References of "Van Oosterwyck, Hans"
     in
Bookmark and Share    
Full Text
Peer Reviewed
See detailCoupling Cell Mechanics and Intracellular Signalling: Mechanotransduction through ERK
Kerkhofs, Johan ULg; Geris, Liesbet ULg; Bosmans, Bart et al

in Middleton, John (Ed.) The Proceedings of the 10th International Symposium on Computer Methods in Biomechanics and Biomedical Engineering. Hotel Berlin, Berlin, Germany, April 7th – 11th, 2012 pages:0-0 (2012, April 12)

Detailed reference viewed: 11 (1 ULg)
Peer Reviewed
See detailTip cells at the top: multiscale modeling of angiogenesis during fracture healing
Carlier, Aurélie ULg; Geris, Liesbet ULg; Van Oosterwyck, Hans

in Computer Methods in Biomechanics and Biomedical Engineering (CMBBE) - Proceedings (2012, April)

Detailed reference viewed: 37 (11 ULg)
Full Text
Peer Reviewed
See detailMOSAIC: a multiscale model of osteogenesis and sprouting angiogenesis with lateral inhibition of endothelial cells.
Carlier, Aurélie ULg; Geris, Liesbet ULg; Bentley, Katie et al

in PLoS Computational Biology (2012), 8(10), 1002724

The healing of a fracture depends largely on the development of a new blood vessel network (angiogenesis) in the callus. During angiogenesis tip cells lead the developing sprout in response to ... [more ▼]

The healing of a fracture depends largely on the development of a new blood vessel network (angiogenesis) in the callus. During angiogenesis tip cells lead the developing sprout in response to extracellular signals, amongst which vascular endothelial growth factor (VEGF) is critical. In order to ensure a correct development of the vasculature, the balance between stalk and tip cell phenotypes must be tightly controlled, which is primarily achieved by the Dll4-Notch1 signaling pathway. This study presents a novel multiscale model of osteogenesis and sprouting angiogenesis, incorporating lateral inhibition of endothelial cells (further denoted MOSAIC model) through Dll4-Notch1 signaling, and applies it to fracture healing. The MOSAIC model correctly predicted the bone regeneration process and recapitulated many experimentally observed aspects of tip cell selection: the salt and pepper pattern seen for cell fates, an increased tip cell density due to the loss of Dll4 and an excessive number of tip cells in high VEGF environments. When VEGF concentration was even further increased, the MOSAIC model predicted the absence of a vascular network and fracture healing, thereby leading to a non-union, which is a direct consequence of the mutual inhibition of neighboring cells through Dll4-Notch1 signaling. This result was not retrieved for a more phenomenological model that only considers extracellular signals for tip cell migration, which illustrates the importance of implementing the actual signaling pathway rather than phenomenological rules. Finally, the MOSAIC model demonstrated the importance of a proper criterion for tip cell selection and the need for experimental data to further explore this. In conclusion, this study demonstrates that the MOSAIC model creates enhanced capabilities for investigating the influence of molecular mechanisms on angiogenesis and its relation to bone formation in a more mechanistic way and across different time and spatial scales. [less ▲]

Detailed reference viewed: 19 (3 ULg)
Peer Reviewed
See detailµCT-Based, Anatomical Finite Element Model of a Unilateral Fixator for Bone Reconstruction in Rabbit Tibia
Karunratanakul, Kavin; Kerckhofs, Greet ULg; Lammens, Johan et al

Conference (2012)

Detailed reference viewed: 8 (1 ULg)
Peer Reviewed
See detailMultiscale modeling of sprouting angiogenesis
Carlier, Aurélie ULg; Geris, Liesbet ULg; Van Oosterwyck, Hans

Poster (2011, December 02)

Detailed reference viewed: 24 (9 ULg)
Peer Reviewed
See detailCalcium phosphate scaffolds customizations for bone tissue engineering applications
Carlier, Aurélie ULg; Chai, Yoke Chin; Theys, Tina et al

Poster (2011, November 18)

Detailed reference viewed: 18 (4 ULg)
Peer Reviewed
See detailA Boolean network approach to developmental engineering
Kerkhofs, Johan ULg; Roberst, Scott J; Luyten, Frank P et al

Conference (2011, June 13)

Detailed reference viewed: 11 (0 ULg)
Peer Reviewed
See detailAn integrative model based approach to optimize calcium phosphate scaffold-stem cell combinations
Carlier, Aurélie ULg; Chai, Yoke Chin; Theys, Tina et al

Poster (2011, June 07)

Detailed reference viewed: 10 (1 ULg)
Peer Reviewed
See detailOptimization of calcium phosphate scaffold-cell combinations using an integrative model based approach
Carlier, Aurélie ULg; Chai, Yoke Chin; Theys, Tina et al

in International Conference on Tissue Engineering (ECCOMAS) - proceedings (2011, June 03)

Detailed reference viewed: 13 (5 ULg)
Peer Reviewed
See detailFluorescent microparticle incorporation in chondrogenic cell aggregates to monitor local oxygen tension
Lambrechts, Dennis; Kerckhofs, Greet ULg; Roberts, Scott et al

in Histology and Histopathology (2011)

Peer Reviewed
See detailCAD versus micro-CT based models of Ti6Al4V scaffolds for bone tissue engineering: a solid and fluid mechanical analysis
Truscello, Silvia; Kerckhofs, Greet ULg; Moesen, Maarten et al

in Proceedings of II International Conference on Tissue Engineering (2011)

Detailed reference viewed: 3 (1 ULg)
Peer Reviewed
See detailA computational solid and fluid mechanical analysis of CAD-versus micro-CT-based models of regular Ti6Al4V scaffolds for bone tissue engineering
Truscello, Silvia; Kerckhofs, Greet ULg; Moesen, Maarten et al

in Histology and Histopathology (2011)

Detailed reference viewed: 5 (1 ULg)
Peer Reviewed
See detailA Boolean network model of the growth plate
Kerkhofs, Johan ULg; Roberts, Scott J; Luyten, Frank P et al

Poster (2010, November 26)

Detailed reference viewed: 8 (0 ULg)
Peer Reviewed
See detailComputational modelling of calcium mediated bone regeneration
Carlier, Aurélie ULg; Chai, Yoke Chin; Theys, Tina et al

Poster (2010, November 26)

Detailed reference viewed: 11 (2 ULg)
Peer Reviewed
See detailA mathematical model of calcium ion influence on the activity of osteogenic cells
Carlier, Aurélie ULg; Chai, Yoke Chin; Theys, Tina et al

Poster (2010, November 19)

Detailed reference viewed: 18 (3 ULg)
Peer Reviewed
See detailA Boolean network model of the growth plate
Kerkhofs, Johan ULg; Roberts, Scott J; Luyten, Frank P et al

Poster (2010, October 10)

Detailed reference viewed: 7 (0 ULg)
Peer Reviewed
See detailBMP signalling in growth plate chondrocytes: a Boolean modelling approach
Kerkhofs, Johan ULg; Roberts, Scott J; Van Oosterwyck, Hans et al

Poster (2010, September 15)

Detailed reference viewed: 8 (0 ULg)
Full Text
Peer Reviewed
See detailOccurrence and Treatment of Bone Atrophic Non-Unions Investigated by an Integrative Approach
Geris, Liesbet ULg; Reed, Anita A.C.; Vander Sloten, Jos et al

in PLoS Computational Biology (2010), 6(9), 1000915

Recently developed atrophic non-union models are a good representation of the clinical situation in which many nonunions develop. Based on previous experimental studies with these atrophic non-union ... [more ▼]

Recently developed atrophic non-union models are a good representation of the clinical situation in which many nonunions develop. Based on previous experimental studies with these atrophic non-union models, it was hypothesized that in order to obtain successful fracture healing, blood vessels, growth factors, and (proliferative) precursor cells all need to be present in the callus at the same time. This study uses a combined in vivo-in silico approach to investigate these different aspects (vasculature, growth factors, cell proliferation). The mathematical model, initially developed for the study of normal fracture healing, is able to capture essential aspects of the in vivo atrophic non-union model despite a number of deviations that are mainly due to simplifications in the in silico model. The mathematical model is subsequently used to test possible treatment strategies for atrophic non-unions (i.e. cell transplant at post-osteotomy, week 3). Preliminary in vivo experiments corroborate the numerical predictions. Finally, the mathematical model is applied to explain experimental observations and identify potentially crucial steps in the treatments and can thereby be used to optimize experimental and clinical studies in this area. This study demonstrates the potential of the combined in silico-in vivo approach and its clinical implications for the early treatment of patients with problematic fractures. [less ▲]

Detailed reference viewed: 72 (21 ULg)
Peer Reviewed
See detailExperimentally-informed Mathematical Modelling of Oxygen Tension, Cell Viability and Proliferation in Fibrin Hydrogels
Demol, Jan; Lambrechts, Dennis; Geris, Liesbet ULg et al

in Proceedings of the TERMIS-EU meeting (2010, June)

Detailed reference viewed: 10 (1 ULg)
Peer Reviewed
See detailCross-talk Modeling of Wnt, BMP and ERK Pathways during Osteochondrogenic Differentiation
Geris, Liesbet ULg; Vandeput, M.; Roberts, S. et al

in Proceedings of the TERMIS-EU meeting (2010, June)

Detailed reference viewed: 17 (2 ULg)