References of "Touati, Kamal"
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See detailClonage par greffe de blastomère: naissance d'un premier veau
Ectors, Fabien ULg; Delval, Alain; Touati, Kamal ULg et al

in Annales de Médecine Vétérinaire (1993), 137(8), 573-574

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See detailClonage par transfert de noyau dans l'espèce bovine: premiers résultats
Ectors, Fabien ULg; Delval, Alain; Touati, Kamal ULg et al

in Annales de Médecine Vétérinaire (1993), 137(6), 427-431

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See detailComparison between culture of bovine embryos in vitro versus development in rabbit oviducts and in vivo
Ectors, Fabien ULg; Thonon, Fabienne; Delval, Alain et al

in Livestock Production Science (1993), 36

The aim of this work was to evaluate the efficiency of different embryo culture methods for in vitro embryo production: development in co-culture with bovine oviductal epithelial cells (BOEC), in BOEC ... [more ▼]

The aim of this work was to evaluate the efficiency of different embryo culture methods for in vitro embryo production: development in co-culture with bovine oviductal epithelial cells (BOEC), in BOEC conditioned medium (CM) or in rabbit oviducts, versus in vivo produced embryos. There was no significant difference in terms of percentages of cleaved and 8-cell stages obtained between CM and BOEC. In CM, 24.8% of the cleaved embryos became blastocysts. In BOEC, 14.5% of the cleaved embryos became blastocysts. Among the 190 zygotes transferred in the rabbit oviducts, 127 have been recovered 5 days later, and 17.4% became blastocysts. There was no significant difference in term of blastocyst formation between the development in rabbit and in BOEC. However, there was a significant difference between the CM group and the two other groups. The numbers of cells in blastocysts from different sources were investigated: in vivo blastocysts contained 107 cells assumed to be 100%, in vitro blastocysts developed in rabbit oviduct 100.1 cells (93.3%), BOEC blastocysts 90.8 cells (84.6%) and CM blastocysts 72.3 cells (67.3%). This study confirmed earlier works on the oviduct effect on blastocyst quality in terms of development rate and cell number. [less ▲]

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See detailLe sexage d'embryons bovins par la technique de l'amplification en chaîne par la polymérase (acp) d'une séquence Y-spécifique bovine
Grobet, Luc ULg; Schwers, Anne; Charlier, Carole ULg et al

in Annales de Médecine Vétérinaire (1992), 136

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See detailMultiplication des embryons chez les bovins: possibilités actuelles et futures
Ectors, Francis ULg; Beckers, Jean-François ULg; Ectors, Fabien ULg et al

in Biotechnologie en Sélection animale - IRSIA (1989)

Since several years, research efforts have been focused towards improving the reproductive potential of domestic animals. The authors present a review of different technics in particular the ... [more ▼]

Since several years, research efforts have been focused towards improving the reproductive potential of domestic animals. The authors present a review of different technics in particular the superovulation and priming, embryo micromanipulation and clonage. The technic of superovulation allows an increase of the total number fo the offspring by an increase of the ovocytes total number. Different FSH injections at the end of the cycle, preceded by small FSH quantity at the beginning of the cycle, procure a great number of mature follicles available for reproduction. Micromanipulation allows a multiplicaton by sectioning the embryo in two or more segments. Isolated blastmeres can also be used but it was observed that those cells very rapidly loose their differentiation potential. Clonage allows the reproduction of an animal from only one of his own cells. The nucleus of this cell is extracted and reimplanted in an enucleated ovocyte. Meanwhile from a practical point of vue, this technic is, at present, only possible using a stem cell and not using a mature somatic cell. [less ▲]

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See detailDemonstrationof somatogenic receptors in bovine corpus luteum: conjectural effect of trophic hormone on follicular growth and development.
Beckers, Jean-François ULg; Wouters-Ballman, P.; Viviers-Donnay, I. et al

Poster (1989, September 09)

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See detailRelation inverse entre la concentration des sérums foetaux bovins en hormone chorionique somatomammotrope et leur âge.
Beckers, Jean-François ULg; Wouters-Ballman, P; Ectors, Fabien ULg et al

in Reproduction Nutrition Development (1989), 29(1), 29

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See detailVitrification of bovine blastocysts
Vanderzwalmen, Pierre ULg; Touati, Kamal ULg; Ectors, Fabien ULg et al

in Theriogenology (1989), 31(1), 270

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See detailLow doses of FSH stimulate follicular growth and maturation in anestrous heifers.
Beckers, Jean-François ULg; Ballman-Wouters, P; Viviers-Donnay, I et al

in Theriogenology (1989)

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See detailVariable composition of bovine fetal calf serum: a prospective study for in vitro oocytes and embryos culture.
Beckers, Jean-François ULg; Ballman-Wouters, P; Ectors, Fabien ULg et al

in Proceedings of the 5th Annual Meeting AETE (1989)

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See detailMaturation et fécondation in vitro chez les bovins: naissance d'un veau
Ectors, Fabien ULg; Van Der Zwalmen, P.; Touati, Kamal ULg et al

in Annales de Médecine Vétérinaire (1989), 133

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See detailEffet d'une préstimulation ovarienne en début de cycle sur la réponse au traitement de superovulation chez la vache
Touati, Kamal ULg; Bormans, M.; Ectors, Fabien ULg et al

in Annales de Médecine Vétérinaire (1989), 133

In this study, we have induced superovulation in 67 heifers according to a known based treatment based on the administration of 32 mg (Armour Units) of FSH/LH (ratio LH/FSH=20%). The animals were divided ... [more ▼]

In this study, we have induced superovulation in 67 heifers according to a known based treatment based on the administration of 32 mg (Armour Units) of FSH/LH (ratio LH/FSH=20%). The animals were divided in two groups: 30 control and 37 receiving 2.5mg (Armour units) of pure FSH on the third day and the fourth day of the estrous cycle. This pretreatment ("priming") was followed by a larger number of total (11.7 versus 6.4) and transferable (7.1 versus 3.6) embryos. This experiment demonstrates the efficiency of low doses of pure FSH administered 6 or 8 dyas before a superovulation in the final selection of ovarian follicles. [less ▲]

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See detailMise au point sur la folliculogenèse
Touati, Kamal ULg; Ectors, Fabien ULg; Beckers, Jean-François ULg et al

in Annales de Médecine Vétérinaire (1989), 133

Folliculogenesis is usually divided in two stages: the first one going from the primordial follicle to the secondary or preantral, the second leading the preantral follicle to the preovulatory or Graafian ... [more ▼]

Folliculogenesis is usually divided in two stages: the first one going from the primordial follicle to the secondary or preantral, the second leading the preantral follicle to the preovulatory or Graafian follicle. During the first period, follicular development is depending on intra ovarian factors mainly of growth-factors. However, many studies involve the FSH hormone in this period, when follicles have reached the 40 cells stage. During the second period, FSH plays an essential role in follicular growth and maturation. Regulatory process for FSH secretion and liberation depends mainly on inhibin. This fact is underlined in many species by typical FSH secretion profiles. The FSH levels can explain the ovarian control leading to one or to a limited number of preovulatory follicles according to the species characteristic (dominant follicle). [less ▲]

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See detailResearch work for an optimization of an in vitro maturation and fertilization method of the bovine oocytes
Ectors, F. J.; Van Der Zwalmen, P.; Touati, Kamal ULg et al

Conference (1988, December 13)

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See detailDemonstration of somatogenic receptors in bovine corpus luteum
Beckers, Jean-François ULg; Wouters-Ballman, P; Donnay, I et al

in Proceedings of the 11th International Congress on Animal Reproduction and Artificial Insemination (1988)

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See detailSome factors affecting successful vitrification of mouse blastocysts.
Van Der Zwalmen, P.; Gaurois, B.; Ectors, Fabien ULg et al

in Theriogenology (1988), 30(6), 1177-83

The effects of temperature and exposure time to vitrification solutions on In vitro survival of mouse blastocysts were investigated. Blastocysts were first exposed for 10 min to vitrification Solution 1 ... [more ▼]

The effects of temperature and exposure time to vitrification solutions on In vitro survival of mouse blastocysts were investigated. Blastocysts were first exposed for 10 min to vitrification Solution 1 (VS1) containing 10% glycerol-20% 1,2 propanediol in phosphate buffered saline (PBS), then to vitrification Solution 2 (VS2) with 25 % glycerol-25% 1,2 propanediol for various periods either at room temperature or at 4 degrees C. At room temperature survival dropped quickly, while at 4 degrees C an increase in survival was observed. It is concluded that the viability of mouse blastocyts after vitrification is dependent on the temperature and duration of equilibration in vitrification solutions. [less ▲]

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See detailObtention de blastocystes après maturation et fécondation in vitro d'ovocytes de bovin: premiers résultats
Ectors, Fabien ULg; Van Der Zwalmen, P.; Touati, Kamal ULg et al

in Annales de Médecine Vétérinaire (1988), 132

74.4% of two cells cow embryos are following in vitro maturation and fertilisation. Some embryos developed in rabbit's oviducts to morula and blastocyst stages. They wer frozen and thawed before transfer ... [more ▼]

74.4% of two cells cow embryos are following in vitro maturation and fertilisation. Some embryos developed in rabbit's oviducts to morula and blastocyst stages. They wer frozen and thawed before transfer in receptors. [less ▲]

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See detailInduction of superovulation in cattle
Beckers, Jean-François ULg; Wouters-Ballman, P.; Viviers-Donnay, I. et al

Conference (1987, September 04)

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