muscle differentiation, including visceral and vascular smooth muscle cells, myoepithelial cells, and myofibroblasts, and is mainly detected in their cytosol. These findings were confirmed in vitro by nucleo-cytoplasmic fractionation and immunoblot experiments performed on human primary smooth muscle cells, and by the cytosolic detection of epitope-tagged HDAC8 overexpressed in fibroblasts. Immunocytochemistry strongly suggested a cytoskeleton-like distribution of the enzyme. Further double-immunofluorescence staining experiments coupled with confocal microscopy analysis showed that epitope-tagged HDAC8 overexpressed in murine fibroblasts formed cytoplasmic stress fiber-like structures that co-localized with the smooth muscle cytoskeleton protein smooth muscle alpha-actin. Our works represent the first demonstration of the restricted expression of a class I HDAC to a specific cell type and indicate that HDAC8, besides being a novel marker of smooth muscle differentiation, may play a role in the biology of these contractile cells. [less ▲]

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See detailNovel surgical technique for the treatment of female urinary incontinence: Transobturator vaginal tape inside-out
de Leval, Jean ULg; Bonnet, Pierre ULg; Reul, Olivier ULg et al

in European Urology Supplements (2004, February), 3(2), 226

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