Effet d'une préstimulation ovarienne en début de cycle sur la réponse au traitement de superovulation chez la vacheTouati, Kamal ; ; Ectors, Fabien et alin Annales de Médecine Vétérinaire (1989), 133 In this study, we have induced superovulation in 67 heifers according to a known based treatment based on the administration of 32 mg (Armour Units) of FSH/LH (ratio LH/FSH=20%). The animals were divided ... [more ▼] In this study, we have induced superovulation in 67 heifers according to a known based treatment based on the administration of 32 mg (Armour Units) of FSH/LH (ratio LH/FSH=20%). The animals were divided in two groups: 30 control and 37 receiving 2.5mg (Armour units) of pure FSH on the third day and the fourth day of the estrous cycle. This pretreatment ("priming") was followed by a larger number of total (11.7 versus 6.4) and transferable (7.1 versus 3.6) embryos. This experiment demonstrates the efficiency of low doses of pure FSH administered 6 or 8 dyas before a superovulation in the final selection of ovarian follicles. [less ▲] Detailed reference viewed: 97 (10 ULg) Mise au point sur la folliculogenèseTouati, Kamal ; Ectors, Fabien ; Beckers, Jean-François et alin Annales de Médecine Vétérinaire (1989), 133 Folliculogenesis is usually divided in two stages: the first one going from the primordial follicle to the secondary or preantral, the second leading the preantral follicle to the preovulatory or Graafian ... [more ▼] Folliculogenesis is usually divided in two stages: the first one going from the primordial follicle to the secondary or preantral, the second leading the preantral follicle to the preovulatory or Graafian follicle. During the first period, follicular development is depending on intra ovarian factors mainly of growth-factors. However, many studies involve the FSH hormone in this period, when follicles have reached the 40 cells stage. During the second period, FSH plays an essential role in follicular growth and maturation. Regulatory process for FSH secretion and liberation depends mainly on inhibin. This fact is underlined in many species by typical FSH secretion profiles. The FSH levels can explain the ovarian control leading to one or to a limited number of preovulatory follicles according to the species characteristic (dominant follicle). [less ▲] Detailed reference viewed: 415 (7 ULg) Some factors affecting successful vitrification of mouse blastocysts.; ; Ectors, Fabien et alin Theriogenology (1988), 30(6), 1177-83 The effects of temperature and exposure time to vitrification solutions on In vitro survival of mouse blastocysts were investigated. Blastocysts were first exposed for 10 min to vitrification Solution 1 ... [more ▼] The effects of temperature and exposure time to vitrification solutions on In vitro survival of mouse blastocysts were investigated. Blastocysts were first exposed for 10 min to vitrification Solution 1 (VS1) containing 10% glycerol-20% 1,2 propanediol in phosphate buffered saline (PBS), then to vitrification Solution 2 (VS2) with 25 % glycerol-25% 1,2 propanediol for various periods either at room temperature or at 4 degrees C. At room temperature survival dropped quickly, while at 4 degrees C an increase in survival was observed. It is concluded that the viability of mouse blastocyts after vitrification is dependent on the temperature and duration of equilibration in vitrification solutions. [less ▲] Detailed reference viewed: 37 (0 ULg) Obtention de blastocystes après maturation et fécondation in vitro d'ovocytes de bovin: premiers résultatsEctors, Fabien ; ; Touati, Kamal et alin Annales de Médecine Vétérinaire (1988), 132 74.4% of two cells cow embryos are following in vitro maturation and fertilisation. Some embryos developed in rabbit's oviducts to morula and blastocyst stages. They wer frozen and thawed before transfer ... [more ▼] 74.4% of two cells cow embryos are following in vitro maturation and fertilisation. Some embryos developed in rabbit's oviducts to morula and blastocyst stages. They wer frozen and thawed before transfer in receptors. [less ▲] Detailed reference viewed: 77 (7 ULg) Induction of superovulation in cattleBeckers, Jean-François ; ; et alConference (1987, September 04) |
||