Molecular cloning and functional expression of a new aphid isoprenyl diphosphate synthase

Poster (2006, December 18)

Etude moléculaire de la structure et de la fonction du produit de l'oncogène Tre2, homologue à deux protéines Ypt/Rab GAPs levuriennes, et impliqué dans différentes tumeurs humaines incluant le sarcome d'Ewing

Poster (2006)

Quantification of Bifidobacterium spp. and Lactobacillus spp. in rat fecal samples by real-time PCR.

in Microbiological research (2006), (5),

Identification of sequences responsible of lost of activity of the Tre2 oncogene product by functional complementation of the yeast msb3msb4 mutant

Poster (2005)

Expression and purification of the human Tre2 product, a putative Ypt/Rab GTPase activating protein.

in Archives Internationales de Physiologie et de Biochimie (2005), 191

Identification of sequences responsible for lost of activity of the Tre2 oncogene product by functional complementation of the yeast msb3msb4 mutant

in Yeast (Chichester, England) (2005), 22(S1), 155

Estimation of bioreactor efficiency through structured hydrodynamic modeling case study of a Pichia pastoris fed-batch process.

in Applied Biochemistry and Biotechnology (2005), 121-124

In this article, two theories are unified to investigate the effect of hydrodynamics on a specific bioprocess: the network-of-zones (NOZ) hydrodynamic structured modeling approach (developed by several ... [more ▼]

In this article, two theories are unified to investigate the effect of hydrodynamics on a specific bioprocess: the network-of-zones (NOZ) hydrodynamic structured modeling approach (developed by several researchers but applied to only a few bioprocesses) and the effectiveness factor eta approach. Two process scales were investigated (20 and 500 L), and for each, hydrodynamics were quantified using an NOZ validated by homogeneity time measurements. Several impeller combinations inducing quite different hydrodynamics were tested at the 20-L scale. After this step, effectiveness factors were determined for each fermentation run. To achieve this, a perfectly mixed microbial kinetic model was evaluated by using simple Monod kinetics with a fed-batch mass balance. This methodology permitted determination of the effectiveness factor with more accuracy because of the relation with the perfect case deduced from the Monod kinetics. It appeared that for the small scale, eta decreased until reaching a value of approx 0.7 (30% from the ideal case) for the three impeller systems investigated. However, stirring systems that include hydrofoils seemed to maintain higher effectiveness factors during the course of the fermentation. This effect can be attributed to oxygen transfer performance or to homogenization efficiency exhibited by the hydrofoils. To distinguish the oxygen transfer from the homogenization component of the effectiveness factor, these phenomena were analyzed separately. After determining the evolution of etaO2 linked to oxygen transfer for each of the fermentation runs, the NOZ model was employed to quantify substrate gradient appearance. After this step, another effectiveness factor, etamix, related to mixing was defined. Consequently, it is possible to distinguish the relative importance of the mixing effect and oxygen transfer on a given bioprocess. The results have highlighted an important scale effect on the bioprocess that can be analyzed using the NOZ model. [less ▲]

At Least Two Regions Of The Oncoprotein Tre2 Are Involved In Its Lack Of Gap Activity

in Biochemical and Biophysical Research Communications (2005), 335(3),

Implication of two genes coding for exo-ß-1,3-glucanase in the biocontrol activity of Pichia anomala (strain K) against on post-harvest apples

Conference (2005)

Development of a gene targeting technique based on URA3-blaster on P. anomalata (strain K)

Poster (2004)